17 results on '"Pagliari, Gabriella"'
Search Results
2. Bronchoalveolar lavage fluid and progression of scleroderma interstitial lung disease
- Author
-
De Santis, Maria, Bosello, Silvia Laura, Peluso, Giusy, Pinnelli, Michela, Alivernini, Stefano, Zizzo, Gaetano, Bocci, Mario, Capacci, Annunziata, La Torre, Giuseppe, Mannocci, Alice, Pagliari, Gabriella, Varone, Francesco, Pistelli, Roberto, Danza, Francesco Maria, and Ferraccioli, Gianfranco
- Published
- 2012
- Full Text
- View/download PDF
3. β-thymosins and interstitial lung disease: study of a scleroderma cohort with a one-year follow-up
- Author
-
Messana Irene, Cabras Tiziana, Bocci Mario, Zizzo Gaetano, Iavarone Federica, Fanali Chiara, Peluso Giusy, Bosello Silvia L, Inzitari Rosanna, De Santis Maria, Fuso Leo, Varone Francesco, Pagliari Gabriella, Castagnola Massimo, and Ferraccioli Gianfranco
- Subjects
Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background β-thymosins play roles in cytoskeleton rearrangement, angiogenesis, fibrosis and reparative process, thus suggesting a possible involvement in the pathogenesis of systemic sclerosis. The aim of the study was to investigate the presence of thymosins β4, β4 sulfoxide, and β10 in bronchoalveolar lavage fluid of scleroderma patients with interstitial lung disease and the relation of these factors with pulmonary functional and radiological parameters. Methods β-thymosins concentrations were determined by Reverse Phase-High Performance Liquid Chromatography-Electrospray-Mass Spectrometry in the bronchoalveolar lavage fluid of 46 scleroderma patients with lung involvement and of 15 controls. Results Thymosin β4, β4 sulfoxide, and β10 were detectable in bronchoalveolar lavage fluid of patients and controls. Thymosin β4 levels were significantly higher in scleroderma patients than in controls. In addition, analyzing the progression of scleroderma lung disease at one-year follow-up, we have found that higher thymosin β4 levels seem to have a protective role against lung tissue damage. Thymosin β4 sulfoxide levels were higher in the smokers and in the scleroderma patients with alveolitis. Conclusions We describe for the first time β-thymosins in bronchoalveolar lavage fluid and their possible involvement in the pathogenesis of scleroderma lung disease. Thymosin β4 seems to have a protective role against lung tissue damage, while its oxidation product mirrors an alveolar inflammatory status.
- Published
- 2011
- Full Text
- View/download PDF
4. The Role of Bronchoalveolar Lavage in the Microbiological Diagnosis of Pneumonia in Patients with Haematological Malignancies
- Author
-
Pagano, Livio, Pagliari, Gabriella, Basso, Agostino, Marra, Roberto, Sica, Simona, Frigieri, Luigi, Morace, Giulia, Ardito, Fausta, and Leone, Giuseppe
- Published
- 1997
5. Functional, radiological and biological markers of alveolitis and infections of the lower respiratory tract in patients with systemic sclerosis
- Author
-
Danza Francesco, Pistelli Riccardo, Pagliari Gabriella, Tolusso Barbara, Capuano Anna, La Torre Giuseppe, Bosello Silvia, De Santis Maria, Zoli Angelo, and Ferraccioli Gianfranco
- Subjects
Diseases of the respiratory system ,RC705-779 - Abstract
Abstract Background A progressive lung disease and a worse survival have been observed in patients with systemic sclerosis and alveolitis. The objective of this study was to define the functional, radiological and biological markers of alveolitis in SSc patients. Methods 100 SSc patients (76 with limited and 24 with diffuse disease) underwent a multistep assessment of cardiopulmonary system: pulmonary function tests (PFTs) every 6–12 months, echocardiography, high resolution computed tomography (HRCT) and bronchoalveolar lavage (BAL), if clinically advisable. Alveolar and interstitial scores on HRCT and IL-6 plasma levels were also assessed as lung disease activity indices. Results 90 SSc patients with abnormal PFTs and 3 with signs and/or symptoms of lung involvement and normal PFTs underwent HRCT and echocardiography. HRCT revealed evidence of fibrosis in 87 (93.5%) patients, with 55 (59.1%) showing both ground glass attenuation and fibrosis. In 42 patients who had exhibited ground glass on HRCT and consented to undergo BAL, 16 (38.1%) revealed alveolitis. 