18 results on '"Orencole, S."'
Search Results
2. Chlorpromazine specifically inhibits peripheral and brain TNF production, and up-regulates IL-10 production, in mice.
- Author
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Mengozzi, M., Fantuzzi, G., Faggioni, R., Marchant, A., Goldman, M., Orencole, S., Clark, B. D., Sironi, M., Benigni, F., and Ghezzi, P.
- Subjects
CHLORPROMAZINE ,IMMUNOGLOBULINS ,ENDOTOXINS ,TUMOR necrosis factors ,MONOCLONAL antibodies ,MICE - Abstract
We have previously shown that chlorpromazine (CPZ) inhibits tumour necrosis factor (TNF) production and protects against endotoxic shock in mice. In this paper we investigated the effect of pretreatment with CPZ, 4 mg/kg i.p. 30 min before, compared with dexamethasone (DEX; 3 mg/ kg) on the induction of other endotoxin (lipopolysaccharide; LPS)-induced cytokines in the serum of mice, i.e. interleukin-lα (IL-lα), IL-6 and IL-b, and TNF. We also studied the effect of CPZ on serum and spleen-associated TNF. Both DEX and CPZ inhibited TNF production, whereas induction of IL-b and IL-6 was inhibited by DEX but not by CPZ. DEX did not affect IL-l0, while CPZ potentiated its induction. CPZ also inhibited spleen-associated TNF induction in LPS- treated mice, suggesting an effect on the synthesis of TNF. CPZ inhibited TNF induction by Gram-positive bacteria (heat-killed Staphylococcus epidermidis) and by anti-CD3 monoclonal antibodies. Intraperitoneal administration of CPZ also inhibited the induction of brain-associated TNF induced by intra-cerebroventricular injection of LPS. Therefore, CPZ is a more specific inhibitor of TNF production than DEX; in particular, CPZ increased the induction of 1L-l0, which is a `protective' cytokine known to inhibit LPS toxicity and TNF production. CPZ inhibited TNF production in vivo, irrespective of the TNF stimulus used to induce TNF. Finally, CPZ did not induce the `rebound' effect of DEX that, when given 24 hr before LPS, potentiates TNF production, but it did inhibit TNF production after 24 hr. [ABSTRACT FROM AUTHOR]
- Published
- 1994
3. Toxic Shock Syndrome Toxin 1 Resistance in Mice is Related to Interleukin 1 Production by Toxic Shock Syndrome Toxin 1.
- Author
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Ikejima, T., Orencole, S. F., Warner, S. J. C., and Dinarello, C. A.
- Published
- 1989
- Full Text
- View/download PDF
4. Array-based ELISAs for high-throughput analysis of human cytokines.
- Author
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Moody MD, Van Arsdell SW, Murphy KP, Orencole SF, and Burns C
- Subjects
- Anti-Inflammatory Agents pharmacology, Antibodies, Monoclonal, Cell Line, Cytokines immunology, Humans, Interferon-alpha analysis, Interferon-alpha immunology, Interferon-gamma analysis, Interferon-gamma immunology, Interleukin-1 analysis, Interleukin-1 immunology, Interleukin-10 analysis, Interleukin-10 immunology, Interleukin-6 analysis, Interleukin-6 immunology, Monocytes cytology, Monocytes drug effects, Monocytes immunology, Sensitivity and Specificity, Tumor Necrosis Factor-alpha analysis, Tumor Necrosis Factor-alpha immunology, Cytokines analysis, Enzyme-Linked Immunosorbent Assay methods
- Abstract
In this report, we describe the development of a mini-array system suitable for high-throughput quantification of proteins. This mini-array is a multiplexed, sandwich-type ELISA that measures the concentration of seven different human cytokines--TNF-alpha, IFN alpha, IFN gamma, IL-1 alpha, IL-1 beta, IL-6, and IL-10--from a single sample in each well of a 96-well plate. The mini-array is produced by spotting monoclonal antibodies (mAbs) in a 3 x 3 pattern in the bottom of the wells of 96-well polystyrene plates. Cytokines that are captured by the arrayed mAbs are detected by using biotinylated mAbs, followed by the addition of a streptavidin-horseradish peroxidase (HRP) conjugate and a chemiluminescent substrate. The light produced from the HRP-catalyzed oxidation of the substrate is measured at each spot in the array by imaging the entire plate with a commercially available CCD camera. Here, we demonstrate that these 96-well-plate format mini-arrays have performance characteristics that make them suitable for the high-throughput screening of anti-inflammatory compounds.
