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ABSTRACT Adding plant extracts to sausage and other meat products is very important to improve their quality, safety, and durability. The aim of this study was to evaluate the microbiological properties of beef sausage enriched with roselle (Hibiscus sabdariffa L.) sepal extract. The total content of phenolic and flavonoid compounds in hydroalcoholic extract of Roselle sepals was measured by Folin‐Ciocalteu test and aluminum chloride colorimetric method, and antioxidant activity was measured by DPPH method. Pectin was used to encapsulate the extract. The characteristics of capsules, including particle size, zeta potential, and capsule efficiency were measured. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of extracts were performed by tube dilution method. Microbial tests were performed on sausage treatment samples. Analysis of the obtained data was done using SPSS and Excel software. The study revealed that the total phenolic and flavonoid compounds in the extract were 174.6 mg of gallic acid per gram and 16.8368 mg of quercetin, respectively. The extract displayed the highest antioxidant activity at elevated concentrations. Particle size ranged from 16.833 to 640.534 μm. The zeta potential and capsule efficiency were found to be satisfactory. Compared to the encapsulated extract, the free roselle extract better inhibited Staphylococcus aureus (s. aureus), Salmonella typhimurium (S. typhimurium), and Escherichia coli (E.coli). During storage until day 20, these bacteria did not grow in the free or encapsulated extract groups. However, S. aureus appeared in the free extract group from day 25 and the encapsulated extract group from day 20. Roselle extract has antimicrobial properties that can improve the quality and safety of beef sausage during storage. [ABSTRACT FROM AUTHOR]

Pure CeO2 and Ce1‐xMnxO2, where x = 0, 2, 4 and 6% w/w, were synthesized by sol‐gel method using Acacia Concinna fruit extract as a surfactant and stabilizing agent. The synthesized particles were subjected to various characterization techniques, including XRD, HRTEM, FESEM, FTIR, Raman and UV‐Visible spectroscopy. The XRD pattern of the samples demonstrated single phase of CeO2, with cubic fluorite type structure and crystallite size of 6–7 nm. The morphology of the nanoparticles (NPs) was analyzed by FESEM and HRTEM which illustrated an aggregate of irregular spherical nanoparticles and the average particle size is around 18 nm. Raman spectroscopy validated the phase purity and indicated about the structural defects caused by the dopants. The bandgap energy of CeO2 NPs is calculated to be 3.00 eV from the Tauc plot of UV‐Visible spectroscopy, which gradually decreased to 2.43 eV with increase in manganese doping. Cytotoxicity studies signified the role of Mn‐doped CeO2 NPs as a potential nanotherapeutic agent for cancer treatment. The cell viability assay showed that 200 µg/mL of 6% Mn‐doped CeO2 nanoparticles exhibited remarkable cytotoxic effect by inhibiting 50% of cancer cells both in breast and colon cancer cell lines without affecting the healthy cell lines. [ABSTRACT FROM AUTHOR]

Background: Adult tendon tissue has limited and slow regenerative capacity. Sambucus nigra plant possesses antioxidant and anti‐inflammatory attributes. Objectives: This study aimed to evaluate the effect of hydroalcoholic extract of this plant's fruit on superficial digital flexor tendon repair in rabbits (SDFT). Methods: Twenty‐five male New Zealand white rabbits weighing 1.5–2 kg were selected, quarantined and randomly divided into four groups of six. By performing a partial left posterior limb tenotomy, differentiating the SDFT and creating multiple scrapes were performed. During 3 consecutive days post‐surgery, the positive control group was injected with 0.5 mg/kg dexamethasone, whereas the treatment groups received extract doses of 200 and 400 mg/kg, respectively. The negative control group did not receive any medication. Evaluation of sonographic and histopathological parameters was conducted on days 0, 7 and 28 post‐surgeries. Findings were analysed and compared using SPSS22. Results: Both treatment groups showed significant differences in echogenicity, and collagen fibre alignment compared to the control and positive control groups, in sonographic evaluation. Histopathological examination revealed fewer inflammatory cells and increased collagen fibre formation in the treatment groups compared to the other two groups. No significant difference in angiogenesis was observed among the groups on days 7 and 28 (p value <0.05). Conclusions: The results indicate that the S. nigra fruit extract, by stimulating collagen synthesis and reducing inflammation, effectively accelerates the healing process of injured tendons. [ABSTRACT FROM AUTHOR]

Studies were conducted focusing on the drying of chili pepper fruits (Capsicum annuum L.), cultivar Cyklon, using convective (AD), convective-microwave (AMD), vacuum (VD), and freeze-drying (FD) methods. The influence of the drying method and temperature on the kinetics of the process and selected quality attributes of the dried product were evaluated. It was demonstrated that the Midilli model best described the drying kinetics for all methods across the entire measurement range. FD and VD produced dried products with the highest brightness and the greatest value of the a* color parameter. The lowest b* color parameter was observed for the product dried using FD at 40 °C, while the highest b* value was noted for samples dried using AMD (100 W) at 60 °C. The highest carotenoid retention was achieved with the FD method at 40 °C, while the lowest carotenoid content was found in the product obtained using the AMD method (100 W) at 60 °C. The smallest losses of capsaicinoids were observed after FD drying at 40 °C, while the largest were found for AMD (100 W) at 60 °C. The analysis of chili pepper fruit extracts revealed the quantitative composition of 12 main phenolic compounds using the UHPLC-UV method. The highest polyphenol content was obtained with FD, while the lowest total polyphenol content was recorded after AD. Regardless of temperature, the total flavonoid content was highest in extracts from FD products, and the lowest flavonoid content was found after AMD at 100 W. For all drying methods analyzed, the total flavonoid content in the pepper extracts decreased with increasing temperature. [ABSTRACT FROM AUTHOR]

This study explores for the first time the impact of chitosan (CS) with varying molecular weights (MW), orange peel extract concentration, and hydroxypropyl methylcellulose (HPMC) content on the formulation of buccal tablets for treating oral infections. Utilizing a statistical design of experiments (DoE), nine different formulations were evaluated for mechanical properties, dissolution behavior, mucoadhesion, and biological activity. A formulation with high CS MW, 60% orange peel extract, and 8% HPMC, emerged as the optimal formulation, demonstrating superior tabletability, compressibility, and compactibility. Dissolution studies indicated that hesperidin release followed the Higuchi model, with higher extract content enhancing this phenomenon. Mucoadhesion improved with increased HPMC and CS concentrations, although higher extract content reduced bioadhesion. Biological assays showed that higher extract levels boosted antioxidant activity, while CS primarily contributed to anti-inflammatory effects. The optimized formulation exhibited broad antimicrobial activity against key oral pathogens, surpassing the effectiveness of the individual components. Principal component analysis (PCA) further confirmed the significant influence of extract content on tablet properties. These findings suggest that the optimized tablet formulation holds promise for effective buccal delivery in the treatment of oral infections, warranting further investigation in clinical settings. [ABSTRACT FROM AUTHOR]

This investigation examined the potential antibacterial and antidiabetic effects of wound dressings created using electrospun nanofibers containing Ziziphus jujuba fruit extract (ZJ). These nanofibers were composed of a combination of Polycaprolactone (PCL), Polyvinyl Alcohol (PVA), and Polyhexamethylene Biguanide (PHMB). The process of creating these nanofibers involved electrospinning. The nanofiber products, which included PCL, PCL/PVA, PCL/PVA/ZJ, PCL/PVA/PHMB, and PCL/PVA/PHMB/ZJ, underwent a morphology, physicochemical, and biological assessment. Incorporating PHMB into the nanofibers enhanced the antibacterial properties, effectively preventing bacterial infections in wounds. Furthermore, including ZJ fruit extract in the nanofibers provided antidiabetic properties, making these dressings suitable for diabetic patients. The PCL/PVA/PHMB/ZJ combination exhibited exceptional healing capabilities and superior antibacterial efficiency in MRSA-infected wounds. The histological assay confirmed complete wound healing by day 14, accompanied by reduced inflammation. Based on these findings, using PCL/PVA/PHMB/ZJ as innovative wound dressings is recommended, as they can expedite wound healing while offering significant antidiabetic and antibacterial features. Ultimately, these electrospun nanofibers possess the potential to serve as advanced wound dressings with enhanced antibacterial and anti-diabetes properties. [ABSTRACT FROM AUTHOR]

This study investigated the effect of high-pressure enzymatic treatment (HPET) on the quality characteristics such as soluble solid content, yield, flavonoid content, and phenolic acid content of mulberry fruits. Of particular note, first, the quality characteristics of the mulberry fruits were most significantly improved under the high-pressure treatment with mixture of Pectinex Be XXL and Ultra color. Second, in the flavonoids, a decrease in rutin content and increases in hyperoside and quercetin contents were confirmed by HPET, which may be the result of the two-step deglycosylation of rutin by pectinase activity. Finally, the extraction yield of mulberry fruits was maintained during HPET 0–36 h, and its bioactive compound profile was variously changed. Therefore, this study provided new clues as to how the HPET may promote changes in the extraction yield and bioactive substance profile of mulberry fruits and suggest the potential for use of natural materials based on mulberry fruit extracts. [ABSTRACT FROM AUTHOR]

