13 results on '"Nho, Youn Hwa"'
Search Results
2. Lipid extract derived from newly isolated Rhodotorula toruloides LAB-07 for cosmetic applications
- Author
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Kim, Junyeob, Lee, Eun-Jung, Lee, Kyung-Eun, Nho, Youn-Hwa, Ryu, Jeoungjin, Kim, Su Young, Yoo, Jeong Kyun, Kang, Seunghyun, and Seo, Sang Woo
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- 2023
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3. Efficacy and safety of topical Streptococcus postbiotic emollient in adolescents and adults with mild‐to‐moderate atopic dermatitis: A randomized, double‐blind, vehicle‐controlled trial.
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Kim, Min Seo, Kim, Hyeon Jin, Kang, So Min, Heo, Young Mok, Kang, Jiseung, Ryu, Tae Kyeong, Kim, Hyun Jeong, Choi, Young‐Bong, Kim, Sol, Nho, Youn Hwa, Kang, Seunghyun, Smith, Lee, Koyanagi, Ai, Papadopoulos, Nikolaos G., Jo, Hyungwoo, Lee, Dong‐Geol, Shin, Jung U, and Yon, Dong Keon
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ATOPIC dermatitis ,ADULTS ,ECZEMA ,STREPTOCOCCUS ,TEENAGERS - Abstract
A study was conducted to evaluate the effectiveness and safety of a topical emollient in treating mild-to-moderate atopic dermatitis (AD) in adolescents and adults. The study involved 100 participants and lasted for 24 weeks. The results showed that the group using the emollient had significant improvements in various measures compared to the placebo group. No safety concerns were reported. However, the study had limitations and further research is needed to explore other aspects of AD and the effects of the emollient. Overall, the emollient shows promise as a therapeutic option for long-term care of AD patients. [Extracted from the article]
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- 2024
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4. A Glance into the Destiny of Transcriptomic Activity, Embodied by the HOX Genes, in Neonatal and Aging Dermal Cells.
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Ko, Doyeong, Mun, Seyoung, Kim, Minji, Nho, Youn‐Hwa, Lee, Dong‐Geol, Kang, Seunghyun, Han, Kyudong, and Kim, Misun
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HOMEOBOX genes ,CELLULAR aging ,SKIN aging ,GENE families ,GENE expression ,EMBRYOLOGY - Abstract
Skin is an organ having a crucial role in the protection of muscle, bone, and internal organs and undergoing continuous self‐renewal and aged. The growing interest in the prevention of skin aging and rejuvenation has sparked a surge of industrial and research studies focusing on the biological and transcriptional changes that occur during skin development and aging. In this study, the aim is to identify transcriptional differences between two main types of human skin cells: the human dermal fibroblasts (HDFs) and the human epidermis keratinocytes (HEKs) isolated from 30 neonatal and 30 adults (old) skin. Through differentially expressed gene (DEG) profiling using DEseq2, 604 up‐, and 769 down‐regulated genes are identified in the old group. A functional analysis using Metascape Gene Ontology and Reactome pathways revealed systematic transcriptomic shifts in key skin formation and maintenance markers, alongside a distinct difference in HOX gene families crucial for embryonic development and diverse biological processes. Among the 39 human HOX gene family, ten posterior HOX genes (HOXA10, 11, 13, HOXB13, HOXC11, and HOXD9‐13) are significantly downregulated, and anterior 25 genes (HOXA2‐7, HOXB1‐9, HOXC4‐6 and 8–9, and HOXD1,3,4 and 8) are upregulated, especially in the old HDFs. The study successfully demonstrates the correlation between HOX genes and the skin aging process, providing strong evidence that HOX genes are proposed as a new marker for skin aging assessment. [ABSTRACT FROM AUTHOR]
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- 2024
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5. Recombinant Lignin Peroxidase with Superior Thermal Stability and Melanin Decolorization Efficiency in a Typical Human Skin-Mimicking Environment.
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Gye, Hyeryeong, Baek, Heeyeon, Han, Seunghyun, Kwon, Haeun, Nguyen, Trang Vu Thien, Pham, Le Thanh Mai, Kang, Seunghyun, Nho, Youn Hwa, Lee, Dong Woog, and Kim, Yong Hwan
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- 2023
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6. Anti-melanogenic effects of resorcinol are mediated by suppression of cAMP signaling and activation of p38 MAPK signaling.