- Published
- 2001
- Full Text
- View/download PDF
5. An antibody to a 17 amino acid synthetic peptide of the type I interleukin-1 receptor preferentially blocks interleukin-1 beta binding.
- Author
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Clark BD, Ikejima T, Mancilla J, Orencole SF, Sirko SP, Ishii N, Okuda K, and Dinarello CA
- Subjects
- Amino Acid Sequence, Animals, Binding Sites, Cell Line, Humans, Immunoglobulins chemistry, Mice, Molecular Sequence Data, Molecular Weight, Precipitin Tests, Receptors, Interleukin-1 chemistry, Receptors, Interleukin-1 metabolism, Solubility, Tumor Cells, Cultured, Water chemistry, Antigen-Antibody Reactions, Interleukin-1 metabolism, Peptide Fragments immunology, Protein Structure, Secondary, Receptors, Interleukin-1 immunology
- Abstract
On the basis of their relative hydropathy and alpha-helical structure, we prepared antibodies to four synthetic peptides with amino acid sequences homolgous to four hydrophilic, extracellular regions of the murine 80 kDa type I interleukin-1 receptor (IL-1RI). Antibodies to each of the four peptides recognized their specific immunogen. Human [125I]-IL-1 alpha or -beta was crosslinked to murine EL4 and D10S cells. Antiserum to peptide 150-166 precipitated the IL-1/IL-1R complex, whereas antibodies to peptide 66-84, 190-200, or 266-285 did not. Antibody to peptide 150-166 did not precipitate the type II IL-1R. Anti-IL-1RI150-166 blocked 71% of the binding of radiolabeled human IL-1 beta to EL4 cells and 50% of the binding to D10S cells. Using affinity-purified anti-IL-1RI150-166, we compared the ability of this antibody to inhibit the binding of murine or human IL-1 alpha to that of murine or human IL-1 beta. At a concentration of 20 ng/ml, affinity-purified anti-IL-1RI150-166 blocked 50% binding of murine IL-1 beta. At 1 microgram/ml, 90% blockage was observed. In contrast, no significant blockade of IL-1 alpha binding was observed at concentrations as high as 3 micrograms/ml of anti-IL-1RI150-166. The selective blockade of IL-1 beta forms was not due to differences in the affinities of these ligands for receptors on these cells. The antibody also blocked the binding of human IL-1 beta but not human IL-1 alpha to EL4 cells. The biologic activity of murine IL-1 beta but not IL-1 alpha on EL4 cells was also inhibited by this antibody. These data suggest (1) that antibody to a specific epitope on the extracellular domain interferes with the binding of IL-1 beta but not IL-1 alpha, (2) the differential inhibition of binding of IL-1 beta but not IL-1 alpha by anti-IL-1RI150-166 also blocks biologic activity, and (3) IL-1 alpha and IL-1 beta may transduce different signals by binding to separate loci on the IL-1RI.
- Published
- 1996
- Full Text
- View/download PDF
6. Hematologic and immunomodulatory effects of an interleukin-1 receptor antagonist coinfusion during low-dose endotoxemia in healthy humans.
