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Recent interest in scaling commercial kelp industries in Western cultures is juxtaposed by the apparent challenges in achieving more consistent and predictable yields. As such, site-level factors are a dominant and recurring conversation amongst stakeholders. The availability of seawater nitrogen (nitrate, ammonium, total N) can be highly variable across space and time and is often one of the top concerns for site selection and permitting. This study questions the relative importance of nitrogen availability on the yield of Saccharina latissima (sugar kelp) across five commercial farms on the U.S. East and West Coasts over two seasons, highlighting the relative influence of other interacting factors (i.e., farm design). We hypothesized that nitrate would strongly correlate with the harvested yield. Our results show significant spatial and annual variability in the kelp yield and ambient nutrients across and within farms, but with weak covariance. Standard linear regression suggests that seawater nitrogen is a poor explanatory factor for kelp yield, explaining 11.0% of the variation around the mean compared to the line spacing (explaining 26.1%) and the interaction between the total N and the line spacing (explaining 50.0%). Quartile regression, however, suggests that total N alone, is the strongest predictor of a lower threshold in terms of the yield (0.10 quartile, r2 = 0.431) relative to the median (0.50 quartile, r2 0.081). As such, seawater nitrogen may be a more useful metric in predicting baseline kelp yields rather than realized yields, and production above that baseline is likely more dependent on other factors that may or may not interact with seawater nitrogen. [ABSTRACT FROM AUTHOR]

Aeromonas salmonicida is an important pathogen that causes furunculosis in trout and salmon with high morbidity and mortality, resulting in significant economic losses in aquaculture. Overuse of antibiotics has led to the continuous emergence of drug-resistant strains. Hence, there is an urgent need to find an alternative environmentally friendly antimicrobial agent. In this study, we isolated a virulent phage of A. salmonicida, named ASG01, which belongs to the Myoviridae family and maintains lytic activity at a pH value range from 4 to 12 and in the temperature range from 30 °C to 60 °C. The whole genomic sequence of ASG01 showed 82% similarity to Aeromonas phage pAh6-C. The cell wall hydrolase (Cwh)-encoding gene from the genome of ASG01 was predicted and heterologously expressed. Notably, in the absence of additional phage genes, endogenous expression of Cwh could lyse E. coli cells and greatly inhibit the growth of tested fish pathogenic bacteria. The lytic activity of Cwh was eliminated when the predicted active site was mutated. These results indicate that Cwh of ASG01 possessed excellent lytic activity and a wide antibacterial spectrum, suggesting its potential as an effective enzybiotic. [ABSTRACT FROM AUTHOR]

Wheat (Triticum aestivum L.) production is necessary for global food and nutrition security. Reducing plant height is an effective strategy to increase lodging resistance, harvest index, and eventually wheat yield. Rht8 is a gibberellic acid–sensitive reduced height (Rht) gene and has been cloned. Rht8 encodes a ribonuclease H‐like (RNHL) superfamily protein that reduces plant height without reduction in coleoptile length. High‐throughput diagnostic markers are not available for precise selection of RNHL in wheat breeding. In this study, we found that different cultivars may carry 1–3 copies of RNHLs on chromosome 2D, and only 2D carries the semi‐dwarf allele of RNHL although the wild‐type RNHL allele is detected in several other chromosomes. We developed two kompetitive allele specific PCR (KASP) markers, Rht8‐KASP‐V2 and Rht8‐KASP‐V3, based on the causal variation in RNHL. The two markers co‐segregated with plant height in a recombinant inbred population and were validated in a panel of hard winter wheat breeding lines from the 2022 Regional Germplasm Observation Nursery and Regional Performance Nursery. Rht8 has not been extensively deployed in US hard winter wheat and development of the diagnostic KASP markers will facilitate Rht8 transfer to US wheat cultivars. Core Ideas: Each cultivar carries 1–3 copies of ribonuclease H‐like domains (RNHLs) on 2D and mutations in all those copies determine Rht8 phenotype.Two kompetitive allele‐specific PCR (KASP) markers were developed for the recently cloned Rht8 gene.The two markers were diagnostic for Rht8 in a wheat diversity panel.Low Rht8 frequency in US wheat suggests high potential of using Rht8 in the United States to improve US wheat production. [ABSTRACT FROM AUTHOR]