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Kang, Mingyeong, Park, See-Hyoung, Oh, Sae Woong, Lee, Seung Eun, Yoo, Ju Ah, Nho, Youn Hwa, Lee, Sukyeon, Han, Byung Seok, Cho, Jae Youl, and Lee, Jongsung
- Subjects
RESORCINOL ,MITOGEN-activated protein kinases ,MICROPHTHALMIA-associated transcription factor - Abstract
In this study, we investigated the inhibitory mechanisms of resorcinol in B16F10 mouse melanoma cells. We found that resorcinol reduced both the melanin content and tyrosinase activity in these cells. In addition, resorcinol suppressed the expression of melanogenic gene microphthalmia-associated transcriptional factor (MITF) and its downstream target genes tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2. In addition, we found that resorcinol reduced intracellular cAMP levels and protein kinase A (PKA) activity, and increased phosphorylation of the p38 mitogen-activated protein kinase (MAPK). Resorcinol was also found to directly inhibit tyrosinase activity. However, resorcinol-induced decrease in melanin content, tyrosinase activity, and tyrosinase protein levels were attenuated by SB203580, a p38 MAPK inhibitor. Taken together, these data indicate that anti-melanogenic activity of resorcinol is be mediated through the inhibition of cAMP signaling and activation of p38 MAPK, indicating that resorcinol may be a possible ameliorating agent in the treatment of hyperpigmentation skin disorders. [ABSTRACT FROM AUTHOR]
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- 2018
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7. Caviar Extract and Its Constituent DHA Inhibits UVB-Irradiated Skin Aging by Inducing Adiponectin Production.
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Lee, Kyung-Eun, Nho, Youn-Hwa, Yun, Seok Kyun, Park, Sung-Min, Kang, Seunghyun, and Yeo, Hyeonju
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SKIN aging , *ADIPONECTIN , *ADIPOGENESIS , *DOCOSAHEXAENOIC acid , *TRANSCRIPTION factors , *EXTRACTS , *AGING prevention - Abstract
In this study, caviar (sturgeon eggs) was used to elucidate its roles in adiponectin production and skin anti-aging. Recently, caviar has been largely used not only as a nutritional food, but also in cosmetic products. In particular, it has been reported that docosahexaenoic acid (DHA), as one of the main phospholipid components of caviar extract, induces intracellular lipid accumulation and the expression of adiponectin in adipocytes. Although adipocytes are well known to be associated with the skin dermis by secreting various factors (e.g., adiponectin), the effects of caviar extract and DHA on the skin are not well studied. Here, we demonstrate the effects of caviar extract and DHA on adipocyte differentiation and adiponectin production, resulting in a preventive role in UV-irradiated skin aging. Caviar extract and DHA enhanced adipocyte differentiation and promoted the synthesis of transcription factors controlling adipocyte differentiation and adiponectin. In addition, the mRNA expression levels of matrix metalloproteinase-1 (MMP-1) were decreased in UVB-irradiated Hs68 fibroblasts that were cultured in conditioned medium from caviar extract or DHA-treated differentiated adipocytes. Taken together, these results indicate that caviar extract and DHA induce adipocyte differentiation and adiponectin production, thereby inhibiting UVB-induced premature skin aging via the suppression of MMP-1 production. [ABSTRACT FROM AUTHOR]
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- 2020
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8. Emodin isolated from Polygoni Multiflori Ramulus inhibits melanogenesis through the liver X receptor-mediated pathway.
