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6. Relationships between Dental Topography, Gross Wear, and Bang and Ramm/Liversidge and Molleson Age Estimates for a Sample of Human Premolar Teeth.

Author

Roberts, Grace, Paul, Kathleen S., Hara, Anderson T., Algarni, Amnah, and Ungar, Peter S.

Subjects
BICUSPIDS, TOOTH abrasion, DENTAL maturity, TEETH, TOPOGRAPHY
Abstract

Copyright of Acta Stomatologica Croatica is the property of Acta Stomatologica Croatica and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2023
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7. A case study from Guyana of adapting engaged research design to promote ‘fairness in knowing’.

Author

Holliman, Richard, Marino, Alessandra, Grand, Ann, Berardi, Andrea, Mistry, Jay, Jafferally, Deirdre, Thomas, Raquel, Roberts, Grace, Marcus, Carol-Ann, Roopsind, Indranee, and Roberts, Anthony

Subjects
CRITICAL theory, TRADITIONAL knowledge, ORGANIZATIONAL change, EXPERIMENTAL design, COVID-19 pandemic
Abstract

In this paper, we have combined ideas drawn from philosophy (epistemic injustice), critical theory (epistemicide) and practical approaches (engaged research design) with Indigenous knowledge to promote ‘fairness in knowing’ in a project called DETECT (Integrated Space Technology Vector Control for Enhancing community health and resilience against escalating climatic disruptions), an early warning system to support communities in identifying mosquito breeding sites using satellite, drone and ground-sensing technologies. DETECT used engaged research design to inform preaward planning. We document how the project team, comprising Indigenous and other researchers, re-imagined the plans in the light of the COVID-19 pandemic to allow project participants to meet safely and equitably, and reflect on some of the key challenges in engaging across borders and cultures in the context of rapidly changing conditions characterised by vulnerability, risk, complexity and uncertainty. [ABSTRACT FROM AUTHOR]

Published
2022
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9. Students' participation in collaborative research should be recognised