12 (75%) of these patients had restrictive lung disease (p < 0.0001) and presented diffuse skin involvement (p = 0.0009). IL-6 plasma levels were higher in patients with alveolitis than in patients without (p = 0.041). On logistic regression model the best independent predictors of alveolitis were diffuse skin involvement (OR(95%CIs):12.80(2.54–64.37)) and skin score > 14 (OR(95%CIs):7.03(1.40–34.33)). The alveolar score showed a significant correlation with IL-6 plasma levels (r = 0.36, p = 0.001) and with the skin score (r = 0.33, p = 0.001). Cultures of BAL fluid resulted positive in 10 (23.8%) of the 42 patients that underwent BAL and after one year a deterioration in PFTs occurred in 8 (80%) of these patients (p = 0.01). Pulmonary artery systolic pressure ≥ 40 mmHg was found in 6 (37.5%) patients with alveolitis. Conclusion We found alveolitis only in 38.1% of the patients who had exhibited ground glass on HRCT and then underwent BAL, probably because the concomitant fibrosis influenced results. A diffuse skin involvement and a restrictive pattern on PFTs together with ground glass on HRCT were judged possible markers of alveolitis, a BAL examination being indicated as the next step. Nevertheless BAL would be necessary to detect any infections of the lower respiratory tract that may cause further deterioration in lung function.
- Published
- 2005
- Full Text
- View/download PDF
6. Within-day and between-day repeatability of measurements with an electronic nose in patients with COPD.
- Author
-
Bofan, Maria, Mores, Nadia, Baron, Marco, Dabrowska, Malgorzata, Valente, Salvatore, Schmid, Maurizio, Trové, Andrea, Conforto, Silvia, Zini, Gina, Cattani, Paola, Fuso, Leonello, Mautone, Antonella, Mondino, Chiara, Pagliari, Gabriella, D'Alessio, Tommaso, and Montuschi, Paolo
- Published
- 2013
- Full Text
- View/download PDF
7. β-thymosins and interstitial lung disease: study of a scleroderma cohort with a one-year follow-up.
- Author
-
De Santis, Maria, Inzitari, Rosanna, Bosello, Silvia L., Peluso, Giusy, Fanali, Chiara, Iavarone, Federica, Zizzo, Gaetano, Bocci, Mario, Cabras, Tiziana, Messana, Irene, Fuso, Leo, Varone, Francesco, Pagliari, Gabriella, Castagnola, Massimo, and Ferraccioli, Gianfranco
- Subjects
THYMOSIN ,SCLERODERMA (Disease) ,CYTOSKELETON ,NEOVASCULARIZATION ,SYSTEMIC scleroderma ,BRONCHOALVEOLAR lavage - Abstract
Background: β-thymosins play roles in cytoskeleton rearrangement, angiogenesis, fibrosis and reparative process, thus suggesting a possible involvement in the pathogenesis of systemic sclerosis. The aim of the study was to investigate the presence of thymosins β
4 , β4 sulfoxide, and β10 in bronchoalveolar lavage fluid of scleroderma patients with interstitial lung disease and the relation of these factors with pulmonary functional and radiological parameters. Methods: β-thymosins concentrations were determined by Reverse Phase-High Performance Liquid Chromatography-Electrospray-Mass Spectrometry in the bronchoalveolar lavage fluid of 46 scleroderma patients with lung involvement and of 15 controls. Results: Thymosin β4 , β4 sulfoxide, and β10 were detectable in bronchoalveolar lavage fluid of patients and controls. Thymosin β4 levels were significantly higher in scleroderma patients than in controls. In addition, analyzing the progression of scleroderma lung disease at one-year follow-up, we have found that higher thymosin β4 levels seem to have a protective role against lung tissue damage. Thymosin β4 sulfoxide levels were higher in the smokers and in the scleroderma patients with alveolitis. Conclusions: We describe for the first time β-thymosins in bronchoalveolar lavage fluid and their possible involvement in the pathogenesis of scleroderma lung disease. Thymosin β4 seems to have a protective role against lung tissue damage, while its oxidation product mirrors an alveolar inflammatory status. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
8. β-thymosins and interstitial lung disease: a study of a sclerodema cohort with a one-year follow-up.