The single‐molecule electronic devices offer the ultimate solution to miniaturize integrated circuits, and subthreshold swing (SS) is the key indicator of the power consumption for single‐molecule transistors but is still quite restricted. In this study, the redox‐mediated single‐molecule transistor with a SS down to 120 mV decade−1 in the faradaic potential region via the electrochemical‐STM break junction (EC‐STM‐BJ) technique is fabricated. With an off‐state leakage current of less than 10 pA and an on/off ratio of 100, the balance between low static power consumption and a high switching ratio is achieved. The theoretical investigations reveal that the conductance tuning is from the difference of transmission in the redox process. The study provides a new strategy for the design of single‐molecule transistors with promising SS, which is essential for the potential application of single‐molecule devices. [ABSTRACT FROM AUTHOR]

The single‐molecule electronic devices offer the ultimate solution to miniaturize integrated circuits, and subthreshold swing (SS) is the key indicator of the power consumption for single‐molecule transistors but is still quite restricted. In this study, the redox‐mediated single‐molecule transistor with a SS down to 120 mV decade−1 in the faradaic potential region via the electrochemical‐STM break junction (EC‐STM‐BJ) technique is fabricated. With an off‐state leakage current of less than 10 pA and an on/off ratio of 100, the balance between low static power consumption and a high switching ratio is achieved. The theoretical investigations reveal that the conductance tuning is from the difference of transmission in the redox process. The study provides a new strategy for the design of single‐molecule transistors with promising SS, which is essential for the potential application of single‐molecule devices. [ABSTRACT FROM AUTHOR]

Wheat (Triticum aestivum L.) wild relatives are rich genetic resources for genetic improvement of wheat. The short arm of rye (Secale cereale L.) chromosome 1 (1RS) contains many beneficial genes for resistance to drought, insects, and diseases, therefore the 1RS wheat translocations have been widely used in wheat breeding programs worldwide. To facilitate marker‐assisted identification of 1RS in wheat breeding, we designed a set of Kompetitive allele‐specific polymerase chain reaction (KASP) markers to detect the translocations of 1RS with long arm from wheat chromosome 1A (1RS.1AL) and 1RS with long arm from wheat chromosome 1B (1RS.1BL) based on the sequence flanking single nucleotide polymorphisms (SNPs) generated by genotyping‐by‐sequencing (GBS) between wheat and rye panels. We identified a set of three SNP markers for diagnosis and differentiation of the two 1RS translocations in wheat. Among the three KASP markers, 1AS‐05056 is 1RS specific, and clearly separated the genotypes with 1RS (1RS.1AL and 1RS.1BL) from those without the two 1RS translocations; whereas 1AS‐29875 clearly identified the 1RS.1AL translocation and 1BS‐16654 separated 1RS.1BL from 1RS.1AL. The three new KASP markers together accurately identified the presence of 1RS translocation on either 1A or 1B chromosome in a panel of 95 representative U.S. winter wheat accessions, which were further validated in two additional U.S. hard winter wheat panels. Therefore, they can be used for routine screening of 1RS.1BL and 1RS.1AL translocation lines in wheat breeding programs. Core Ideas: 1RS, 1AS, and 1BS specific SNPs were identified by analyzing GBS‐SNPs from both wheat and rye panels.Three chromosome‐arm‐specific KASPs clearly detected the 1RS.1AL and 1RS.1BL translocations in wheat.Three KASP markers were diagnostic for 1RS.1AL and 1RS.1BL in two U.S. hard winter wheat panels. [ABSTRACT FROM AUTHOR]

