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Your search keyword '"John J Desmarais"' showing total 2 results
Start Over Author "John J Desmarais" Publication Type Academic Journals
2 results on '"John J Desmarais"'

1. Author Correction: CasX enzymes comprise a distinct family of RNA-guided genome editors.

Author

Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, and Doudna JA

Abstract

In this Article, owing to issues with the first 30 nucleotides of the sgRNA, which run in the opposite direction, corrections have been made to the Protein Data Bank (PDB) accessions in the 'Data availability' section, and this also affects Figs. 3, 4, Extended Data Fig. 6, Supplementary Table 1 and Supplementary Video 1. The original Article has been corrected online. See the accompanying Amendment for further details.

Published
2019
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2. CasX enzymes comprise a distinct family of RNA-guided genome editors.

Author

Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brötzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, and Doudna JA

Subjects
CRISPR-Associated Proteins chemistry, CRISPR-Associated Proteins metabolism, Cryoelectron Microscopy, DNA chemistry, DNA metabolism, DNA ultrastructure, DNA Cleavage, Escherichia coli genetics, Evolution, Molecular, Gene Silencing, Genome, Bacterial genetics, Genome, Human genetics, Humans, Models, Molecular, Nucleic Acid Conformation, Protein Domains, RNA, Guide, CRISPR-Cas Systems metabolism, CRISPR-Associated Proteins classification, CRISPR-Associated Proteins ultrastructure, CRISPR-Cas Systems genetics, Gene Editing
Abstract

The RNA-guided CRISPR-associated (Cas) proteins Cas9 and Cas12a provide adaptive immunity against invading nucleic acids, and function as powerful tools for genome editing in a wide range of organisms. Here we reveal the underlying mechanisms of a third, fundamentally distinct RNA-guided genome-editing platform named CRISPR-CasX, which uses unique structures for programmable double-stranded DNA binding and cleavage. Biochemical and in vivo data demonstrate that CasX is active for Escherichia coli and human genome modification. Eight cryo-electron microscopy structures of CasX in different states of assembly with its guide RNA and double-stranded DNA substrates reveal an extensive RNA scaffold and a domain required for DNA unwinding. These data demonstrate how CasX activity arose through convergent evolution to establish an enzyme family that is functionally separate from both Cas9 and Cas12a.

Published
2019
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