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The coronavirus disease 2019 epidemic has prompted all universities to completely change their teaching in a very rapid way around the world. In France, most of the university courses for the year 2020 had to be delivered at distance. This can be an opportunity for some to rethink the place and the form of distance learning (DL). We present here the feedback of students about DL, who answered a questionnaire about classes they followed between October and December 2020. In addition, we evaluated the use and utility of an audience response system promoting interaction. Our results showed that overall the organization and internet connections allowed DL to run smoothly. Interestingly, all students were in favor of the development of a mixed solution, with distant lectures and face-to-face practical lessons. Finally, the use of interactive software such as Wooclap® has proven to be easy to use and to develop for all lessons.

This manuscript explores how incorporating temporal dimensions into built environment research can promote a more circular society, adding societal improvements to efficiency-driven measures closing waste or material cycles. The current circularity approaches in industrial environments mainly focus on short-term innovations reducing resource extraction and waste, overlooking long-term circularity potentials of natural resource management such as living soils as a basis for all life. This study addresses this gap by investigating, analyzing, and drawing interplays between regenerative soil cycles and business development cycles in two Flemish industry parks, Kortrijk-Noord and Haasrode. Using diachronic mapping, a qualitative design and action research tool, the study aims to generate a space–time composite of soil and business cycles, integrating archival research, interviews, and policy document reviews. This method visually captures interplays between geology, land valuation, and economic development, demonstrating that integrating soil and business cycles can suggest new pathways for site-specific circular practices on Flemish industry parks, which can inform site-specific project frameworks for circular built environments. As such, the research advocates a paradigm shift in industry park (re)development, from product and material innovation within a 'time is money' framework to an integrated 'time is life' approach, where time's historical and social dimensions are part of circular landscape development. [ABSTRACT FROM AUTHOR]

This article tackles contextual challenges in spatial plans and circularity strategies in the built environment. It highlights the issue of using generic circularity toolboxes with limited consideration of site-specific contexts. To address this, the paper proposes socio-spatial visualizations as an alternative approach. Two case studies of industry parks demonstrate the effectiveness of this method. By employing socio-spatial visualizations, future design projects can better understand and integrate site-specific circularity potential, bridging the gap between generic circularity concepts and specific contexts. The aim is to facilitate the implementation of circular economy principles while addressing contextual challenges in the built environment. [ABSTRACT FROM AUTHOR]

Escherichia coli is both a highly prevalent commensal and a major opportunistic pathogen causing bloodstream infections (BSI). A systematic analysis characterizing the genomic determinants of extra-intestinal pathogenic vs. commensal isolates in human populations, which could inform mechanisms of pathogenesis, diagnostic, prevention and treatment is still lacking. We used a collection of 912 BSI and 370 commensal E. coli isolates collected in France over a 17-year period (2000–2017). We compared their pangenomes, genetic backgrounds (phylogroups, STs, O groups), presence of virulence-associated genes (VAGs) and antimicrobial resistance genes, finding significant differences in all comparisons between commensal and BSI isolates. A machine learning linear model trained on all the genetic variants derived from the pangenome and controlling for population structure reveals similar differences in VAGs, discovers new variants associated with pathogenicity (capacity to cause BSI), and accurately classifies BSI vs. commensal strains. Pathogenicity is a highly heritable trait, with up to 69% of the variance explained by bacterial genetic variants. Lastly, complementing our commensal collection with an older collection from 1980, we predict that pathogenicity continuously increased through 1980, 2000, to 2010. Together our findings imply that E. coli exhibit substantial genetic variation contributing to the transition between commensalism and pathogenicity and that this species evolved towards higher pathogenicity. Author summary: Escherichia coli is a usually harmless bacteria typically found in the human gut. However, certain strains can cause severe bloodstream infections (BSIs) with high mortality rates, especially in older and fragile patients. To develop more effective prevention and treatment measures, understanding the genetic factors that determine a strain's capacity to cause infection is essential. In this study, we analyzed 912 BSI and 370 commensal E. coli isolates collected in France over 17 years (2000–2017). Through genetic comparison, we identified notable differences in their genetic backgrounds, as well as the presence of virulence-associated genes (VAGs) and antimicrobial resistance (AMR) genes. Using a machine learning model, we distinguished BSI from commensal strains, thereby discovering genetic factors linked to pathogenicity. Our findings indicate that E. coli exhibits significant genetic variation contributing to the switch between commensalism and pathogenesis. We also suggest that pathogenicity in France has steadily increased over time, presenting a potentially serious public health threat. Our work is an important step in guiding future research to enhance diagnostic, prevention, and treatment strategies. [ABSTRACT FROM AUTHOR]

