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Abstract This study aimed to investigate antigenotoxicity and antioxidant potential of extract, fractions and vitexin from C. antisyphiliticus. Methanolic extract was fractionated through solvents of increasing polarity. The composition of extracts and fractions were evaluated through phytochemical screening. Micronucleus test was performed in mice to evaluate the antigenotoxicity. Antioxidant activity was measured using the assay 1,1-diphenyl-2-picrylhydrazyl (DPPH), iron ion chelating, thiobarbituric acid assay and nitric oxide scavenging. Treatment with extract, fractions and vitexin did not produce an increase in Micronucleus mean values. However, Micronucleus (MN) mean values decreased in relation to control. methanolic extract presented antioxidant potential for DPPH (81%), iron ion chelating (77.8%), Thiobarbituric Acid (TBARS) (32.49%) and Nitric Oxide (NO) (80.97%). Ethyl acetate fraction showed the highest antioxidant activity (65.46%). The vitexin showed a Inhibitory Concentration (IC50) of DPPH value smaller in relation to control. Vitexin flavonoid was detected by High Performance Liquid Chromatography (HPLC), infrared spectrometry and nuclear magnetic resonance. It can be inferred that methanolic extract, fraction ethyl acetate and vitexin isolated from C. antisyphiliticus is endowed with antigenotoxic and antioxidant potential.

The increasing tomato demand for the food market motivates improvements and the use of new biotechnologies in this fruit's production. The hybrid crop stands out for fruit production resistant to rot and postharvest wilt (long-life crops). Within this context, consumption of genetically modified food deserves attention regarding the safety and nutritional aspects due to the fact that inclusion and/or overexpression of genetic traits can cause harm to human health in the short or long term. In this scenario, this study aimed to evaluate genotoxicity and mutagenicity from different varieties of long-life tomatoes obtained by genetic breeding and also determines main bioactive compounds and antioxidant activity. The genotoxicity and mutagenicity were analyzed via the micronucleus test and the evaluation of chromosome aberrations in mice bone marrow respectively. We have also analyzed carotene, beta-carotene, lycopene, total phenol and flavonoid contents via spectrophotometry and antioxidant activity via DPPH radical scavenging assay. Considering the results obtained, it is possible to conclude that despite the absence of significant genotoxic activity among the evaluated samples, the antioxidant activity and the differences found in composition seems to be ruled by genetic factors, possibly due to the genetic breeding.

The increasing tomato demand for the food market motivates improvements and the use of new biotechnologies in this fruit's production. The hybrid crop stands out for fruit production resistant to rot and postharvest wilt (long-life crops). Within this context, consumption of genetically modified food deserves attention regarding the safety and nutritional aspects due to the fact that inclusion and/or overexpression of genetic traits can cause harm to human health in the short or long term. In this scenario, this study aimed to evaluate genotoxicity and mutagenicity from different varieties of long-life tomatoes obtained by genetic breeding and also determines main bioactive compounds and antioxidant activity. The genotoxicity and mutagenicity were analyzed via the micronucleus test and the evaluation of chromosome aberrations in mice bone marrow respectively. We have also analyzed carotene, beta-carotene, lycopene, total phenol and flavonoid contents via spectrophotometry and antioxidant activity via DPPH radical scavenging assay. Considering the results obtained, it is possible to conclude that despite the absence of significant genotoxic activity among the evaluated samples, the antioxidant activity and the differences found in composition seems to be ruled by genetic factors, possibly due to the genetic breeding.