- Author
-
De Santis, Maria, Inzitari, Rosanna, Bosello, Silvia L., Peluso, Giusy, Fanali, Chiara, Iavarone, Federica, Zizzo, Gaetano, Bocci, Mario, Cabras, Tiziana, Messana, Irene, Leo Fuso, Varone, Francesco, Pagliari, Gabriella, Castagnola, Massimo, and Ferraccioli, Gianfranco
- Subjects
CYTOSKELETON formation ,NEOVASCULARIZATION ,FIBROSIS ,LUNG diseases ,IMMUNOTHERAPY - Abstract
Background: b-thymosins play roles in cytoskeleton rearrangement, angiogenesis, fibrosis and reparative process, thus suggesting a possible involvement in the pathogenesis of systemic sclerosis. The aim of the study was to investigate the presence of thymosins β
4 , β4 sulfoxide, and β10 in bronchoalveolar lavage fluid of scleroderma patients with interstitial lung disease and the relation of these factors with pulmonary functional and radiological parameters. Methods: β-thymosins concentrations were determined by Reverse Phase-High Performance Liquid Chromatography-Electrospray-Mass Spectrometry in the bronchoalveolar lavage fluid of 46 scleroderma patients with lung involvement and of 15 controls. Results: Thymosin β4 , β4 sulfoxide, and β10 were detectable in bronchoalveolar lavage fluid of patients and controls. Thymosin β4 levels were significantly higher in scleroderma patients than in controls. In addition, analyzing the progression of scleroderma lung disease at one-year follow-up, we have found that higher thymosin β4 levels seem to have a protective role against lung tissue damage. Thymosin β4 sulfoxide levels were higher in the smokers and in the scleroderma patients with alveolitis. Conclusions: We describe for the first time β-thymosins in bronchoalveolar lavage fluid and their possible involvement in the pathogenesis of scleroderma lung disease. Thymosin β4 seems to have a protective role against lung tissue damage, while its oxidation product mirrors an alveolar inflammatory status. [ABSTRACT FROM AUTHOR]- Published
- 2011
- Full Text
- View/download PDF
9. Pharmacotherapy of asthma: regular treatment or on demand?
- Author
-
Montuschi, Paolo, Pagliari, Gabriella, and Fuso, Leonello
- Abstract
Some studies have raised the question of the need for chronic controller therapy in mild persistent asthma as suggested by international guidelines. Although the Improving Asthma Control (IMPACT) and Beclomethasone plus Salbutamol (BEST) studies suggest that on-demand therapy in some patients with mild persistent asthma achieves a similar degree of asthma control based on symptoms and functional outcomes, the IMPACT study indicates that regular and on-demand therapy is not equivalent for controlling airway inflammation. Persistent airway inflammation might lead to airway remodelling with onset or worsening of symptoms, deterioration in lung function, and reduced response to pharmacological therapy. However, the relationships between chronic airway inflammation and airway remodelling need to be clarified. Choosing the ‘right’ pharmacological strategy (regular versus on-demand treatment) for asthma control is currently difficult due to the fact that (1) inflammatory outcome measures were not generally incorporated into asthma clinical trials; (2) the relationships between chronic airway inflammation and airway remodelling are largely unknown; (3) current clinical asthma trials that are generally based on symptomatic and functional outcome measures are too short to assess the impact of regular anti-inflammatory therapy on natural history of asthma; (4) asthma is an heterogeneous disease and different phenotypes of asthma patients likely requiring a different therapeutic approach can be identified, even in the same class of asthma severity. Guidelines for asthma management are valuable tools, although they are necessarily based on a strategy directed to the best outcome in a group of patients. Asthma phenotyping is becoming central for asthma management. The issue of regular versus on-demand treatment of intermittent and mild persistent asthma would be better addressed if considered within an individualized approach to asthma management and assessment. Identification of clinical, functional, morphological and biochemical phenotypes of patients with asthma and its clinical implications is likely to lead to a tailored, individualized, pharmacological therapy and asthma management. [ABSTRACT FROM PUBLISHER]
- Published
- 2009
- Full Text
- View/download PDF
10. Functional, radiological and biological markers of alveolitis and infections of the lower respiratory tract in patients with systemic sclerosis.