Background: Between food and medicine, nutraceuticals are widely used in human health for the prevention and treatment of various diseases. This study aims to determine the cytoprotective effects of Anethum gravelons fruit extract (AGFAE) on castor oil‐induced diarrhea in rats due to its phytochemical and antioxidant properties. Methods: Male rats were divided into six groups of six animals each: Control (C), Castor oil (CO), CO + different doses of AGFAE (50, 100, and 200 mg/kg, b.w., p.o.), and the CO + loperamide group (LOP, 10 mg/kg, b.w., p.o.). Key Results: In vitro, the chemical composition of aqueous Dill fruit extract showed strong antioxidant activity, with a high content of total polyphenols, flavonoids, and tannins. In our in vivo studies, pre‐treatment with AGFAE reduced malondialdehyde and hydrogen peroxide levels and maintained normal activity of enzymatic and non‐enzymatic antioxidants in the gastric and intestinal mucosa. In addition, we found that AGFAE prophylaxis improved the stability of many plasma biochemical parameters altered by castor oil intoxication, such as C‐reactive protein concentrations and alkaline phosphatase activities. Conclusions & Inferences: We suggest that AGFAE phenolic compounds had significant protection against diarrhea involving several mechanisms such as reducing hypersecretion, peristaltic, inflammation, and preserving the endogenous antioxidant levels. [ABSTRACT FROM AUTHOR]

Calabash (Crescentia cujete) is a plant that grows in tropical climates and is scattered and easily found in Indonesia. Calabash is also known to contain several secondary metabolite compounds that have the potential as an antioxidant. This research is purposed to identify phytochemical compounds in calabash fruit seed extract, identify compounds based on the result of LC-MS, and know the antioxidant activity of calabash fruit seeds. The samples were extracted using the maceration method, and then phytochemical screening, LC-MS tests, and antioxidant tests were continued using the DPPH method. This research showed that the ethanol extract of calabash seed positive contains saponin, flavonoids, and triterpenoid compounds. Ethyl acetate extract of calabash seed contains flavonoids, alkaloids, and triterpenoids. The n-hexane extracts positively contain tannins, alkaloids, and steroids. The values of antioxidant activity of ethanol, ethyl acetate, and n-hexane extracts were IC50 = 29.7883, 7.219, and 848.712 ppm (µg/ml), respectively. Ethyl acetate extract as the best antioxidant activity was then tested with LC-MS, and results obtained from 26 compounds with the composition of the largest % area is convolvidine (11.277%), coumaroylquinic acid (8.290%) and taxchinin G (8,233%). They showed that calabash fruit seed extract has very strong antioxidant activity, with the best activity being the ethyl acetate extract of calabash fruit seed. [ABSTRACT FROM AUTHOR]

Freezing-thawing procedures and semen manipulation for in vitro fertilization induce oxidative stress, which in turn leads to impaired sperm quality. The aim of this study was to evaluate whether incubation of frozen-thawed buffalo semen with olive fruit extracts (OFE), known to contain a high concentration of phenolic antioxidants, would improve semen quality by reducing oxidative stress. Frozen sperm (4 ejaculates/4 bulls/3 replicates) were thawed and diluted to 30 × 106/mL in IVF medium with 0, 72, 143, and 214 μL/mL of OFE, corresponding to 0 (D0-control), 50 (D50), 100 (D100), and 150 (D150) μM hydroxytyrosol. Sperm viability, acrosome integrity, membrane functionality, motility, and sperm kinetics were evaluated immediately after thawing (T0) and after 1 (T1) and 2 h (T2) of incubation at 38.7 °C. Based on the results, sperm biological antioxidant potential (BAP) and ROS levels (ROMs) were assessed in D0 and D100 groups at T1 and T2. To assess the effect of OFE on fertilizing ability, heterologous penetration rates were also evaluated, using bovine abattoir-derived oocytes. The treatment with OFE at all concentrations tested increased (P < 0.05) the percentage of acrosome intact spermatozoa compared to the D0-control at T1, but the effect was more evident (P < 0.01) with D100 (54.5 ± 3.0, 60.5 ± 1.5, 65.2 ± 3.3, and 62.5 ± 1.7, with D0, D50, D100, and D150 OFE, respectively). Total motility, progressive motility, rapid velocity, and progressive velocity decreased (P < 0.05) at T2 only in the D0-control group. The percentage of rapidly progressive sperm and the progressive motility tended to increase (P < 0.10) at T1 and T2, respectively, in D100 compared to D0 (24.7 ± 4.1 vs 16.4 ± 1.6 and 22.8 ± 2.7 vs 17.0 ± 1.2, respectively). The treatment with D100 OFE of frozen-thawed sperm increased (P < 0.05) some kinetic parameters (VAP and WOB). Spermatozoa incubated with D100 OFE exhibited higher (P < 0.01) total and normospermic oocyte penetration rates compared to D0 (86.5 ± 1.4 vs 78.5 ± 0.7, and 70.6 ± 1.5 vs 63.8 ± 1.1, respectively). Additionally, D100 OFE increased sperm BAP concentrations at both T1 and T2, while ROS levels were unaffected. These results suggest that incubating frozen-thawed buffalo semen with OFE is an effective strategy for preserving semen quality and in vitro fertilization ability by enhancing sperm antioxidant capacity. • Olive fruit extract supplementation enhanced in vitro fertilization ability of buffalo sperm. • The addition of olive fruit extract improves buffalo sperm biological antioxidant potential. • The treatment with olive fruit extract increased the percentage of acrosome intact spermatozoa. [ABSTRACT FROM AUTHOR]

Rosemary is a plant from the Lamiaceae family with antioxidant properties, Therefore, the purpose of this study was to extract rosemary plant extract using different solvents, methods (maceration, hot extraction, Soxhlet, percolation and sonication), particle size (300, 500 and 800 μm) and plant to solvent ratios (1:100, 2:100, 3:100, 4:100 & 5:100) were. In this experimental study, the amount of phenolic and flavonoid compounds was measured by aluminum chloride colorimetric method and the antioxidant activity of plant extracts was evaluated by DPPH method. To extract these compounds, different solvents and methods were used and compared and analyzed and Data analysis was performed using SPSS software and ANOVA test. The best solvents for the extraction of phenolic, flavonoid and antioxidant compounds were water -methanol (20:80). The amount of total phenolic compounds using this solvent was 7.172 (mg/g) and flavonoid compounds were 28.157 (mg/g) and the antioxidant activity to inhibit free radicals was 87.2586 (mg/lit). The best method for extracting phenolic compounds was Maceration method with a rate of 7.481 (mg/g) and for flavonoid compounds with a rate of 47.85 (mg/g) and 73.524 (mg/lit) to inhibit free radicals. The results show that in order to achieve the maximum extraction of total phenolic compounds, flavonoids and antioxidants in rosemary extract, use plant powder with a particle size of 300 μm, water solvent: methanol (80:20), plant to solvent ratio 1:100 (g/ml) and the use of maceration method should be used as optimal operating conditions. [ABSTRACT FROM AUTHOR]

The enzyme-assisted approaches for plant phenolics extraction are more eco-friendly methods compared to acid or alkaline hydrolysis. Carbohydrase enzymes can release free phenolics from plant materials by cleaving the glycosidic bonds between phenolic compounds and cell wall polymers. In this study, the efficiency of carbohydrase-assisted treatment approaches was evaluated to extract bioactive phenolics from hawthorn (Crataegus orientalis) fruit residues. Enzymatic treatment of the fruits was operated by using a crude cellulolytic enzyme cocktail from Rhizomucor miehei NRRL 5282 and a pectinase preparate from Aspergillus niger. Both cellulase and combined cellulase–pectinase treatments improved the total phenolic content (TPC) and antioxidant activity of extracts. The TPC increased to 1899 ± 27 mg gallic acid equivalents/100 g dry matter during the combined enzyme treatment, showing a strong correlation with the average antioxidant capacity determined by ferric-reducing antioxidant power (1.7-fold increment) and 2,2-diphenyl-1-picrylhydrazyl (1.15-fold increment) reagents. The major phenolics in enzyme-treated extracts were vanillic and ferulic acids, the concentrations of which increased 115.6-fold and 93.9-fold, respectively, during carbohydrase treatment. The planktonic growth of Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Chromobacterium violaceum was slightly inhibited by the extracts with minimum inhibitory concentration values between 15.0 and 77.9 mg/mL, while the yeasts tested were quite resistant to the samples. B. subtilis and yeast biofilms were sensitive to the enzyme-treated extracts, which also showed quorum-sensing inhibitory effects against C. violaceum. The obtained bioactive hawthorn extracts hold potential as a natural source of antioxidants and antimicrobials. [ABSTRACT FROM AUTHOR]