Using glucosamine hydrochloride as the starting material, 4‐(methoxycarbonyl)‐5‐methyl‐1‐propyl‐1H‐pyrrole‐2‐carboxylic acid (3) was initially created as an intermediate compound by cyclization and oxidation in order to create novel high temperature stable pyrrole compounds. Furthermore, Steglich esterification was used to produce N‐substituted pyrrole esters (4 a–4 t). All the new compounds were verified using nuclear magnetic resonance (1H and 13CNMR), infrared spectroscopy (IR), and high resolution mass spectrometry (HRMS). Thermogravimetry (TG), differential scanning calorimetry (DSC), and pyrolysis‐gas chromatography/mass spectrometry (Py‐GC/MS) techniques were used to examine the thermal behaviors of the compounds 4 f, 4 g, 4 i, and 4 q. Ester bonds from the pyrolysis products 4 g, 4 i, and 4 q might be severed which were consisted of the raw materials as well as the substrates, such as menthol, phenethyl and pyrrole derivatives which are commonly utilized in the sector of spices in foods and cigarette. [ABSTRACT FROM AUTHOR]

In order to use single top flavors with roasted sweet aroma in heated tobacco products for flavor enhancement, puff-by-puff release of six roasted sweet aroma compounds in the aerosols of heated tobacco products was measured using GC-MS. The six aroma components were added into the tobacco rod at 0.01% and 0.05% of tobacco mass, and the puff-by-puff transfer rates were determined. The results showed that: 1) The puff-by-puff releases of the six aroma components in the aerosol of heated tobacco products increased first and then decreased with the puff number. 2) When added at 0.01% and 0.05%, their puff-by-puff transfer rates were between 0.5%-15.6% and 1.0%-9.6%, respectively, and the per puff transfer rate peaked in the third or fourth puff. 3) There was a good linear positive correlation between the total of puff-by-puff transfer rates of the six single flavors in aerosols and the puff number. 4) At the two addition rates, the total transfer rates of the six single flavors in aerosols were between 34.2%-73.1% and 23.3%-63.7% respectively. [ABSTRACT FROM AUTHOR]

Our team has initiated a selective breeding program for regional strains of sugar kelp, Saccharina latissima, to improve the competitiveness of kelp farming in the United States. Within our breeding program, we also include an endemic putative species, Saccharina angustissima, locally referred to as skinny kelp. We crossed uniclonal gametophyte cultures derived from 37 wild‐collected blades representing five sugar kelp strains and one skinny kelp strain to produce 104 unique crosses. Each cross was outplanted on a near‐shore research farm located in the Gulf of Maine (GOM). After the first farming season, our results indicated that sugar kelp and skinny kelp were interfertile, and produced mature and reproductively viable sporophytes. Morphological traits of individual blades varied depending on the parental contribution (sugar vs. skinny), with significant differences found in progeny blade length, width, thickness, and in stipe length and diameter. Despite these differences, wet weight and blade density per plot showed no statistical differences regardless of the cross. Given their published genetic similarity and their interfertility shown here, S. angustissima and S. latissima may not be different species, and may each contribute genetic diversity to breeding programs aimed at meeting ocean farming and market needs. [ABSTRACT FROM AUTHOR]

The interconnection of all things and industrial integration is the current trend of the times. Among them, the interconnection of all things is the demand of informationization of the times, and the industrial integration is the demand of industrial development. The interconnection of all things is realized based on wireless communication technology. It is necessary to combine the development of the tourist area and the surrounding culture. The relationship between tourist attractions and culture needs to be fully and effectively developed. In order to fully explore the advantages of the cooperation between the two, it is necessary to combine modern technology to package the tour process of each characteristic culture of the scenic spot. Virtual reality is a modern technology that can combine culture and tourism. Wireless communication and VR technology are applied to the development of integration of culture and tourism. The process of tourism will promote profound changes in the tourism model. Under the demand for informatization in various industries, the tourism industry is gradually developing in this direction. The integration of culture and tourism will also be driven by informatization and technology. This paper analyzes the current situation of culture and tourism, summarizes the problems existing in the current process of integration of culture and tourism, and finally puts forward targeted solutions. Mainly in the process of the integration and development of tourism and cultural industries, the traditional culture and scenic spots are the basic factors, combined with wireless communication and virtual reality technology, to develop a tourism industry with technological characteristics of the new era. [ABSTRACT FROM AUTHOR]