- Author
-
De Santis, Maria, Bosello, Silvia, La Torre, Giuseppe, Capuano, Anna, Tolusso, Barbara, Pagliari, Gabriella, Pistelli, Riccardo, Danza, Francesco Maria, Zoli, Angelo, and Ferraccioli, Gianfranco
- Subjects
ALVEOLITIS ,RESPIRATORY infections ,SYSTEMIC scleroderma ,LUNG diseases ,PATIENTS ,CARDIOPULMONARY system ,PULMONARY function tests ,MEDICAL research - Abstract
Background: A progressive lung disease and a worse survival have been observed in patients with systemic sclerosis and alveolitis. The objective of this study was to define the functional, radiological and biological markers of alveolitis in SSc patients. Methods: 100 SSc patients (76 with limited and 24 with diffuse disease) underwent a multistep assessment of cardiopulmonary system: pulmonary function tests (PFTs) every 6-12 months, echocardiography, high resolution computed tomography (HRCT) and bronchoalveolar lavage (BAL), if clinically advisable. Alveolar and interstitial scores on HRCT and IL-6 plasma levels were also assessed as lung disease activity indices. Results: 90 SSc patients with abnormal PFTs and 3 with signs and/or symptoms of lung involvement and normal PFTs underwent HRCT and echocardiography. HRCT revealed evidence of fibrosis in 87 (93.5%) patients, with 55 (59.1%) showing both ground glass attenuation and fibrosis. In 42 patients who had exhibited ground glass on HRCT and consented to undergo BAL, 16 (38.1%) revealed alveolitis. 12 (75%) of these patients had restrictive lung disease (p < 0.0001) and presented diffuse skin involvement (p = 0.0009). IL-6 plasma levels were higher in patients with alveolitis than in patients without (p = 0.041). On logistic regression model the best independent predictors of alveolitis were diffuse skin involvement (OR(95%CIs):12.80(2.54-64.37)) and skin score > 14 (OR(95%CIs):7.03(1.40-34.33)). The alveolar score showed a significant correlation with IL-6 plasma levels (r = 0.36, p = 0.001) and with the skin score (r = 0.33, p = 0.001). Cultures of BAL fluid resulted positive in 10 (23.8%) of the 42 patients that underwent BAL and after one year a deterioration in PFTs occurred in 8 (80%) of these patients (p = 0.01). Pulmonary artery systolic pressure = 40 mmHg was found in 6 (37.5%) patients with alveolitis. Conclusion: We found alveolitis only in 38.1% of the patients who had exhibited ground glass on HRCT and then underwent BAL, probably because the concomitant fibrosis influenced results. A diffuse skin involvement and a restrictive pattern on PFTs together with ground glass on HRCT were judged possible markers of alveolitis, a BAL examination being indicated as the next step. Nevertheless BAL would be necessary to detect any infections of the lower respiratory tract that may cause further deterioration in lung function. [ABSTRACT FROM AUTHOR]
- Published
- 2005
- Full Text
- View/download PDF
11. Effect of aqueous cigarette smoke extract on the chemiluminescence kinetics of polymorphonuclear leukocytes and on their glycolytic and phagocytic activity.