Faster ripening and senescence of banana fruit is closely associated with higher ethylene production and oxidative damages. Present study evaluated the mechanism of sodium nitroprusside (as a nitric oxide donor) on delayed ripening and senescence in banana as well as mitigating the oxidative damage. Nitric oxide (NO) was applied to fruit by vacuum infiltration (1 mM) and stored for 8 days at 25 °C with 65–70% relative humidity. Fruit quality, electrolytic leakage, phenolic content, reactive oxygen species production and scavenging activity were investigated. The results showed that NO treated fruit retained higher phenolics, antioxidant capacity (radical scavenging activity), and reduced electrolytic leakage, malondialdehyde (MDA) content and free radical production (H2O2 and O2−) than control on the last day. The inhibitory effect of NO on ripening was observed with delayed chlorophyll loss as well as reduced ethylene and CO2 production rate in banana during storage. Therefore, vacuum infiltrated NO could be a possible postharvest treatment for mitigating the oxidative damages and preserving banana fruit up to 8 days at ambient condition. [ABSTRACT FROM AUTHOR]

Wound healing comprises an intricate process to repair damaged tissue. Research on plant extracts with properties to expedite wound healing has been of interest, particularly their ability to enhance the stemness of keratinocyte stem cells. Hence, the present study aims to determine the wound healing and stemness potentiation properties of an ethanolic extract derived from Cucurbita moschata fruit pulp (PKE). Human keratinocytes (HaCaT) and primary skin fibroblast cells were used in this study. The migration of the cells was examined by using a scratch wound healing assay, and spheroid behavior was determined by using a spheroid formation assay. The proteins related to migration and stemness were further measured by using Western blotting to explore the mechanism of action of PKE. The methods used to evaluate PKE's antioxidant properties were 2,2‐diphenyl‐2‐picrylhydrazyl (DPPH) scavenging, ABTS radical scavenging activity, and superoxide anion radical scavenging (SOSA) assays. The phytochemistry of the PKE was investigated using phytochemical screening and high‐performance liquid chromatography (HPLC) analysis. The results of this study indicate that nontoxic concentrations of PKE increase the rate of migration and spheroid formation. Mechanistically, PKE increased the expression of the migratory‐related protein active FAK (phosphorylated FAK), and the subsequence increased the level of p‐AKT. The expression of stem cell marker CD133, upstream protein signaling β‐catenin, and self‐renewal transcription factor Nanog was increased. The PKE also possessed scavenging properties against DPPH, ABTS, and SOSA. The phytochemistry analyses exhibited the presence of alkaloids, glycosides, xanthones, triterpenes, and steroids. Additionally, bioactive compounds such as ɑ‐tocopherol, riboflavin, protocatechuic acid, β‐carotene, and luteolin were detected. The presence of these chemicals in PKE may contribute to its antioxidant, stem cell potentiation, and wound‐healing effects. The findings could be beneficial in the identification of valuable natural resources that possess the capacity to be used in the process of wound healing through the potentiation of stemness via a readily detectable molecular mechanism. [ABSTRACT FROM AUTHOR]

The study aimed to investigate the therapeutic efficacy of S. dulcamara extract (SDE) in managing type II diabetes‐related parameters, including blood glucose levels, α‐amylase activity, and modulation of endogenous antioxidant enzymes (SOD, GPx, CAT). Diabetes was induced in rats using streptozocin, and the anti‐diabetics effect was evaluated by the administration of S. dulcamara extract at different doses. Blood glucose levels, α‐amylase activity, and endogenous antioxidants were estimated and found to be significant (P<0.05) in the rats treated with the extract of S. dulcamara at a dose of 200 mg/kg. The extract showed dose‐dependent inhibition of α‐amylase suggesting the potential benefits in glycemic control. Moreover, the study demonstrated a modulation in the activity of endogenous antioxidant enzymes (SOD, GPx, CAT). Importantly, the fruit extract showed no significant hemolysis or acute toxicity at varying concentrations (12.5 and 25 mg/mL) indicating the safe and biocompatible nature, as confirmed by normal biochemical parameters and histopathological examination. In conclusion, the methanolic fruit extract of S. dulcamara shows significant anti‐diabetic activity by alleviating oxidative stress associated with type II diabetes and its complications. [ABSTRACT FROM AUTHOR]

Eriobotrya japonica Lindl. (Loquat) fruit is a subtropical edible fruit originally from China. It grows well in Egypt, but it is not widely known. In the current study, the fruit was extracted with 80% ethanol to get the total ethanol extract (TEE). A part of which was fractionated by dichloromethane to yield polar and nonpolar fractions (PF and NPF). The antioxidant and anti‐inflammatory activities of the TEE were in vitro evaluated. The complete Freund's adjuvant (CFA) arthritis model was used to explore the in vivo biological assessment of the anti‐arthritic properties in vivo of the TEE, PF, and NPF of the fruit. Additionally, the inspected limbs detached from all animals were subjected to histological inspection. Moreover, GC/MS analysis of the unsaponifiable (USF) and saponifiable (SF) fractions of the NPF was performed. Furthermore, 64 metabolites from various chemical classes were identified using UHPLC/HR‐MS/MS analysis of the TEE of the fruit in both positive and negative ionization modes. The positive ionization mode of loquat fruit allowed for the first time the detection of two kinds of lyso‐glycerophospholipids (Lyso‐GPLs): lyso‐glycerophosphoethanolamines (Lyso‐PtdEtn) and lyso‐glycerophosphocholines (Lyso‐PtdCho). The fruit extracts exhibited a notable in vivo anti‐arthritic activity by decreasing paw thickness in the treated rats and adjusting the inflammatory mediators. The TEE showed the highest anti‐arthritic activity, followed by the PF that showed an observed activity, while the NPF exhibited the lowest activity. Histopathological findings showed a marked improvement in the arthritic condition of the excised limbs. Thus, E. japonica fruit may be considered as a promising natural antioxidant and anti‐arthritic agent. [ABSTRACT FROM AUTHOR]

Due to inadequate treatment, diabetes mellitus, which is characterized by hyperglycemia, is a global health challenge that requires creative management techniques. This study explores the therapeutic potential of Abelmoschus esculentus L. Moench fruit extracts in diabetes, offering an ample approach includes phytochemical analysis, qualitative and quantitative assessments, antidiabetic efficacy, antioxidant potential, and in vivo and in vitro investigations. Thin‐layer chromatography (TLC), high‐performance liquid chromatography (HPLC), and gas chromatography–mass spectrometry (GC–MS) were utilized to disclose the phytochemical profile of the fruit extracts, which indicated a diverse range of bioactive compounds. Four polyphenolic compounds including chlorogenic acid, quercetin, rutin, and morin were confirmed by HPLC fingerprinting, with rutin being the most prevalent. Qualitative analysis reveals the presence of carbohydrates, emodin, terpenoids, lignin, glycosides, and anthraquinones, while indicating the absence of amino acids, anthocyanins, phlobatannins, lactones, and leucoanthocyanins. Quantitative analysis reveals total phenolic, tannin, and flavonoid. In vitro assessments demonstrate the ability of the extracts to inhibit alpha amylase. Radical scavenging activities were evaluated through DPPH assay, underscoring their antioxidant capacity with high effect in methanol extracts. Additionally, the methanol extract decreased the blood glucose level of Type 2 diabetic mice. Notably, test samples stabilized blood glucose levels, reversed deviations in blood profile parameters, blood biochemistry, and regulated organ weight. Based on its antioxidant and antidiabetic effects, as well as positive effects on the physiology of Type 2 diabetic mice, A. esculentus emerges as an important nutraceutical vegetable. As an important nutraceutical vegetable, it holds promise for diabetes management. A. esculentus can be incorporated into dietary interventions for individuals with diabetes, harnessing its natural antioxidant and antidiabetic properties. Our findings validate the traditional use of A. esculentus in green therapeutics. In conclusion, A. esculentus emerges as a valuable ally in the fight against diabetes, bridging the gap between ancient wisdom and modern science. Further research and practical implementation are warranted to fully realize its potential. [ABSTRACT FROM AUTHOR]

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Purpose: This study was designed to study testicular-histopathology and lipid profile against 50ppm of fluoride (ad labitum) exposure in mice. Moreover, the curative potentials of vitamin E, Punica granatum and Olea europea fruit extracts were also studied. Methods: Five groups (10 animals/group) were treated as follow (a) Control (C) (F free water-15 days); (b) NaF {50ppm F-ions in drinking water (10days) and F free water (5days)}; (c) NaF + vitamin E (NaFE) {50ppm F-ions (10days) and F free water+60µg vitamin E in corn oil (5days)}; (d) NaF+ Punica granatum fruit extract (NaFP) {50ppm F ions (10days) and F free water+0.2mL P. granatum fruit extract (5days)}; (v) NaF+ Olea europea fruit extract (NaFO) {50ppm F-ions (10days) and F free water +0.2mL O. europea extract (5days)}; All animals were sacrificed on day 16 to obtain blood for lipid profile and testes for histopathological studies. Results: Results shows peculiar histopathological changes in the testis, including the loss of interstitial tissues, spermatogonia, spermatocytes, spermatids and maturing sperms. These signs were convincingly recovered in NaFO, followed by other two post treatment groups NaFP and NaFE. Similarly the micrometric data shows significant increase in the number of spermatogonia, spermatocytes and developing spermatozoa, in NaFO (CSA of seminiferous tubules, number of spermatogonia, spermatocytes:, sperms embedded in sertoli cells & number of dislodged sperms). The rise of High Density Lipoproteins (HDL) with a simultaneous decline in plasma cholesterol, Low Density Lipoprotein (LDL) and Very Low Density Lipoprotein (VLDL) in NaFO indicate the importance of these post treatments, against altered lipid profile of the F toxicology. Excellent testicular rehabilitation found in NaFO group that indicate the curative potential of O.europea against altered histopatology against F exposure in testes. Conclusions: The severity of the mentioned pathology of NaF group indicates that Olea europea and Punica granatum post treatment can effectively eradicate the toxic changes of F exposure in lipid profile, while Olea europea fruit extract showed more ameliorationative capability against F-exposure in testes. [ABSTRACT FROM AUTHOR]

