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Start Over Author "Hu, Xiaoya" Publication Type Academic Journals
513 results on '"Hu, Xiaoya"'

17. Efficient label-free chemiluminescent immunosensor based on dual functional cupric oxide nanorods as peroxidase mimics

Author

Li, Juan, Cao, Yue, Hinman, Samuel S, McKeating, Kristy S, Guan, Yiwen, Hu, Xiaoya, Cheng, Quan, and Yang, Zhanjun

Subjects
Analytical Chemistry, Chemical Sciences, Prevention, Antibodies, Immobilized, Biomimetic Materials, Biomimetics, Biosensing Techniques, Carcinoembryonic Antigen, Copper, Humans, Immunoassay, Limit of Detection, Luminescent Measurements, Nanotubes, Peroxidase, Dual-functionalization, Cupric oxide nanorods, Inununosensor, Peroxidase mimetics, Chemiluminescence, Carcinoembryonic antigen, Immunosensor, Biomedical Engineering, Nanotechnology, Bioinformatics, Analytical chemistry, Biomedical engineering
Abstract

Dual-functional cupric oxide nanorods (CuONRs) as peroxidase mimics are proposed for the development of a flow-through, label-free chemiluminescent (CL) immunosensor. Forming the basis of this cost-efficient, label-free immunoassay, CuONRs, synthesized using a simple hydrothermal method, were deposited onto epoxy-activated standard glass slides, followed by immobilization of biotinylated capture antibodies through a streptavidin bridge. The CuONRs possess excellent catalytic activity, along with high stability as a solid support. Antigens could then be introduced to the sensing system, forming large immunocomplexes that prevent CL substrate access to the surface, thereby reducing the CL signal in a concentration dependent fashion. Using carcinoembryonic antigen (CEA) as a model analyte, the proposed label-free immunosensor was able to rapidly determine CEA with a wide linear range of 0.1-60ngmL-1 and a low detection limit of 0.05ngmL-1. This nanozyme-based immunosensor is simple, sensitive, cost-efficient, and has the potential to be a very promising platform for fast and efficient biosensing applications.

Published
2018

41. Amphiphilic Polymer Capped Perovskite Compositing with Nano Zr‐MOF for Nanozyme‐Involved Biomimetic Cascade Catalysis.

Author

Ye, Qiuyu, Yuan, Enxian, Shen, Jin, Ye, Mingli, Xu, Qin, Hu, Xiaoya, Shu, Yun, and Pang, Huan

Subjects
PEROVSKITE, CATALYSIS, OPTICAL materials, POLYMERS, PRUSSIAN blue, VITAMIN C, ZIRCONIUM compounds, PEROXIDASE
Abstract

CsPbX3 perovskite nanocrystal (NC) is considered as an excellent optical material and is widely applied in optoelectronics. However, its poor water stability impedes its study in enzyme‐like activity, and further inhibits its application in biomimetic cascade catalysis. Herein, for the first time, the oxidase‐like and ascorbate oxidase‐like activities of an amphiphilic polymer capped CsPbX3 are demonstrated, and its catalytic mechanism is further explored. Furthermore, an all‐nanozyme cascade system (multifunctional CsPbBr3@Zr‐metal organic framework (Zr‐MOF) and Prussian blue as oxidase‐like and peroxidase‐like nanozyme) is constructed with a portable paper‐based device for realizing the dual‐mode (ratiometric fluorescence and colorimetric) detection of ascorbic acid in a point‐of‐care (POC) fashion. This is the first report on the utilization of all‐inorganic CsPbX3 perovskite NC in biomimetic cascade catalysis, which opens a new avenue for POC clinical disease diagnosis. [ABSTRACT FROM AUTHOR]

Published
2023
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42. A bimetallic metal–organic framework with high enzyme-mimicking activity for an integrated electrochemical immunoassay of carcinoembryonic antigen.

Author

Shu, Yun, Yan, Lu, Ye, Mingli, Chen, Long, Xu, Qin, and Hu, Xiaoya

Subjects
CARCINOEMBRYONIC antigen, METAL-organic frameworks, POLYETHYLENE terephthalate manufacturing, IMMUNOASSAY, CONDUCTIVE ink, CATALYTIC activity, POLYETHYLENE
Abstract

Metal–organic frameworks (MOFs) show excellent catalytic activity and have been widely applied in diagnosis of diseases and tumors. However, current assay methods usually involve cumbersome configurations and complicated procedures, which inhibit their practical applications. Hence, a Cu–Ni MOF/carbon printed electrode (CPE)-based integrated electrochemical immunosensor was constructed for highly sensitive and efficient determination of carcinoembryonic antigen (CEA). First, highly conductive carbon ink was screen-printed onto a polyethylene terephthalate substrate to manufacture a CPE. Afterward, an aminated Cu-Ni MOF was prepared by a typical solvothermal strategy and modified on the CPE. Owing to its excellent peroxidase activity, the Cu–Ni MOF can catalytically oxidize hydroquinone using hydrogen peroxide, which greatly amplifies the peak current signal. Then the formation of an immune complex inhibited the catalytic activity of the MOF, thus enabling the quantitative determination of CEA content with a wide linear range of 0.5 pg mL−1–500 ng mL−1 and a low detection limit of 0.16 pg mL−1. Furthermore, the Cu–Ni MOF/CPE-based integrated portable electrochemical immunosensor also showed satisfactory performance in the detection of CEA in clinical serum samples with excellent accuracy, showing great potential for application in point-of-care disease diagnosis. [ABSTRACT FROM AUTHOR]

Published
2023
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