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A Thlaspi caerulescens (J. & C. PRESL) was used to study the effect of cadmium on the content of free amino acids and ability accumulation of Cd in ecotypes of this plant species. In pot experiment two ecotypes T. caerulescens were used: Ganges ecotype from France and Mežica ecotype from Slovenia. The plants were grown in soil (chernozem – Suchdol) spiked with NPK and three different concentration of Cd: 30, 60 and 90 mg/kg. The content of Cd was measured in the above-ground biomass and roots using ICP-OES. Accumulation of Cd was higher in the Mežica ecotype in contrast to the low Cd-accumulating the Ganges ecotype. Analyses of free amino acids contents were measured by GC-MS method. The content of free amino acids in above-ground biomass of the Mežica ecotype declined progressively with increasing concentrations of Cd. Opposite trend was observed in roots of this ecotype. The increase of free amino acids contents in above-ground biomass and roots of the Ganges ecotype were detected. The results of specific amino acids free proline showed increased content in plant biomass with increasing Cd contamination of soil. A statistically significant increase was observed between control plants (0 mg/kg Cd) and variant Cd3 (90 mg/kg Cd) for both ecotypes. The statistically significant decrease of free proline was observed in the Mežica ecotype roots. Opposite trend was observed in roots of Ganges ecotype - increasing trend of free proline content. These results indicate a correlation between content of Cd and content of free proline in different parts of the plant. We can speculate that the mechanism of Cd hyperaccumulation and metabolism of free proline are not identical in ecotypes of this species.