- Author
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Granowitz EV, Porat R, Mier JW, Orencole SF, Callahan MV, Cannon JG, Lynch EA, Ye K, Poutsiaka DD, and Vannier E
- Subjects
- Adolescent, Adult, Cell Count, Cell Division drug effects, Concanavalin A pharmacology, Cytokines blood, Endotoxins antagonists & inhibitors, Endotoxins blood, Escherichia coli, Humans, Interleukin 1 Receptor Antagonist Protein, Lymphocyte Activation drug effects, Male, Neutrophils drug effects, Recombinant Proteins pharmacology, Sialoglycoproteins administration & dosage, Sialoglycoproteins pharmacokinetics, Endotoxins pharmacology, Interleukin-1 antagonists & inhibitors, Sialoglycoproteins pharmacology
- Abstract
Endotoxin is a component of gram-negative bacteria that causes hematologic and immunologic changes through its induction of cytokines. Interleukin-1 receptor antagonist (IL-1Ra) is a naturally occurring inhibitor of IL-1 that competes with IL-1 for occupancy of cell-surface receptors but possesses no agonist activity. We investigated the ability of human recombinant IL-1Ra to block the effects of low-dose endotoxin. Fourteen healthy male volunteers between 18 and 30 years old were injected intravenously with 3 ng/kg Escherichia coli endotoxin. Concurrent with the injections, nine volunteers received a 3-hour continuous intravenous infusion of IL-1Ra. The other five subjects were given a 3-hour infusion of saline. Volunteers injected with endotoxin experienced a threefold increase in circulating neutrophils over baseline. This neutrophilia was significantly reduced by 48% in subjects administered endotoxin plus IL-1Ra (P = .0253). Ex vivo mitogen-induced peripheral blood mononuclear cell proliferation decreased by greater than 60% at 3 and 6 hours after endotoxin injection (P = .0053). This endotoxin-induced reduction in mitogen response was reversed in subjects coinjected with IL-1Ra (P = .0253). Endotoxin-induced symptoms, fever, and tachycardia were unaffected by IL-1Ra. IL-1 appears to be an important mediator in endotoxemia because some of its hematologic and immunomodulatory effects can be blocked by IL-1Ra.
- Published
- 1993
7. Differential regulation of cytokine production in lipopolysaccharide tolerance in mice.
- Author
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Erroi A, Fantuzzi G, Mengozzi M, Sironi M, Orencole SF, Clark BD, Dinarello CA, Isetta A, Gnocchi P, and Giovarelli M
- Subjects
- Animals, Drug Tolerance, Female, Interleukin-1 pharmacology, Interleukin-6 pharmacology, Mice, Mice, Inbred Strains, Tetradecanoylphorbol Acetate pharmacology, Tumor Necrosis Factor-alpha pharmacology, Cytokines biosynthesis, Lipopolysaccharides administration & dosage
- Abstract
We investigated the pattern of down-regulation of cytokine production in endotoxin (lipopolysaccharide [LPS]) tolerance. A 4-day treatment with LPS (35 micrograms per mouse) was followed by a challenge on day 6 with one more injection of LPS. Circulating tumor necrosis factor (TNF) and interleukin-6 (IL-6) could not be induced (> 99% inhibition) by LPS in LPS-tolerant mice; colony-stimulating factor (CSF) was also down-regulated by more than 95%, whereas interferon (IFN) and IL-1 syntheses were only partially inhibited. To study the mechanism of cytokine down-regulation in tolerance, we attempted to reverse the tolerant state by pretreatment with phorbol 12-myristate 13-acetate (PMA) (4 micrograms per mouse) 10 min before the LPS challenge. PMA completely restored IL-6 production and partially that of CSF. PMA had no effect on IFN production and inhibited the induction of IL-1. TNF production was also not restored by PMA. To investigate the role of endogenously produced cytokines in the development of LPS tolerance, we administered IL-6, TNF, or IL-1 alpha, using the same treatment schedule as that for LPS. Whereas IL-6 had no effect, IL-1 alpha or TNF induced partial tolerance to LPS in terms of inhibition of LPS-stimulated TNF and IL-6 production. However, a full LPS-tolerant state could not be induced by administration of recombinant cytokines, suggesting the existence of additional mechanisms, such as a loss of LPS receptors or changes in release of soluble binding proteins.