ABSTRACT Green synthesis of metal nanoparticles using plant sources is one of the most environmentally sound, economically safer, and operationally simple approaches compared with their physiochemical methods. In this work, we have developed the biogenic synthesis of Ag nanoparticles using the aqueous peel extract of Punica granatum L. fruit, which mitigates the requirement for any hazardous reagents or toxic chemicals. The ultraviolet‐visible spectrum confirmed the formation of Ag@PPE NPs with an absorption peak at 420 nm. The X‐ray diffraction analysis confirms that the biosynthesized Ag@PPE NPs are crystalline, with a crystallite size of 9.23 nm. SEM and TEM images revealed the spherical morphology of Ag@PPE NPs, with particle sizes ranging from 2 to 20 nm. The biosynthesized Ag@PPE NPs were explored as antimicrobial agents against both Gram‐positive (CA‐MRSA) and Gram‐negative (Escherichia coli) bacteria as well as Candida albicans (ATCC 14053). The mean zone of inhibition against the CA‐MRSA group was 15.34 ± 2.5 mm, while it was 12.33 ± 1.5 mm against E. coli. In this study, Ag@PPE NPs demonstrated strong antibiofilm activity and antioxidant activity. Moreover, the catalytic applicability of the synthesized Ag@PPE NPs has been investigated for the oxidative hydroxylation of differently substituted aryl boronic acids into phenols at room temperature. The reaction proceeded efficiently in a short reaction time, and the desired products were obtained with high to excellent yields (82%–94%). Notably, the nanocatalyst can be recovered in five consecutive runs without decreasing its catalytic performance. The plausible mechanism of this ipso‐hydroxylation reaction is well presented. [ABSTRACT FROM AUTHOR]

Currently energy crisis is one of the challenging issues worldwide. As such, generation of green alternative energy should take the attention of researchers around the globe. This work reports synthesis of zinc oxide nanoparticles (ZnO NPs) using biological method (using fruit extract of Ruta chalepensis) for photoanode of dye sensitized solar cells (DSSCs). In the study, the performance of DSSCs derived from chemically synthesized and biologically synthesized ZnO NPs were evaluated. The plant extract templated synthesis of ZnO NPs were prepared in varied zinc nitrate to R. chalepensis fruit extract ratios of 1 : 2,1 : 1, and 2 : 1 and labeled as g‐ZnO(1 : 2), g‐ZnO(1 : 1) and g‐ZnO(2 : 1); and that of chemically synthesized ZnO NPs is labelled as c‐ZnO (as a control). Thermogravimetric analysis‐differential thermal analysis (TGA/DTA) reveals that ZnO NPs are stable above 450 °C. From X‐ray diffraction (XRD) pattern, average size of g‐ZnO(1 : 2), g‐ZnO(1 : 1), g‐ZnO(2 : 1) and c‐ZnO NPs were estimated to be 7, 13, 18 and 26 nm, respectively. Scanning electron microscopy (SEM) reveals the presence of ZnO in clustered, flake‐like, and some agglomerated spherical forms; and energy dispersive spectroscopy (EDX) confirms presence of Zn and O atoms in the sample. The optical band gaps of g‐ZnO (1 : 2), g‐ZnO(1 : 1), g‐ZnO(2 : 1) and c‐ZnO NPs were measured to be 3.30, 3.25, 3.23 and 3.22 eV respectively using ultraviolet‐visible (UV‐Vis) absorbance spectrophotometer. Fourier transform infrared (FT‐IR) spectroscopic analysis shows the absorption band for Zn−O bonding between 400 and 600 cm−1. The produced photoanode, crystal violet dye, quasi‐solid‐state electrolyte, and counter electrode were used to fabricate DSSCs. The power conversion efficiency (PCE) of the constructed DSSC device with a c‐ZnO photoanode is 1.95 %, and those with a g‐ZnO (2 : 1), g‐ZnO(1 : 1), and g‐ZnO(1 : 2) photoanodes have PCE of 1.9 %, 1.36 %, and 0.215 % respectively. [ABSTRACT FROM AUTHOR]

Antioxidants are compounds needed by the body to protect body cells from damage caused by free radicals. Free radicals can damage macromolecules such as cell membrane lipids, DNA, and proteins, and cause cell oxidative stress. Red Guava Fruitghurt is a probiotic with the basic ingredients of fruit. The purpose of this study is to determine the effect of fruitghurt on the MDA levels in the blood of mice induced by CCl4. This was an experimental study conducted using mice (Mus musculus) which were divided into three treatment groups -- the negative control group (that were not induced and were not given fruitghurt), the positive control group (that were induced with CCl4 but not given fruitghurt), and the treatment group (that were induced with CCl4 and given fruitghurt). The data obtained in the study were analyzed using the one-way ANOVA test, and the results showed significant differences between the treatment groups. Giving fruitghurt to the P1 group had a significant effect on reducing malondialdehyde levels compared to the positive control group, with a P-value of 0.03. Lactobacillus acidophilus bacteria found in this fruitghurt can reduce oxidative stress by inhibiting ascorbic acid oxidation, reducing activity and capturing radial superoxide anions, hydrogen peroxide, and free radicals, and inhibiting lipid oxidation. So this fruitghurt has antioxidant properties. This is also strengthened by the content of red guava which is rich in phenolics, flavonoids, and vitamin C that have good free radical scavenging activity, so guava fruit is a natural source of antioxidants. [ABSTRACT FROM AUTHOR]

The study aims to assess the larvicidal efficacy of Diospyros kaki fruit extracts (crude and solvents) against the rice field mosquito, Culex vishnui, which is the vector of Japanese encephalitis. Under simulated semi-field conditions, the larvicidal property of the most effective extract was tested against third instar Cx. vishnui larvae. The crude extract of fruits has a strong larvicidal effect. The larvicidal bioassay with chloroform: methanol extractive outperformed the other solvent extractives of fruits. After 72 h of exposure in a larvicidal bioassay, chloroform: methanol extractive had the lowest LC50 and LC90 values, which were 46 ppm and 186 ppm, respectively, against 1st instar larvae. The semi-field results showed that chloroform: methanol extract was likewise highly effective against third instar Cx. vishnui larvae, with the maximum mortality rate of 100% at 400 ppm concentration after 72 h of exposure. Three major bioactive compounds were identified namely 2,7-dibutoxy-fluoren-9-one- oxime, Pthalic acid, and Tris (2,4-ditert-butylphenyl) phosphate. Non-target organisms, such as Diplonychus annulatum nymphs, were found to be unaffected by the said extracts. Thus, we can claim that the fruits of D. kaki exhibit outstanding larvicidal efficacy against Cx. vishnui mosquito. [ABSTRACT FROM AUTHOR]

Recently, the researchers are focused on the biotherapeutic properties of medicinal as well as edible plants. Inflammation, fever, and pain are common symptoms associated with various diseases, necessitating effective therapeutic potentials for relief and healing. This study aimed to assess the therapeutic impact and mechanisms of action of the methanolic fruit extract of Carica papaya for anti-inflammatory, antipyretic, and analgesic activities via in-vivo and in-vitro approaches. For inducing inflammation, pain, and fever in albino rats, carrageenan, acetic acid dilution in distilled water, and yeast dilution in saline were used. The four different concentrations (50, 100, 200, and 400 mg/kg) of methanolic extract of C. papaya fruit were used to prevent inflammation, pain, and fever. Diclofenac and paracetamol were used as standard drugs in this study. The methanolic extract of C. papaya fruit showed efficient antipyretic and anti-inflammatory inhibition (90 and 80%, respectively), but less efficient analgesic inhibition (36%). Similarly, the in-silico study used fruit bioactive compounds such as quercetin as ligand molecules, and proteins for anti-inflammatory and antipyretic activities were 1bp4 and 4x37, respectively. The docking process was done using ligand and protein molecules. The results of the in-silico study were the same as those of the in-vivo study; anti-inflammatory and antipyretic activity binding energy values were more efficient than those of an analgesic. In conclusion, the methanolic extract of C. papaya fruit in in-silico and in-vivo studies proved less efficient against pain and more efficient against inflammation and fever. [ABSTRACT FROM AUTHOR]