The removal of copper (Cu) in soils by green technology is less treated with urgency, as it is a plant micronutrient. We examined the efficiency of Cu shoot accumulation by herbaceous plants in Cu-contaminated and non-contaminated soils in Trhové Dusniky and Podles, respectively, in the Czech Republic. The total soil Cu content of 81 mg kg -1 in Trhové Dusniky indicated a slight contamination level compared to 50 mg kg -1 , the permissible value by WHO, and < 35 in Podlesí, representing a clean environment. The Cu content was above the permissible value in plants (10 mg kg -1 by WHO) in herbaceous speciesat the control site without trees: Stachys palustris L. (10.8 mg kg -1 ), Cirsium arvense L. (11.3 mg kg -1 ), Achillea millefolium L. (12.1 mg kg -1 ), Anthemis arvense L. (13.2 mg kg -1 ), and Calamagrostis epigejos L. (13.7 mg kg -1 ). In addition, Hypericum maculatum Crantz (10.6 mg kg -1 ), Campanula patula L. (11.3 mg kg -1 ), C. arvense (15 mg kg -1 ), and the highest accumulation in shoot of Equisetum arvense L. (37.1 mg kg -1 ), all under the canopy of trees at the uncontaminated site, were above the WHO value. Leucanthemum Vulgare (Lam.) and Plantago lanceolata L. recorded 11.2 mg kg -1 and 11.5 mg kg -1 , respectively, in the soil of the Cu-contaminated site. These herbaceous species can support the phyto-management of Cu-contaminated soils, especially E. arvense. Critical attention is well-required in the medicinal application of herbaceous plants in treating human ailments due to their Cu accumulation potentials above the threshold. Spontaneous surveys and analysis of Cu speciation in herbaceous species can reveal suitable plants to decontaminate soils and provide caution on consumable products, especially bioactive compounds., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Two groups of invertebrates, terrestrial mollusks (Gastropoda) and Chrysomela populi leaf beetles (Coleoptera: Chrysomelidae), were sampled to estimate the suitability of these organisms as bioindicators of risk element pollution (predominantly Cd, Pb, and Zn) alongside the risk element concentration gradient in the contaminated area (former mining/smelting areas in the vicinity of Příbram city, Central Bohemia, Czech Republic). The individuals representing ten species of terrestrial snails and imagoes of C. populi were collected manually at five sampling sites, differing in the level of soil contamination with risk elements. The findings showed high variability of the results regardless of the element determined, animal species, and sampling location. Among the elements, higher accumulation ability was observed for Cd and Zn, given the higher bioaccessibility of these elements in soils compared to Pb, Cr, and Cu. Higher Cd and Zn accumulation in the soft tissues of gastropods (without any statistically significant differences among the species) compared to C. populi was also recorded. Medians of the bioaccumulation factors (BAFs) reached up to 33.2 for Cd and 5.8 for Zn, in gastropods while reaching up to 3.4 for Cd, and 2.3 for Zn, for C. populi. For both groups of organisms, paradoxically, a higher rate of accumulation of risk elements was observed in all analyzed organisms in sites with lower soil contamination compared to heavily contaminated sites. This indicated the ability of the organisms living in extreme conditions to avoid the uptake of these elements or to move among areas of different contamination levels. Thus, terrestrial gastropods and C. populi proved to be unsuitable bioindicators for assessing soil pollution., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The North Bohemian Brown Coal Basin (Czech Republic) is suggested as a source of significant pollution in the surrounding environment with various pollutants, including risk elements. A total of 53 sampling points were selected within the North Bohemian region. The selected sampling points represented either the basin areas (affected by the coal mining and related activities) or the mountain areas (an area unaffected by the coal mining but characterized by the geogenic sources of the risk elements). At each of the sampling points, soils and respective dominant indigenous plant samples were collected. A suite of ecological indices, namely, individual pollution index (Ii), Nemerow index (PN), bioaccumulation factor (BAF), translocation factor (TF), and hazard quotient (HQ), were applied to estimate the environmental risk of As, Be, Cd, and Zn levels in soils, potential soil-plant transfer, and soil-plant-animal transport of these stated elements. The results from Ii showed that the maximum values of As, Be, Cd, and Zn in the investigated soils exceeded the preventive values, where the Ii value was up to 58 for As in the mountain areas, indicating severe pollution. At the same time, mild pollution was recorded in the case of Cd. For Be in the researched soils, its Ii assessment result was a wide range, varying between a clean environment and severe pollution. Whereas As and Be uptake by plants was limited and these elements were retained in the plant's roots, relatively high mobility and soil to plant shoots transport ability of Cd were recorded and documented by the TF values. The HQs calculated for selected herbivorous mammals in the area showed that the potential health risk of As and Be was limited to only plant roots in the hotspots with extreme As and Be contents. In comparison, substantial health risk of Cd was observed in the aboveground biomass of plants. Therefore, the potential remediation of the coal mining areas should be focused on (i) identification of the As and Be hotspots and (ii) to reduce the mobility and plant availability of Cd in the whole investigated area., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Objective: Body image disturbances and the attendant negative emotions are two of the major clinical symptoms of eating disorders. The objective of the present experimental study was to shed more light on the degree of association or dissociation between the physiological and emotional response to mirror exposure in patients with restrictive mental anorexia, and on the relationships between the physiological response and characteristics connected with emotional processing. Materials and Methods: Thirty adolescent girls with the restrictive type of anorexia and thirty matched healthy controls underwent bilateral measurement of skin conductance (SC) during rest, neutral stimulus exposure, and mirror exposure, and completed a set of measures focused on emotion regulation competencies, affectivity, and eating disorder pathology. Results: Compared to healthy controls, girls with restrictive anorexia rated mirror exposure as a subjectively more distressful experience. Differences in skin conductance response (SCR) were not significant; however, variance in SCR was substantially greater in the group of anorexia patients as compared to healthy controls. The overall skin conductance level (SCL) was lower in anorexia patients. Increase in SCR during mirror exposure, as opposed to exposure to neutral stimuli, was positively related to the tendency to experience negative emotions, interoceptive sensitivity, body dissatisfaction and suppression, but not to other symptoms of eating pathology or emotional awareness. A post hoc analysis suggested that physiological reactivity might be associated with interoceptive sensitivity to mirror exposure especially in anorectic patients. Conclusion: The study seems to demonstrate some degree of dissociation between psychophysiological reactivity and subjective response to body exposure in patients with restrictive anorexia. Factors affecting differences in psychophysiological responsiveness to body exposure in anorectic patients require further exploration., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Knejzlíková, Světlák, Malatincová, Roman, Chládek, Najmanová, Theiner, Linhartová and Kašpárek.)