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Kim, Mi Ok, Park, Yong Seek, Nho, Youn Hwa, Yun, Seok Kyun, Kim, Youngsoo, Jung, Eunsun, Paik, Jean Kyung, Kim, Minhee, Cho, Il-Hoon, and Lee, Jongsung
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MELANOGENESIS , *EMODIN , *MELANINS , *LEGUMES , *PLANT extracts , *NUCLEAR receptors (Biochemistry) - Abstract
Melanogenesis is a physiological process that results in the synthesis of melanin pigments, which play a crucial protective role against skin photocarcinogenesis. We investigated the effects of a Polygoni Multiflori Ramulus extract on melanogenesis and isolated emodin from Polygoni Multiflori as an active compound. In addition, the possible mechanisms of action were examined. We found that emodin inhibited both melanin content and tyrosinase activity concentration and time dependently. Tyrosinase, tyrosinase-related protein (TRP)-1, and TRP-2 mRNA levels decreased following emodin treatment. However, while the mRNA levels of microphthalmia-associated transcription factor (MITF) were not affected by emodin, emodin reduced MITF protein levels. Furthermore, expression of the liver X-receptor (LXR) α gene, but not the LXR β gene was upregulated by emodin. Moreover, emodin regulated melanogenesis by promoting degradation of the MITF protein by upregulating the LXR α gene. The emodin effects on MITF was found to be mediated by phosphorylation of p42/44 MAPK. Taken together, these findings indicate that the inhibition of melanogenesis by emodin occurs through reduced MITF protein expression, which is mediated by upregulation of the LXR α gene and suggest that emodin may be useful as a hyperpigmentation inhibitor. [ABSTRACT FROM AUTHOR]
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- 2016
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9. Cannabidiol upregulates melanogenesis through CB1 dependent pathway by activating p38 MAPK and p42/44 MAPK.
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Hwang, Young Sun, Kim, Youn-Jung, Kim, Mi Ok, Kang, Mingyeong, Oh, Sae Woong, Nho, Youn Hwa, Park, See-Hyoung, and Lee, Jongsung
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MELANOGENESIS , *ULTRAVIOLET radiation , *MESSENGER RNA , *PHENOL oxidase , *CYCLIC adenylic acid - Abstract
Melanogenesis plays a critical role in the protection of skin against external stresses such as ultraviolet irradiation and oxidative stressors. This study was aimed to investigate the effects of cannabidiol on melanogenesis and its mechanisms of action in human epidermal melanocytes. We found that cannabidiol increased both melanin content and tyrosinase activity. The mRNA levels of microphthalmia-associated transcription factor (MITF), tyrosinase, tyrosinase-related protein (TRP) 1, and TRP2 were increased following cannabidiol treatment. Likewise, cannabidiol increased the protein levels of MITF, TRP 1, TRP 2, and tyrosinase. Mechanistically, we found that cannabidiol regulated melanogenesis by upregulating MITF through phosphorylation of p38 mitogen-activated protein kinase (MAPK) and p42/44 MAPK, independent of cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) signaling. In addition, the melanogenic effect of cannabidiol was found to be mediated by cannabinoid CB 1 receptor, not by CB 2 receptor. Taken together, these findings indicate that cannabidiol-induced melanogenesis is cannabinoid CB 1 receptor-dependent, and cannabidiol induces melanogenesis through increasing MITF gene expression which is mediated by activation of p38 MAPK and p42/44 MAPK. Our results suggest that cannabidiol might be useful as a protective agent against external stresses. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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10. Afzelin positively regulates melanogenesis through the p38 MAPK pathway.
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Jung, Eunsun, Kim, Jin Hee, Kim, Mi Ok, Jang, Sunghee, Kang, Mingyeong, Oh, Sae Woong, Nho, Youn Hwa, Kang, Seung Hyun, Kim, Min Hee, Park, See-Hyoung, and Lee, Jongsung
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MELANOGENESIS , *MITOGEN-activated protein kinases , *IRRADIATION , *OXIDATIVE stress , *MICROPHTHALMIA-associated transcription factor , *TYRP1 gene , *CYCLIC adenylic acid , *GENE expression - Abstract
Melanogenesis refers to synthesis of the skin pigment melanin, which plays a critical role in the protection of skin against ultraviolet irradiation and oxidative stressors. We investigated the effects of afzelin on melanogenesis and its mechanisms of action in human epidermal melanocytes. In this study, we found that afzelin increased both melanin content and tyrosinase activity in a concentration-dependent manner. While the mRNA levels of microphthalmia-associated transcription factor (MITF), tyrosinase, and tyrosinase-related protein (TRP)-1 increased following afzelin treatment, the mRNA levels of TRP-2 were not affected by afzelin. Likewise, afzelin increased the protein levels of MITF, TRP-1, and tyrosinase but not TRP-2. Mechanistically, we found that afzelin regulated melanogenesis by upregulating MITF through phosphorylation of p38 mitogen-activated protein kinase (MAPK), independent of cyclic adenosine monophosphate (cAMP)-protein kinase A (PKA) signaling. Taken together, these findings indicate that the promotion of melanogenesis by afzelin occurs through increased MITF gene expression, which is mediated by activation of p38 MAPK, and suggest that afzelin may be useful as a protective agent against ultraviolet irradiation. [ABSTRACT FROM AUTHOR]
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- 2016
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11. Anti-invasive and Anti-tumor Effects of Dryopteris crassirhizoma Extract by Disturbing Actin Polymerization.
