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Magnetic flux leakage (MFL) signal prediction is important in ferromagnetic defect evaluation and reconstruction. At present, the magnetic dipole model (MDM) is the most widely applied method because of its high efficiency. However, it has low accuracy when applied to analysing complex defects (complex in profile and magnetic permeability) due to the original setting of uniform magnetic charge distribution. To solve the issue, this paper proposes an improved MDM method for MFL inspection of ferromagnetic materials by calculating the non-uniform magnetic charge distribution. It partly applies the finite volume method (FVM) to derive the magnetic charge distribution on the defect surface, which makes it able to take into account the defect's profile and magnetic permeability distribution. The new magnetic charge distribution can increase the MDM's accuracy for complex defect MFL field prediction with little extra cost. Experimental results show that, when compared with traditional MDM, the proposed one can achieve a maximum 69% decrease in root mean squared error when analysing complex defects. Compared with the numerical method, the computation time could reach a great reduction. [ABSTRACT FROM AUTHOR]

The transportation industry plays a key role in reducing urban emissions of air pollutants and energy consumption. The transition from traditional fossil fuel-based vehicles (TFFBVs) to new energy vehicles (NEVs) is critical to China's strategic goal of reaching peak carbon dioxide (CO2) emissions before 2030 and achieving carbon neutrality before 2060. On the basis of the environmental status and development of NEVs in Chongqing in 2020, we designed scenarios for replacing TFFBVs in Chongqing with NEVs according to targets such as the number of proposed NEVs in China's 14th Five-Year Plan. Following this, we evaluated the environmental and health benefits of NEVs and their monetary value using exposure–response and disease–cost methods. Replacing 18%, 35%, and 50% of TFFBVs with NEVs can create health benefits of approximately CNY 11.391 billion, CNY 21.696 billion, and CNY 30.443 billion, accounting for 4.56%, 8.68%, and 12.18%, respectively, of Chongqing's GDP in 2020. These amounts exceed the cost of government subsidies. Greater health benefits were derived from reducing the toxic emissions of nitrogen dioxide (NO2); the reduction in deaths caused by cardiovascular diseases created the best benefits for health endpoints, exceeding a 59% reduction in all three scenarios. Our study provides empirical support for promoting NEVs. [ABSTRACT FROM AUTHOR]

As high voltage pulse power capacitors, ceramic capacitors are widely used in high voltage pulse generators, trigger circuits, laser generators, and other fields. The capacitance of ceramic capacitors is closely related to the direct current (DC) bias voltage. However, the current capacitance measurement methods can only achieve a DC bias of 1 kV, which cannot meet the measurement requirements in high voltage environments. This paper proposes a capacitance measurement method that can accurately measure the capacitance under a DC bias of 3 kV. This method decouples the high DC bias voltage and high frequency alternating small signals and realizes low voltage calibration and high voltage isolation. The experimental results show that the proposed method measures the capacitance under a DC bias of 3 kV with a relative error within ±1%, which makes it possible to accurately quantify the capacitance hysteresis deviation in the process of increasing and decreasing back the voltage. [ABSTRACT FROM AUTHOR]

The parts of working machinery have always suffered from various working conditions in the process of use (e.g., high temperature, high humidity, and high pressure) that may generate flaws, pitting, thermal fatigue crack and other types of defects on the surface or inside. Because these tiny defects carry big safety risks, regular detection is necessary to ensure the reliability of the production equipment. [ABSTRACT FROM AUTHOR]

Introduction: Breast cancer metastasis suppressor 1 like (BRMS1-like)was first reported to be a component of the Sin3-HDAC complex, but the role in the progression of cancers was largely unknown. Our previous study reported that BRMS1L promoted the metastasis of breast cancer through facilitating the recruitment of HDAC complex to the promoter FZD10, and hence suppressing the transcription of FZD10. Methods: In this study, we detected the expression level of BRMS1L in esophageal squamous cell carcinoma (ESCC). The effect of BRMS1L in TE-1D (knockdown) and ECA-109 (overexpression) cell lines was explored by transwell assays, wound healing assays, and cell adhesion assays. Quantitative real‑time PCR, Western blot analysis, and luciferase assays were used to detect the interaction of the CBP/P300-BRMS1L-ITGA7 axis. Results: In the present study, we found that knockdown of BRMS1L promoted the migration, invasion, and epithelial–mesenchymal transition (EMT). Conversely, overexpression of BRMS1L inhibited the migration and invasion of ESCC. Mechanistically, BRMS1L exerted their metastasis-suppressing role via transcriptionally repress ITGA7 expression. Moreover, we revealed that CBP/p 300 regulated the expression of BRMS1L and might be responsible for the down-regulation of BRMS1L in ESCC. Conclusion: Collectively, we identified the role of CBP/p300-BRMS1L-ITGA7 axis in the metastasis of ESCC. [ABSTRACT FROM AUTHOR]