Author

Borakati, Aditya, McLean, Kenneth, Drake, Thomas M., Harrison, Ewen M., Kamarajah, Sivesh K., Khatri, Chetan, Nepogodiev, Dmitri, Abbas, Minaam, Abdalkoddus, Muhammad, Abdel-Fattah, Areej, Abdelgalil, Reem, Abdikadir, Haweya, Adams, Ryan, Adams, Sarah, Adelaja, Inioluwa, Adeogun, Abiola, Adjei, Helena, Adlan, Amirul, Adwan, Hussamuddin, Aeyad, Sara, Aftab, Raiyyan, Afzul, Amir, Agarwal, Vani, Aglan, Hosam, Agrawal, Medha, Agrawal, Rishi, Ahmed, Fiza, Akhtar, Sobia, Akpenyi, Onyinye, Al-Attar, Maithem, Al-Ausi, Muhammed, Al-Khyatt, Waleed, Al-Mousawi, Alia, Al-Nasser, Zainab, Alagappan, Anand, Alberts, Justin, Alfa-Wali, Maryam, Ali, Abdulmajid, Ali, Adnan, Ali, Tamara, Alkhaffaf, Bilal, Allen, Rachael, Alubaidi, Kassem, Andah, Edemanwan, Anderson, Richard, Andrew, Kirstine, Ang, Andrew, Ang, Eshen, Anyomih, Theophilus, Archer, James, Archer, Matt, Arnell, Steven, Arnold, Matthew, Arora, Esha, Ashraf, Nadeem, Ashraf, Raees, Ashwood, Jordan, Asif, Usama, Atayi, Andrew, Auckburally, Sameera, Austin, Ralph, Azam, Sultana, Yahaya, Aishah Azri, Babatunde, Fiyin, Bach, Simon, Bachar, Roudi, Badran, Abdul, Baillie, Caroline, Balai, Edward, Baldwin, Alexander, Balian, Vartan, Banfield, Danielle, Bannard-Smith, Jonathan, Barker, Connor, Barmayehvar, Behrad, Barnfield, Jane, Bartlett, David, Bartlett, Richard, Baryeh, Kwaku, Basetti, Siddharth, Bateman, Kellie, Bath, Michael, Beamish, Andrew, Beasley, William, Beecroft, Simon, Begaj, Ardit, Beghal, Gurpreet, Belchos, Jessica, Bera, Katarzyna, Bergara, Tara, Betts, Anna, Bhangu, Aneel, Bhaskaran, Gayathri, Bhatti, Amina, Bica, Mihai, Billyard, Caitlin, Birkin, Emily, Blazeby, Jane, Blege, Harry, Blencowe, Natalie, Blore, Christopher, Boddy, Alex, Boissaud-Cooke, Matthew, Bolina, Anita, Bolton, William, Bosanquet, David, Bowley, Doug, Boyce, Kathryn, Branagan, Graham, Brayley, Jessica, Brecher, Joanna, Bresges, Kristina, Briggs, Emily, Broll, Ryan, Brown, Damien, Brown, Elliot, Brown, Leo, Brown, Robin, Brown, Rory, Bruce, Connor, Bruce, Pepa, Buckle, Rory, Budd, Emily, Buka, Richard, Burke, Dermot, Burke, Joshua, Burman, Alisha, Burney, Laura, Burrows, Amy, Bux, Mohammed, Cahill, Ronan, Calabria, Clementina, Camilleri-Brennan, Julian, Campbell, Amy, Campbell, Bill, Cant, Matthew, Cao, Yun, Carlson, Sophie, Carr, Grace, Carr, Luke, Carr, Rebecca, Carr, Richard, Cartwright, Eleanor, Castle, Alice, Cattle, Kirsty, Cave, Daniel, Chapman, Stephen, Charalabopoulos, Alexandros, Chaudhri, Sanjay, Chaudhry, Ahmad, Chauhan, Paresh, Chauhan, Priyesh, Chebbout, Ryad, Chen, Yunzi, Chenciner, Louisa, Cheng, Jingjie, Cheng, Natalie, Chew, Lin, China, Zenab, Chitnis, Abhishek, Chitsabesan, Praminthra, Choi, Paul, Choi, Sarah, Choudhry, Mariam, Choy, Chern, Ciurleo, Claudia, Claireaux, Henry, Coe, Peter, Cole, Simon, Concannon, Katy, Cope, Edward, Corbridge, Olivia, Court, Jessica, Cox, Louise, Craig-Mcquaide, Anna, Cresswell, Ben, Crozier, Lauren, Cruickshank, Neil, Cuckow, Lucy, Cui, Helen, Cumber, Elspeth, Cumming, Sarah, Cundy, Olivia, Cunha, Melissa, Cunha, Pedro, Cunliffe, Laura, Dada, Jazleen, Daliya, Prita, Dalli, Jeffrey, Daniels, Ian, Daniels, James, Daoub, Ahmed, Dar, Sabeera, Das, Emma, Das, Kaustuv, Davies, Emily, Davies, Gareth, Davies, Kirsty, Davies, Kristen, Davies, Rachel, Dawe, Victoria, Lucas