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The biochemical identification kit for Salmonella (EasyID) developed in the laboratory was evaluated. A total of 49 bacterial strains (including 12 standard strains and 16 isolates of Salmonella, 8 standard strains of Shigella, 1 standard strain and 8 isolates of Citrobacter, 1 standard strain and 2 isolates of Proteus, and 1 standard strain of Enterobacter aerogenes) were used to test four Salmonella identification kits: EasyID, traditional liquid biochemical tube (HKM), and other domestic brands (GSA and GSB). For the 49 tested strains, the identification results of three kits (EasyID, GSA and GSB) were compared with those of the HKM traditional liquid identification kit, and the overall coincidence rates were 100%, 94.46%, and 95.10% respectively. Compared with GSA and GSB, EasyID had a higher coincidence rate with HKM. Compared with the Bergey's manual, the four identification kits EasyID, HKM, GSA, and GSB yielded overall coincidence rates of 97.96%, 97.96%, 93.00%, and 94.70%, respectively. EasyID and HKM had the highest coincidence rate, followed by GSB, whereas the coincidence rate of GSA was slightly lower. Conclusion: The reliability of the EasyID Salmonella identification kit was consistent with that of traditional biochemical tubes. Furthermore, EasyID, which uses a one-step sampling technology, was easier to use than HKM, GSA, and GSB. [ABSTRACT FROM AUTHOR]

We developed a new efficient method for the synthesis of important indoxyl glycoside substrates for β-glucosidase and β-galactosidase by using 1-acetylindol-3-ones as intermediates. This method was used to synthesise novel precipitating fluorogenic substrates for β-glucosidase based on 2-(benzothiazol-2′-yl)-phenols. We also assessed the application of these substrates in the detection of foodborne pathogenic bacteria. [ABSTRACT FROM AUTHOR]

We developed a new efficient method for the synthesis of important indoxyl glycoside substrates for β-glucosidase and β-galactosidase by using 1-acetylindol-3-ones as intermediates. This method was used to synthesise novel precipitating fluorogenic substrates for β-glucosidase based on 2-(benzothiazol-2'-yl)-phenols. We also assessed the application of these substrates in the detection of foodborne pathogenic bacteria.

An improved Helferich method is presented. It involves the glycosylation of 4-methyl-umbelliferone with glycosyl acetates in the presence of boron trifluoride etherate combined with triethylamine, pyridine, or 4-dimethylaminopyridine under mild conditions, followed by deprotection to give fluorogenic 4-methylumbelliferyl glycoside substrates. Due to the use of base, the glycosylation reaction proceeds more easily, is uncommonly α- or β-stereoselective, and affords the corresponding products in moderate to excellent yields (51%-94%) under appropriate conditions.