Food waste is an excellent source of various bioactive secondary metabolites. In addition, food waste is a global issue, and more research is being focused on the conversion of value-adding products from food waste, especially fruits. In this study, bioactive polyphenols were extracted from orange peels. The parameters that affect the extraction of polyphenols from orange peels were optimized using response surface methodology. Ethanol was used to extract bioactive polyphenols, and the extraction time (min), extraction temperature, and ethanol concentration (%) were analyzed. Orange peels are an excellent source of polyphenols, and the yield was increased under optimized extraction conditions (p<0.05). The polyphenol content increased twofold (38.4 mg GAE/g) under the optimized extraction conditions (53% ethanol, 51 degrees centigrade and 96 min extraction time). Crude polyphenols exhibited significant antibacterial activity against Bacillus subtilis (19±1 mm zone of inhibition), followed by Staphylococcus aureus (17±2 mm zone of inhibition) (p<0.05). In addition, orange peel extract exhibited antifungal activity against A. flavus and C. albicans. The ethanol extract exhibited DPPH activity (1.61±0.03 mg GAE/100 g) and ABTS reducing power (1.48±0.01 mg GAE/100 g). The polyphenol compounds exhibited antibiofilm activity against biofilm-producing Bacillus subtilis and increased in a dose-dependent manner (p<0.05). [ABSTRACT FROM AUTHOR]

Anthraquinones exhibit a significant group of natural and synthetic quinone derivatives because of their biological activities and industrial applications. Rhamnaceae is one of the families known to contain different kinds of anthraquinones. In this study, it was aimed to quantify rhein, emodin, chrysophanol and physcion in fruits of Rhamnus petiolaris Boiss. & Balansa belonging to Rhamnaceae by solid phase extraction and high performance liquid chromatography with ultraviolet detection. The anthraquinones were separated using a C18 analytical column. Gradient elution was performed using a mobile phase consisted of 0.1% o -phosphoric acid solution and methanol. Analytes were detected at 254 nm. Calibration curves were prepared in the range of 0.25–5.00 μg/mL for rhein, chrysophanol, physcion, 1.00–50.00 μg/mL for emodin. Limits of detection and quantification were between 0.07–0.11 and 0.20–0.34 μg/mL, respectively. Relative standard deviations were ≤ 5.78% in repeatability and intermediate precision studies. Accuracy was determined as relative mean error (8.17–12.06%). Extraction was achieved by maceration with acetone and ethanol, followed by hydrophilic–lipophilic balance solid phase extraction. Recoveries were between 96.2 and 109.6%. The developed and validated method was successfully performed to quantify rhein, emodin, chrysophanol and physcion in R. petiolaris fruit extracts. Only physcion was not detected above limit of detection. [ABSTRACT FROM AUTHOR]

Mycotoxins are a major threat to animal and human health, as well as to the global feed supply chain. Among them, aflatoxins, fumonisins, zearalenone, T-2 toxins, deoxynivalenol, and Alternaria toxins are the most common mycotoxins found in animal feed, with genotoxic, cytotoxic, carcinogenic, and mutagenic effects that concern the animal industry. The chronic negative effects of mycotoxins on animal health and production and the negative economic impact on the livestock industry make it crucial to develop and implement solutions to mitigate mycotoxins. In this review, we summarize the current knowledge of the mycotoxicosis effect in livestock animals as a result of their contaminated diet. In addition, we discuss the potential of five promising phytogenics (curcumin, silymarin, grape pomace, olive pomace, and orange peel extracts) with demonstrated positive effects on animal performance and health, to present them as potential anti-mycotoxin solutions. We describe the composition and the main promising characteristics of these bioactive compounds that can exert beneficial effects on animal health and performance, and how these phytogenic feed additives can help to alleviate mycotoxins' deleterious effects. [ABSTRACT FROM AUTHOR]

Purpose: This study aims to find out how pomposia fruit powder (Syzygium cumini L.) mixed with roasted coffee (RCO) affected antioxidants, phytochemicals, lipid peroxidation inhibition and sensory attributes. Design/methodology/approach: Pomposia fruits (Syzygium cumini L.) powder (PFP) was integrated with RCO at levels of 0.0%, 5.0%, 10.0%, 15.0% and 20.0%. RCO, PFP and binary mixtures containing RCO: PFP were evaluated for their total phenolics (TP), total flavonoids (TF), anthocyanin content (AC), antioxidant activity and phenolic compounds fractionation. The oxidative indices of coffee oil samples were measured throughout different storage intervals. Additionally, sensory characteristics of RCO enriched with different PFP doses were evaluated. Findings: PFP samples exhibited the greatest TP content (1910 mg/100 g), whereas RCO samples had the lowest concentration (1090 mg/100 g). As more PFP was added, the corresponding coffee blends' concentrations of TP, TF and AC were improved significantly. PFP possesses a radical-scavenging activity that is about 1.20 times more than RCO's. DPPH (2,2-diphenyl-1-picrylhydrazyl) radical-scavenging activity jumped significantly from 74.13% in control (untreated) samples to 77.64%, 78.39% and 80.15% for samples enriched with 10.0%, 15.0% and 20% PFP, respectively. Significant increases in gallic acid, hesperidine, benzoic acid, chlorogenic acid, hispertin, catechol, quercetin, pyrogallol and rutin were detected when RCO was mixed with different quantities of PFP. At the end of storage trial, the coffee oil treated with 20% PFP had AV, PV and TBA values that were about 1.70, 1.95 and 1.66 times lower, respectively, than those of the control sample that had not had PFP addition. The RCO with 5.0% PFP achieved the greatest over acceptability grades. Originality/value: To the best of the authors' knowledge, this study was the first study to evaluate the effect of incorporating various level of pomposia fruit powder into RCO. The findings shows that adding different concentrations of pomposia fruit powder into RCO can indeed enhance the radical-scavenging activity of the coffee and potentially extend its shelf life. [ABSTRACT FROM AUTHOR]

In this study, the antioxidant capacity, the effect on anti-acetylcholinesterase and anti-urease enzyme inhibition, and kidney stone-reducing effects of different extracts from M.myrtifolia. In addition, the phytochemical content of the bioactive methanol extract was analyzed by HPLC-DAD. The methanol extract showed strong DPPH (IC50:0.036 mg/mL) radical scavenging, Cu (II) ion (6.535 mmTroloxE) and Fe3+ (6.476 FeSO4) ion reducing antioxidant activity compared to other extracts. The methanol extract contained the highest amount of phenolic (400 mgGAE) and flavonoid (2.519 QuE) compounds. Moreover, the methanol extract had significant anticholinesterase (78.935%), anti-urease (71.014%) and calcium oxalate anti-crystallization (18.22%, 37.427%) activities. The findings show that all extracts exhibited varying amounts of antibacterial activity against Staphylococcus aureus. The bioactive methanol extract contained rosmarinic acid (31.35 µg), quercetin (12.99 µg) and chlorogenic acid (3.55 µg). The analyzed compounds are thought to contribute significantly to the pharmacological effect of the methanol extract. [ABSTRACT FROM AUTHOR]

This study investigated the effects of microencapsulating Myrtus communis fruit extracts within intact and plasmolysed Saccharomyces cerevisiae yeast cells on the phenolic compounds and antioxidant capacity of the extracts. Spectroscopic techniques were employed to quantify polyphenols, flavonoids, and tannins, while UPLC-ESI-MS-MS analysis was utilized to identify and quantify phenolic compounds before and after microencapsulation. Molecular docking was conducted to assess the interaction between yeast cell components and the major identified compounds. Microencapsulation was found to result in a diminished content of polyphenols, flavonoids, and tannins. Phenolic compounds showed higher polyphenol content when encapsulated in plasmolysed cells compared to non-plasmolysed yeast cell. Three major compounds were detected in the microcapsules: myricetin, chlorogenic acid, and coumaric acid, with myricetin exhibiting the highest level of interaction with target proteins. FTIR analysis revealed the interaction between phenolic compounds and yeast cell components, while SEM showed aggregation of microcapsules within plasmolysed microcapsules. Encapsulation of myrtle extract in yeast cells preserved cell wall integrity and microcapsule morphology, with superior thermal stability of encapsulated extracts compared to their non-encapsulated counterparts as demonstrated by TGA–DSC analysis. An enhancement in antioxidant activity after encapsulation was also observed, with the highest activity in plasmolysed yeast cell microcapsules. [ABSTRACT FROM AUTHOR]

The purpose of this study was to compare the efficacy of citrus peel phenolic compounds extracted using carboxylated multi -walled carbon nanotubes (MWCNT –COOH) with common synthetic antioxidants [butylhydroxyanisole (BHA) and butylhydroxytoluene (BHT)] in stabilizing sunflower oil (SO) during frying (180 ± 5 °C, 24 h). To evaluate the antioxidant activity of these compounds, total phenolic, and flavonoid contents, reducing power, ABTS scavenging activity, β -carotene bleaching ability, and 1, 1 -diphenyl - 2 - picrylhydrazyl (DPPH) radical scavenging activity were measured. Extracts were combined at various concentrations (100 -1000 ppm) with SO, and both synthetic antioxidants were standardized at 200 ppm. Thermal -oxidative stability was investigated by assessing the change in peroxide value (PV), color, free fatty acid (FFA) content, conjugated diene value (CDV), and thiobarbituric acid (TBA). Bitter orange peel extract (BPE) displayed a higher content of phenolic compounds and antioxidant activity. At 1000 ppm, it showed a lower FFA content, PV, TBA, and CDV. It can be used as an alternative to synthetic antioxidants. As a result, citrus peel phenolic compounds extracted with MWCNT –COOH can be used as preservatives in frying oils. [ABSTRACT FROM AUTHOR]

