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Using a decade of administrative data from the Government of Canada, we provide fresh analysis of the composition and distribution of staff most formally associated with policy work, the Economics and Social Science (EC) classification. Comparative analysis across unit levels including 'ministerial departments' and central agencies, as well as non-standard organizations support but clarify the nature of the uneven distribution of policy analytical capacity across government. We demonstrate a dramatic increase in not only the overall complement of EC staff over time, particularly since 2017, but also significant growth at senior levels while junior EC staff have remained stable or declined. The findings also point to new dynamics related to the pace, orientation, and distribution of policy analytical capacity as governments gain, lose, and exercise that capacity often in the face of tough choices about how, where, and when to deploy policy resources. A partir d'une decennie de donnees administratives du gouvernement du Canada, nous formulons une nouvelle analyse de la composition et de la repartition du personnel le plus formellement associe au travail d'elaboration des politiques, la classification Economique et services de sciences sociales (EC). L'analyse comparative entre les differents niveaux d'unites, y compris les << ministeres >> et les organismes centraux, ainsi que les organismes non conventionnels, soutient mais clarifie la nature de la repartition inegale de la capacite d'analyse des politiques dans l'ensemble du gouvernement. Nous prouvons une augmentation spectaculaire non seulement de l'effectif global du personnel d'EC au fil du temps, en particulier depuis 2017, mais egalement une forte croissance au niveau des hauts dirigeants, tandis que le personnel subalterne d'EC est reste stable ou a diminue. Les resultats suggerent aussi de nouvelles dynamiques liees au rythme, a l'orientation et a la distribution de la capacite de formulation et d'analyse des politiques alors que les gouvernements gagnent, perdent et exercent cette capacite, souvent compte tenu des choix difficiles sur comment, ou et quand deployer les ressources politiques., INTRODUCTION Governments around the world are struggling with a variety of policy issues. Some are emergent like Covid-19 while others like economic productivity, social equality, and healthcare are longstanding. Part [...]

In this article, we argue that the entourage of ministerial advisers available to prime ministers and other ministers is an institutional power resource that can serve as a useful indicator to measure the changing nature of the political executive. Two novel contributions are made utilising four new datasets on ministerial advisers coupled with a comparative analysis of 21 governments in Australia, Britain, Canada and New Zealand, in varying dates between 1997 and 2020. First, by using ministerial advisers as a proxy indicator, we chart how the offices of executive politicians can either expand, remain stable or contract. As a corrective to the general long-term narrative that ministers' offices continually expand, our evidence shows this expansion has in some cases been interrupted and more generally manifests in different patterns from one government to the next. Second, we interrogate these patterns against the background of four typical assumptions from the party family, government tenure, parliamentary control and leadership stability literature. The new datasets, typology and analysis provide fresh comparative insights to advance our understanding about the evolving nature of the political executive in the four classic Westminster family countries. [ABSTRACT FROM AUTHOR]

Several Canadian and international scholars offer commentaries on the implications of the COVID-19 pandemic for governments and public service institutions, and fruitful directions for public administration research and practice. This first suite of commentaries focuses on the executive branch, variously considering: the challenge for governments to balance demands for accountability and learning while rethinking policy mixes as social solidarity and expert knowledge increasingly get challenged; how the policy-advisory systems of Australia, Canada, New Zealand, and United Kingdom were structured and performed in response to the COVID-19 crisis; whether there are better ways to suspend the accountability repertoires of Parliamentary systems than the multiparty agreement struck by the minority Liberal government with several opposition parties; comparing the Canadian government's response to the COVID-19 pandemic and the Global Financial Crisis and how each has brought the challenge of inequality to the fore; and whether the COVID-19 pandemic has accelerated or disrupted digital government initiatives, reinforced traditional public administration values or more open government. Plusieurs universitaires canadiens et internationaux ont offert des commentaires sur les implications de la pandemie du COVID-19 pour les gouvernements et les institutions de la fonction publique, ainsi que des orientations productives pour la recherche et la pratique en administration publique. Cette premiere serie de commentaires se concentre sur le pouvoir executif, en considerant de diverses facons : le defi pour les gouvernements d'equilibrer les exigences de responsabilite et d'apprentissage tout en repensant les combinaisons de politiques alors que la solidarite sociale et les connaissances d'experts sont de plus en plus remises en question; comment les systemes de consultation en politique de l'Australie, du Canada, de la Nouvelle-Zelande et du Royaume-Uni ont ete structures et mis en oeuvre en reponse a la crise du COVID-19; s'il existe de meilleures facons de suspendre les repertoires de responsabilite des systemes parlementaires que l'accord multipartite conclu par le gouvernement liberal minoritaire avec plusieurs partis d'opposition; comparer la reponse du gouvernement canadien a la pandemie du COVID-19 et a la crise financiere mondiale et comment chacune a mis le defi de l'inegalite au premier plan; et si la pandemie du COVID-19 a accelere ou perturbe les initiatives du gouvernement numerique, renforce les valeurs traditionnelles de l'administration publique ou un gouvernement plus transparent., Shaping the long shadows of COVID-19: three challenges for governments Arjen Boin and Paul't Hart Frantic mobilization efforts in the health sector, clear crisis communication and a remarkable degree of [...]