- Author
-
Zappacosta, Bruno, Persichilli, Silvia, Minucci, Angelo, Stasio, Enrico Di, Carlino, Paola, Pagliari, Gabriella, Giardina, Bruno, and Sole, Pasquale De
- Published
- 2001
- Full Text
- View/download PDF
12. Bilateral Bronchoalveolar Lavage in Progressive Systemic Sclerosis: Interlobar Variability, Lymphocyte Subpopulations, and Functional Correlations.
- Author
-
Frigieri, Luigi, Mormile, Flaminio, Grilli, Nunziatina, Mancini, Donatella, Ciappi, Giuliano, Pagliari, Gabriella, Magarò, Mario, and Flamini, Giovanna
- Published
- 1991
- Full Text
- View/download PDF
13. The yield of bronchoalveolar lavage in the etiological diagnosis of pneumonia in leukemia and lymphoma patients.
- Author
-
Marra, Roberto, Pagano, Livio, Pagliari, Gabriella, Frigieri, Luigi, Storti, Sergio, Morace, Guilia, Ardito, Fausta, and Leone, Giuseppe
- Published
- 1993
- Full Text
- View/download PDF
14. Role of ultrasound-guided transbronchial biopsy in the diagnosis of peripheral pulmonary lesions.
- Author
-
Fuso, Leonello, Varone, Francesco, Magnini, Daniele, Baldi, Fabiana, Rindi, Guido, Pagliari, Gabriella, Inchingolo, Riccardo, and Smargiassi, Andrea
- Subjects
- *
ULTRASONIC imaging , *BIOPSY , *PRECANCEROUS conditions , *FLUOROSCOPY , *BRONCHOSCOPY , *CANCER diagnosis - Abstract
Abstract: Introduction: Endobronchial ultrasound (EBUS) can be used as an alternative to fluoroscopy to visualize a peripheral pulmonary lesion (PPL) and to provide an image guidance for transbronchial biopsy (TBB). The aim of this study was to verify the accuracy of EBUS-guided TBB in the diagnosis of PPLs. Methods: All the patients with CT-scan evidence of PPL who underwent bronchoscopy with EBUS in the period between 2008 and 2011 were retrospectively evaluated. EBUS was performed using a radial-type miniature ultrasound probe. Once obtained an EBUS image of the PPL, we measured the distance of the PPL from the outer orifice of the working channel of the bronchoscope in order to perform TBB at PPL site. Results: A total of 662 patients were examined. The mean diameter of lesions was 36±20mm. PPLs were visualized in 494 patients (75%) and the TBB was performed in 479 patients. Thirty-two patients were lost in follow-up and data from 447 patients were analyzed. TBB results were 255 cancers and 192 non-malignant lesions. The final diagnosis reported was 359 cases of cancer and 88 of benign lesion. EBUS-guided TBB had a sensitivity of 71% for the diagnosis of cancer, a negative predictive value of 46% and an overall diagnostic accuracy of 77%. Conclusions: These data obtained from a large series of patients and using an original method show that EBUS represents a valid support to bronchoscopy and that the EBUS-guided TBB has a high diagnostic yield in the diagnosis of PPLs. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
15. Exhaled and non-exhaled non-invasive markers for assessment of respiratory inflammation in patients with stable COPD and healthy smokers.