Urban social civilization and the quality of life of residents are gradually improved, and the development scale and trend of the leisure tourism industry have been growing. This paper constructs a multi-source data fusion model based on an ensemble learning algorithm, uses Ctrip 2020 open data set to train the model, and then obtains the tourism information data processing and prediction results. This paper takes the data of Ctrip as the training set and compares the trained model with the data of tunic and Feizhu. In this paper, sensor detection technology is used to analyze many famous scenic spots in China, including tourist type, gender, and location. The results show that tourism feature extraction results are consistent with data from trending flying bamboo, tunics, and other websites, according to the results of a multi-source fusion of tourism information. Among them, in the data of the first half of 2020, the prediction accuracy of the model after data processing is about 62%. Affected by the epidemic situation, the accuracy of the model is low. In the second half of the year, the prediction accuracy is 78%, which can be used to fuse tourism information in a short time. Therefore, the data show that the model has high learning ability and high trend prediction ability in tourism data processing, which can provide necessary information support for tourists. [ABSTRACT FROM AUTHOR]

Hessian fly (HF, Mayetiola destructor Say), is a destructive insect pest in most wheat (Triticum aestivum L.) growing areas worldwide. Growing resistant cultivars can effectively reduce HF damage. The wheat cultivar 'Seneca' was reported to carry resistance genes H7 on chromosome 5D and H8 with unknown location. To further validate the resistance gene locations in Seneca, a recombinant inbred line (RIL) population from Bobwhite × Seneca was genotyped using 3,330 single nucleotide polymorphism (SNP) markers generated from genotyping‐by‐sequencing (GBS) and phenotyped for HF resistance in the greenhouse experiments. Phenotypic analyses showed at least two genes conditioning HF resistance in Seneca, but none of them was mapped on chromosome 5D, as previously reported. Instead, one major gene, designated as H7, was mapped to chromosome 6A and explained 60.7–78.3% of the phenotypic variation. The other gene with a minor effect, designated as H8, was found on chromosome 2B and explained 3.2–4.7% of the phenotypic variation. Based on the physical locations of flanking markers for H7 in the Chinese Spring reference genome, H7 was physically located in a 6‐Mb interval on 6AL. This study reassigned the major gene H7 to chromosome 6A and the minor gene H8 to 2B in Seneca using a high‐density SNP map. Seventeen GBS SNPs mapped in the H7 region were converted into Kompetitive allele specific polymerase chain reaction (KASP) markers for selecting this gene in breeding. [ABSTRACT FROM AUTHOR]

In order to investigate the effects of cigarette paper properties on the combustion coal fallout propensity of burning slim cigarette, cigarette paper with different parameters ( grammage, air permeability, hemp pulp content, ash content, burn promoter content and proportion of potassium salts in burn promoter) was selected and the samples of fine cigarette were made. The parameters were set optimization. The results showed that the combustion coal fallout propensity of slim cigarettes decreased rapidly with the increase of paper grammage. The presence of air permeability, hemp pulp content and ash content in cigarette papers was able to promote the fallout propensity of combustion coal, in a content-dependent manner. Meanwhile, air permeability of cigarette paper exhibited a positive correlation to the combustion coal fallout propensity. The change of burn promoter content had no significant effect on reducing the tendency of the fallout propensity of combustion coal, while the excessively high proportion of potassium salts in burn promoter would cause the tendency of the fallout propensity of combustion coal to fall rapidly. The four parameters of air permeability, grammage, burn promoter content and proportion of potassium salts in burn promoter were sorted in descending order of the influence on the combustion coal fallout propensity as below: air permeability > grammage > proportion of potassium salts in burn promoter > burn promoter content. The combustion coal fallout propensity decreased from6. 3% to 2.5% with the optimum cigarette paper parameters of air permeability 50 CU, grammage 34 ⋅ m-2, proportion of potassium salts in burn promoter 50%, burn promoter content 1.5%. [ABSTRACT FROM AUTHOR]