Panax Notoginseng Saponins (PNS) has been widely used in the prevention and treatment of cardiovascular and cerebrovascular diseases such as myocardial infarction, heart failure and cerebral infarction. However, oral administration of PNS showed low bioavailability because of its instability and poor membrane permeability in the gastrointestinal tract. Here, lipoprotein-inspired hybrid nanoparticles of PNS-Lecithin-Zein (PLZ-NPs) were prepared by using a simple phase separation method, which possessed a core-shell structure, where zein was used as protein part to replace the animal origin protein to increase the resistance to acid and enzymes while lecithin was used as the lipid composition to improve the oral absorption of PNS as well as to increase the drug loading capacity of PNS into the nanocarriers. The results of stability test showed that PLZ-NPs had robust enzymolysis resistance ability for acid and digestive enzymes of gastrointestinal environments. The fluorescent resonance energy transfer (FRET) assay confirmed the ability of LZ-NPs to be intactly absorbed by Caco-2 cell monolayer. Cell transport studies demonstrated that the permeability of PLZ-NPs in Caco-2/HT29-MTX co-culture cell model was 1.5-fold that of PNS. Meanwhile, the single-pass intestinal perfusion assay proved the absorption parameter Peff of PLZ-NPs was 1.75 and 1.80 times higher than that of PNS in the ileum and jejunum, respectively. Finally, the in vivo pharmacokinetic experiment showed that the relative oral bioavailability of PLZ-NPs was 1.71-fold that of free PNS in SD rat. In summary, the employment of the Lecithin/Zein hybrid nanoparticles could be considered as a promising approach for PNS analogues. [ABSTRACT FROM AUTHOR]

The tensile force on the hanger cables of a suspension bridge is an important indicator of the structural health of the bridge. Tensile force estimation methods based on the measured frequency of the hanger cable have been widely used. These methods empirically pre-determinate the corresponding model order of the measured frequency. However, because of the uncertain flexural rigidity, this empirical order determination method not only plays a limited role in high-order frequencies, but also hinders the online cable force estimation. Therefore, we propose a new method to automatically identify the corresponding model order of the measured frequency, which is based on a Markov chain Monte Carlo (MCMC)-based Bayesian approach. It solves the limitation of empirical determination in the case of large flexural rigidity. The tensile force and the flexural rigidity of cables can be calculated simultaneously using the proposed method. The feasibility of the proposed method is validated via a numerical study involving a finite element model that considers the flexural rigidity and via field application to a suspension bridge. [ABSTRACT FROM AUTHOR]

In an optically pumped semiconductor disc laser, which is always with a multiple layer active region and a large spot size, the spontaneous emission of quantum wells will be trapped by the waveguide caused by the multiple layer, travel a long excited distance relative to the surface-emitting mode and be subsequently amplified. Here we demonstrate a new mechanism for the amplified spontaneous emission (ASE): the etalon effect of the sub-cavity formed by the bottom distributed Bragg reflector and the top air–semiconductor interface. The spectral characteristics of this ASE including its temperature dependence, pump power dependence and observing angle dependence are experimentally studied, and the results are in good agreement with the theoretical model. [ABSTRACT FROM AUTHOR]

[Display omitted] Most anticancer drugs are not orally bioavailable due to their undesirable physicochemical properties and inherent physiological barriers. In this study, a polymeric prodrug strategy was presented to enhance the oral bioavailability of BCS class IV drugs using paclitaxel (PTX) as the model drug. PTX was covalently conjugated with cholic acid-functionalized PEG by a redox-sensitive disulfide bond. Cholic acid-functionalized PEGylated PTX (CPP) achieved remarkably improved PTX solubility (>30,000-fold), as well as favorable stability under the physiological environment and controlled drug release in the tumor. Meanwhile, CPP could self-assemble into nanoparticles with an average size of 56.18 ± 2.06 nm and drug loading up to 17.6% (w/w). Then, permeability study on Caco-2 cell monolayers demonstrated that CPP obtained an approximately 4-fold increase by apical sodium-dependent bile acid transporter (ASBT) mediated transport, compared with Taxol®. Pharmacokinetic studies carried out in rats confirmed that the oral bioavailability of CPP was 10-fold higher than that of Taxol®. Finally, significant improvement in the antitumor efficacy of CPP against breast cancer was confirmed on MDA-MB-231 cells. In summary, this prodrug-based cascade strategy offers new ways for chemotherapeutic drugs whose oral delivery is limited by solubility and permeability, also endows drugs with the capacity of tumor-specific release. [ABSTRACT FROM AUTHOR]