This article develops the concept of policy advisory system (PAS) management in recognition of the need to better theorise and empirically study how governments approach the complex systems of advice around them. In our analysis, we go beyond the conceptualisation of degrees of government's "control" over advisory sources. We use the dimensions of government agency and discretion and argue that PAS management falls into four forms: authoritative, dependent, laissez-faire, or absent. Using evidence from Australia, Canada, Britain, and New Zealand, we explore how governments operationalise these approaches through a range of choices and practices. The analysis points to the need to recognise that attempts to manage these systems occur both proactively and reactively with clear differences in the broad or narrow scope of management efforts. [ABSTRACT FROM AUTHOR]

The growing body of literature on digital governance, and policy advisory systems, often neglects the perspective of elected decision makers. What types of policy advice do ministers need in the digital governance era? Is digitization of government and policymaking reshaping supply and demand dynamics, the content of policy advice, or how advice is used within the policy process? This article borrows existing theoretical frames on digital governance to examine these and other questions from the perspective of ministers. The digital governance era will undoubtedly introduce novel disruptions and dilemmas, but it will also intensify longstanding tensions inherent in advisory work linked to questions of influence, capacity, and how well advisory systems adapt to serve ministers. We use information processing dynamics, policy entrepreneurship, and control and coordination to ground analysis of ministers' advisory demand in the digital governance era. We highlight unique considerations that apply to ministers as well as reflection on their place within the broader context of advisory work. We employ vignettes from the perspective of ministers throughout the article as a useful way to personalize the digital era disruptions and dilemmas particular to ministers. Le point de vue des decideurs elus est souvent neglige par l'ensemble croissant d'ouvrages portant sur la gouvernance numerique et les systemes de consultation sur la politique. De quels types de conseils strategiques les ministres ontils besoin a l'ere de la gouvernance numerique? Est-ce que la numerisation du gouvernement et de la prise de decisions remodele les dynamiques de l'offre et de la demande, le contenu des conseils en matiere de politique, ou la maniere dont la consultation est utilisee au sein du processus de politiques? En empruntant les cadres theoriques existants sur la gouvernance numerique, cet article etudie ces questions parmi d'autres, du point de vue des ministres. L'ere de la gouvernance numerique engendrera sans nul doute de nouveaux dilemmes et perturbations; elle intensifiera aussi les tensions de longue date qui sont inherentes au travail consultatif et liees aux questions d'influence, de competences, et a la maniere dont les systemes consultatifs s'adaptent pour servir les ministres. Afin de baser l'analyse de demande consultative des ministres a l'ere de la gouvernance numerique, nous utilisons les dynamiques du traitement de l'information, l'entrepreneuriat en politiques, le controle et la coordination. Nous soulignons les considerations particulieres qui s'appliquent aux ministres, ainsi que la reflexion sur la place qu'elles occupent au sein du contexte elargi du travail co l'optique des ministres afin d'aider a personnaliser les perturbations et les dilemmes causes par l'ere numerique et concernant particulierement les ministres., Introduction It is late Friday afternoon. Public servants and political staff around the table are grumpy. This is the umpteenth version of this briefing and they are still wrestling between [...]