de Carvalho, Joshua, De Jong, Katie, Deasy, Katherine, Deekonda, Praveena, Deepak, Sahil, Desai, Henal, Desai, Karishma, Devlin, Ryan, Dewan, Nishat, Dhillon, Akashdeep, Dhillon, Priya, Dhir, Tanya, Di Saverio, Salomone, Diamond, Julia, Dib, Peter, Dimitriadis, Panagiotis A., Dindyal, Shiva, Doe, Matthew, Doehrty, Ciaran, Dogra, Tara, Doshi, Arpan, Downey, Alison, Doyle, Joseph, Draper, Ashleigh, Duff, Sarah, Duncumb, Joseph, Dupre, Sophie, Durno, Justine, Dzieweczynski, Michal, Eardley, Nicola, Easby, Sarah, Easdon, Sam, Ebdewi, Hamdi, Eccles, Lydon, Edwards, Jacob, Eedarapalli, Padma, Elbuzidi, Mohamed, Elder, Patrick, Elliott, Lucy, Elsaddig, Malaz, Embury-Young, Ysabelle, Emesih, Sophie, Engledow, Alec, English, William, Episkopos, Christos, Epstein, Jonathan, Esmail, Rahim, Fatayer, Taher, Favero, Nicolò, Fearnhead, Nicola, Feldman, Maxine, Fennelly, Evelyn, Fenwick, Stephen, Ferguson, Lucie, Fergusson, Stuart, Fessas, Petros, FitzGerald, Isabel, Fitzgerald, J. Edward, Fitzpatrick, Harry, Fletcher, Daniel, Forjoe, Tonia, Forte, Beniamino, Fowler, Alex, France, Benjamin, Francis, Abraham, Francis, Niroshan, Francis, Sunil, Freeman, Sam, Fretwell, Vicky, Fung, Teresa, Furness, Hugh, Gallagher, Michael, Gallagher, Stuart, Gao, Chuanyu, Garard, Lothaire, Gardner, Shona, Gaukroger, Andrew, George, Daniel, George, Simi, Ghaddar, Jamal, Ghaffar, Ali, Ghouse, Shamira, Gilbert, Amanda, Gill, Ashveen, Giovinazzo, Francesco, Girling, Carey, Giwa, Lolade, Glasbey, James, Glen, Paul, Goble, Mary, Godfrey, Jenna, Goel, Shreya, Goh, Wenn, Gohil, Kajal, Gokani, Shyam, Gold, David, Golding, David, Gonzalez-Ciscar, Andrea, Goodson, Ross, Gough, Melissa, Govil, Shubhangi, Gower, Thomas, Graham, Christopher, Gray, Sam, Green, Patrick, Greenhalgh, Samuel, Gregoriou, Kyriacos, Gribbell, Rhiannon, Gribbon, Mary Catherine, Grieco, Charlotte, Griffiths, Emma, Griffiths, Ewen, Griffiths, Nathan, Griffiths, Sara, Grossart, Cathleen, Guerero, Daniel, Guillotte, Christianne, Gupta, Rishi, Guy, Claire, Gwozdz, Adam, Haddow, James, Hafiz, Shazia, Halkias, Constantine, Hall, Elisabeth, Hamid, Hasseb, Hamilton, Emma, Harbhajan Singh, Gurvinder Singh, Hardman, John, Harries, Rhiannon, Harris, Rhydian, Harrogate, Suzanne, Harty, Megan, Harvey, Jessica, Hashemi, Rahima, Hassane, Ahmed, Hawkins, Helen, Hawthorne, Thomas, Hayes, John, Hazenberg, Phoebe, Heath, Harry, Hebbar, Madhusoodhana, Heer, R., Hegarty O'Dowd, Roisin, Henshall, David, Herrod, Philip, Hester, Elizabeth, Heywood, Emily, Heywood, Nick, Hill, Frances, Hill, James, Hill, Kirsty, Ho, May, Hollyman, Marianne, Holroyd, David, Home, Joseph, Hornby, Steve, Horne, Laura, Horseman, Charlotte, Hosamuddin, Huma, Hough, Amy, Hourston, George, Hudson-Peacock, Nathan, Hughes, Belinda, Hughes, Katie, Huppatz, Isabel, Hurst, Penelope, Hussain, Mahrukh, Hussain, Shoaib