The fight against antibiotic resistance has become a true global public health challenge of gargantuan proportions. Amongst the myriad of approaches being explored to tackle this predicament, one strategy involves enhancing prescriber knowledge and in particular their basic knowledge of medical bacteriology. Yet, as we well know in medical microbiology teachings, traditional lectures can be arduous, attempting to cram in a vast array of information in a limited time. An alternative solution to improve student engagement and enhance learning outcomes is to utilize educational games in complementary approach. Such games are an effective means of inspiring students to learn, encouraging self-assessment, and injecting diversity into the teaching process. To this end, we have developed and evaluated an educational card game, the 'BacteriaGame,' aimed at our medical students in medical bacteriology. Designed for students at the basic level, it serves as activity at the end of their apprenticeship to their bacteriology education. Additionally, it can also be used as a review tool by more advanced students, with teachers able to impart additional knowledge as the game progresses. We also use it in continuous training of medical laboratory staff. In this study, we evaluated the game at various stages of medical education, collecting feedback and analysing its impact on knowledge acquisition, comparing it to traditional lectures. Feedback from the majority of students revealed that the rules were clear, the game was enjoyable, and neither too lengthy nor too challenging. The integration of 'BacteriaGame' into their future training piqued their interest. In terms of learning outcomes, we discovered a significant increase in knowledge acquisition among those who used the game (P  < .05). 'BacteriaGame' is now published by the French Society of Microbiology (SFM) and distributed in all medical and pharmacy schools thanks to a funding of the French Health Ministry. An English edition of the game is also available for international use as a physical copy to be purchased from the SFM. This will allow a large-scale distribution to colleagues who would like to use this game in their teaching. [ABSTRACT FROM AUTHOR]

Comics are a known method to visually link characters to context through time. This article explores the medium of comics to untangle stakeholder dynamics in the context of a complex theme such as circularity of the built environment. Circularity of the built environment tailors concepts of circular economy to the field of construction and urban development. Relying mostly on optimization strategies, context-specific characteristics such as stakeholder agency and spatial preconditions are often disregarded as resources in the design of circularity projects. This results in one-size-fits all circularity instruments formalized in generic toolboxes. Circularity instruments should additionally engage with stakeholders, recognizing complexity and surfacing the resourcefulness of the territory. This comics series follows the researcher from analysis to design hypothesis, clarifying complexity at hand from the researcher perspective, including stakeholder agendas, spatial conditions, barriers and opportunities. Part of an ongoing action-research project, the self-reflective comics show parts of a researcher's journey untangling circularity in the built environment in its multiple stakeholder dimensions. It includes data sourced from mixed method research, such as ethnographic fieldwork, semi-structured interviews, and archival research on two Flemish industry parks, Kortrijk-Noord and Leuven-Haasrode. These comics function as a narrative assemblage method for critical analysis, bringing together different data sources, and rendering our research process on circularity contextual and visual. Additionally, the comic allows us to communicate, challenge, and begin to design with (hidden) stakeholder agency. [ABSTRACT FROM AUTHOR]

This paper aims to apply an intersectional environmentalist lens to the circular economy (CE) transition in Flanders. CE discourse often takes a deterritorialised approach, that is, a focus on innovation and growth. This approach tends to neglect local knowledge and background skills that can inhabit and work with landscapes in balanced ways to enable a fully circular society. This knowledge is partly embodied by "nobodied" actors. After introducing relevant terminology, this article draws upon a collaborative autoethnography which integrates autobiographies of authors' experiences of circularity in projects with "nobodied" CE actors, and ethnographic notes on the Flemish context in which the CE discourse developed. The reflections unearth how a lack of an intersectional environmentalist lens in the CE rhetoric "nobodies" informal CE actors and practices. We show how they do not matter in a circular economy in a deterritorialised approach, but how they matter in a circular landscape view. [ABSTRACT FROM AUTHOR]