This article provides fresh empirical insights into the evolving nature of policy analytical capacity in Australia, Britain, Canada, and New Zealand. We argue for a focus on the formal policy staff employed in government to provide greater conceptual clarity and empirical purchase regarding specific types of policy analytical resources linked to capacity. Using ten years of administrative data we chart the evolution of the size, composition, and distribution of formal public service policy staff in these cases. We find, contrary to expectations, that formal policy staff have increased in all four cases. Comparative analysis reveals not only uneven staffing capacity across government organizations, but also important developments in the seniority and distribution of staff in “analysts” and “managers” classifications. The findings are used to generate country profiles that demonstrate more nuanced patterns of policy analytical capacity change over time. The findings also raise important questions about shortcomings in how governments track and classify their policy staff, and their ability to effectively manage policy analytical resources in policy making. [ABSTRACT FROM AUTHOR]

This exploratory article examines the contemporary state of formal government policy staff in Australia, Britain, Canada, and New Zealand, contributing to the ongoing research seeking to understand the policy capacity of governments. It analyzes the size, composition, and distribution of the countries' formal policy staff across government units. Comparative analysis reveals significant differences in the basic organizing approaches used to categorize policy staff as well as important differences in their numbers, composition, and distribution across government units. While uneven distributions characterize all four cases, there is significant variance in where staff work, their seniority, and functional classifications as "analysts" or "managers". The analysis provides fresh insights into the nature and availability of policy analytical capacity within these governments. It also underscores major gaps in how governments collect and make available basic data on their policy staff, which raises questions about their ability to effectively manage and deploy those staff. [ABSTRACT FROM AUTHOR]

This article examines the infrastructure policy sector's capacity to respond to climate change adaptation through an analysis of the Canadian case. It includes a three-level examination of capacity: at the macro level through a virtual policy network analysis; at the meso level through examination of the lead department's evolving mandate and resources; and at the micro level through analysis of survey data related to departmental workers policy tasks and attitudes. Four hypotheses across these three levels are set out and tested at the national and subnational levels. Together, the findings suggest that the policy capacity in the Canadian infrastructure sector will be unable to meet the demands placed upon the sector to respond to the increasing challenges of climate change adaptation. KEY WORDS: infrastructure policy, Canada, federal-provincial relations, climate change, policy capacity, organizational routines, policy change, Introduction Forecasted increases in frequency and intensity of extreme weather events and climate variability have direct effects on the average conditions in which most major infrastructure operates (Auld, 2008; Auld [...]

This special issue contributes to extant empirical scholarship assessing governmental capacity to meet significant policy challenges, in this case those related to climate change adaptation. The study includes detailed examination of five policy sectors---finance, infrastructure, energy, forestry, and transportation--in two countries, Canada and the United States--in order to determine what kinds of governance arrangements and analytical capacities exist in this area, how they are changing (if at all), and how they interrelate with the status and evolution of climate change outcomes in each sector. The articles provide a comprehensive sampling of policy network structure and behavior, organizational mandates and resources, and actual job duties and training of policy actors across these sectors at both the federal and subnational level of government. KEY WORDS: policy capacity, climate change, climate change adaptation, comparative public policy, policy networks, organizational mandates, policy work, Introduction: Shifts in Climate Change Policy Mandates and Their Evaluation As governments shift from climate change mitigation efforts to approaches aimed at adaptation, the policy design and implementation challenges they [...]