- Published
- 1993
- Full Text
- View/download PDF
8. Intravenous endotoxin suppresses the cytokine response of peripheral blood mononuclear cells of healthy humans.
- Author
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Granowitz EV, Porat R, Mier JW, Orencole SF, Kaplanski G, Lynch EA, Ye K, Vannier E, Wolff SM, and Dinarello CA
- Subjects
- Antigens, CD analysis, Antigens, Differentiation, Myelomonocytic analysis, Antigens, Differentiation, T-Lymphocyte analysis, CD3 Complex analysis, CD56 Antigen, Enterotoxins pharmacology, Escherichia coli immunology, Humans, Interleukin-1 pharmacology, Leukocytes, Mononuclear metabolism, Lipopolysaccharide Receptors, Male, Bacterial Toxins, Cytokines biosynthesis, Endotoxins pharmacology, Immune Tolerance, Leukocytes, Mononuclear immunology, Superantigens
- Abstract
When administered parenterally, endotoxin stimulates the synthesis of IL-1, TNF-alpha, and IL-6. However, this initial injection induces tolerance; a second injection of endotoxin results in lower levels of circulating cytokines. In our study, five healthy male volunteers between the ages of 18 and 30 were injected with Escherichia coli endotoxin. Four subjects received only saline. Immediately before the injection and 3, 6, and 24 h afterward, PBMC were isolated and stimulated in vitro with endotoxin, IL-1, or toxic shock syndrome toxin-1. Inasmuch as CD14+ monocytes are the primary source of the cytokines induced by these stimuli, results are expressed as cytokine production per 10(6) CD14+ cells. Six h after endotoxin injection, endotoxin-stimulated CD14+ cells synthesized 66% less IL-1 beta (p < 0.01), 47% less TNF-alpha (p < 0.001), 56% less IL-6 (p < 0.01), and 49% less IL-8 (p < 0.01) than cells obtained before the injection. This suppression was not specific for endotoxin; IL-1 beta-induced IL-1 alpha and TNF-alpha were reduced by 84% (p = 0.01) and 68% (p < 0.001), respectively. A decrease in cytokine synthesis was also observed using toxic shock syndrome toxin-1 as a stimulus: 57% for IL-1 beta (p = 0.06), 70% for TNF-alpha (p < 0.01), 56% for IL-6 (p < 0.05), and 71% for IL-8 (p = 0.001). When data were expressed as cytokine production per 10(6) PBMC, cells isolated 3 h after endotoxin injection synthesized significantly less stimulus-induced IL-1, TNF-alpha, IL-6, and IL-8 than did PBMC from saline-injected controls. We conclude that endotoxin tolerance is due, in part, to changes in the stimulus-induced cytokine response of circulating CD14+ cells.
- Published
- 1993
9. Detection of tumor necrosis factor soluble receptor p55 in blood samples from healthy and endotoxemic humans.
- Author
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Shapiro L, Clark BD, Orencole SF, Poutsiaka DD, Granowitz EV, and Dinarello CA
- Subjects
- Adolescent, Adult, Binding, Competitive, Escherichia coli, Humans, Male, Polyethylene Glycols, Radioimmunoassay, Receptors, Tumor Necrosis Factor, Reproducibility of Results, Solubility, Toxemia etiology, Tumor Necrosis Factor-alpha metabolism, Endotoxins blood, Receptors, Cell Surface analysis, Toxemia blood
- Abstract
The tumor necrosis factor (TNF) soluble receptor derived from the cell surface p55 TNF receptor (TNFsRp55) is a naturally occurring substance generated during infection and inflammation. TNFsRp55 inhibits biologic effects of TNF. An RIA was developed to quantitate TNFsRp55 in human blood. Recovery of TNFsRp55 from blood anticoagulated with EDTA was optimal compared with recovery from serum or heparinized plasma. TNF did not interfere with the assay. With the RIA based on radiolabeled nonglycosylated Escherichia coli-derived recombinant TNFsRp55, a mean concentration of 198 +/- 15 pg/mL was found in 14 volunteers. When glycosylated CHO cell-derived TNFsRp55 was used, the mean level was 1656 +/- 95 pg/mL. Infusion of endotoxin into volunteers induced TNFsRp55, which peaked at 517 +/- 99 pg/mL for the E. coli-based RIA and 7300 +/- 1810 pg/mL for the CHO cell-based RIA. These findings demonstrate that blood collected in EDTA is optimal for measuring circulating TNFsRp55 and that this soluble receptor is present in health but elevated during endotoxemia.
- Published
- 1993
- Full Text
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10. Granulocyte-macrophage colony-stimulating factor synthesis during experimental endotoxemia in humans.