Escherichia coli is a commensal species of the lower intestine but is also a major pathogen causing intestinal and extraintestinal infections that is increasingly prevalent and resistant to antibiotics. Most studies on genomic evolution of E. coli used isolates from infections. Here, instead, we whole-genome sequenced a collection of 403 commensal E. coli isolates from fecal samples of healthy adult volunteers in France (1980 to 2010). These isolates were distributed mainly in phylogroups A and B2 (30% each) and belonged to 152 sequence types (STs), the five most frequent being ST10 (phylogroup A; 16.3%), ST73 and ST95 (phylogroup B2; 6.3 and 5.0%, respectively), ST69 (phylogroup D; 4.2%), and ST59 (phylogroup F; 3.9%), and 224 O:H serotypes. ST and serotype diversity increased over time. The O1, O2, O6, and O25 groups used in bioconjugate O-antigen vaccine against extraintestinal infections were found in 23% of the strains of our collection. The increase in frequency of virulence-associated genes and antibiotic resistance was driven by two evolutionary mechanisms. Evolution of virulence gene frequency was driven by both clonal expansion of STs with more virulence genes ("ST-driven") and increases in gene frequency within STs independent of changes in ST frequencies ("gene-driven"). In contrast, the evolution of resistance was dominated by increases in frequency within STs ("gene-driven"). This study provides a unique picture of the phylogenomic evolution of E. coli in its human commensal habitat over 30 years and will have implications for the development of preventive strategies. [ABSTRACT FROM AUTHOR]

The Seine-Saint-Denis is the French metropolitan department with the highest incidence of tuberculosis (TB). Our aim was to explore epidemiological and phylogenetic characteristics of TB strains in this hotspot department. We performed WGS on 227 strains of Mycobacterium tuberculosis complex isolated from patients at the Avicenne Hospital from 2016 to 2021 and randomly selected to represent the clinical diversity of French TB localization. Clinical and demographic data were recorded for each TB patient. The mean age of patients was 36 years old. They came from Africa (44%), Asia (27%), Europe (26%) and America (3%). Strains isolated from extrapulmonary samples were associated with Asian patients, whereas strains isolated from pulmonary samples were associated with European patients. We observed a high level of lineage diversity in line with the known worldwide diversity. Interestingly, lineage 3 was associated with lymph node TB. Additionally, the sensitivity of WGS for predicting resistance was 100% for rifampicin, isoniazid and ethambutol and 66.7% for pyrazinamide. The global concordance with drug-susceptibility testing using the phenotypic approach was 97%. In microbiology laboratories, WGS turns out to be an essential tool for better understanding local TB epidemiology, with direct access to circulating lineage identification and to drug susceptibilities to first- and second-line anti-TB drugs. [ABSTRACT FROM AUTHOR]

The central nervous system is the location of metastases in more than 40% of patients with lung cancer, breast cancer and melanoma. These metastases are associated with one of the poorest prognoses in advanced cancer patients, mainly due to the lack of effective treatments. In this review, we explore the involvement of cytokines, including interleukins and chemokines, during the development of brain and leptomeningeal metastases from the epithelial-to-mesenchymal cell transition and blood–brain barrier extravasation to the interaction between cancer cells and cells from the brain microenvironment, including astrocytes and microglia. Furthermore, the role of the gut–brain axis on cytokine release during this process will also be addressed. [ABSTRACT FROM AUTHOR]

This paper constructs an alternative history of wood in Leuven (Belgium) to help contextualize the city's contemporary project for a building materials bank as part of their larger efforts to make the urban metabolism more circular. Focusing on the history of Leuven's forests, canal, and deconstruction practices, this paper aims to show how the materials bank will also intervene into the larger overlapping timber flows of the region. By analysing how (circular) wood flows were strongly intertwined with urban and landscape development projects in pre-industrial Leuven, the paper speculates on how the materials bank could revitalize broken spatial connections towards more circular timber flows in Leuven, while catalysing circular urban landscape and infrastructure development. It shifts focus from a materials bank as a circular waste management response "redirecting" wood waste flows to an integrated infrastructural question addressing path dependencies in the wood extraction, processing, consumption and disposal chain. [ABSTRACT FROM AUTHOR]

