Search

In recent years, the ever-increasing research interest in various aspects of the electricity pool-based markets has generated a plethora of complementarity-based approaches to determine participating agents’ optimal offering/bidding strategies and model players’ interactions. In particular, the integration of multiple and diversified market agents, such as conventional generation companies, renewable energy sources, electricity storage facilities and agents with a mixed generation portfolio has instigated significant competition, as each player attempts to establish their market dominance and realize substantial financial benefits. The employment of complementarity modelling approaches can also prove beneficial for the optimal coordination of the electricity and natural gas market coupling. Linear and nonlinear programming as well as complementarity modelling, mainly in the form of mathematical programs with equilibrium constraints (MPECs), equilibrium programs with equilibrium constraints (EPECs) and conjectural variations models (CV) have been widely employed to provide effective market clearing mechanisms, enhance agents’ decision-making process and allow them to exert market power, under perfect and imperfect competition and various market settlements. This work first introduces the theoretical concepts that regulate the majority of contemporary competitive electricity markets. It then presents a comprehensive review of recent advances related to complementarity-based modelling methodologies and their implementation in current competitive electricity pool-based markets applications.

Mammalian cell cultures are intrinsically heterogeneous at different scales (molecular to bioreactor). The cell cycle is at the centre of capturing heterogeneity since it plays a critical role in the growth, death, and productivity of mammalian cell cultures. Current cell cycle models use biological variables (mass/volume/age) that are non-mechanistic, and difficult to experimentally determine, to describe cell cycle transition and capture culture heterogeneity. To address this problem, cyclins-key molecules that regulate cell cycle transition-have been utilized. Herein, a novel integrated experimental-modelling platform is presented whereby experimental quantification of key cell cycle metrics (cell cycle timings, cell cycle fractions, and cyclin expression determined by flow cytometry) is used to develop a cyclin and DNA distributed model for the industrially relevant cell line, GS-NS0. Cyclins/DNA synthesis rates were linked to stimulatory/inhibitory factors in the culture medium, which ultimately affect cell growth. Cell antibody productivity was characterized using cell cycle-specific production rates. The solution method delivered fast computational time that renders the model's use suitable for model-based applications. Model structure was studied by global sensitivity analysis (GSA), which identified parameters with a significant effect on the model output, followed by re-estimation of its significant parameters from a control set of batch experiments. A good model fit to the experimental data, both at the cell cycle and viable cell density levels, was observed. The cell population heterogeneity of disturbed (after cell arrest) and undisturbed cell growth was captured proving the versatility of the modelling approach. Cell cycle models able to capture population heterogeneity facilitate in depth understanding of these complex systems and enable systematic formulation of culture strategies to improve growth and productivity. It is envisaged that this modelling approach will pave the model-based development of industrial cell lines and clinical studies.