- Author
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Granowitz EV, Porat R, Orencole SF, Callahan MV, Lynch EA, Wolff SM, and Dinarello CA
- Subjects
- Adult, Granulocyte-Macrophage Colony-Stimulating Factor blood, Humans, Radioimmunoassay, Recombinant Proteins analysis, Endotoxins toxicity, Escherichia coli, Granulocyte-Macrophage Colony-Stimulating Factor biosynthesis, Shock, Septic blood
- Published
- 1992
- Full Text
- View/download PDF
11. N-acetylcysteine and glutathione as inhibitors of tumor necrosis factor production.
- Author
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Peristeris P, Clark BD, Gatti S, Faggioni R, Mantovani A, Mengozzi M, Orencole SF, Sironi M, and Ghezzi P
- Subjects
- Animals, Cells, Cultured, Corticosterone blood, Dactinomycin pharmacology, Interleukin-1 biosynthesis, Interleukin-6 biosynthesis, Lipopolysaccharides toxicity, Macrophages drug effects, Macrophages immunology, Male, Methionine Sulfoximine analogs & derivatives, Methionine Sulfoximine pharmacology, Mice, Mice, Inbred Strains, Serum Amyloid A Protein analysis, Serum Amyloid A Protein drug effects, Acetylcysteine pharmacology, Glutathione metabolism, Tumor Necrosis Factor-alpha biosynthesis
- Abstract
TNF is a major mediator in the pathogenesis of endotoxic shock, and its inhibition has a protective effect in various animal models of sepsis or endotoxin (lipopolysaccharide, LPS) toxicity. LPS treatment also induces an oxidative damage mediated by increased production of reactive oxygen intermediates. N-Acetylcysteine (NAC) is an antioxidant and a precursor of the synthesis of glutathione (GSH) and was reported to protect against LPS toxicity and LPS-induced pulmonary edema. In this study we investigated the effect of NAC on TNF production and LPS lethality in mice. The results indicated that oral administration of NAC protects against LPS toxicity and inhibits the increase in serum TNF levels in LPS-treated mice. The inhibition was not confined to the released form of TNF, since NAC also inhibited LPS-induced spleen-associated TNF. On the other hand, the inhibitor of GSH synthesis, DL-buthionine-(SR)-sulfoximine (BSO), had the opposite effect of potentiating LPS-induced TNF production, and this was associated with a decrease in liver GSH levels. Repletion of liver GSH with NAC reversed this effect. NAC was also active in inhibiting TNF production and hepatotoxicity in mice treated with LPS in association with a sensitizing dose of Actinomycin D. These data indicate that GSH can be an endogenous modulator of TNF production in vivo. On the other hand, NAC pretreatment did not inhibit other effects of LPS, particularly induction of serum IL-6, spleen IL-1 alpha, and corticosterone, in the same experimental model, suggesting that the observed effect could be specific for TNF.
- Published
- 1992
- Full Text
- View/download PDF
12. Acute phase response in exercise. II. Associations between vitamin E, cytokines, and muscle proteolysis.
- Author
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Cannon JG, Meydani SN, Fielding RA, Fiatarone MA, Meydani M, Farhangmehr M, Orencole SF, Blumberg JB, and Evans WJ
- Subjects
- Acute-Phase Reaction blood, Adult, Aged, Aging physiology, Blood Cell Count, Dinoprostone metabolism, Double-Blind Method, Humans, Interleukin-1 blood, Interleukin-6 blood, Leukocytes cytology, Leukocytes physiology, Male, Methylhistidines urine, Radioimmunoassay, Tumor Necrosis Factor-alpha metabolism, Acute-Phase Reaction physiopathology, Cytokines physiology, Exercise physiology, Muscles metabolism, Proteins metabolism, Vitamin E physiology
- Abstract
Cytokines such as interleukin 1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), and interleukin 6 (IL-6) mediate a variety of host responses to trauma and infection, including skeletal muscle proteolysis. This investigation assesses the influence of damaging eccentric exercise on in vitro production and plasma concentrations of cytokines and their relationship to muscle protein breakdown. In a double-blind placebo-controlled protocol, 21 male subjects took vitamin E supplements (800 IU/day) for 48 days, then ran downhill on an inclined treadmill. Twenty-four hours after this single session of eccentric exercise, endotoxin-induced secretion of IL-1 beta was augmented 154% (P less than 0.01) in cells obtained from the placebo subjects, but no significant exercise-related changes were observed in cells from the vitamin E-supplemented subjects. TNF-alpha secretion was also significantly increased 24 h after exercise, but the response was not inhibited by vitamin E. In contrast, IL-6 secretion did not change after exercise, but dietary vitamin E supplementation significantly reduced IL-6 secretion throughout the 12-day period of observation (P = 0.023). Urinary 3-methylhistidine excretion correlated with mononuclear cell secretion of both IL-1 beta (P less than 0.05) and prostaglandin E2 (P less than 0.05), supporting the concept that these mononuclear cell products contribute to the regulation of muscle proteolysis.