Design thinking has become a popular approach for governments around the world seeking to address complex governance challenges. It offers novel techniques and speaks to broader questions of who governs, how they govern, and the limits of rational instrumentalism in policy making. Juxtaposing design thinking with an older tradition of policy design, this article offers the first critical analysis of the application of design thinking to policy making. It argues that design thinking does not sufficiently account for the political and organizational contexts of policy work. Design thinking also errs in universally privileging one particular policy style over others, and fails to account for the reality of policy mixes. Despite these deficiencies, it is argued that design thinking can inform and enrich governance by helping policy designers produce more adaptable designs, better appreciate the behavioral dynamics of public sector design, and leverage networked approaches to social problem solving. [ABSTRACT FROM AUTHOR]

This article explores the opportunities, challenges and expectations that the digital context of design and new digital era policy instruments, such as open data, big data, robotics and crowdsourcing, present to policy designers operating in today's governments. Adopting an instruments perspective, the article identifies four characteristics of digital era policy design before detailing three issues that must be addressed to ascertain the promise and pitfalls of digital era policy design. First, we argue that scholars and practitioners alike must investigate how traditional constraints on the ideal of rational, evidence-driven policy design-long acknowledged in the 'analogue design' literature-will fade or be exacerbated in the digital age. The article next confronts the reality that new digital designs and instruments will rarely emerge in a policy vacuum, but rather will be layered onto existing policy designs and complex policy mixes with sticky legacies that will be more or less amenable to digital design approaches. Finally, the article argues that while scholars and practitioners have been quick to presume that the digital age will foster a more collaborative model of state-to-non-state cooperation, digital era policy design may ultimately marginalize or eliminate citizens altogether from the design process, raising important questions about the as of yet little understood democratic implications of digital era policy design. [ABSTRACT FROM AUTHOR]

Garlic, Allium sativum, is broadly used around the world for its numerous culinary and medicinal uses. Wild garlic, Allium vineale, has been used as a substitute for garlic, both in food as well as in herbal medicine. The present study investigated the chemical compositions of A. sativum and A. vineale essential oils. The essential oils fromthe bulbs of A. sativum, cultivated in Spain, were obtained by three different methods: laboratory hydrodistillation, industrial hydrodistillation, and industrial steam distillation. The essential oils of wild-growing A. vineale from north Alabama were obtained by hydrodistillation. The resulting essential oils were analyzed by gas chromatography-flame ionization detection (GC-FID) and gas chromatography-mass spectrometry (GC-MS). Both A. sativum and A. vineale oils were dominated by allyl polysulfides. There were minor quantitative differences between the A. sativum oils owing to the distillation methods employed, as well as differences from previously reported garlic oils from other geographical locations. Allium vineale oil showed a qualitative similarity to Allium ursinum essential oil. The compositions of garlic and wild garlic are consistent with their use as flavoring agents in foods as well as their uses as herbal medicines. However, quantitative differences are likely to affect the flavor and bioactivity profiles of these Allium species. [ABSTRACT FROM AUTHOR]

Recent case studies and large-Nsurvey evidence has confirmed long-suspected shortages of public sector “policy capacity”. Studies have found that government policy workers in various jurisdictions differ considerably with respect to types of policy work they undertake, and have identified uneven capacity for policy workers to access and apply technical and scientific knowledge to public issues. This suggests considerable difficulties for government’s ability to meet contemporary policy and governance challenges. Despite growing attention to these matters, studies have not examined the “elite” policy workers many governments recruit to address these capacity shortages. Using an established survey instrument, this study of two Canadian recruitment programs provides the first comparative analysis of elite policy recruits, as policy workers. Three research questions anchor the study: (1) What is the profile of these actors? (2) What types of policy work do “elite” policy analysts actually engage in? (3) How does their policy work compare by recruitment program? The article provides fresh comparative data on the nature of elite policy work and policy analytical capacity, but, more importantly, a crucial baseline for future comparative study of how elite recruitment may facilitate “supply-side” capacity gains expected from recruitment programs. [ABSTRACT FROM AUTHOR]

Empirical examinations of partisan advisers detail significant differences in their policy work, influence, and their patterns of interactions with other policy actors. This raises important implications for their potential contributions to policy failure avoidance. Using recent qualitative data from elite interviews in Canada, this study finds that advisers' policy work contributes to political policy failure avoidance as policy is developed. The findings help unpack the types and nature of policy-based resource exchanges that advisers undertake, through advisory and non-advisory forms of policy work, that strengthen political control and manage policy perceptions by other actors during policy development. [ABSTRACT FROM AUTHOR]

