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Nowadays, it is almost unknown how fetoplacental insufficiency of mothers affects the formation of the reproductive system in male offspring during puberty. Therefore, the aim of this work was to study the histological structure of the testes of male offspring born to mothers of different ages with fetoplacental insufficiency and to evaluate the effectiveness of a new pharmaceutical composition for the correction of pathological conditions of pregnancy. The experiment involved healthy mature female Wistar rats in young (3–4 months) and mature (8–10 months) reproductive age. Eight groups with 7 pregnant females in each one were formed: groups 1 and 2 — intact animals of young and mature reproductive age, respectively; groups 3 and 4 — females with experimental fetoplacental insufficiency of young and mature reproductive age; groups 5 and 6 — young and mature animals with experimental fetoplacental insufficiency and addition to food depending on the weight of animals from 11 to 19 days of pregnancy pharmaceutical composition. Groups 7 and 8 — young and mature animals with experimental fetoplacental insufficiency with addition a comparison drug Dipyridamole to the food. Modeling of fetoplacental insufficiency was performed by daily subcutaneous injection to females from the 12th to the 18th day of pregnancy 50% oil solution of carbon tetrachloride at a dose of 2 ml/kg body weight. Offspring were decontaminated on the 50th day of life (puberty) by rapid decapitation. Samples of male testicles were fixed in 10% formalin solution, performed on alcohols of increasing strength, poured into paraffin. Sections were made from the blocks, which were stained with hematoxylin and eosin. Qualitative assessment of histostructure was performed on sections of the body in rats. Summing up the results of histological analysis and morphometric parameters characterizing the condition of the testicles of rats, we can draw the following conclusions. In the offspring born to reproductively young females with fetoplacental insufficiency, inhibition of the rate of germ cell differentiation was found, while in the offspring born to reproductively mature females with fetoplacental insufficiency, the negative effects of this effect were leveled and the rate of spermatogenesis increased. When the pharmaceutical composition was administered to both young and mature pregnant females on the background of fetoplacental insufficiency, it activated the processes of proliferation and differentiation of spermatogenic epithelium in their offspring, which was reflected in the appearance of mature sperm in the seminal tubules. The comparison drug Dipyridamole, administered according to the same regimen to both young and mature pregnant females with fetoplacental insufficiency, also helped the 50-day-old offspring of these females to restore the rate of spermatogenesis at the level of the pharmaceutical composition.

Introduction. Local therapy is an important method for treatment of dermatitis used to suppress the skin inflammation and the main related symptoms: hyperemia, edema, pruritus, lichenification etc. However today it is important to pay more attention to elimination of the skin dryness, restoration of the damaged epithelium and improvement of the skin barrier functions in dermatitis therapy. Considering all the requirements mentioned above, cream and ointment that correspond to the modern requirements of the local therapy were developed. They promote the decrease of inflammatory processes in skin (Mometasone furoate and Methylprednisolone atseponat are strong GCS with antipruritic, anti-inflammatory, vasoconstrictive, antiproliferative action and with minimum side effects), eliminate excessive skin dryness, restore the damaged epithelium, improve the skin condition and normalize the barrier functions of the skin (ceramides are the natural ceramides of the human’s skin). The purpose of this work was morphological study of the rats’ skin during treatment with cream “Mometasone with ceramides” and ointment “Methyl­prednisolone with ceramides” in the conditions of contact dermatitis inducted by turpentine. Materials and methods. Skin of animals was an object of the research after its treatment with cream “Mometasone with ceramides” and ointment “Methylprednisolone with ceramides”. The comparator preparations were cream “Elocom” and ointment “Advantan”. In the experiment 36 rats divided into groups were used. All the preparations were applied on the skin in a thin layer once a day. After the 5th day of the treatment animals were taken out of the experiment and all the tissue materials were fixed in 10% solution of formalin for morphological studies carrying out. Sections were stained with hematoxylin and eosine. An examination was carried out under the microscope Micros 400. Results of the research. The results showed that the therapeutic applications of the investigated preparations help to eliminate manifestations of nonspecific dermatitis which was registered in the majority of rats with the control pathology. The therapeutic effect of the developed cream “Mometasone with ceramides” and ointment “Methylprednisolone with ceramides” exceeded such of the comparator preparations cream “Elocom” and ointment “Advantan”. Thus, ointment “Methylprednisolone with ceramides” practically completely prevents development of dermatitis manifestations: in 100% of rats leads to the normal or approximately normal morphological structure of the skin; cream “Mometasone with ceramides” eliminates the signs of dermatitis in the majority of the animals, 83,3% of rats had normal skin and only 16,7% had insignificant ulcerations. Under the action of ointment “Advantan” 66% of rats had normal skin, 33,3% had small ulcer disturbances of epidermis. under the action of cream “Elocom” 33,3% of rats had normal skin, 33,3% had extensive ulcer defects, in 33,3% weak inflammatory reaction occurred. Conclusions. During experiment it was established that cream “Mometasone with ceramides” and ointment “Methylprednisolone with ceramides” have anti-inflammatory action (interfere dermatitis development) and restore normal morpho­logical structure of the skin. Adding ceramides to the drugs with GCS is expedient as they promote restoring of the damaged epithelium with normal differentiation of the layers of the skin structure (cornification processes are not affected, the thickness of the epidermal layer is close to normal, it has clear differentiation of the layers, the state of collagen fibers in derma is within normal range). Therefore further pharmaceutical study of GCS drugs with ceramides for the purpose of creating an effective and safe preparation for treatment of dermatitis is perspective.