In this study, the essential oil composition, antioxidant activities, phenolic and flavonoid contents, and of metanolic extract of Iranian and Afghanestanian populations of Ferula assa -foetida were evaluated. Essential oils were analyzed by using GC and GC/MS, The antioxidant activity were measured by 2, 2 -diphenyl - 1 -picrylhydrazyl and β - carotene/linoleic acid assay, also phenol and flavonoid content were measured by gallic acid and quercetin as standard compound. Result showed that propenyl sec butyl disulfide (42.72%), acetone, dimethyl mercaptole (14.84%) and gamma - eudesmol (8.6%) were the main compouned found in the Iranian population while; the Afghanestanian population were rich of propenyl sec butyl disulfide (39.61%), acetone, dimethyl mercaptole (18.25%) and 8 -ethyl -pentathiadecane (18.97%). In DPPH and β -carotene/linoleic acid tests, Iranian and Afghanestanian populations have stronger antioxidant activity respectively, also, the results demonstrated that the Afghanestanian population have more phenolic and flavonoid contents than the Iranian population. Overall, the results showed that the two populations investigated in this research have differences in terms of the type and percentage of essential oil compounds, antioxidant activity and phenol and flavonoid content. These differences are probably caused by differences in ecological factors such as the habitats of these populations and the influence of these factors on the studied parameters. [ABSTRACT FROM AUTHOR]

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The present study was performed to evaluate the therapeutic impact of Diospyros kaki fruit aqueous extract (DKFAE) on ethanol induced peptic ulcer. The phytochemical studies of DKFAE were investigated using colorometric analysis. Gastric ulcer was induced by one dose of ethanol (5 ml/Kg, b.w) on 24 h empty stomach. Then, the plant extract (200, 400 mg/kg) was orally administrated for 2 weeks. Famotidine (FAM: 40 mg/kg, b.w.): a reference drug was also tested. The effect of mixture dose between the fruit extract and FAM (DKFAE, 50 mg/kg PC, p.o. + FAM, 50 mg/kg PC, p.o.) was also evaluated. One hour after induction of ulcer blood samples were collected, stomach acidity and volume, as well as lesion counts were measured, then stomach and intestine of scarified rats were subjected to biochemical, macroscopic and microscopic studies. Results showed that DKFAE exhibited an important antioxidant potential. In vivo, the results showed that alcohol induced gastric damage, improving oxidative stress markers level such as MDA and H2O2, gastric and intestinal calcium and free iron. The intoxication by ethanol also produce an inflammation occurred by high level of the C-reactive protein (CRP) and alkaline phosphatase (ALP) activity in plasma. In contrast, DKFAE and the mixture dose significantly protect against macroscopic and histological injuries, the secretory profile disturbances, lipid peroxidation, antioxidant enzymes activities and non enzymatic antioxidant level decrease induced by ethanol administration. More impressively, the mixture dose exerted the more excellent effect than DKFAE and famotidine each alone showing is possible synergism. [ABSTRACT FROM AUTHOR]

Background: Peptic ulcer disease (PUD) is a prevalent health concern. Although proton pump inhibitors (PPIs), H2 receptor blockers, prostaglandin analogs, etc., are available as anti-ulcer agents, they have their own limitations. The clinical assessment of these medications revealed adverse effects, relapse frequency, and tolerance development, raising doubts about their effectiveness. Thus, there is a need to search for newer synthetic and herbal formulations that have fewer side effects and improved efficacy. Emblica officinalis (EO) or amla has shown to have antioxidant and antisecretory properties; hence, it was chosen for the study. Aims and Objectives: (1) The aim of this study was to screen the anti-ulcer activity of EO aqueous fruit extract in non-steroidal anti-inflammatory drug-induced peptic ulcer models of albino rats and (2) to compare the anti-ulcer effect of EO aqueous fruit extract with the standard drug omeprazole. Materials and Methods: Four groups comprising six albino rats each were formed. Group 1 (the control) received distilled water, followed by indomethacin 25 mg/kg orally. Group 2 (standard) received omeprazole 10 mg/kg, followed by indomethacin 25 mg/kg. Groups 3 and 4 received the aqueous extract of EO in 250 mg/kg and 500 mg/kg doses, respectively, followed by indomethacin at 25 mg/kg. After 1 h of indomethacin administration, the stomach was examined for ulceration. The severity score was determined to calculate the ulcer index (UI) and percentage inhibition. The results were analyzed statistically using the Mann--Whitney U-test (SPSS software version 22). Results: In the screening model mentioned above, the test agent at dosages of 250 mg/kg and 500 mg/kg body weight resulted in a significant decrease in total severity score, UI, and percentage inhibition as compared to the control group. The comparison of the results of the standard drug omeprazole with those of the test groups was statistically insignificant. Conclusion: The gastroprotective activity of amla (EO) may be due to its antioxidant and antisecretory properties. This study proves the gastroprotective action of EO. [ABSTRACT FROM AUTHOR]

This study aimed to assess the synergistic antioxidant and anticancer effects of methanolic extracts derived from Rubus ellipticus and Boerhavia diffusa fruits against the HeLa cell line. The methanolic extracts were prepared from the fruits of R. ellipticus and B. diffusa, and their antioxidant potential was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging activity assay and the ferric reducing antioxidant power (FRAP) assay. The anticancer effects of benzoic acid and rutin extracted from the aforementioned fruits were also investigated against the HeLa cell line using the 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide assay to measure the cell metabolic activity. Using Synergy Finder plus software, the bioactive compounds were tested to explore any synergistic effects. R. ellipticus exhibited higher antioxidant potential as revealed by higher DPPH scavenging activity and FRAP value compared with B. diffusa. The benzoic acid extracted from R. ellipticus demonstrated potent anticancer activity against the HeLa cell line, with an IC50 of 1.07 µg/mL. Similarly, rutin extracted from B. diffusa displayed moderate anticancer activity with an IC50 of 1.4 µg/mL while exhibiting minimal impact on normal cell lines. The combination studies of the extracted bioactive compounds revealed a synergistic effect. These findings suggest the therapeutic potential of R. ellipticus and B. diffusa in combating the oxidative stress and cancer. Their bioactive compounds like benzoic acid and rutin were observed to act synergistically. Further investigations are warranted to elucidate the underlying mechanisms and evaluate their applicability in clinical settings. [ABSTRACT FROM AUTHOR]

Chronic exposure to ultraviolet (UV) radiation can cause degenerative changes in the cells, fibrous tissues and blood vessels. Moreover, microbiological attacks pose severe risks to human health and are a major cause of sickness worldwide. The aim of this work is to develop UV protective and antimicrobial materials using zinc oxide (ZnO) colloidal solution with carrot and orange peel extract. Treated samples were subjected to color strength (K/S value) measurement, scanning electron microscope (SEM) image analysis, attenuated total reflectance (ATR) Fourier transform infrared (FTIR) spectroscopy analysis, ultraviolet protection factor (UPF) test, bacterial reduction rate analysis and bursting strength measurement. The study revealed that the treated fabric exhibited remarkable UV protection capabilities, as evidenced by low transmission percentages across UV wavelengths and an outstanding UPF rating of 50, signifying excellent defense against harmful ultraviolet radiation. Additionally, antibacterial tests revealed the significant effectiveness of the treatment against common bacterial strains, with impressive reduction rates for Staphylococcus aureus (99.99%) and Escherichia coli (99.17%). Furthermore, the fabric's bursting strength, both in dry and wet conditions, remained nearly unchanged after treatment. ATR‐FTIR analysis and SEM imaging provided insights into the phytochemical compounds that give protection against UV composition (44.63–49.91) and surface morphology of the treated fabric, elucidating the mechanisms behind its enhanced properties. Due to the availability of carrot and orange peel in nature and low cost of raw materials, this process can be considered to apply commercially for further research purposes. Highlights: Utilization of carrot and orange peel waste.Successful application of zinc oxide for antibacterial and ultraviolet protective materials.Excellent defense against harmful ultraviolet radiation.Significant effectiveness against common bacterial strains.Low cost and commercially viable functional materials. [ABSTRACT FROM AUTHOR]

Cranberry fruit extracts have been shown to inhibit expression of pro-inflammatory cytokines in THP-1 cells and reduce colonic tumor burden and tissue inflammation in a mouse model of colitis. These activities are attributed to both the triterpenoid and polyphenol constituents of the fruit. The pentacyclic triterpenoids ursolic acid (UA), oleanolic acid (OA), corosolic acid (CA), maslinic acid (MA), and esters of UA and OA occur in the waxy layer of cranberry peel, and their content in cranberry products is likely to vary with the fruit source and processing methods. UPLC-MS (ultra performance liquid chromatography-mass spectrometry) was applied to determine the four triterpenoid acids and their esters in cranberry products and raw materials. Cranberry pomace, a side stream in juice production, was a rich source at 64,090 µg total triterpenoids/g DW. Cranberry juice beverages ranged from 0.018 to 0.26 µg/g of product, fruit samples ranged from 6542 to 17,070 µg/g DW, and whole berry products contained up to 2665 µg/g DW. Free UA was the most plentiful triterpenoid in all samples. These analyses illustrate the potential value of an underutilized side stream in cranberry juice production and highlight potential benefits of whole fruit products. [ABSTRACT FROM AUTHOR]