The city of Litvínov (North Bohemia, Czech Republic) is seriously affected by coal mining, coal processing, and intensive industrial activities. Within the urban area, the potential environmental hazard of risk elements (in soil and vegetation) and polycyclic aromatic hydrocarbons (PAHs, in soils) was estimated using selected environmental and human health hazard indices. In total, 24 sites were sampled, including the city center, residential areas, industrialized zone, and areas close to operating and/or abandoned coal mines. The results showed elevated values of As, Cd, Cu, Ni, Pb, and Zn in soils (the maximum levels of individual pollution indices varied between 3 and 5 for As, Pb, and Zn); the risk assessment code (RAC) values indicated high bioaccessibility of Cd and Zn. The high mobility of Cd was confirmed by their bioaccumulation factors (BAF) in the aboveground biomass of Taraxacum sect. Ruderalia and Polygonum aviculare, reaching up to 1.9 and 2.9, respectively. The Cd content in plants presents a substantial health hazard for herbivores such as Oryctolagus cuniculus living within the urban area. The PAH levels in the soils also showed elevated values; the contents of benzo(a)pyrene exceeded more than 2-fold the indicative values for potential health risk for biota, especially near the abandoned coal mines. The incremental lifetime cancer risks (ILCR) for ingestion of the contaminated soil showed only low or negligible cancerogenic risk, varying between 6.7 × 10 -7 and 1.6 × 10 -5 for children, and between 9.9 × 10 -7 and 2.7 × 10 -6 for adults. However, the potential health impact of the inhalation of the contaminated particulate matter should be included in the further research. Although the contamination level in the investigated area does not represent an imminent environmental and health risk, the potential remediation measures should be considered in the future.

The potential environmental hazards of risk elements in the area affected by the opencast coal mine and/or coal combustion for plants and animals was assessed by using a suite of laboratory bioaccessibility tests. The chosen sampling area was in the vicinity of the largest coal mine spoil in the Sokolov coal basin (Czech Republic). For an estimation of the oral bioaccessibility of the risk elements in soils, the physiologically based extraction tests were applied. Among the available methods for estimating the pulmonary bioaccessibility of elements, the Gamble's and Hatch's tests were chosen. The results showed elevated pseudo-total soil contents of As, Be, Cd, Cu, Pb, V, and Zn. Among these elements, only Cd showed substantial bioaccessibility for plants, as documented by the high Risk Assessment Code, reaching up to 47%, and the highest plant-availability, where the maximum Bioaccumulation Factor in plants reached up to 4.5. The simulated body fluids showed the highest bioaccessibility of Cd, but also substantial bioaccessible pools of As and Be, the elements frequently found at the brown coal mining and processing areas. For better understanding of the risk element bioaccessibility under the specific conditions, the released element pools should be related to the particular soil physicochemical parameters.

Central Bohemia (Czech Republic) has highly developed industry and a dense rail network. Here, we aimed to determine the content of risk elements in dandelion plants (Taraxacum sect. Ruderalia) growing near train stations, industrial enterprises, and in the city parks of 16 cities in the Central Bohemian region. The highest element contents in the soils were found in industrial areas affected by the historical mining and smelting activities; contemporary industry showed no substantial effect on the soil element contents. The median values of element contents (As, Be, Cd, Co, Cr, Cu, Ni, Pb, and Zn) at the railway station sites were the highest among the monitored sites, where the differences between park and station sites were significant for Be, Co, and Zn. Although the intensity of the traffic at the individual stations differed, we found that long-term regular traffic enhanced the element contents in the soils and, subsequently, in the plants. For Cd, Co, Cr, Cu, Pb, V, and Zn, the highest median element contents were found in plant roots, regardless of the sampling site. For Cd and Zn, the contents in leaves were higher than in the inflorescences, and the opposite pattern was recorded for Co and Cu. As and Be were distributed equally among the plant parts. Among the sampling sites, the As, Be, Cd, Zn, and Pb contents in the plant roots tended to have higher median values at the station sites, confirming the results of our soil analyses. We detected a fairly good correlation between soil and plant content for cadmium, regardless of the sampling site, soil element content, or analyzed part of the plant. Thus, we propose that dandelion is a suitable bioindicator of cadmium pollution of soil.