'Policy advisory systems' have been central to moving beyond individual actor considerations to assessments of the interactive effects of multiple interlocking sets of suppliers, in specific jurisdictions, that provide policy advice to policymakers. This article argues, while useful, the advisory system concept should be revised given that previous approaches animated by the location of supply and government control over it have been weakened. We argue for a 'second wave' of advisory system studies that: (1) reorients the unit of analysis from the public service to advisory systems themselves, (2) better contextualizes advisory system operation and dynamics based on the subsystems within which they operate, and (3) focuses on questions of why advisory system components combine in particular policy instances and with what effect. Using access and compatibility, we posit a typology of policy advisory networks and develop four archetypes of policy ideational compatibility. [ABSTRACT FROM AUTHOR]

Background: Germline DNA mutations that increase the susceptibility of a patient to certain cancers have been identified in various genes, and patients can be screened for mutations in these genes to assess their level of risk for developing cancer. Traditional methods using Sanger sequencing focus on small groups of genes and therefore are unable to screen for numerous genes from several patients simultaneously. The goal of the present study was to validate a 25-gene panel to assess genetic risk for cancer in 8 different tissues using next generation sequencing (NGS) techniques. Methods: Twenty-five genes associated with hereditary cancer syndromes were selected for development of a panel to screen for risk of these cancers using NGS. In an initial technical assessment, NGS results for BRCA1 and BRCA2 were compared with Sanger sequencing in 1864 anonymized DNA samples from patients who had undergone previous clinical testing. Next, the entire gene panel was validated using parallel NGS and Sanger sequencing in 100 anonymized DNA samples. Large rearrangement analysis was validated using NGS, microarray comparative genomic hybridization (CGH), and multiplex ligation-dependent probe amplification analyses (MLPA). Results: NGS identified 15,877 sequence variants, while Sanger sequencing identified 15,878 in the BRCA1 and BRCA2 comparison study of the same regions. Based on these results, the NGS process was refined prior to the validation of the full gene panel. In the validation study, NGS and Sanger sequencing were 100% concordant for the 3,923 collective variants across all genes for an analytical sensitivity of the NGS assay of >99.92% (lower limit of 95% confidence interval). NGS, microarray CGH and MLPA correctly identified all expected positive and negative large rearrangement results for the 25-gene panel. Conclusion: This study provides a thorough validation of the 25-gene NGS panel and indicates that this analysis tool can be used to collect clinically significant information related to risk of developing hereditary cancers. [ABSTRACT FROM AUTHOR]

This article argues appointed political staffs are important, yet understudied, mechanisms for core executive policy coordination. It suggests revisions to update core executive theory to better accommodate the increasingly systematic use of political staffs throughout core executives. An analytical framework for political staffs’ core executive policy coordination is set out to specify important distinctions related to the type, and nature, of their likely policy coordination. The article concludes by returning to six established modes of executive politics to update them by integrating the policy coordination functions of political staffs in each variant. [ABSTRACT FROM AUTHOR]

Background Conventional Sanger sequencing reliably detects the majority of genetic mutations associated with hereditary cancers, such as single-base changes and small insertions or deletions. However, detection of genomic rearrangements, such as large deletions and duplications, requires special technologies. Microarray analysis has been successfully used to detect large rearrangements (LRs) in genetic disorders. Methods We designed and validated a high-density oligonucleotide microarray for the detection of gene-level genomic rearrangements associated with hereditary breast and ovarian cancer (HBOC), Lynch, and polyposis syndromes. The microarray consisted of probes corresponding to the exons and flanking introns of BRCA1 and BRCA2 (≈1,700) and Lynch syndrome/polyposis genes MLH1, MSH2, MSH6, APC, MUTYH, and EPCAM (≈ 2,200). We validated the microarray with 990 samples previously tested for LR status in BRCA1, BRCA2, MLH1, MSH2, MSH6, APC, MUTYH, or EPCAM. Microarray results were 100% concordant with previous results in the validation studies. Subsequently, clinical microarray analysis was performed on samples from patients with a high likelihood of HBOC mutations (13,124), Lynch syndrome mutations (18,498), and polyposis syndrome mutations (2,739) to determine the proportion of Lrs. Results Our results demonstrate that LRs constitute a substantial proportion of genetic mutations found in patients referred for hereditary cancer genetic testing. Conclusion The use of microarray comparative genomic hybridization (CGH) for the detection of LRs is well-suited as an adjunct technology for both single syndrome (by Sanger sequencing analysis)and extended gene panel testing by next generation sequencing analysis. Genetic testing strategies using microarray analysis will help identify additional patients carrying LRs, who are predisposed to various hereditary cancers. [ABSTRACT FROM AUTHOR]