Osteosarcoma, a highly aggressive malignant tumor of the bone, usually occurs in children and young adults. However, although the considerable achievement in the clinical treatment of osteosarcoma recent years, the overall survival of osteosarcoma patients has not been obviously improved. Cancer cells preferentially use glycolysis instead of oxidative phosphorylation to meet their increased energetic and biosynthetic demands, a phenomenon known as the Warburg effect. Glycolysis is a driving factor in multiple cancers and is emerging as a new cancer target treatment. In the present study, we established a model to screen for glycolysis-associated genes in osteosarcoma. This risk score of the model were correlated with clinical characteristics osteosarcoma patients. Besides, a functional assay identified that STC2 enhanced the glycolysis of osteosarcoma cells. Modulation of STC2 changes glucose consumption and lactate production as well as GLUT1 expression in osteosarcoma. Furthermore, we identified that change in the expression levels of STC2 affected the proliferation, invasion, and migration of osteosarcoma cells. Our findings showed STC2 as a new tumor-promoting factor of osteosarcoma cells through enhancing glycolysis. • A prognostic glycolysis-related risk model in osteosarcoma is established. • STC2 can be used as a risk factor to predict the prognosis of patients in osteosarcoma. • STC2 improves the proliferation and induces the apoptosis in osteosarcoma cells. • STC2 influences the progression of osteosarcoma through glycolytic pathways. [ABSTRACT FROM AUTHOR]

Long noncoding RNAs (LncRNAs) show dysregulation in a variety of cancers. However, the function and specific mechanism of LncRNA GSEC in the progression of osteosarcoma remain mostly unknown. In this study, we sought to elucidate the role and mechanism of LncRNA GSEC in the occurrence and progression of osteosarcoma. we examined the expression of LncRNA GSEC in osteosarcoma cell lines by quantitative real time PCR. In vitro experiments, including transwell assays, cck8 assays, and flow cytometry analysis have biologically demonstrated the effect of LncRNA GSEC on the proliferation and migration of osteosarcoma cell lines. Furthermore, the regulation of miR-588 by LncRNA GSEC was determined by luciferase reporter assay and quantitative real time PCR. What's more, subcutaneous tumor formation was performed in nude mice to monitor the growth of the tumor in vivo. We found that the expression of LncRNA GSEC was up-regulated in osteosarcoma cell lines. Overexpression of LncRNA GSEC promoted the proliferating and migratory capacity, and inhibited the apoptosis of osteosarcoma cells. Conversely, knockdown of LncRNA GSEC resulted in the opposite effect. Mechanistically, we identified LncRNA GSEC functioned as the sponge of miR-588, thus inhibiting the miR-588/EIF5A2 signal pathway. In addition, the expression of miR-588 was negatively correlated with LncRNA GSEC, and the effect by silencing or overexpressing LncRNA GSEC could be rescued by the introduction of miR-588 mimics or inhibitors, respectively. In summary, this study shows that LncRNA GSEC promotes the proliferation and invasion of OS through the regulation of miR-588/EIF5A2 pathway, which might provide a new strategy for the treatment of osteosarcoma in the future. Image 1 • Bioinformatics prediction combined with cell experiments to verify the function of LncRNA GSEC in osteosarcoma cells. • Bioinformatics combined with double luciferase reporting experiment to reveals the downstream targets of LncRNA GSEC. • The role of LncRNA GSEC in the malignantprogression of osteosarcoma was revealed byexperiments in vivo and in vitro. [ABSTRACT FROM AUTHOR]

Abstract: A method for the simulation and optimization of the source-field distribution of a diode bar laser (DBL) beam by using the stimulated annealing and experimental data in the far field is proposed and illustrated by an example of a DBL JOLD-60-CPNN-1L, showing the applicability of the method. As compared with the previous models, this method is not restricted to the type of diode lasers (DLs), and the reconstructed results are accurate so long as the experimental data are sufficiently accurate. [Copyright &y& Elsevier]