The process of species diversification is traditionally summarized by a single tree, the species tree, whose reconstruction from molecular data is hindered by frequent conflicts between gene genealogies. Here, we argue that instead of seeing these conflicts as nuisances, we can exploit them to inform the diversification process itself. We adopt a gene‐based view of diversification to model the ubiquitous presence of gene flow between diverging lineages, one of the most important processes explaining disagreements among gene trees. We propose a new framework for modelling the joint evolution of gene and species lineages relaxing the hierarchy between the species tree and gene trees inherent to the standard view, as embodied in a popular model known as the multispecies coalescent (MSC). We implement this framework in two alternative models called the gene‐based diversification models (GBD): (a) GBD‐forward following all evolving genomes through time and (b) GBD‐backward based on coalescent theory. They feature four parameters tuning colonization, gene flow, genetic drift and genetic differentiation. We propose an inference method based on differences between gene trees. Applied to two empirical data sets prone to gene flow, we find better support for the GBD‐backward model than for the MSC model. Along with the increasing awareness of the extent of gene flow, this work shows the importance of considering the richer signal contained in genomic histories, rather than in the mere species tree, to better apprehend the complex evolutionary history of species. [ABSTRACT FROM AUTHOR]

Byline: Marie-France B. Turcotte, Stewart R. Clegg, Julie Marin The article presents the case study of a partnership between a metallurgy company and an NGO concerned with environmental protection. The partnership constituted an attempt to reconcile the firm's economic objectives with those of the citizens who lived in the area on which it had an ecological impact. Driven by high ideals, the multistakeholder partnerships were an innovation inspired by the ideal speech situation theory and a focus on learning and innovation. The partnership seemingly created an arena defined by norms of 'disinterested rationality' with an objective of innovating and progressing toward sustainable development. The partnership had only a marginal influence on the firm's activities, which were mainly determined by market forces and economic logic. The article concludes with a rather critical perspective on the outcomes of the case in terms of learning, innovation and change, with a theoretical lens inspired by theories on learning, legitimacy and power. The article contributes to the understanding and definition of legitimacy in a polyphonic context, where different views coexist or confront. Legitimacy is neither an outside nor static institutional feature, but rather resembles a kaleidoscope of perceptions that are defined, temporarily granted and redefined through discursive interactions. In such a context, moral arguments are confronted with other moral arguments while actors redefine their knowledge and cognitive frameworks. Practical recommendations are formulated for the convenors of multistakeholders partnerships, activist groups and firms.

The phylogenetic relationships between the three main clades of worm snakes remain controversial. This question is, however, crucial to elucidate the origin of the successful snake radiation, as these burrowing and miniaturized wormlike organisms represent the earliest branching clades within the snake tree. The present molecular phylogenetic study, intended to minimize the amount of missing data, provides fully resolved inter‐subfamilial relationships among Typhlopidae. It also brings robust evidence that worm snakes (Scolecophidia) are paraphyletic, with the scolecophidian family Anomalepididae recovered with strong support as sister clade to the 'typical snakes' (Alethinophidia). Ancestral state reconstructions applied to three different traits strongly associated to a burrowing life‐style (scolecoidy, absence of retinal cones and microstomy) provide results in favour of a burrowing origin of snakes, and suggest that worm snakes might be the only extant fossorial representatives of the primordial snake incursion towards an underground environment. [ABSTRACT FROM AUTHOR]

Genomic data drive evolutionary research on the relationships and timescale of life but the genomes of most species remain poorly sampled. Phylogenetic trees can be reconstructed reliably using small data sets and the same has been assumed for the estimation of divergence time with molecular clocks. However, we show here that undersampling of molecular data results in a bias expressed as disproportionately shorter branch lengths and underestimated divergence times in the youngest nodes and branches, termed the small sample artifact. In turn, this leads to increasing speciation and diversification rates towards the present. Any evolutionary analyses derived from these biased branch lengths and speciation rates will be similarly biased. The widely used timetrees of the major species-rich studies of amphibians, birds, mammals, and squamate reptiles are all data-poor and show upswings in diversification rate, suggesting that their results were biased by undersampling. Our results show that greater sampling of genomes is needed for accurate time and rate estimation, which are basic data used in ecological and evolutionary research. [ABSTRACT FROM AUTHOR]

