10 results on '"Ojima, Miriam N"'
Search Results
2. A bacterial sulfoglycosidase highlights mucin O-glycan breakdown in the gut ecosystem
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Katoh, Toshihiko, Yamada, Chihaya, Wallace, Michael D., Yoshida, Ayako, Gotoh, Aina, Arai, Moe, Maeshibu, Takako, Kashima, Toma, Hagenbeek, Arno, Ojima, Miriam N., Takada, Hiromi, Sakanaka, Mikiyasu, Shimizu, Hidenori, Nishiyama, Keita, Ashida, Hisashi, Hirose, Junko, Suarez-Diez, Maria, Nishiyama, Makoto, Kimura, Ikuo, Stubbs, Keith A., Fushinobu, Shinya, and Katayama, Takane
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- 2023
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3. Priority effects shape the structure of infant-type Bifidobacterium communities on human milk oligosaccharides
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Ojima, Miriam N., Jiang, Lin, Arzamasov, Aleksandr A., Yoshida, Keisuke, Odamaki, Toshitaka, Xiao, Jinzhong, Nakajima, Aruto, Kitaoka, Motomitsu, Hirose, Junko, Urashima, Tadasu, Katoh, Toshihiko, Gotoh, Aina, van Sinderen, Douwe, Rodionov, Dmitry A., Osterman, Andrei L., Sakanaka, Mikiyasu, and Katayama, Takane
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- 2022
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4. Human Milk Oligosaccharide Utilization in Intestinal Bifidobacteria Is Governed by Global Transcriptional Regulator NagR.
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Arzamasov, Aleksandr A., Aruto Nakajima, Mikiyasu Sakanak, Ojima, Miriam N., Takane Katayama, Rodionov, Dmitry A., and Osterman, Andrei L.
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- 2022
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5. Ecological and molecular perspectives on responders and non-responders to probiotics and prebiotics.
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Ojima, Miriam N, Yoshida, Keisuke, Sakanaka, Mikiyasu, Jiang, Lin, Odamaki, Toshitaka, and Katayama, Takane
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PREBIOTICS , *PROBIOTICS , *GUT microbiome , *INFANT formulas , *BREAST milk , *COLONIZATION (Ecology) , *SYNBIOTICS - Abstract
[Display omitted] • Responder-status to probiotics and prebiotics vary among individuals. • Niche-based processes can predict probiotic colonization success in the microbiota. • Response to prebiotics is predictable by key enzyme gene abundance in the microbiota. • Interventions for the gut microbiota require both ecological and genetic perspectives. Bifidobacteria are widely used as a probiotic for their health-promoting effects. To promote their growth, bifidogenic prebiotics, including human milk oligosaccharides (HMOs), have been added to supplements and infant formula. However, the efficacy of both probiotic and prebiotic interventions is often debated, as clinical responses vary significantly by case. Here, we review clinical studies that aimed to proliferate human-residential Bifidobacterium (HRB) strains in the gut, and we highlight the difference between responders and non-responders to such interventions through an ecological, niche-based perspective and an examination of the prevalence of genes responsible for prebiotic assimilation in HRB genomes. We discuss the criteria necessary to better evaluate the efficacy of probiotic and prebiotic interventions and the recent therapeutic potential shown by synbiotics. [ABSTRACT FROM AUTHOR]
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- 2022
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6. Diversification of a Fucosyllactose Transporter within the Genus Bifidobacterium.
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Ojima, Miriam N., Yuya Asao, Aruto Nakajima, Toshihiko Katoh, Motomitsu Kitaoka, Aina Gotoh, Junko Hirose, Tadasu Urashima, Satoru Fukiya, Atsushi Yokota, Hachem, Maher Abou, Mikiyasu Sakanaka, and Takane Katayama
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BIFIDOBACTERIUM , *BREAST milk , *HEALTH maintenance organizations , *GENOMICS , *GENE expression , *PHYSIOLOGICAL adaptation - Abstract
Human milk oligosaccharides (HMOs), which are natural bifidogenic prebiotics, were recently commercialized to fortify formula milk. However, HMO assimilation phenotypes of bifidobacteria vary by species and strain, which has not been fully linked to strain genotype. We have recently shown that specialized uptake systems, particularly for the internalization of major HMOs (fucosyllactose [FL]), are associated with the formation of a Bifidobacterium-rich gut microbial community. Phylogenetic analysis revealed that FL transporters have diversified into two clades harboring four clusters within the Bifidobacterium genus, but the underpinning functional diversity associated with this divergence remains underexplored. In this study, we examined the HMO consumption phenotypes of two bifidobacterial species, Bifidobacterium catenulatum subsp. kashiwanohense and Bifidobacterium pseudocatenulatum, both of which possess FL-binding proteins that belong to phylogenetic clusters with unknown specificities. Growth assays, heterologous gene expression experiments, and HMO consumption analyses showed that the FL transporter type from B. catenulatum subsp. kashiwanohense JCM 15439T conferred a novel HMO uptake pattern that includes complex fucosylated HMOs (lacto-N-fucopentaose II and lacto-N-difucohexaose I/II). Further genomic landscape analyses of FL transporterpositive bifidobacterial strains revealed that the H-antigen- or Lewis antigen-specific fucosidase gene(s) and FL transporter specificities were largely aligned. These results suggest that bifidobacteria have acquired FL transporters along with the corresponding gene sets necessary to utilize the imported HMOs. Our results provide insight into the species- and strain-dependent adaptation strategies of bifidobacteria in HMO-rich environments. [ABSTRACT FROM AUTHOR]
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- 2022
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7. Bifidobacterium bifidum Suppresses Gut Inflammation Caused by Repeated Antibiotic Disturbance Without Recovering Gut Microbiome Diversity in Mice.