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Lee J, Nho YH, Yun SK, and Hwang YS
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- Animals, Carcinoma, Squamous Cell metabolism, Cell Line, Tumor, Cell Proliferation drug effects, Humans, Male, Mice, Mice, Inbred BALB C, Phosphorylation drug effects, Actins metabolism, Antineoplastic Agents pharmacology, Carcinoma, Squamous Cell drug therapy, Dryopteris chemistry, Neoplasm Invasiveness prevention & control, Plant Extracts pharmacology, Polymerization drug effects
- Abstract
Aim: To evaluate the anti-invasive effect of ethanol extracts of rhizome of Dryopteris crassirhizoma (EEDC) in matrix invasion and formation of functional invadopodia and to determine the anti-tumor effect of EEDC in a mouse model of mandibular invasion by gingival squamous cell carcinoma (SCC)., Methods: The rhizome of D crassirhizoma was extracted in ethanol. The anti-invasive effect of EEDC was analyzed with a Matrigel-coated transwell invasion and 3D culture system. Crucial factors related to the control of cancer cell invasion by EEDC were determined using a human protease array. Molecular evidence supporting the anti-invasive effect of EEDC in oral SCC (OSCC) cells used an invadopodia-mediated extracellular matrix (ECM) degradation; an in vivo athymic mouse model was also provided., Results: EEDC treatment (10 µg/mL) suppressed transwell migration and invasion of HSC-3 OSCC cells without cytotoxicity. Decreased levels of matrix metalloprotease (MMP)-7, kalikrein 10, cathepsin V, MMP-2, and cathepsin D were also found in EEDC-treated HSC-3 cells based on human protease array. The anti-invasive effects of EEDC involved the suppression of invadopodia-mediated ECM degradation via inhibition of globular-actin elongation. The anti-invasive effect resulting from disturbance of functional invadopodia formation by EEDC was observed even at a low concentration of 5 µg/mL. The phosphorylation of cortactin involved in functional invadopodia formation was decreased at EEDC concentrations that inhibited invadopodia formation. The anti-tumor effect of EEDC was also observed in a mouse xenograft model. Administration of EEDC resulted in inhibition of tumor growth and progression., Conclusions: EEDC represents a potential anti-invasive and anti-tumor agent in cancer control.
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- 2019
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12. Use of ethanol extracts of Terminalia chebula to prevent periodontal disease induced by dental plaque bacteria.