The increasing size of timetrees in recent years has led to a focus on diversification analyses to better understand patterns of macroevolution. Thus far, nearly all studies have been conducted with eukaryotes primarily because phylogenies have been more difficult to reconstruct and calibrate to geologic time in prokaryotes. Here, we have estimated a timetree of 11,784 'species' of prokaryotes and explored their pattern of diversification. We used data from the small subunit ribosomal RNA along with an evolutionary framework from previous multi-gene studies to produce three alternative timetrees. For each timetree we surprisingly found a constant net diversification rate derived from an exponential increase of lineages and showing no evidence of saturation (rate decline), the same pattern found previously in eukaryotes. The implication is that prokaryote diversification as a whole is the result of the random splitting of lineages and is neither limited by existing diversity (filled niches) nor responsive in any major way to environmental changes. [ABSTRACT FROM AUTHOR]

The worm-like snakes ( Scolecophidia; approximately 400 nominal extant species) have a conservative morphology and are among the most poorly-known terrestrial vertebrates. Although molecular evidence has helped determine their higher-level relationships, such data have rarely been used to discriminate among species. We generated a molecular data set for the continental Australian blindsnakes (genus Ramphotyphlops) to determine the concordance of molecular and morphological information in the taxonomic recognition of species. Our dataset included 741 specimens morphologically attributed to 27 nominal Ramphotyphlops species. We proposed species hypotheses ( SHs) after analysis of sequences from a variable mitochondrial gene ( cytochrome b) and examined these SHs with additional evidence from a nuclear gene ( prolactin receptor) and geographical data. Although the nuclear marker was not as fast-evolving and discriminating as the mitochondrial marker, there was congruence among the mitochondrial, nuclear, and geographical data, suggesting that the actual number of species is at least two times the current number of recognized, nominal species. Several biogeographical barriers and complex phytogeographical and geological patterns appeared to be involved in the division of some burrowing snake populations and, by consequence, in their diversification and speciation through isolation. © 2013 The Linnean Society of London, Biological Journal of the Linnean Society, 2013, 110, 427-441. [ABSTRACT FROM AUTHOR]

Background: The arylamine N-acetyltransferases (NATs) are a unique family of enzymes widely distributed in nature that play a crucial role in the detoxification of aromatic amine xenobiotics. Considering the temporal changes in the levels and toxicity of environmentally available chemicals, the metabolic function of NATs is likely to be under adaptive evolution to broaden or change substrate specificity over time, making NATs a promising subject for evolutionary analyses. In this study, we trace the molecular evolutionary history of the NAT gene family during the last ~450 million years of vertebrate evolution and define the likely role of gene duplication, gene conversion and positive selection in the evolutionary dynamics of this family. Results: A phylogenetic analysis of 77 NAT sequences from 38 vertebrate species retrieved from public genomic databases shows that NATs are phylogenetically unstable genes, characterized by frequent gene duplications and losses even among closely related species, and that concerted evolution only played a minor role in the patterns of sequence divergence. Local signals of positive selection are detected in several lineages, probably reflecting response to changes in xenobiotic exposure. We then put a special emphasis on the study of the last ~85 million years of primate NAT evolution by determining the NAT homologous sequences in 13 additional primate species. Our phylogenetic analysis supports the view that the three human NAT genes emerged from a first duplication event in the common ancestor of Simiiformes, yielding NAT1 and an ancestral NAT gene which in turn, duplicated in the common ancestor of Catarrhini, giving rise to NAT2 and the NATP pseudogene. Our analysis suggests a main role of purifying selection in NAT1 protein evolution, whereas NAT2 was predicted to mostly evolve under positive selection to change its amino acid sequence over time. These findings are consistent with a differential role of the two human isoenzymes and support the involvement of NAT1 in endogenous metabolic pathways. Conclusions: This study provides unequivocal evidence that the NAT gene family has evolved under a dynamic process of birth-and-death evolution in vertebrates, consistent with previous observations made in fungi. [ABSTRACT FROM AUTHOR]