- Published
- 1991
- Full Text
- View/download PDF
13. Acute phase response in exercise: interaction of age and vitamin E on neutrophils and muscle enzyme release.
- Author
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Cannon JG, Orencole SF, Fielding RA, Meydani M, Meydani SN, Fiatarone MA, Blumberg JB, and Evans WJ
- Subjects
- Acute-Phase Reaction blood, Acute-Phase Reaction metabolism, Adult, Creatine Kinase blood, Humans, Leukocyte Count, Lipid Peroxides blood, Male, Neutrophils pathology, Superoxides metabolism, Acute-Phase Reaction physiopathology, Aging physiology, Exercise, Muscles enzymology, Neutrophils metabolism, Vitamin E physiology
- Abstract
Several host defense responses and metabolic reactions that occur during infection have been observed after exercise. We hypothesized that these reactions, known as the "acute phase response," contribute to the breakdown and clearance of damaged tissue after exercise. This hypothesis was tested with 21 male volunteers representing two ranges of age (22-29 and 55-74 yr), who ran downhill on an inclined treadmill to accentuate damaging eccentric muscular contractions. The subject groups were further divided in a double-blind placebo-controlled protocol, which examined the influence of 48 days of dietary vitamin E supplementation before the exercise. All subjects were monitored for 12 days after exercise for changes in circulating leukocytes, superoxide release from neutrophils, lipid peroxidation, and efflux of the intramuscular enzyme creatine kinase (CK) into the circulation. Among those receiving placebo, the less than 30-yr-old subjects responded to exercise with a significantly greater neutrophilia and higher plasma CK concentrations than the greater than 55-yr-old subjects. Dietary supplementation with vitamin E tended to eliminate the differences between the two age groups, primarily by increasing the responses of the greater than 55-yr-old subjects. At the time of peak concentrations in the plasma, CK correlated significantly with superoxide release from neutrophils. The association of enzyme efflux with neutrophil mobilization and function supports the concept that neutrophils are involved in the delayed increase in muscle membrane permeability after damaging exercise.
- Published
- 1990
- Full Text
- View/download PDF
14. Interleukin-1 induced T-lymphocyte proliferation and its relation to IL-1 receptors.
- Author
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Dinarello CA, Orencole SF, and Savage N
- Subjects
- Animals, Cell Division, Cell Line, Receptors, Interleukin-1, Recombinant Proteins, T-Lymphocytes metabolism, Interleukin-1 physiology, Receptors, Immunologic metabolism, T-Lymphocytes cytology
- Published
- 1989
- Full Text
- View/download PDF
15. Studies on IL-1 receptors on D10S T-helper cells: demonstration of two molecularly and antigenically distinct IL-1 binding proteins.