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Lee J, Nho YH, Yun SK, and Hwang YS
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- Animals, Anti-Bacterial Agents therapeutic use, Anti-Inflammatory Agents therapeutic use, Bacteria growth & development, Bone Resorption microbiology, Bone Resorption prevention & control, Cyclooxygenase 2 metabolism, Cytokines metabolism, Dinoprostone metabolism, Gingivitis microbiology, Gingivitis prevention & control, Inflammation microbiology, Inflammation prevention & control, Mice, Mouth microbiology, Osteoblasts drug effects, Osteoclasts drug effects, Peptide Hydrolases metabolism, Periodontitis microbiology, Periodontitis prevention & control, Phytotherapy, Plant Extracts therapeutic use, RANK Ligand metabolism, RAW 264.7 Cells, Anti-Bacterial Agents pharmacology, Anti-Inflammatory Agents pharmacology, Bacteria drug effects, Dental Plaque microbiology, Periodontal Diseases microbiology, Periodontal Diseases pathology, Periodontal Diseases prevention & control, Plant Extracts pharmacology, Terminalia
- Abstract
Background: The fruit of the Terminalia chebula tree has been widely used for the treatment of various disorders. Its anti-diabetic, anti-mutagenic, anti-oxidant, anti-bacterial, anti-fungal, and anti-viral effects have been studied. Dental plaque bacteria (DPB) are intimately associated with gingivitis and periodontitis. In the quest for materials that will prove useful in the treatment and prevention of periodontal disease, we investigated the preventive effects of an ethanol extract of Terminalia chebula (EETC) on DPB-induced inflammation and bone resorption., Methods: The anti-bacterial effect of EETC was analyzed using the disc diffusion method. The anti-inflammatory effect of EETC was determined by molecular biological analysis of the DPB-mediated culture cells. Prevention of osteoclastic bone resorption by EETC was explored using osteoclast formation and pit formation assays., Results: EETC suppressed the growth of oral bacteria and reduced the induction of inflammatory cytokines and proteases, abolishing the expression of PGE2 and COX-2 and inhibiting matrix damage. By stimulating the DPB-derived lipopolysaccharides, EETC inhibited both osteoclast formation in osteoclast precursors and RANKL expression in osteoblasts, thereby contributing to the prevention of bone resorption., Conclusions: EETC may be a beneficial supplement to help prevent DPB-mediated periodontal disease.
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- 2017
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13. A Mixture of Extracts of Kochia scoparia and Rosa multiflora with PPAR α/γ Dual Agonistic Effects Prevents Photoaging in Hairless Mice.
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Jeon H, Kim DH, Nho YH, Park JE, Kim SN, and Choi EH
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- Animals, Female, Gene Expression Regulation drug effects, Gene Expression Regulation radiation effects, Matrix Metalloproteinase 1 analysis, Matrix Metalloproteinase 1 genetics, Matrix Metalloproteinase 13 genetics, Mice, Hairless, PPAR alpha genetics, PPAR gamma genetics, Plant Extracts chemistry, Procollagen genetics, Skin drug effects, Skin metabolism, Skin radiation effects, Skin ultrastructure, Transforming Growth Factor beta genetics, Ultraviolet Rays adverse effects, Bassia scoparia chemistry, PPAR alpha agonists, PPAR gamma agonists, Plant Extracts pharmacology, Rosa chemistry, Skin Aging drug effects, Skin Aging radiation effects
- Abstract
Activation of peroxisome proliferator-activated receptors (PPAR) α/γ is known to inhibit the increases in matrix metalloproteinase (MMP) and reactive oxygen species (ROS) induced by ultraviolet light (UV). Extracts of natural herbs, such as Kochia scoparia and Rosa multiflora , have a PPAR α/γ dual agonistic effect. Therefore, we investigated whether and how they have an antiaging effect on photoaging skin. Eighteen-week-old hairless mice were irradiated with UVA 14 J/cm² and UVB 40 mJ/cm² three times a week for 8 weeks. A mixture of extracts of Kochia scoparia and Rosa multiflora (KR) was topically applied on the dorsal skin of photoaging mice twice a day for 8 weeks. Tesaglitazar, a known PPAR α/γ agonist, and vehicle (propylene glycol:ethanol = 7:3, v / v ) were applied as positive and negative controls, respectively. Dermal effects (including dermal thickness, collagen density, dermal expression of procollagen 1 and collagenase 13) and epidermal effects (including skin barrier function, epidermal proliferation, epidermal differentiation, and epidermal cytokines) were measured and compared. In photoaging murine skin, KR resulted in a significant recovery of dermal thickness as well as dermal fibroblasts, although it did not change dermal collagen density. KR increased the expression of dermal transforming growth factor (TGF)-β. The dermal effects of KR were explained by an increase in procollagen 1 expression, induced by TGF-β, and a decrease in MMP-13 expression. KR did not affect basal transepidermal water loss (TEWL) or stratum corneum (SC) integrity, but did decrease SC hydration. It also did not affect epidermal proliferation or epidermal differentiation. KR decreased the expression of epidermal interleukin (IL)-1α. Collectively, KR showed possible utility as a therapeutic agent for photoaging skin, with few epidermal side effects such as epidermal hyperplasia or poor differentiation., Competing Interests: The authors declare no conflict of interest.
- Published
- 2016
- Full Text
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