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Ojima, Miriam N., Gotoh, Aina, Takada, Hiromi, Odamaki, Toshitaka, Xiao, Jin-Zhong, Katoh, Toshihiko, and Katayama, Takane
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GUT microbiome ,BIFIDOBACTERIUM bifidum ,RIBOSOMAL RNA ,FECAL microbiota transplantation ,ANTIBIOTICS ,TREATMENT effectiveness ,PROTEOBACTERIA - Abstract
The gut microbiome is a dynamic community that significantly affects host health; it is frequently disturbed by medications such as antibiotics. Recently, probiotics have been proposed as a remedy for antibiotic-induced dysbiosis, but the efficacy of such treatments remains uncertain. Thus, the effect of specific antibiotic-probiotic combinations on the gut microbiome and host health warrants further research. We tested the effect vancomycin, amoxicillin, and ciprofloxacin on mice. Antibiotic administration was followed by one of the following recovery treatments: Bifidobacterium bifidum JCM 1254 as a probiotic (PR); fecal transplant (FT); or natural recovery (NR). Each antibiotic administration and recovery treatment was repeated three times over 9 weeks. We used the Shannon Index and Chao1 Index to determine gut microbiome diversity and assessed recovery by quantifying the magnitude of microbial shift using the Bray-Curtis Index of Dissimilarity. We determined the community composition by sequencing the V3–V4 regions of the 16S ribosomal RNA gene. To assess host health, we measured body weight and cecum weight, as well as mRNA expression of inflammation-related genes by reverse-transcription quantitative PCR. Our results show that community response varied by the type of antibiotic used, with vancomycin having the most significant effects. As a result, the effect of probiotics and fecal transplants also varied by antibiotic type. For vancomycin, the first antibiotic disturbance substantially increased the relative abundance of inflammatory species in the phylum Proteobacteria, such as Proteus , but the effect of subsequent disturbances was less pronounced, suggesting that the gut microbiome is affected by past disturbance events. Furthermore, although gut microbiome diversity did not recover, probiotic supplementation was effective in limiting cecum size enlargement and colonic inflammation caused by vancomycin. However, for amoxicillin and ciprofloxacin, the relative abundances of proinflammatory species were not greatly affected, and consequently, the effect of probiotic supplementation on community structure, cecum weight, and expression of inflammation-related genes was comparatively negligible. These results indicate that probiotic supplementation is effective, but only when antibiotics cause proinflammatory species-induced gut inflammation, suggesting that the necessity of probiotic supplementation is strongly influenced by the type of disturbance introduced to the community. [ABSTRACT FROM AUTHOR]
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- 2020
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8. Interactive effects of disturbance and dispersal on community assembly.
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Ojima, Miriam N. and Jiang, Lin
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PROTISTA , *DISPERSAL (Ecology) , *BIOLOGICAL divergence , *BIOTIC communities , *DISPERSAL of microorganisms - Abstract
The traditional debate on alternative community states has been over whether or not they exist. Studies of community assembly have examined the role of assembly history in driving community divergence, but the context in which assembly history becomes important is a continued topic of interest. In this study, we created communities of bacterivorous ciliated protists in laboratory microcosms and manipulated assembly history, disturbance frequency, and the presence of dispersal among local communities to investigate the mechanisms behind community divergence. Specifically, we sought to understand how the role of assembly history changed in response to disturbance, dispersal, and the combination of the two. Assembly history influenced the identity of the dominant species through priority effects, and dispersal and disturbance showed interactive effects on both alpha and beta diversity. Dispersal substantially increased alpha diversity, but only in the absence of disturbance, and it reduced beta diversity, but not in the presence of low or mixed disturbance. These results demonstrate that the role of assembly history and the strength of priority effects depend on community context, suggesting that understanding the interactions between various factors shaping community assembly is important for understanding how ecological communities are structured. [ABSTRACT FROM AUTHOR]
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- 2017
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9. Enzymatic Adaptation of Bifidobacterium bifidum to Host Glycans, Viewed from Glycoside Hydrolyases and Carbohydrate-Binding Modules.
