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Start Over Author "Gu, Ming" Publication Type Academic Journals
1,486 results on '"Gu, Ming"'

31. Identification and validation of biomarkers in membranous nephropathy and pan-cancer analysis

Author

Yue Yang, Gu-ming Zou, Xian-sen Wei, Zheng Zhang, Li Zhuo, Qian-qian Xu, and Wen-ge Li

Subjects
membranous nephropathy, pan-cancer, differential expression gene, potential link, biomarkers, Immunologic diseases. Allergy, RC581-607
Abstract

BackgroundMembranous nephropathy (MN) is an autoimmune disease and represents the most prevalent type of renal pathology in adult patients afflicted with nephrotic syndrome. Despite substantial evidence suggesting a possible link between MN and cancer, the precise underlying mechanisms remain elusive.MethodsIn this study, we acquired and integrated two MN datasets (comprising a single-cell dataset and a bulk RNA-seq dataset) from the Gene Expression Omnibus database for differential expression gene (DEG) analysis, hub genes were obtained by LASSO and random forest algorithms, the diagnostic ability of hub genes was assessed using ROC curves, and the degree of immune cell infiltration was evaluated using the ssGSEA function. Concurrently, we gathered pan-cancer-related genes from the TCGA and GTEx databases, to analyze the expression, mutation status, drug sensitivity and prognosis of hub genes in pan-cancer.ResultsWe conducted intersections between the set of 318 senescence-related genes and the 366 DEGs, resulting in the identification of 13 senescence-related DEGs. Afterwards, we meticulously analyzed these genes using the LASSO and random forest algorithms, which ultimately led to the discovery of six hub genes through intersection (PIK3R1, CCND1, TERF2IP, SLC25A4, CAPN2, and TXN). ROC curves suggest that these hub genes have good recognition of MN. After performing correlation analysis, examining immune infiltration, and conducting a comprehensive pan-cancer investigation, we validated these six hub genes through immunohistochemical analysis using human renal biopsy tissues. The pan-cancer analysis notably accentuates the robust association between these hub genes and the prognoses of individuals afflicted by diverse cancer types, further underscoring the importance of mutations within these hub genes across various cancers.ConclusionThis evidence indicates that these genes could potentially play a pivotal role as a critical link connecting MN and cancer. As a result, they may hold promise as valuable targets for intervention in cases of both MN and cancer.

Published
2024
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32. Unraveling DDIT4 in the VDR-mTOR pathway: a novel target for drug discovery in diabetic kidney disease

Author

Hai-tao Lu, Yuan-yuan Jiao, Tian-yu Yu, Jing-xuan Shi, Jing-wei Tian, Gu-ming Zou, Lin Liu, and Li Zhuo

Subjects
diabetic kidney disease (DKD), DNA damage inducing transcription factor 4 (DDIT4), vitamin D receptor, mammalian target of rapamycin (mTOR) signal pathway, autophagy, oxidative stress, Therapeutics. Pharmacology, RM1-950
Abstract

Introduction:Diabetic kidney disease (DKD) necessitates innovative therapeutic strategies. This study delves into the role of DNA damage-inducing transcription factor 4 (DDIT4) within the VDR-mTOR pathway, aiming to identify a novel target for DKD drug discovery.Methods:Transcriptome data from the Gene Expression Omnibus Database were analyzed to assess the expression of mTOR and VDR expression in human renal tissues. Clinical samples from DKD patients and minimal change disease (MCD) controls were examined, and a DKD animal model using 20-week-old db/db mice was established. DDIT4 plasmid transfection was employed to modulate the VDR-mTOR pathway, with its components evaluated using immunohistochemistry, real-time quantitative PCR (qRT-PCR), Western blotting, and enzyme-linked immunosorbent assay (ELISA).Results:Changes in the expression of the VDR-mTOR pathway were observed in both DKD patients and the animal model. Overexpression of DDIT4 increased VDR expression and decreased levels of mTOR, p70s6k, and 4E-BP1. Furthermore, DDIT4 treatment regulated autophagy by upregulating LC3I expression and downregulating LC3II expression. Notably, DDIT4 alleviated oxidative stress by reducing the levels of lipid peroxidation product MDA, while simultaneously increasing the levels of superoxide dismutase (SOD) and glutathione (GSH), underscoring the role of DDIT4 in the pathological process of DKD and its potential as a therapeutic target.Conclusion:Unraveling DDIT4’s involvement in the VDR-mTOR pathway provides insights for innovative DKD drug discovery, emphasizing its potential as a therapeutic target for future interventions.

Published
2024
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45. SAPT: a synchrotron-based proton therapy facility in Shanghai

Author

Zhang, Man-Zhou, Li, De-Ming, Shen, Li-Ren, Zhang, Hai-Rong, Chen, Zhi-Ling, Du, Han-Wen, Gu, Ming, Li, Rui, Liu, De-Kang, Pu, Yue-Hu, Yu, Jun-Feng, Chen, Jian-Feng, Chen, Chu, Guo, Chun-Long, Guo, Hao, Jiang, Ge-Yang, Jiang, Zhi-Qiang, Jin, Lin, Li, Wen-Jing, Li, Xiu-Fang, Lin, Ye, Liu, Ming, Liu, Yong-Hao, Liu, Ya-Juan, Lv, Ming, Mi, Qing-Ru, Ouyang, Lian-Hua, Shi, Wei-Guo, Shu, Hang, Tang, Qi-Sheng, Wang, Kun, Wang, Zhi-Shan, Wu, Jun, Wu, Xiao-Bing, Xu, Jia-Qiang, Xu, Wen-Zhen, Yin, Chong-Xian, Yu, Cheng, Yuan, Ren-Xian, Yuan, Qi-Bing, Zhang, Hai-Qun, Zhang, Miao, Zhang, Wen-Zhi, Zhao, Li-Ying, Zhou, Wei-Min, Fang, Shou-Xian, Sun, Xi-Dong, and Zhao, Zhen-Tang

Published
2023
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