Abandoned gold mines are often suggested as potential sources of environmental pollution. Thus, the soils within the area of a gold mine in Libčice, Czech Republic, were monitored. Elevated element contents were found of As, Cd, Cu, Hg, Pb, and Zn. The risk assessment codes (RACs) indicated high environmental risk from soil Cd, and moderate risk from Zn, whereas the risk of As, Cu, and Pb was low. It was supported by the analysis of 134 samples of aboveground biomass of plants, where the levels of As and Pb were below the detection limit. For Cd, the plant uptake reflected the high mobility of this element, where the bioaccumulation factors (BAFs) varied in range 0.032 (Fragaria vesca) and 1.97 (Circia arvensis). For 11% of samples the BAF values for Cd exceeded 1. For Hg, although the maximum BAF did not exceed 0.37 (Lotus corniculatus), the Hg contents in plants occasionally exceeded the threshold limits for Hg contents in raw feedstuffs. The investigated gold mine does not represent a direct environmental risk, but the fate of Cd and Hg in the soils and plants suggests the necessity of a deeper understanding of the penetration of these elements into the surrounding environment.

Three Cd and Zn hyperaccumulating plant species Noccaea caerulescens Noccaea praecox and Arabidopsis halleri (Brassicacceae) were cultivated in seven subsequent vegetation seasons in both pot and field conditions in soil highly contaminated with Cd, Pb, and Zn. The results confirmed the hyperaccumulation ability of both plant species, although A. halleri showed lower Cd uptake compared to N. caerulescens. Conversely, Pb phytoextraction was negligible for both species in this case. Because of the high variability in plant yield and element contents in the aboveground biomass of plants, great variation in Cd and Zn accumulation was observed during the experiment. The extraction ability in field conditions varied in the case of Cd from 0.2 to 2.9 kg ha(-1) (N. caerulescens) and up to 0.15 kg ha(-1) (A. halleri), and in the case of Zn from 0.2 to 6.4 kg ha(-1) (N. caerulescens) and up to 13.8 kg.ha(-1) (A. halleri). Taking into account the 20 cm root zone of the soil, the plants were able to extract up to 4.1% Cd and 0.2% Zn in one season. However, cropping measures should be optimized to improve and stabilize the long-term phytoextraction potential of these plants.

Total selenium (Se) contents were determined in aboveground biomass of wild plant species growing in two uncultivated meadows at two different locations. The soils in these locations had pseudototal (Aqua Regia soluble) Se in concentration ranges of between 0.2 and 0.3 mg kg(-1) at the first location, and between 0.7 and 1.4 mg kg(-1) at the second location. The plant species represented 29 plant families where the most numerous ones were Poaceae, Rosaceae, Fabaceae , and Asteraceae. The selenium contents in the plants varied between undetectable levels (Aegopodium podagraria, Achillea millefolium, Lotus corniculatus) and 0.158 mg kg(-1) (Veronica arvensis, Veronicaceae). The Se levels were roughly one order of magnitude lower compared to other elements with similar soil content, such as cadmium and molybdenum. The transfer factors of Se, quantifying the element transfer from soil to plants, varied between <0.001 and 0.146 with no significant differences between the locations, confirming the limited soil-plant selenium transfer regardless of location, soil Se level, and plant species. Among the plant families, no unambiguous trend to potential elevated Se uptake was observed. Low Se content in the soil and its plant availability was comparable to other Se-deficient areas within Europe.

A model small-scale field experiment was set up to investigate selenium (Se) uptake by four different varieties of broccoli plants, as well as the effect of Se foliar application on the uptake of essential elements for plants calcium (Ca), copper (Cu), iron (Fe), potassium (K), magnesium (Mg), manganese (Mn), phosphorus (P), sulfur (S), and zinc (Zn). Foliar application of sodium selenate (Na2SeO4) was carried out at two rates (25 and 50 g Se/ha), and an untreated control variant was included. Analyses of individual parts of broccoli were performed, whereby it was found that Se in the plant accumulates mainly in the flower heads and slightly less in the leaves, stems, and roots, regardless of the Se rate and broccoli variety. In most cases, there was a statistically significant increase of Se content in all parts of the plant, while there was no confirmed systematic influence of the addition of Se on the changing intake of other monitored elements. Selenization of broccoli leads to an effective increase in the Se content at a rate of 25 g/ha, whereas the higher rate did not result in a substantial increase of Se content compared to the lower rate in all varieties. Therefore, the rate of 25 g/ha can be recommended as effective to produce broccoli with an increased Se content suitable for consumption. Moreover, Se application resulted in an adequate increase of the main organic compounds of Se, such as selenocystine (SeCys2), selenomethionine (SeMet), and Se-methylselenocysteine (Se-MeSeCys).