The unfolded protein response (UPR) is a collection of pathways that maintains the protein secretory pathway during the many physiological and pathological conditions that cause stress in the endoplasmic reticulum (ER). The UPR is mediated in part by Ire1, an ER transmembrane kinase and endoribonuclease that is activated when misfolded proteins accumulate in the ER. Ire1's nuclease initiates the cytosolic splicing of the mRNA encoding X-box binding protein (Xbp1), a potent transcription factor that then upregulates genes responsible for restoring ER function. This same nuclease is responsible for the degradation of many other mRNAs that are localized to the ER, through Regulated Ire1 Dependent Decay (RIDD). Here we show that Smt3, a homolog of small ubiquitin-like modifier (sumo), is a non-canonical RIDD target in Drosophila S2 cells. Unlike other RIDD targets, the sumo transcript does not stably associate with the ER membrane, but instead relies on an Xbp1-like stem loop and a second UPR mediator, Perk, for its degradation during stress. [ABSTRACT FROM AUTHOR]

This special issue contributes to extant empirical scholarship assessing governmental capacity to meet significant policy challenges, in this case those related to climate change adaptation. The study includes detailed examination of five policy sectors-finance, infrastructure, energy, forestry, and transportation-in two countries, Canada and the United States-in order to determine what kinds of governance arrangements and analytical capacities exist in this area, how they are changing (if at all), and how they interrelate with the status and evolution of climate change outcomes in each sector. The articles provide a comprehensive sampling of policy network structure and behavior, organizational mandates and resources, and actual job duties and training of policy actors across these sectors at both the federal and subnational level of government. [ABSTRACT FROM AUTHOR]

Copyright of Canadian Political Science Review is the property of Canadian Political Science Review and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Canadian Political Science Review is the property of Canadian Political Science Review and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The article discusses the exploration of the use of nodality based information policy tools by electoral agencies in Canada. It mentions that those tools aimed at general and targeted communication political campaigns remain the most utilized policy tool as indicated in a review. It notes that those agencies are involved in frequent partnership and collaborative activities with other organizations in their policy community especially during elections.

The invasive algae in the Mediterranean Caulerpa taxifolia and Caulerpa racemosa var. cylindracea have gained much interest in the Adriatic Sea due to their fast spread and negative impacts on native seaweed species. Lack of accurate mapping and its overall high costs represent some of the major difficulties for successful detection and subsequent environmental management. Remote sensing techniques couM provide a quicker, more accurate and more cost efficient approach to mapping these invasive species. In order to allow for classification of remotely sensed data, we have collected and analyzed hyperspectral reflectance profiles of both invasive Caulerpa species in the Adriatic and the native Caulerpa cf. laetevirens species from the southeastern Atlantic coast of the United States. We have measured distinctive spectral features for spectroscopically distinguishing between these species, which is particularly interesting in the areas where these native and invasive species coexist. The initial results suggest that it might be possible to distinguish between the species based on their spectral reflectance profile. These results wouM have to be verified with samples taken from more populations in different phenological phases of the seaweed growth and other environmental conditions. [ABSTRACT FROM AUTHOR]

