Search

Your search keyword '"Oliveira, Valeria M."' showing total 9 results
Start Over Author "Oliveira, Valeria M." Publication Type Academic Journals
9 results on '"Oliveira, Valeria M."'

9. Monitoring the bacterial community dynamics in a petroleum refinery wastewater membrane bioreactor fed with a high phenolic load.

Author

Silva CC, Viero AF, Dias AC, Andreote FD, Jesus EC, De Paula SO, Torres AP, Santiago VM, and Oliveira VM

Subjects
Bacteria classification, Bacteria genetics, Bacteria metabolism, DNA, Bacterial genetics, DNA, Ribosomal genetics, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S genetics, Thauera classification, Thauera genetics, Thauera isolation & purification, Thauera metabolism, Bacteria isolation & purification, Biodiversity, Bioreactors microbiology, Industrial Waste analysis, Petroleum analysis, Phenols metabolism
Abstract

The phenolic compounds are a major contaminant class often found in industrial wastewaters and the biological treatment is an alternative tool commonly employed for their removal. In this sense, monitoring microbial community dynamics is crucial for a successful wastewater treatment. This work aimed to monitor the structure and activity of the bacterial community during the operation of a laboratory-scale continuous submerged membrane bioreactor (SMBR), using PCR and RT-PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE) and 16S rRNA libraries. Multivariate analyses carried out using DGGE profiles showed significant changes in the total and metabolically active dominant community members during the 4-week treatment period, explained mainly by phenol and ammonium input. Gene libraries were assembled using 16S rDNA and 16S rRNA PCR products from the fourth week of treatment. Sequencing and phylogenetic analyses of clones from 16S rDNA library revealed a high diversity of taxa for the total bacterial community, with predominance of Thauera genus (ca. 50%). On the other hand, a lower diversity was found for metabolically active bacteria, which were mostly represented by members of Betaproteobacteria (Thauera and Comamonas), suggesting that these groups have a relevant role in the phenol degradation during the final phase of the SMBR operation.

Published
2010

Catalog

Books, media, physical & digital resources
See catalog results