Highlights • We modified the CO 2 transfer in social-economic system as a metabolic process in ecosystem. • We utilized ecological network analysis to study the flow variation in global, national, and bilateral scale. • We put up a plan to adjust current mitigation targets set by Paris Agreement based on our study results. Abstract A large share of total global CO 2 emissions results from the production processes of internationally traded goods and services. Such emissions have been referred to as virtual or embodied CO 2 flows. Hence, it is important to understand the directions, locations, and drivers of carbon flows resulting from global trade. To analyze these flows, hotspots, divisions of labor, and directions of flows in the global production and consumption web, we applied a multi-national framework based on a combination of multi-region input-output tables with ecological network analysis. We found that the CO 2 flows between the 40 key countries within the network doubled between 1996 and 2011, and the trade-related emission hub shifted from Europe to Asia. We analyzed a large number of CO 2 transfer paths and their changes over time. We found that the United States participated the most in big paths, including those that represent carbon flows between the United States and both developed and developing countries, whereas developing countries such as China and India participated more in the fast-growing paths, and especially in paths between pairs of developing countries. In contrast, traditionally active countries, including Russia and Germany, grew at a slower rate, resulting in a progressively smaller share in the global system. Taking these large and important CO 2 transfers into consideration, we proposed adjustments to the national mitigation targets set by the Paris Agreement. [ABSTRACT FROM AUTHOR]

CO 2 emission induced by international trade brings a plenty of pressure on global warming. Hence, it is important to understand multi-regional CO 2 flows that result from global trade and flow variation of different paths. After transforming capital flows into CO 2 carbon flows, we built an ecological network model of the global trade system's carbon metabolism. Through ecological network analysis, we identified the direct and indirect effects of temporal CO 2 flows in the network. We found that the total integral flow through the network experienced a remarkable increase during the study period. In addition, total import and export flows of four countries, including US, China, Germany and Russia, turned out to be rather big. Direct flow share was usually beyond 50% between two geographical adjacent countries, while that share was below 50% when two countries are far away. This research reveals the importance role of indirect flows and can therefore provide an empirical basis for adjusting and optimizing global trade network. [ABSTRACT FROM AUTHOR]

BACKGROUND Understanding the variance of antioxidant in wheat grain responses to irrigation and nitrogen (N) fertiliser management will improve the nutrient quality of wheat grain. Four N rates (0, 180, 240, and 300 kg ha−1) combined with irrigation times (I0, no irrigation; I1, jointing time irrigation; I2, jointing + flowering time irrigation), were used to determine the effect of N fertilisation and irrigation on total phenolic content (TPC), phenolic acid composition, and antioxidant activity (AOA) of wheat grain. RESULTS Irrigation, N fertilisation and their interactions had significant effect on TPC, total flavonoid content (TFC), AOA, p-coumaric acid (PCA), as well as vanillic acid (VA) and chlorogenic acid (CA). I1N300 treatment had the highest TPC at Zhengzhou and Wenxian (1451.5 µg g−1 and 1397.9 µg g−1, respectively) location, while I1N240 resulted in the highest TFC (0.75 mg g−1) and VA (19.77 µg g−1) at Wenxian. TPC, TFC, AOA, ferulic acid (FA), PCA and VA increased with N application rate (from 180 to 300 kg N ha−1). CONCLUSION An appropriate irrigation and N management improved antioxidant content and AOA in wheat grain. Generally, I1N240 and I1N300 treatment resulted in the higher TPC, TFC, AOA, as well as phenolic acid, i.e. FA and VA. © 2014 Society of Chemical Industry [ABSTRACT FROM AUTHOR]