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Katoh, Toshihiko, Ojima, Miriam N., Sakanaka, Mikiyasu, Ashida, Hisashi, Gotoh, Aina, and Katayama, Takane
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BIFIDOBACTERIUM bifidum ,GLYCANS ,GLYCOSIDASES ,BREAST milk ,ALTRUISM ,OLIGOSACCHARIDES ,SOMATOTROPIN receptors - Abstract
Certain species of the genus Bifidobacterium represent human symbionts. Many studies have shown that the establishment of symbiosis with such bifidobacterial species confers various beneficial effects on human health. Among the more than ten (sub)species of human gut-associated Bifidobacterium that have significantly varied genetic characteristics at the species level, Bifidobacterium bifidum is unique in that it is found in the intestines of a wide age group, ranging from infants to adults. This species is likely to have adapted to efficiently degrade host-derived carbohydrate chains, such as human milk oligosaccharides (HMOs) and mucin O-glycans, which enabled the longitudinal colonization of intestines. The ability of this species to assimilate various host glycans can be attributed to the possession of an adequate set of extracellular glycoside hydrolases (GHs). Importantly, the polypeptides of those glycosidases frequently contain carbohydrate-binding modules (CBMs) with deduced affinities to the target glycans, which is also a distinct characteristic of this species among members of human gut-associated bifidobacteria. This review firstly describes the prevalence and distribution of B. bifidum in the human gut and then explains the enzymatic machinery that B. bifidum has developed for host glycan degradation by referring to the functions of GHs and CBMs. Finally, we show the data of co-culture experiments using host-derived glycans as carbon sources, which underpin the interesting altruistic behavior of this species as a cross-feeder. [ABSTRACT FROM AUTHOR]
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- 2020
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10. A simple method that enhances minority species detection in the microbiota: 16S metagenome-DRIP (Deeper Resolution using an Inhibitory Primer).
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Nakajima A, Yoshida K, Gotoh A, Katoh T, Ojima MN, Sakanaka M, Xiao JZ, Odamaki T, and Katayama T
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Aim: 16S rRNA gene-based microbiota analyses (16S metagenomes) using next-generation sequencing (NGS) technologies are widely used to examine the microbial community composition in environmental samples. However, the sequencing capacity of NGS is sometimes insufficient to cover the whole microbial community, especially when analyzing soil and fecal microbiotas. This limitation may have hampered the detection of minority species that potentially affect microbiota formation and structure. Methods: We developed a simple method, termed 16S metagenome-DRIP (Deeper Resolution using an Inhibitory Primer), that not only enhances minority species detection but also increases the accuracy of their abundance estimation. The method relies on the inhibition of normal amplicon formation of the 16S rRNA gene of a target major (abundant) species during the first PCR step. The addition of a biotinylated primer that is complementary to the variable sequence of the V3-V4 region of the target species inhibits a normal amplification process to form an aberrant short amplicon. The fragment is then captured by streptavidin beads for removal from the reaction mixture, and the resulting mixture is utilized for the second PCR with barcode-tag primers. Thus, this method only requires two additional experimental procedures to the conventional 16S metagenome analysis. A proof-of-concept experiment was first conducted using a mock sample consisting of the genomes of 14 bacterial species. Then, the method was applied to infant fecal samples using a Bifidobacterium -specific inhibitory primer ( n = 11). Results: As a result, the reads assigned to the family Bifidobacteriaceae decreased on average from 16,657 to 1718 per sample without affecting the total read counts (36,073 and 34,778 per sample for the conventional and DRIP methods, respectively). Furthermore, the minority species detection rate increased with neither affecting Bray-Curtis dissimilarity calculated by omitting the target Bifidobacterium species (median: 0.049) nor changing the relative abundances of the non-target species. While 115 amplicon sequence variants (ASVs) were unique to the conventional method, 208 ASVs were uniquely detected for the DRIP method. Moreover, the abundance estimation for minority species became more accurate, as revealed thorough comparison with the results of quantitative PCR analysis. Conclusion: The 16S metagenome-DRIP method serves as a useful technique to grasp a deeper and more accurate microbiota composition when combined with conventional 16S metagenome analysis methods., Competing Interests: Employment of Ojima MN and Sakanaka M at Kyoto University is supported by Morinaga Milk Industry Co., Ltd. Yoshida K, Xiao JZ, and Odamaki T are employees of Morinaga Milk Industry Co., Ltd. The authors declared that there are no other conflicts of interest., (© The Author(s) 2022.)
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- 2022
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