Fahad, Hussain, Syeda, Hutchings, Imogen, Ibrahim, Bilal, Imam, Lema, Ingham, Rory, Ingleton, Rose, Iqbal, Rizwan, Isherwood, Jenny, Islim, Abdurrahman, Ismail, Omar, Iyer, Shashank, Jackman, Toby, Jain, Prashant, Jamal, Nadeem, Jamal, Sabine, James, Ellen, Jayaratne, Nirmitha, Jeffreys, Nathan, Jhala, Hiral, Johnson, Courtney, Johnston, Zoe, Jones, Conor, Jones, Emma-Jane, Jones, Keaton, Jones, Victor, Joseph, Roshan, Joshi, Dilan, Joyce, Holly, Joyner, Claire, Kale, Aditya, Kanabar, Sagar, Kanapeckaite, Lina, Kankam, Hadyn, Kaptanis, Sarantos, Karam, Edward, Karponis, Dimitrios, Karunatilleke, Anne, Kasivisvanathan, Veeru, Kaur, Geeta, Kauser, Samina, Keelty, Nigel, Kelly, Denise, Kennett, Jessica, Kerr, Molly, Kerwan, Ahmed, Khajuria, Apoorva, Khalil, Mostafa, Khaliq, Mehnoor, Khan, Ayushah, Khan, Hamzah, Khan, Haroon, Khan, Maaz, Khan, Maria, Khan, Shahab, Khan, Kaywaan, Khaw, Rachel, Kheterpal, Ashni, Khonsari, Parisa, Kiandee, Miraen, Kim, Samuel, Kim, Suji, Kim, Sung-Hee, King, Harry, Kinsella, Anna, Kishore, Ajit, Klimach, Stefan, Kolias, Angelos G., Kolodziejczyk, Anna, Kong, Chia Yew, Kong, Tseun Han James, Kouli, Omar, Kukran, Sebi, Kukran, Sevi, Kumaran, Geev, Kutuzov, Vladislav, Laing, Chris, Laing, Georgina, Lal, Kulvinder, Lalor, Peter, Lambert, Joel, Lambotharan, Sai Geethan, Lancaster, Eve, Latter, Jasmine, Latter, Michelle, Lau, Kenny, Lazarou, Alexa, Leadon, Madeline, Lee, Gabriel, Lee, Jeyoung, Lee, Kathryn, Lee, Matthew, Lee, Samuel, Lee, Zong, Leung, Edward, Lewis, Thomas, Li, Hansen, Li, Mimi, Liew, Wan Jane, Liew, Yao Ren, Light, Alexander, Lilis, Lydia, Lim, Diana, Lim, Hui, Lim, Joseph, Lim, Zhi, Liu, Siyin, Lloyd, James, Logan, Andrew, Loganathan, Priya, Long, M., Longstaff, Lydia, Rojas, Luisa Lopez, Lovegrove, Richard, Lowe-Zinola, Jack, Morrell, Byron Lu, Luck, Joshua, Luhmann, Andreas, Lunawat, Surabhika, Lund, Jon, Luo, Cong, Luo, Lorna, Lyell, Iona, Lykoudis, Panagis, Macdonald, Jonathan, Mackenzie, Aliya, Magee, Conor, Mahankali-Rao, Pooja, Mahawar, Kamal, Mahfooz, Mehreen, Mahmood, Faisal, Makwana, Samir, Malik, Tom, Mallick, Sohaib, Manalayil, Jyothis, Mandishona, Tinaye, Mangam, Sudhakar, Manimaran, Maniragav, Manimaran, Natarajan, Manson, Chris, Mansoor, Sufyan, Mansour, Fatima, Marcos Rodrigo, Alejandro, Markham, Nicholas, Marks, Maria, Marriott, Paul, Marsden, Hannah, Martin, Laura, Martins, Tiago, Mason, John, Mason, Luke, Masood, Mariam, Math, Nikhil, Mathew, Ginimol, Matthews, Jacob, Mayes, Jonathan, Mc Gee, Ursula, Mcallister, Ross, Mcallister, Sandra, Mccain, Scott, Mccann, Conor, Mccann, Emmet, McCarthy, Cathal, Mccoll, Gillian, Mcconaghie, Greg, Mcdermott, Ace, McDermott, Frank, Mcdougall, Rachel, McDowell, Mark, McFarlane, Gordon, McGregor, Richard, McKechnie, Doug, McKenna, Jillian, McKinstry, Scott, Mclachlan, Georgia, Mclean, E., McLennan, Elizabeth, McNair, Angus, Mealy, Kenneth, Mecia, Lauren, Mehta, Alexander, Mellan, Aidan, Menon, Arathi, Menzies, Donald, Mesbah, Zhubene, Messenger, David, Miller, George, Mishra, Aseem, Mistry, Sona, Mohamed, Tahira, Mushaini, Nisha Mohamed, Mohan, Midhun, Mohd Azmilssss, Ameerah, Mohite, Ajay, Moorthy, Krishna, Moradzadeh, Jalal, Morgan, Richard, Morley, Gabriella, Mortimer, Alice, Mownah, Hannah, Moxey, Paul, Mudalige, Gagira, Muhammad, Umarah, Munday, Samuel, Murphy, Ben, Murphy, Ciaran, Murray, Caoimhe, Murray, Hannah, Murray, Michael, Murtaza, Mohammed Ibrar, Mushtaq, Jameel, Mustafa, Ameer, Mustafa, Shams, Myers, Laura, Myers, Sam, Naasan, Adeeb, Nadeem, Kiran, Naeem, Hanzla, Naik, Prashant, Nair, Arun, Nambiar, Keshav K., Naqi, Muhammad, Naqvi, Zehra, Neo, Yan Ning, Neophytou, Georgia Irene, Neville, Jonathan, Newman, Tom, Ng, Benjamin, Ng, Guat, Ng, Jing Qi, Ng, Vincent, Ng, Zhan