- Author
-
Santini G, Mores N, Shohreh R, Valente S, Dabrowska M, Trové A, Zini G, Cattani P, Fuso L, Mautone A, Mondino C, Pagliari G, Sala A, Folco G, Aiello M, Pisi R, Chetta A, Losi M, Clini E, Ciabattoni G, and Montuschi P
- Subjects
- Aged, Biomarkers analysis, Cross-Sectional Studies, Dinoprost analogs & derivatives, Dinoprostone analysis, Exhalation, Female, Humans, Inflammation metabolism, Isoprostanes analysis, Male, Middle Aged, Nitric Oxide analysis, Pulmonary Disease, Chronic Obstructive metabolism, Severity of Illness Index, Breath Tests methods, Inflammation diagnosis, Oxidative Stress physiology, Pulmonary Disease, Chronic Obstructive diagnosis, Smoking metabolism, Sputum chemistry
- Abstract
We aimed at comparing exhaled and non-exhaled non-invasive markers of respiratory inflammation in patients with chronic obstructive pulmonary disease (COPD) and healthy subjects and define their relationships with smoking habit. Forty-eight patients with stable COPD who were ex-smokers, 17 patients with stable COPD who were current smokers, 12 healthy current smokers and 12 healthy ex-smokers were included in a cross-sectional, observational study. Inflammatory outcomes, including prostaglandin (PG) E2 and 15-F2t-isoprostane (15-F2t-IsoP) concentrations in exhaled breath condensate (EBC) and sputum supernatants, fraction of exhaled nitric oxide (FENO) and sputum cell counts, and functional (spirometry) outcomes were measured. Sputum PGE2 was elevated in both groups of smokers compared with ex-smoker counterpart (COPD: P < 0.02; healthy subjects: P < 0.03), whereas EBC PGE2 was elevated in current (P = 0.0065) and ex-smokers with COPD (P = 0.0029) versus healthy ex-smokers. EBC 15-F2t-IsoP, a marker of oxidative stress, was increased in current and ex-smokers with COPD (P < 0.0001 for both) compared with healthy ex-smokers, whereas urinary 15-F2t-IsoP was elevated in both smoker groups (COPD: P < 0.01; healthy subjects: P < 0.02) versus healthy ex-smokers. FENO was elevated in ex-smokers with COPD versus smoker groups (P = 0.0001 for both). These data suggest that the biological meaning of these inflammatory markers depends on type of marker and biological matrix in which is measured. An approach combining different types of outcomes can be used for assessing respiratory inflammation in patients with COPD. Large studies are required to establish the clinical utility of this strategy.
- Published
- 2016
- Full Text
- View/download PDF
16. Comparison of real-time PCR, conventional PCR, and galactomannan antigen detection by enzyme-linked immunosorbent assay using bronchoalveolar lavage fluid samples from hematology patients for diagnosis of invasive pulmonary aspergillosis.
- Author
-
Sanguinetti M, Posteraro B, Pagano L, Pagliari G, Fianchi L, Mele L, La Sorda M, Franco A, and Fadda G
- Subjects
- Adult, Aged, Antigens, Fungal analysis, Aspergillosis, Allergic Bronchopulmonary mortality, Aspergillus genetics, Bronchoalveolar Lavage Fluid chemistry, Enzyme-Linked Immunosorbent Assay methods, Female, Galactose analogs & derivatives, Humans, Male, Middle Aged, Survival Analysis, Aspergillosis, Allergic Bronchopulmonary diagnosis, Aspergillus isolation & purification, Mannans analysis, Polymerase Chain Reaction methods
- Abstract
An iCycler iQ real-time PCR assay targeting 18S rRNA Aspergillus-specific sequences was developed for the diagnosis of invasive pulmonary aspergillosis (IPA). Positive findings were obtained for 18 of 20 (90%) bronchoalveolar lavage (BAL) fluid specimens from patients with probable or confirmed IPA and were obtained for none of the 24 BAL samples from patients with no clinical evidence of aspergillosis. These results were concordant with those of a nested PCR assay, which detected 90% of the patients with IPA, while galactomannan ELISA revealed positivity for 100% of these patients, suggesting that combined use of methods might improve the diagnosis of IPA.
- Published
- 2003
- Full Text
- View/download PDF
17. A case of nonspecific interstitial pneumonia associated with thymoma.
- Author
-
Maiolo C, Fuso L, Benedetto RT, Boniello V, Basso S, Granieri AM, Pagliari G, and Sallustio G
- Subjects
- Adult, Diagnosis, Differential, Humans, Lung Diseases, Interstitial diagnosis, Male, Respiratory Function Tests, Thymoma diagnosis, Thymus Neoplasms diagnosis, Tomography, X-Ray Computed, Lung Diseases, Interstitial complications, Thymoma complications, Thymus Neoplasms complications
- Published
- 2003
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.