- Author
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Savage N, Puren AJ, Orencole SF, Ikejima T, Clark BD, and Dinarello CA
- Subjects
- Animals, Binding Sites, Binding, Competitive, Cell Count, Cell Division, Cell Line, Interleukin-1 pharmacology, Interleukin-2 pharmacology, Interleukin-4 pharmacology, Mice, Receptors, Interleukin-1, Recombinant Proteins pharmacology, Interleukin-1 metabolism, Receptors, Immunologic metabolism, T-Lymphocytes, Helper-Inducer metabolism
- Abstract
Receptor binding studies were performed on the interleukin-1 (IL-1) sensitive T-helper cell line D10S, a stable line which proliferates to subfemtomolar concentrations of IL-1 in the absence of mitogens. IL-1 binds in a specific and saturable manner and Scatchard analysis at 4 degrees C reveals one class of binding affinity. On D10S cells, the Kd for IL-1 is 227 pM +/- 80, with 11,000 (range 3,300 to 23,800) sites per cell. EL4.6.1 cells, which are less sensitive to IL-1, bind with a single class of high affinity sites (55 pM; 4,000 sites). D10S cells incubated 18 h with IL-1 display reduced IL-1 receptor (IL-1R) numbers and affinities, consistent with reduced (75%, p less than 0.005) proliferation to subsequent IL-1; preincubation with IL-4 increases the number of IL-1R which is associated with increased (200%, p less than 0.001) proliferation to IL-1. The molecular mass of the major (80 kD) IL-1R binding [125I]IL-1 alpha on D10S cells was consistently observed at 73 kD as compared to the 80 kD molecule on the EL4 cells. On the other hand, crosslinking studies with [125I]IL-1 beta on D10S cells revealed a novel 46 kD band on gradient SDS-PAGE corresponding to a binding protein of 29 to 30 kD, which is antigenically distinct from the 80 kD IL-1R. Crosslinking of D10S or EL4 cells at 4 degrees C in the presence of phytohemagglutinin (PHA) and labeled IL-1 enhanced the appearance of the 30 kD IL-1 binding protein. The findings are consistent with a two-chain model for the IL-1R, although Scatchard analysis did not consistently indicate two classes of affinities. IL-1 binding to the 80 kD protein may form a heteroduplex with the 30 kD IL-1R which could account for the presence of the 120 to 130 kD IL-1 crosslinked proteins observed by several investigators.
- Published
- 1989
- Full Text
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16. Interleukin 1 induces interleukin 1. I. Induction of circulating interleukin 1 in rabbits in vivo and in human mononuclear cells in vitro.
- Author
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Dinarello CA, Ikejima T, Warner SJ, Orencole SF, Lonnemann G, Cannon JG, and Libby P
- Subjects
- Animals, Gene Expression Regulation, Humans, RNA, Messenger genetics, Rabbits, Radioimmunoassay, Recombinant Proteins pharmacology, Time Factors, Fever physiopathology, Interleukin-1 physiology, Leukocytes physiology
- Abstract
Interleukin 1 (IL-1) plays an important role in host defense mechanisms by increasing body temperature, inducing the synthesis of a variety of lymphokines and hepatic acute phase proteins and acting as a chemoattractant for lymphocytes. However, in some microenvironments such as injured tissue or joint spaces, elevated IL-1 levels may contribute to pathologic processes, for example, proliferation and fibrosis of tissue involved in pannus formation as well as degradation of matrix and abnormal tissue architecture. To investigate potential mechanisms that may lead to excessive production of IL-1, we have examined the ability of IL-1 to participate in an amplification event by inducing its own gene expression leading to synthesis of biologically active IL-1. When injected into rabbits, recombinant human IL-1-alpha induced biphasic fevers, and during the second temperature elevation 3 hr later, a circulating pyrogenic material was detected by passive transfer of plasma to other rabbits. Induction of the biphasic fever was not caused by endotoxin contamination of the recombinant IL-1. The 3-hr circulating pyrogen was heat-labile and was not residual injected IL-1-alpha. Chromatographic separation of this plasma and biologic assay suggested that it was new IL-1 of rabbit origin. We next incubated human blood mononuclear cells with recombinant IL-1-alpha and measured the intracellular and extracellular levels of IL-1 by bioassay using the D10.G4.1 murine T cell line. In order to control for the carryover of recombinant IL-1-alpha used to stimulate the mononuclear cells (MNC), we used neutralizing antibodies that were specific for IL-1-alpha or IL-1-beta. The results of these neutralizations showed that recombinant human IL-1-alpha induces the synthesis of IL-1-beta in human MNC in vitro. These results were verified with a radioimmunoassay specific for IL-1-beta. At concentrations of 100 ng/ml, IL-1-alpha induced prostaglandin E2 production in the MNC culture, and this was associated with decreased production of immunoreactive IL-1-beta. Adding indomethacin to the cultures prevented the decreased production of IL-1-beta induced by high concentrations of IL-1-alpha. Using nonadherent MNC, we observed an increase in IL-1-beta as well as IL-1-alpha mRNA after 4 hr of exposure to recombinant IL-1-alpha. These results demonstrate that IL-1-alpha induces biologically active and immunoreactive IL-1-beta from MNC in vitro and that the same concentrations of IL-1-alpha induce gene expression for both forms of IL-1.(ABSTRACT TRUNCATED AT 400 WORDS)
- Published
- 1987
17. Characterization of a subclone (D10S) of the D10.G4.1 helper T-cell line which proliferates to attomolar concentrations of interleukin-1 in the absence of mitogens.