The Blood Profiling Atlas in Cancer (BLOODPAC) Consortium is a collaborative effort involving stakeholders from the public, industry, academia, and regulatory agencies focused on developing shared best practices on liquid biopsy. This report describes the results from the JFDI (Just Freaking Do It) study, a BLOODPAC initiative to develop standards on the use of contrived materials mimicking cell-free circulating tumor DNA, to comparatively evaluate clinical laboratory testing procedures. Nine independent laboratories tested the concordance, sensitivity, and specificity of commercially available contrived materials with known variant-allele frequencies (VAFs) ranging from 0.1% to 5.0%. Each participating laboratory utilized its own proprietary evaluation procedures. The results demonstrated high levels of concordance and sensitivity at VAFs of >0.1%, but reduced concordance and sensitivity at a VAF of 0.1%; these findings were similar to those from previous studies, suggesting that commercially available contrived materials can support the evaluation of testing procedures across multiple technologies. Such materials may enable more objective comparisons of results on materials formulated in-house at each center in multicenter trials. A unique goal of the collaborative effort was to develop a data resource, the BLOODPAC Data Commons, now available to the liquid-biopsy community for further study. This resource can be used to support independent evaluations of results, data extension through data integration and new studies, and retrospective evaluation of data collection., (Copyright © 2023 Association for Molecular Pathology and American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)

Background: Common sage ( Salvia officinalis ) is a popular culinary and medicinal herb. A literature survey has revealed that sage oils can vary widely in their chemical compositions. The purpose of this study was to examine sage essential oil from different sources/origins and to define the possible chemotypes of sage oil. Methods: Three different samples of sage leaf essential oil have been obtained and analyzed by GC-MS and GC-FID. A hierarchical cluster analysis was carried out on 185 sage oil compositions reported in the literature as well as the three samples in this study. Results: The major components of the three sage oils were the oxygenated monoterpenoids α-thujone (17.2-27.4%), 1,8-cineole (11.9-26.9%), and camphor (12.8-21.4%). The cluster analysis revealed five major chemotypes of sage oil, with the most common being a α-thujone > camphor > 1,8-cineole chemotype, of which the three samples in this study belong. The other chemotypes are an α-humulene-rich chemotype, a β-thujone-rich chemotype, a 1,8-cineole/camphor chemotype, and a sclareol/α-thujone chemotype. Conclusions: Most sage oils belonged to the "typical", α-thujone > camphor > 1,8-cineole, chemotype, but the essential oil compositions do vary widely and may have a profound effect on flavor and fragrance profiles as well as biological activities. There are currently no studies correlating sage oil composition with fragrance descriptions or with biological activities., Competing Interests: No funding was received for the conduct of this project; the authors declare no conflicts of interest.

Background : The chemical composition and bioactivities of essential oils (EOs) of fingered citron ( Citrus medica L. var. sarcodactylis (Noot.) Swingle) are considerably sensitive and lapsible during high-temperature processing of traditional separating techniques. In the present research, vacuum distillation and ultrafiltration were utilized in order to process the concentrated juice from fingered citron, obtaining a high-quality essential oil. Methods : In order to compare the essential oils obtained by conventional means, the chemical compositions of the essential oils were analyzed using GC-MS, before antimicrobial and antioxidant screening assays were carried out. Results : Oil which had been subjected to vacuum distillation was shown to maintain most of the distinctiveness of the fingered citron, due to its high content of characteristic flavor components and low content of cyclic oxygenated monoterpenoids. Interestingly, the oil obtained by ultrafiltration showed notable in vitro antimicrobial activity. The DPPH· radical-scavenging assay method revealed that the antioxidant abilities were as follows, presented in descending order: vacuum distillation oil > hydrodistillation oil > ultrafiltration oil. Conclusions : The essential oil obtained by vacuum distillation could be combined with the juice produced from fingered citron to create one of the most promising techniques in the fine-processing of citron fruits.

The chemotaxonomy of the Conocephalum spp. complex, based on GC-MS analysis of the volatile compositions, has helped to reveal cryptic biodiversity and delineate actual distribution patterns of chemotypes. In the Appalachian Mountains, two samples from eastern central part of the range were previously shown to.be C. salebrosum. Additionally, it has recently come to light that stress can alter the volatile composition of C. conicun. This study address a previously unsampled region of the southeastern Appalachians, a region that is a biodiversity epicenter, to determine if more chemotypic diversity remains to be seen for the Conocephalun spp. complex. A common garden experiment was performed, but yielded more of a common stress experiment, and significantly altered the volatile compositions. Wild-collected controls and a meta-analysis of these data and those from previous works suggest that the common garden experiment caused stress and that the liverworts sampled belong to the C. salebrosin clade of of the Conocephalum spp. complex.