With the increasing scale and scope of global trade, the magnitude of the CO 2 flows embodied in goods and services through international trade has aroused great concern among researchers and governments. In this study, we established a global network model of CO 2 transfers from 2001 to 2010 using ecological network analysis and data from the World Input-Output Database for 40 selected countries whose GDP accounted for more than 85% of the total global GDP. Based on the utility analysis, we determined the ecological relationships among the countries involved in the global trade network and their changes during the study period. The analysis revealed that competition and exploitation/control relationships dominated the global network, with each accounting for more than 40% of the total relationships throughout the study period; mutualism accounted for the smallest proportion (less than 4%). More than 80% of the competition and 75% of the exploitation/control relationships were within Europe or involved flows from Europe to North America or Asia. Finland, France, Japan, Greece, and Spain had the largest proportions of competition relationships. In Denmark, Luxembourg, Malta, and Switzerland, exploitation was dominant, whereas in Russia, Indonesia, and India, control was dominant. Our analysis identifies the key nodes of the many adverse ecological relationships within the global CO 2 network and those with more mutual relationships. Our work provides a scientific basis for developing more ecologically sustainable national and global CO 2 flows through trade. [ABSTRACT FROM AUTHOR]

Abstract: Flavonoids are the low molecular weight polyphenolic secondary metabolic compounds, and have various functions in growth, development, reproduction, and stress defense. However, little is known about the roles of the key enzymes in the flavonoids biosynthesis pathway in response to drought stress in winter wheat. Here, we investigated the expression pattern of flavonoids biosynthesis genes and accumulation of flavonoids in wheat leaves under drought stress. Quantitative real-time PCR analysis showed that there were a rapid increase in expression levels of TaCHS, TaCHI, TaF3H, TaFNS, TaFLS, TaDFR, and TaANS under drought stress in two wheat cultivars Aikang 58 (AK) and Chinese Spring (CS). The cultivar CS exhibited higher genes expression levels of TaCHS, TaCHI, TaF3H, TaFLS, TaDFR, and TaANS, and the cultivar AK showed a higher expression level of TaFNS gene during drought treatment. The increase rates of genes expression were superior in AK compared to CS. Total phenolics content, total flavonoids content, anthocyanin content, and schaftoside content in wheat leaves were enhanced during drought treatment and cultivar CS had a relative higher accumulation. These results suggest that the flavonoids pathway genes expression and accumulation of flavonoids compounds may be closely related to drought tolerant in wheat. Further, flavonoids response mechanism may be different between wheat cultivars. [Copyright &y& Elsevier]

• The hierarchical structure could give information to a low-carbon city. • Energy-related carbon emission of economic activities dominated the system. • Integrated flows indicated the importance of primary consumers and producers. • Beijing's spatial gradients transformed from single-center to multi-center. • Beijing's ecological hierarchy indicated a better organized metabolic structure. An integral analysis of urban carbon metabolism can support efforts to build a more sustainable city: the spatial gradients provide insights into how to guide future land use and cover change, while ecological network hierarchy provide insights into the self-mutualistic interactions of the city's carbon metabolic structure. In this study, we combined a spatially explicit network with carbon budget of energy consumption and land use and cover change in Beijing, China. From 1990 to 2010, energy related carbon emissions accounted for roughly 90% of the city's total. The network's integrated flux decreased at an average rate of 7.5%. The output flux primarily decreased when moving outward from the center through the periphery of southeastern plains and towards the northwestern mountains. In contrast, the input flux increased moving from the periphery to both the center and mountains. The ecological hierarchy was irregular based on both the driving and pull weights for the components of the model, with excessively small producer and decomposer layers and excessively large consumer layers. The changing structure of Beijing's carbon metabolism over time indicated a tendency to a better organized city. Understanding the evolution of these hierarchies during urban development provides a more solid empirical basis for improving use of the urban space and reducing carbon emission. [ABSTRACT FROM AUTHOR]