Piper peepuloides a well-known medicinal plant of Piperaceae family is used by many tribal groups to treat inflammation and diabetics in India. In the study, different extracts such as petroleum ether, chloroform, ethyl acetate, ethanol and aqueous of Piper peepuloides fruits are evaluated for its secondary metabolites, in vitro antioxidant and Anti-bacterial activity. Among various extracts, Piper peepuloides fruits ethanol extracts showed the maximum amount of phenolics (1394.64 mg GAE/g extract), tannin (1365.21 mg GAE/g extract) and flavonoid (797.89 mg RE/g extract) content. It also revealed the presence of highest antioxidant property by estimating DPPH% (IC50: 55.28 µg/mL), ABTS (32055.56 µM TE/g extract), FRAP (248.33), Phosphomolybdenum (196.13 mg AAE/g), Superoxide (65.6%) radical scavenging activity and Piper peepuloides ethyl acetate and ethanol extract of fruit sample showed a significant zone of inhibition for Citrobacter freundii (14 mm), Enterobacter cloacae (13 mm), Burkholderia cepacian (16 mm), Klebsiella pneumonia (14 mm) and Escherichia coli (17 mm) at a concentration of 10 mg/ml. Piper peepuloides fruit extracts have a tremendous amount of antioxidant potential, making them a good source of natural antioxidant supplements for food to protect against oxidative stress-related diseases. [ABSTRACT FROM AUTHOR]

Bioactive flavonoid epicatechin has been reported in the peel of litchi fruit but isolated from its hydroalcoholic extracts. This study isolated epicatechin with cellular glucose uptake modulatory and ROS production inhibitory properties from the ethyl acetate (EtOAc) extract using a bioassay-guided approach. The fruit peel was defatted with hexane and sequentially extracted using dichloromethane (DCM), EtOAc, methanol (MeOH) and water. In vitro phytochemical models, namely antioxidant (Fe3+ reducing, radical scavenging and anti-linoleic acid peroxidative) and glycaemic control (α-glucosidase and α-amylase inhibitory and glucose uptake modulatory), were employed for the bioassay-guided isolation, while the isolated compound was characterised using NMR and mass spectrometry and assessed for dose-dependent inhibition of α-glucosidase and lipopolysaccharide (LPS)-induced cellular ROS production, as well as modulation of cellular glucose uptake. Relative to the other extracts, the EtOAc extract had appreciable phenol and flavonoid contents, which perhaps influenced its potent anti-lipid peroxidative (65.0%) and α-glucosidase inhibitory (52.4%) effects. The α-glucosidase inhibitory potency of the fractions (1–8) from the EtOAc extracts correlated with their flavonoid contents, with fraction 5 outperforming other fractions. The fraction comprised a pool of fractions obtained from the DCM:MeOH:water (7:3:0.281 v/v/v) solvent system. LC-MS revealed the predominant presence of epicatechin in fraction 5, which was later isolated from one of the sub-fractions (sub-fraction 4) of fraction 5. This sub-fraction had stronger anti-lipid peroxidative (65.5%), α-glucosidase inhibitory (65.8%) and glucose uptake modulatory (38.2%) effects than the other sub-fractions from fraction 5, which could have been influenced by the isolated epicatechin. Moreover, the isolated epicatechin inhibited α-glucosidase (IC50 = 35.3 µM), modulated cellular glucose uptake (EC50 = 78.5 µM) and inhibited LPS-induced ROS production in RAW 264.7 macrophages in a dose-dependent fashion [IC50 = 18.9 µM; statistically comparable (p > 0.05) to ascorbic acid, IC50 = 9.57 µM]. Epicatechin from litchi peel EtOAc extract could potentiate glucose uptake modulatory, α-glucosidase inhibitory and ROS suppressive capacities, which could be influential in the use of litchi fruit peel for managing diabetes and associated oxidative damage. [ABSTRACT FROM AUTHOR]

The editorial "Achievements in Applications of Antioxidants and Bioactive Compounds in Food: From Agriculture to Health Benefits" discusses the increasing popularity of natural foods and additives due to health concerns, emphasizing the health-promoting qualities of bioactive compounds found in various plants and superfoods. The focus is on utilizing waste from the agrifood sector to reduce waste, conserve resources, and create value-added products. Studies explore the antioxidant properties and processing potential of medicinal herbs and underutilized bioresources for developing products in various sectors, such as nutraceuticals, cosmetics, and functional foods. The issue also addresses the development of new active antioxidant packaging systems to protect food and bioactive ingredients. [Extracted from the article]

Consumers are increasingly concerned about cosmetic ingredients' origin, looking more than ever to sustainable and greener formulations. The Natural Park of Montesinho, located in Portugal, is characterized by an enormous fauna and flora diversity. Among them, beeswax and strawberry trees (Arbutus unedo) have attracted the cosmetic researchers' interest due to their bioactive compounds' richness, particularly fatty acids and phenolic compounds. The main goal of this study was to develop an innovative cosmetic product with antioxidant properties composed by both matrices. Briefly, samples were obtained in the Natural Park of Montesinho in October 2022. Beeswax was analysed for lipid profile and contaminants, while extracts were obtained from fruits by ultrasound-assisted extraction (UAE) using water as a solvent. The effect of extraction time (15–90 min) was studied on the total phenolic content (TPC), in vitro antioxidant/antiradical activity, and reactive oxygen species (ROS) scavenging capacity. The beeswax lipid profile presented a high incidence of palmitic, oleic, and linoleic acids. The extract obtained at 60 min presented the highest TPC (30.27 mg GAE/g dw) and antioxidant/antiradical activities (ABTS = 30.36 mg AAE/g dw; DPPH = 43.83 mg TE/g dw; FRAP = 415.61 µmol FSE/g dw). An IC50 of 19.78 µg/mL was achieved for the hypochlorous acid, while for superoxide radical and peroxyl radical the IC50 were, respectively, 90.51 µg/mL and 0.19 µmol TE/mg dw. The phytochemical profile revealed a high content of gallic acid, and catechin and its derivatives. The hydrophilic cream developed revealed ideal technological parameters, particularly its stability. [ABSTRACT FROM AUTHOR]

Orange (Citrus sinensis L.) is a common fruit crop widely distributed worldwide with the peel of its fruits representing about 50% of fruit mass. In the current study, orange peel was employed to mediate the synthesis of silver nanoparticles (AgNPs) in a low-cost green approach. Aqueous extracts of suitably-processed peel were prepared using different extraction methods; and their phytochemical profile was identified. Based on phytochemical screening, amount of main phytochemicals, free radical-scavenging ability, reducing power and antioxidant activity, the peel extract prepared by boiling seemed to be the most promising. Thus, major compounds of this extract were identified by gas chromatography-mass spectrometry. Potency of the peel extract to mediate the synthesis of AgNPs was then monitored by visual observation, UV-visible spectroscopy, energy dispersive X-ray analysis, transmission electron microscopy and zetametry. Color change of the reaction mixture to brown and absorption peak at 450 nm indicated AgNPs formation. Characterization of AgNPs revealed spherical shape, size of 30–40 nm, zeta potential of -18.2 mV and yield conversion of 82%. The as-synthesized AgNPs had antioxidant capacity (free radical-scavenging ability, reducing power and antioxidant activity) lower than that of the orange peel extract. However, these biogenic AgNPs had antitumor activity (IC50 of 16 ppm against HCT-116 and 1.6 ppm against HepG2 cell lines) much higher than the peel extract that was completely non-toxic to the considered cell lines. [ABSTRACT FROM AUTHOR]

: Accurate detection of immature green citrus fruits in trees is one of the most critical steps for production decisions, such as early yield prediction, precise water and fertilizer management, and regulation of number of fruits hanging. However, the large model has been confined to identifying the immature citrus, due to the similar green citrus to the canopy background. Great challenges have been brought to rapidly and accurately detect the fruits, even to deploy the model. In this study, an improved model, YOLO-GC (You Only Look Once-Green Citrus) was proposed to detect the green citrus fruits using YOLOv5s. The improved model was also deployed into the edge mobile devices, in order to achieve the real-time and convenient detection of green citrus fruits in trees. Firstly, the original backbone network was replaced with a lightweight GhostNet one, because the YOLOv5s network model was large and difficult to deploy. Meanwhile, the attention was then improved to the green citrus features. Accuracy degradation was reduced after the model was lightweight. Global Attention Mechanism (GAM) was embedded in the backbone network and the feature fusion layer, in order to extract the fruit features in complex environments. Secondly, a BiFPN (Bi-directional Feature Pyramid Network) architecture was introduced into the feature fusion layer for the multi-scale weighted feature fusion, in order to improve the dense and small-targeted fruits. Finally, the GIoU (Generalized Intersection over Union) loss function combined with Soft-NMS (Soft-Non-Maximum Suppression) was used to optimize the bounding box regression, in order to reduce the omission caused by the occlusion and overlapping of fruits and branches. The experimental results showed that the weight model memory of YOLO-GC was reduced by 53.9%, compared with the YOLOv5s. The number of parameters and the average inference time were reduced by 55.2% and 46.2%, respectively, whereas, the average precision (AP0.5) was improved by 1.2 percentage points. There was a lower amount of fruit leakage and misdetection in a variety of complex natural environments. The comprehensive performance of the YOLO-GC model was superior to that of CenterNet and seven commonly used network models, such as the YOLOv5s, YOLOv7, YOLOX, YOLOv8, CenterNet, Faster R-CNN, and RetinaNet target networks. The average accuracy of the YOLO-GC model was improved by 1.2, 1.3, 1.4, 0.9, 1.7, 4.6, and 3.9 percentage points, respectively, only 6.69 MB of weighted memory, thus achieving 97.6%, 90.3%, 97.8%, and 97.0% for the precision, recall, average precision, and F1 score, respectively. The YOLOGC model was then deployed to the Android mobile App for testing. The detection accuracy reached 97.2%, which was 2.4 percentage points higher than that of the YOLOv5s. Furthermore, the inference duration (103ms) was reduced by 85.8%. The IC model fully met the requirements of high-accuracy recognition and real-time inference of green citrus on the Android phone side. The finding can provide technical support to detect the green-like fruits in complex environments. The improved model was also deployed in the edge intelligent devices. [ABSTRACT FROM AUTHOR]