- Author
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Orencole SF and Dinarello CA
- Subjects
- Animals, Cell Cycle, Cell Division drug effects, Cell Line, Dose-Response Relationship, Drug, Lymphocyte Subsets drug effects, Mice, Phytohemagglutinins pharmacology, Rats, Recombinant Proteins pharmacology, Interleukin-1 pharmacology, T-Lymphocytes, Helper-Inducer drug effects
- Abstract
Most studies have shown that interleukin-1 (IL-1) acts as a helper or co-stimulator in T-lymphocyte activation and proliferation by mitogens or antigens. We describe here a stable subclone (D10S) of the murine D10.G4.1 helper T-cell which proliferates to subfemtomolar (attomolar) concentrations of IL-1 beta or alpha in the absence of mitogens. D10S cells have been maintained in culture for over two years without splenic cell feeder layers nor antigen stimulation. Detection of proliferation can be made by either uptake of tritiated thymidine at 72 h or in 48 h by a colorimetric assay which measures mitochondrial dehydrogenases; the latter assay is rapid and inexpensive. D10S cells are distinct from the parent clone D10.G4., which requires mitogens for IL-1 activity. IL-1-induced proliferation is independent of the elaboration of IL-2, IL-4, or IL-6, although these cells proliferate to these lymphokines at considerably higher concentrations when compared to IL-1. The D10S cells proliferate in direct correlation to the duration of IL-1 presence in the culture. We found no evidence that IL-1 induced more IL-1 in these cells. The subclone is highly specific for IL-1: proliferation was not observed to endotoxin, human or murine interferon-gamma (IFN gamma), tumor necrosis factor (TNF), lymphotoxin, or granulocyte-macrophage colony stimulating factor (GM-CSF). There was no suppressive effect of transforming growth factor (TGF beta). Only at high concentrations (100 ng/ml) did IL-6 induce proliferation. We conclude that this stable, feeder layer-free cell line is highly sensitive to IL-1 which acts as a direct stimulant for these cells; they are also useful for bioassays as well as the study of IL-1 receptors as described in the accompanying paper.
- Published
- 1989
- Full Text
- View/download PDF
18. Increased interleukin 1 beta in human skeletal muscle after exercise.
- Author
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Cannon JG, Fielding RA, Fiatarone MA, Orencole SF, Dinarello CA, and Evans WJ
- Subjects
- Adult, Biopsy, Humans, Immunoenzyme Techniques, Male, Muscles analysis, Muscles cytology, Recombinant Proteins analysis, Interleukin-1 analysis, Muscles physiology, Physical Exertion
- Abstract
Interleukin 1 beta (IL-1 beta) is a protein released from blood monocytes and related cells in response to infectious or inflammatory stimuli. Although IL-1 beta is elevated in the circulation for only a few hours after an acute inflammatory challenge or exercise, it has been proposed to mediate anabolic and catabolic processes that can last for several days. In this report, eccentric exercise was used as a noninfectious inflammatory stimulus. IL-1 beta was found in muscle tissue up to 5 days after exercise using specific immunohistochemical tissue staining. Increased IL-1 beta immunoreactivity was observed in muscle tissue from four human subjects who performed the exercise, but not in tissue obtained at the same time intervals from two subjects who did not exercise. Little immunohistochemical evidence of interleukin-1 alpha or tumor necrosis factor alpha was observed before or after exercise. These results implicate IL-1 beta in the metabolic adaptations of muscle tissue, which occur in response to noninfectious stresses.
- Published
- 1989
- Full Text
- View/download PDF
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