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527 results on '"Mo, Fei"'

24. Heat stress activates YAP/TAZ to induce the heat shock transcriptome

Author

Luo, Min, Meng, Zhipeng, Moroishi, Toshiro, Lin, Kimberly C, Shen, Guobo, Mo, Fei, Shao, Bin, Wei, Xiawei, Zhang, Ping, Wei, Yuquan, and Guan, Kun-Liang

Subjects
Biochemistry and Cell Biology, Biological Sciences, Biotechnology, Aetiology, 2.1 Biological and endogenous factors, A549 Cells, Adaptor Proteins, Signal Transducing, Animals, Cell Cycle Proteins, Cell Line, Tumor, Cell Survival, Female, Gene Expression Profiling, HEK293 Cells, Heat-Shock Response, Humans, Mice, Mice, Inbred BALB C, Mice, Nude, NIH 3T3 Cells, Nuclear Proteins, Phosphoprotein Phosphatases, Phosphorylation, Protein Serine-Threonine Kinases, Signal Transduction, Trans-Activators, Transcription Factors, Transcriptional Coactivator with PDZ-Binding Motif Proteins, Transcriptome, YAP-Signaling Proteins, Medical and Health Sciences, Developmental Biology, Biochemistry and cell biology
Abstract

The Hippo pathway plays critical roles in cell growth, differentiation, organ development and tissue homeostasis, whereas its dysregulation can lead to tumorigenesis. YAP and TAZ are transcription co-activators and represent the main downstream effectors of the Hippo pathway. Here, we show that heat stress induces a strong and rapid YAP dephosphorylation and activation. The effect of heat shock on YAP is dominant to other signals known to modulate the Hippo pathway. Heat shock inhibits LATS kinase by promoting HSP90-dependent LATS interaction with and inactivation by protein phosphatase 5. Heat shock also induces LATS ubiquitination and degradation. YAP and TAZ are crucial for cellular heat shock responses, including the heat shock transcriptome and cell viability. This study uncovers previously unknown mechanisms of Hippo regulation by heat shock, as well as physiological functions of YAP, in the heat stress response. Our observations also reveal a potential combinational therapy involving hyperthermia and targeting of the Hippo pathway.

Published
2020

40. Effects of Kalimeris indica (L.) Sch Bip on colitis-associated colorectal cancer

Author

Mo-Fei Wang, Hao Li, Jian Cui, Yu-Han Chen, and Yong Cui

Subjects
colitis associated cancer, microRNA, miR-31-5p, Kalimeris indica (L.) Sch Bip, colorectal cancer, Therapeutics. Pharmacology, RM1-950
Abstract

Kalimeris indica (L.) Sch Bip (K. indica) is a plant of the genus Kalimeris in Asteraceae, and its whole herb can be used as medicine for the treatment of intestinal inflammatory diseases. But the mechanism is not clear. Therefore, this study was designed to explore the mechanism of K. indica (KI) in colitis-associated colorectal cancer. The expression levels of miR-31-5p and proinflammatory factors were detected using THP-1 and Caco2 cells in vitro. KI could rescue the upregulation of miR-31-5p induced by IL-6 and TNF-α in Caco2 and THP-1 cells. In LPS-stimulated PMA-differentiated THP-1 cells, KI restored miR-31-5p expression by downregulating the expression of IL-6 and TNF-α. C57BL/6 mice were used to construct CAC model through the induction of azoxymethane/dextran sulfate sodium. The successfully established CAC mice were treated with water extract of KI through intragastric administration for 5 weeks. The result showed that KI could significantly reduce the atypical hyperplasia in colon tissue, and inhibit the expression of proinflammatory factors such as IL-6, TNF, IL-11, IL-7, etc. At the same time, KI could restore the level of miR-31-5p in mice, and therefore the downstream LATS2 to inhibit the development of CAC. These above results indicate that KI is a potentially effective herb medicine to prevent CAC.

Published
2023
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44. An engineered IL-2 partial agonist promotes CD8.sup.+ T cell stemness

Author

Mo, Fei, Yu, Zhiya, Li, Peng, Oh, Jangsuk, Spolski, Rosanne, Zhao, Liang, Glassman, Caleb R., Yamamoto, Tori N., Chen, Yun, Golebiowski, Filip M., Hermans, Dalton, Majri-Morrison, Sonia, Picton, Lora K., Liao, Wei, Ren, Min, Zhuang, Xiaoxun, and Mitra, Suman

Subjects
Agonists (Biochemistry) -- Physiological aspects, T cells -- Physiological aspects, Stem cells -- Physiological aspects, Environmental issues, Science and technology, Zoology and wildlife conservation
Abstract

Adoptive transfer of antigen-specific T cells represents a major advance in cancer immunotherapy, with robust clinical outcomes in some patients.sup.1. Both the number of transferred T cells and their differentiation state are critical determinants of effective responses.sup.2,3. T cells can be expanded with T cell receptor (TCR)-mediated stimulation and interleukin-2, but this can lead to differentiation into effector T cells.sup.4,5 and lower therapeutic efficacy.sup.6, whereas maintenance of a more stem-cell-like state before adoptive transfer is beneficial.sup.7. Here we show that H9T, an engineered interleukin-2 partial agonist, promotes the expansion of CD8.sup.+ T cells without driving terminal differentiation. H9T led to altered STAT5 signalling and mediated distinctive downstream transcriptional, epigenetic and metabolic programs. In addition, H9T treatment sustained the expression of T cell transcription factor 1 (TCF-1) and promoted mitochondrial fitness, thereby facilitating the maintenance of a stem-cell-like state. Moreover, TCR-transgenic and chimeric antigen receptor-modified CD8.sup.+ T cells that were expanded with H9T showed robust anti-tumour activity in vivo in mouse models of melanoma and acute lymphoblastic leukaemia. Thus, engineering cytokine variants with distinctive properties is a promising strategy for creating new molecules with translational potential. H9T, an engineered IL-2 partial agonist, promotes the expansion of T cells while maintaining a stem-cell-like state, leading to improved efficacy of adoptive cell therapy in mouse models of melanoma and acute lymphoblastic leukaemia., Author(s): Fei Mo [sup.1] , Zhiya Yu [sup.2] , Peng Li [sup.1] , Jangsuk Oh [sup.1] , Rosanne Spolski [sup.1] , Liang Zhao [sup.3] , Caleb R. Glassman [sup.4] , [...]

Published
2021
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