Ethnopharmacological Relevance: Alcoholic liver disease (ALD) is a growing public health concern caused by excessive alcohol consumption, but effective treatments are limited. Ge-Zhi-Jie-Jiu decoction (JJY) is a modified traditional Chinese herbal remedy that aims to alleviate ALD. This formula contains various components such as Ge Hua, Ge Gen, Zhi Ju Zi, and other medicinal-food herbs. However, the specific pharmacotherapeutic compounds of JJY and its pharmacological mechanisms remain unclear., Aim of the Study: This study aimed to elucidate the molecular mechanism and pharmacodynamic basis of JJY in treating ALD., Materials and Methods: UPLC-Q-Orbitrap HRMS, HPLC fingerprinting, and LC-MS techniques were used for the composition identification and quality control of JJY. The pharmacological components and molecular mechanisms of JJY in anti-ALD were then predicted using network pharmacology and molecular docking approaches. Ultimately, an acute alcoholic liver injury mouse model was developed, and the potential mechanisms were verified by hematoxylin-eosin (H&E), Oil Red O, and TUNEL staining, real-time fluorescence quantitative PCR (RT-qPCR), Western blot (WB) and molecular docking analysis., Results: The results showed that the main components of JJY are organic acids, flavonoids, and isoflavonoids, in which puerarin, daidzein, glycitein, ononin, quercetin, and tectorigenin can be used as the indicator components of JJY. In addition, JJY might ameliorate ALD through several pathways, including potentially promoting alcohol metabolism via alcohol-metabolizing enzymes, and possibly inhibiting oxidative stress, inflammation and apoptosis via the Nrf2/Keap1/HO-1 and MAPK signaling pathways. Furthermore, JJY may also alleviated hepatic lipid accumulation through the PPARα signaling pathway., Conclusions: JJY has significant anti-ALD efficacy with multiple mechanisms. This study offers a solid experimental foundation for JJY's development as a medicine with anti-ALD characteristics and elucidates its probable active components., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Objective: The purpose of this study was to evaluate the clinical diagnostic value of metagenomic next-generation sequencing (mNGS) for tuberculosis (TB)., Methods: This retrospective study included 52 patients with suspected TB infection. mNGS, targeted PCR, acid-fast staining and, T-SPOT.TB assay were performed on the specimen. The positive rate of mNGS and traditional detection methods was statistically analyzed. Pathological tests were performed when necessary., Results: In total, 52 patients with suspected of TB in this study were included in the analysis, and 31 patients were finally diagnosed with TB. Among 52 patients, 14 (26.9%) cases were positive for acid-fast staining. The positive rate of T-SPOT.TB assay in 52 patients was 73.1% (38/52). Among 52 patients, 39 (75%) were detected positive for Mycobacterium tuberculosis ( M TB) by mNGS. Regarding the detection rate of MTB, mNGS were as high as 75% (39/52), whereas acid-resistant staining was only 26.9% (14/52), which showed a statistically significant difference (p<0.05). The positive rates of T-SPOT.TB assay and mNGS were not statistically significant (p>0.05). Of the 52 suspected TB patients, 24 had targeted PCR, of which 18 were PCR positive. In 24 patients, the positive rate of PCR was 75%, and the positive rate of mNGS was 100%, with statistical difference between them (p<0.05)., Conclusions: The detection rate of MTB by mNGS was higher than that by conventional acid-fast staining and PCR, but not statistically significant compared with T-SPOT.TB assay. As an adjunctive diagnostic technology, mNGS can be combined with traditional detection methods to play a guiding role in the diagnosis and treatment of TB., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Liu, Wang, Li and Yu.)