Marin, J., Crouau-Roy, B., Hemptinne, J.-L., Lecompte, E. & Magro, A. (2010). Coccinella septempunctata (Coleoptera, Coccinellidae): a species complex? - Zoologica Scripta, 39, 591-602. Coccinella septempunctata L., the seven spot ladybird is widely distributed across the Paleartic region. Based on a few morphological characteristics and geographical origin, some populations are recognized as distinct species, e.g., the North African Coccinella algerica Kovář and Japanese Coccinella brucki Mulsant later considered to be a subspecies - C. septempunctata brucki. The objective of this study is to discuss the taxonomic status of the seven spot ladybird, in particular whether it is a complex of species or the same species throughout the Paleartic region. The relationship between populations was clarified by using a combination of molecular and limited morphometric data and assessing potential reproductive barriers by means of cross breeding. Although there is considerable variation in the size of the spots on the elytra, the results of this study confirm that all studied populations belong to the same species. [ABSTRACT FROM AUTHOR]

The article discusses a molecular study on a sampling of Typhlopidae, the largest family of vision handicapped snakes that look like worms called the scolecophidians. The results found that blindsnakes are widely distributed in Gondwana. Their early lineages are found in the palaeolandmass of Madagascar and India. The diversification of the scolecophidians in the Cenozoic era is linked to their source of prey, the ants and termites.

In recent years, cities have revealed themselves as being prominent actors in the circular economy transition. Besides supporting and initiating urban projects catalyzing circularity, cities are looking for monitoring tools that can make their progress towards circularity visible. Adopting Leuven's pilot project for a building materials bank as a case study, this paper notes the particular challenges and opportunities in the pilot project to assess its progress and impact, in combination with gathering data for overall circular city monitoring purposes. Firstly, the paper names tensions between the "messy" transition process from policy ambitions to implementation and the question of data and monitoring. Secondly, the paper identifies relevant dimensions and scales to evaluate progress and impacts of a building materials bank, drawing from its development process. Thirdly, it proposes guidelines to monitor and evaluate circular city projects from the bottom up, combining quantitative indicators with guiding questions in a developmental evaluation. The analysis serves a critical reflection, distills lessons learned for projects contributing to circular cities and feeds a few concluding policy recommendations. The case study serves as an example that, in order to move beyond the tensions between circularity monitoring and actual circular city project development, monitoring instruments should simultaneously interact with and feed the circularity transition process. Therefore, dedicated data governance driven by enhanced stakeholder interactions should be inscribed in transition process guidance. Bottom-up projects such as a building materials bank provide opportunities to do this. [ABSTRACT FROM AUTHOR]

Embodying circular economy transition as a sustainable city concept, circularity in cities is increasingly the subject of policy innovations, urban strategies, and research & development agendas. It seems evident that a circular city should include more than the sum or multiplication of urban circular economies. Nevertheless, prevailing discourses remain till today business focused, and how circular economy creates economic, social, and environmental resilience in cities has yet to be explored. This paper conceptualizes the notion of urban circularity. It introduces an analytical framework sorting existing circularity concepts that are based on design and planning characteristics. Adopting comparative case study research on four contemporary forward-looking spatial representations of ‘circular’ places, this paper articulates their circularity interpretation. Demonstrating how diverging sustainability framings and political positions are embedded within the studied spatial representations, this paper aims to bring clarity in contemporary circular city approaches for policymakers as well as for spatial practitioners. The paper concludes with an agenda for multi-perspective and multi-dimensional circular city design, which is anchored in place specific and multi-scalar transition relations. It suggests urban landscape design as a disciplinary field to act as a pivot in transdisciplinary circularity design and research. [ABSTRACT FROM AUTHOR]

According to evidence-based guidelines, vaccines against measles and varicella are generally recommended to susceptible HIV-positive patients, as long as they are not severely immunocompromised. However, routine screening to determine serologic status is not recommended. We conducted a seroprevalence study of anti-measles and anti-Varicella-Zoster virus (VZV) antibodies in adults living with HIV (PLWHA) consulting at Avicenne University Hospital in a Parisian suburb. Sera were collected in years 2018–2020 and tested by commercial immunoassays in 268 patients. Most of the patients were born in Sub-Saharan Africa (55 %) and only 23 % in Europe. Measles and varicella seropositivity were present respectively in 91.4 % and 96.2 % of patients. One patient in ten was seronegative to at least one of tested diseases. In the univariate analysis, only younger age (p = 0.027) was associated with a higher risk of measles seronegativity, while shorter time since arrival in France (p < 0.001) and shorter time since HIV discovery (p = 0.007) were associated with a higher risk of VZV seronegativity. In multivariate analysis no association was found. This study highlights the absence of specific risk factors for VZV and measles seronegativity in PLWHA and supports the importance of routine screening, in order to increase immunization rates and reduce risk of complications. [ABSTRACT FROM AUTHOR]