The increasing demand for natural compounds as an alternative to synthetic antioxidants and conservans has led to the utilization of secondary plant metabolites in the food industry, as these bioactive compounds possess great antioxidative and antimicrobial properties without side effects on human health. Despite this, the sensitivity of plant-derived compounds is a restrictive factor in terms of their full potential. The current research aimed to characterize rosehip-fruit-extract-loaded liposomes (non-treated and UV-irradiated) in terms of their density, surface tension, viscosity, chemical composition (FTIR and HPLC analyses), and thermal behavior. In the storage stability study, the vesicle size, polydispersity index (PDI), zeta potential, conductivity, and mobility of the liposomes were monitored. FTIR analysis confirmed that the plant compounds were successfully loaded within the carrier, while no chemical reaction between the rosehip fruit extract and phospholipids was detected. The results of the HPLC analysis evidence the high potential for liposomal encapsulation to protect sensitive bioactives in the rosehip fruit extract from the degrading effect of UV irradiation. The size of the rosehip-fruit-extract-encapsulated liposomes increased on the seventh day of storage from 250 nm to 300 nm, while the zeta potential values were between −21 mV and −30 mV in the same period and further stabilized over 60 days of monitoring. In Vitro release studies in water and simulated gastrointestinal fluids showed that the presence of enzymes and bile salts (in intestinal fluid) enhanced the rosehip–polyphenol permeability from liposomes (70.3% after 6 h) compared with their release in water after 24 h and in gastric fluid after 4 h (38.9% and 41.4%, respectively). The obtained results indicate that the proliposome method was an effective method for rosehip fruit extract liposomal encapsulation and for the delivery of these plant-derived bioactives in foods. [ABSTRACT FROM AUTHOR]

Discarded unripe kiwifruits (DUKs) are regarded as the major agro-byproducts in the production of kiwifruits, which have abundantly valuable secondary metabolites. Nevertheless, owing to the limited knowledge about the differences in phytochemicals and bioactivity between DUKs and mature kiwifruits, the utilization of DUKs in the food industry remains scarce. Hence, to promote their food applications, the phenolic compounds and bioactivity of discarded unripe, mature, and overripe fruits from three red-fleshed kiwifruit cultivars were studied and compared. The results revealed that the levels of total phenolics, total flavonoids, and total procyanidins in kiwifruits varied significantly by maturity stage. In addition, our findings demonstrated that DUKs possessed much higher contents of valuable phenolic compounds (e.g., chlorogenic acid (CHA), neochlorogenic acid (NCHA), gallic acid (GA), protocatechuic acid (PA), procyanidin B1 (ProcB1), procyanidin B2 (ProcB2), procyanidin C1 (ProcC1), quercetin 3-O-glucoside (QueG), and quercetin 3-O-rhamnoside (QueR)) than mature and overripe kiwifruits. Furthermore, DUKs exerted much stronger in vitro antioxidant capacity, inhibitory effects on α-glucosidase, and anti-inflammatory activity than mature and overripe kiwifruits, which were mainly attributed to their higher contents of total polyphenols and individual phenolic components, such as GA, CHA, NCHA, PA, ProcB1, ProcB2, ProcC1, and QueR. Overall, these findings provide sufficient evidence for the development and utilization of DUKs in the food/functional food industry. [ABSTRACT FROM AUTHOR]

The current investigation focuses on producing zinc oxide nanoparticles (ZnO NPs) utilizing a quick, economical, and environmentally friendly approach employing aqueous fruit extracts of Dysoxylum binectariferum plant. The ZnO NPs were thoroughly characterized using P‐XRD, FTIR, SEM, EDS, and BET techniques. The synthesized nanoparticles' anti‐bacterial properties were evaluated against Gram‐negative bacteria (E. coli and P. aeruginosa) and Gram‐positive bacteria (S. aureus and S. pyogenes). The findings indicated that the synthesized material exhibited greater efficacy against Gram‐negative bacterial infections, especially E. coli. Furthermore, the anti‐fungal activity was examined using the C. albicans strain, demonstrating that the synthesized ZnO NPs were twice as effective against C. albicans compared to the conventional medication Griseofulvin. In contrast, Griseofulvin, with a 500 μM MIC against the C. albicans pathogenic fungal strain, did not display the same level of efficacy. [ABSTRACT FROM AUTHOR]

Phyllanthus emblica L. fruit extract (PFE) was introduced to improve ZVI/H2O2 technology, and the efficiency and mechanism of PFE promoting ZVI/H2O2 technology were explored. With the introduction of PFE, the Norfloxacin (NOR) removal rate and kobs of the process were improved by 41.17% and 5.08 times, respectively. In the ZVI/H2O2/PFE process, the degradation of NOR by the attack of ROS is the main pathway for decontamination and is dominated by the heterogeneous reaction on the catalyst surface. PFE contains 13.92 g/L titratable acid and has good complexing ability and antioxidant ability. The mechanism of PFE promoting ZVI/H2O2 technology was based on lowering the pH, complemented by chelation and antioxidant capacity. With the introduction of PFE, the utilization rate of the reagent was significantly increased (7.56 times for ZVI and 3.21 times for H2O2), the applicable pH range was widened (6–9) and the iron sludge was reduced (32.80%). Meanwhile, the concept of UPR is proposed for the first time. The result is the key role to the selection of green promoters in the ZVI/H2O2 process depends on the abundance of titratable acid, followed by a certain chelating ability and antioxidant capacity. [ABSTRACT FROM AUTHOR]

Background Methods Results Conclusions Sensitive skin is a highly prevalent problem. The objective of the study was to assess whether the tested products are effective and safe in terms of improving the symptoms of sensitive skin.A clinical randomized split‐face study was carried out on 24 healthy female subjects. Three cosmetic combinations were tested versus vehicle: product A (Solía Thermal Spring Water–TSW–from Cantabria, Spain + diatom algae–P. tricornutum–extract), product B (Solía TSW + diatom algae extract + Annona cherimola Fruit Extract) and product C (Solía TSW + diatom algae extract + Annona cherimola Fruit Extract + niacinamide). Prior to each application of the study Product (A, B, or C)/vehicle, 10% of aqueous solution of capsaicin to induce skin irritation was applied, mimicking the symptoms of sensitive skin. Stinging and burning sensations were evaluated at different time points.All three tested products A, B, and C showed to act better in calming the symptoms induced by capsaicin when compared to the vehicle.The tested products would be an interesting option for treating stinging and burning sensations in sensitive skin patients. [ABSTRACT FROM AUTHOR]

Dengue infection has become one of the most important mosquito-borne diseases worldwide and is caused by the dengue virus (DENV). Recently, neither patent drug, phytopharmaceutical medicine, nor standardized herbal medicine has been officially available against DENV. Dried red jujube fruit (Ziziphus jujuba) ethanol extract has been proven to have an antiviral effect, anti-inflammatory efficacy, and antioxidant properties, which have potential activity against DENV infection. This research was conducted to analyze the antiviral activity of dried red jujube fruit ethanol extract against DENV-2 in vitro. The halfmaximal cytotoxic concentration (CC50) and half-maximal inhibitory concentration (IC50) were examined on Vero cells by a 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, whereas the halfmaximal effective concentration (EC50) was determined using luminescence assay. The selectivity index (SI) value was determined from the ratio of CC50 and EC50. Dried red jujube fruit ethanol extracts inhibited DENV-2 in 24.59%, 22.39%, 17.98%, 14.5%, 6.42%, and 1.28% at 80 μg/mL, 40 μg/mL, 20 μg/mL, 10 μg/ mL, 5 μg/mL, and 2.5 μg/mL, respectively. The extract exhibited antiviral activity against DENV-2, showing a CC50 of 67.73 μg/mL, an IC50 value of 166.18 μg/mL, and an EC50 of 64.87 μg/mL, with an SI of 1.04. The LD50 value was 707.95 mg/kg. Dried red jujube fruit ethanol extract could be a potential candidate for developing an antiviral against DENV-2. [ABSTRACT FROM AUTHOR]