Adenosylhomocysteinase (AHCY), a key enzyme in the methionine cycle, is essential for the development of embryos and the maintenance of mouse embryonic stem cells (mESCs). However, the precise underlying mechanism of Ahcy in regulating pluripotency remains unclear. As the only enzyme that can hydrolyze S-adenosylhomocysteine in mammals, AHCY plays a critical role in the metabolic homeostasis, epigenetic remodeling, and transcriptional regulation. Here, we identified Ahcy as a direct target of OCT4 and unveiled that AHCY regulates the self-renewal and differentiation potency of mESCs through multiple mechanisms. Our study demonstrated that AHCY is required for the metabolic homeostasis of mESCs. We revealed the dual role of Ahcy in both transcriptional activation and inhibition, which is accomplished via the maintenance of H3K4me3 and H3K27me3, respectively. We found that Ahcy is required for H3K4me3-dependent transcriptional activation in mESCs. We also demonstrated that AHCY interacts with polycomb repressive complex 2 (PRC2), thereby maintaining the pluripotency of mESCs by sustaining the H3K27me3-regulated transcriptional repression of related genes. These results reveal a previously unrecognized OCT4-AHCY-PRC2 axis in the regulation of mESCs' pluripotency and provide insights into the interplay between transcriptional factors, cellular metabolism, chromatin dynamics and pluripotency regulation., (© Crown copyright 2023.)

Background: Osteosarcoma has become the most common bone malignancy in adolescents. Although the clinical treatment of osteosarcoma has advanced considerably in recent years, the 5-year survival rate has not improved significantly. Recently, many studies have shown that mRNA has unique advantages as a target for drug therapy. Therefore, this study aimed to identify a new prognostic factor and provide a new target for the treatment of osteosarcoma to improve the prognosis of patients., Methods and Results: We selected prognostic genes that are closely associated with osteosarcoma clinical features by obtaining osteosarcoma patient information from the GTEx and TARGET databases, and then we developed a risk model. We detected the expression of FKBP11 in osteosarcoma by qRT-PCR, western blotting, and immunohistochemistry and performed CCK-8, Transwell, colony formation, and flow cytometry assays to reveal the regulatory role of FKBP11. We found that FKBP11 was highly expressed in osteosarcoma; silencing FKBP11 expression suppressed the invasion and migration of osteosarcoma cells, slowed cell proliferation, and promoted apoptosis. We also found that silencing the expression of FKBP11 led to inhibition of MEK/ERK phosphorylation., Conclusions: In conclusion, we validated that the prognostic factor FKBP11 is closely associated with osteosarcoma. Additionally, we identified a novel mechanism by which FKBP11 ameliorates the malignant properties of osteosarcoma cells through the MAPK pathway and serves as a prognostic factor in osteosarcoma. This study provides a new method for the treatment of osteosarcoma.

Background: Osteosarcoma (OS) is a common disease in the world, and its pathogenesis is still unclear. This study aims to identify the key genes that promote the proliferation, invasion, and metastasis of osteosarcoma cells., Method: GSE124768 and GSE126209 were downloaded from the Gene Expression Omnibus (GEO) database. The gene ontology and enrichment pathway were analyzed by FunRich software. qPCR and Western blot were used to detect the gene expression. After gene knockdown, Transwell and wound healing assays were conducted on osteosarcoma cells to detect whether the genes were defined before enhancing the invasion of osteosarcoma., Results: Totally, 341 mRNAs were found to be regulated differentially in osteosarcoma cells compared to osteoblasts. In addition, the expression level of Serglycin (SRGN) in osteosarcoma cells was higher than that in human osteoblasts. The invasion and proliferation ability of osteosarcoma cells with upregulated Serglycin was significantly increased, and on the contrary, decreased after Serglycin knockdown. Moreover, we preliminarily found that Serglycin may associate with the JAK/STAT signaling pathway., Conclusions: By using microarray and bioinformatics analyses, differently expressed mRNAs were identified and a complete gene network was constructed. To our knowledge, we describe for the first time Serglycin as a potential biomarker.

Background: WT161, as a selective HDAC6 inhibitor, has been shown to play anti-tumor effects on several kinds of cancers. The aim of the present study is to explore the roles of WT161 in osteosarcoma and its underlying mechanisms., Methods: The anti-proliferative effect of WT161 on osteosarcoma cells was examined using MTT assay and colony formation assay. Cell apoptosis was analyzed using flow cytometer. The synergistic effect was evaluated by isobologram analysis using CompuSyn software. The osteosarcoma xenograft models were established to evaluate the anti-proliferative effect of WT161 in vivo., Results: WT161 suppressed the cell growth and induced apoptosis of osteosarcoma cells in a dose- and time-dependent manner. Mechanistically, we found that WT161 treatment obviously increased the protein level of PTEN and decreased the phosphorylation level of protein kinase-B (AKT). More importantly, WT161 showed synergistic inhibition with 5-FU on osteosarcoma cells in vitro and in vivo., Conclusions: These results indicate that WT161 inhibits the growth of osteosarcoma through PTEN and has a synergistic efficiency with 5-FU., (© 2021 The Author(s).)