In microbiota research, whole stool sampling is the conventional approach but can be problematic or infeasible for certain patients. This study aims to validate the use of rectal swabbing as an alternative method for microbiota analysis and determine optimal storage conditions suitable for various clinical settings, including intensive care units. We evaluated different sampling techniques and storage temperatures. Our findings indicated that rectal swabs yield microbiota diversity comparable to whole stool samples. Notably, storage conditions significantly impacted microbiota profiles, with increased E. coli and Enterococcus sp . quantifications observed at room temperature (RT). Consequently, we recommend immediate refrigeration of rectal swabs to reliably assess aerobic and total microbiota, particularly for patients requiring urgent care, such as antibiotic treatment., Importance: We developed a pragmatic approach to study total and aerobic gut microbiota, applicable in numerous clinical units, such as intensive care or emergency units, where whole stool sampling is often impractical. This approach employs ESwab devices, which are already commonly used in hospitals.

The present study aimed to examine the effects of low doses of angiotensin II (AngII) on cardiac function, myocardial substrate utilization, energetics, and mitochondrial function in C57Bl/6J mice and in a transgenic mouse model with cardiomyocyte specific upregulation of NOX2 (csNOX2 TG). Mice were treated with saline (sham), 50 or 400 ng/kg/min of AngII (AngII 50 and AngII 400 ) for two weeks. In vivo blood pressure and cardiac function were measured using plethysmography and echocardiography, respectively. Ex vivo cardiac function, mechanical efficiency, and myocardial substrate utilization were assessed in isolated perfused working hearts, and mitochondrial function was measured in left ventricular homogenates. AngII 50 caused reduced mechanical efficiency despite having no effect on cardiac hypertrophy, function, or substrate utilization. AngII 400 slightly increased systemic blood pressure and induced cardiac hypertrophy with no effect on cardiac function, efficiency, or substrate utilization. In csNOX2 TG mice, AngII 400 induced cardiac hypertrophy and in vivo cardiac dysfunction. This was associated with a switch towards increased myocardial glucose oxidation and impaired mitochondrial oxygen consumption rates. Low doses of AngII may transiently impair cardiac efficiency, preceding the development of hypertrophy induced at higher doses. NOX2 overexpression exacerbates the AngII -induced pathology, with cardiac dysfunction and myocardial metabolic remodelling.

In standard models of molecular evolution, DNA sequences evolve through asynchronous substitutions according to Poisson processes with a constant rate (called the molecular clock) or a rate that can vary (relaxed clock). However, DNA sequences can also undergo episodes of fast divergence that will appear as synchronous substitutions affecting several sites simultaneously at the macroevolutionary timescale. Here, we develop a model, which we call the Relaxed Clock with Spikes model, combining basal, clock-like molecular substitutions with episodes of fast divergence called spikes arising at speciation events. Given a multiple sequence alignment and its time-calibrated species phylogeny, our model is able to detect speciation events (including hidden ones) cooccurring with spike events and to estimate the probability and amplitude of these spikes on the phylogeny. We identify the conditions under which spikes can be distinguished from the natural variance of the clock-like component of molecular substitutions and from variations of the clock. We apply the method to genes underlying snake venom proteins and identify several spikes at gene-specific locations in the phylogeny. This work should pave the way for analyses relying on whole genomes to inform on modes of species diversification., (© The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)

Monitor lizards are emblematic reptiles that are widely distributed in the Old World. Although relatively well studied in vertebrate research, their biogeographic history is still controversial. We constructed a molecular dataset for 54 anguimorph species, including representatives of all families with detailed sampling of the Varanidae (38 species). Our results are consistent with an Asian origin of the Varanidae followed by a dispersal to Africa 41 (49-33) Ma, possibly via an Iranian route. Another major event was the dispersal of monitors to Australia in the Late Eocene-Oligocene 32 (39-26) Ma. This divergence estimate adds to the suggestion that Australia was colonized by several squamate lineages prior to the collision of the Australian plate with the Asian plate starting 25 Ma.