Herr, Ni Bhoirne, Maire, Nicholas, James, Nicholson, Gary, Ninkovic-Hall, George, Nixon, Gemma, Norwood, Mike, Noton, Toby, Nourzaie, Romman, Novell, Richard, Nyanhongo, Donald, O'Brien, James, O'Kane, Rory, O'Neill, Stephen, O'Sullivan, Hugh, Oakley, Thomas, Ogbuokiri, Chinomso, Ogunleye, Oluwafunto, Oh, Su, Okorocha, Emezie, Olivier, James, Ologunde, Rele, Omara, Sharif, Ormrod, Alice, Osborne, Caroline, Osmanska, Joanna, Owasil, Raisah, Owczarek, Sebastian, Ozcan, Ezgi, Palaniappan, Sri, Palazzo, Francesco, Palkhi, Abbas, Pandey, Gargi, Park, James, Parker, Jennifer, Parry, Anna, Parsonage, James, Passby, Lauren, Patel, Bhavi, Patel, Bhavik, Patel, Chantal, Patel, Dinisha, Patel, Kirtan, Patel, Panna, Patel, Pratiksha, Patel, Trupesh, Pathmarajah, Mariasoosai, Patil, Amogh, Patil, Pradeep, Patrick, Yusuf, Pearce, Jessica, Pearce, Lyndsay, Peirce, Colin, Peiris, Bryony, Pendrill, Amy, Periketi, Sreelata, Perry, Michael, Petrov, George, Phillips, Charlotte, Pike, Grace, Pinho-Gomes, Ana Catarina, Polly, Parhana, Ponweera, Arachchige, Poolovadoo, Yanish, Poonawala, Raunak, Popova, Petya, Pournaras, Dimitri, Powell, Brooke, Prabakaran, Praveena, Prakash, Esha, Pratumsuwan, Tapani, Prem Kumar, Anusha, Puddy, Helen, Pullinger, Michael, Punjabi, Nikita, Putt, Oliver Charles, Qadir, Omar, Qamar, Mubasher, Quinn, Patrick, Qureshi, Arham, Rabie, Mohamed, Radford, Angus, Radhakrishnan, Anand, Radotra, Ansh, Rafiq, Nasir, Rahem, Aria, Rahman, Nahim, Rahman, Syed, Rajagopal, Ramesh, Rajan, Nick, Rajaraman, Nikitha, Rajendran, Sumetha, Ramachenderam, Liandra, Ramakrishnan, Divya, Ramjas, Denisha, Rammell, James, Rampal, Ritika, Ramsay, George, Randhawa, Ratan, Rea, Ellis, Rees, Stephanie, Rehman, Saad, Rehman, Salwah, Rehnnuma, Nabila, Rejayee, Melina, Rob, Zakaria, Roberts, Charlotte, Roberts, Grace, Roberts, Ben, Robinson, Harry, Robinson, Stephen, Rogers, Ailin, Rogers, Alex, Rook, William, Ross, Talisa, Roy, Chloe, Rushd, Azelea, Rutherford, Duncan, Saat, Michael, Sadanand, Kaushik, Sagar, Rebecca, Sagoo, Harkiran, Saha, Arin, Sahnan, Kapil, Sait, Mohammed Salik, Sait, Saif, Salekin, Damien, Salem, Mostafa, Salloum, Nadia, Sanders, Emma, Sandhu, Jasmesh, Sandhu, N., Sandison, Lorna, Sandland-Taylor, Laura, Sangal, Ron, Sanghera, Chandan, Saramunda, Josephine, Satterthwaite, Lauren, Schramm, Moritz, Scott, Rupert, Searle, Chloe, Seehra, Harkiran, Segura-Sampedro, Juan Jose, Sekhon Inderjit Singh, Harpreet Kaur, Seraj, Shaikh Sanjid, Seth, Ishani, Sethi, Rajiv, Shah, Apar, Shaid, Mario, Shaikh, Shafaque, Shamali, Awad, Sharkey, Elizabeth, Sharma, Abhi, Sharma, Neil, Sharma, Sachin, Shenoy, Aniruddh, Shergill, Maleasha, Shirazi, Shahram, Siddiqui, Imran, Sim, Raykal, Simmonds, Lucy, Simon, Andrew, Simpson, William, Singh, Bharpoor, Singh, J., Singh, Prashant, Sinha, Anant, Sinha, Sidhartha, Sinnerton, Robert, Sivakumar, Chaamanti, Skelly, Brendan, Slater, Richard, Small, Samuel, Smart, Neil, Smart, Yat Wing, Smith, Alexander, Smith, Charlotte, Smith, Jason, Smith, Rebecca, Smith, Scott, Sodde, Peter, Soh, Zhi Min, Sonsale, Aniket, Soualhi, Ahmed, Spearman, John, Spencer, Robert, Spiers, Harry, Stather, Philip, Stoddart, Michael, Storey, Bradley, Stringer, Howard, Stringfellow, Thomas, Stubbs, Ben, Sukir, Niv, Sukirthan, Nivian, Suleman, Yasir, Sureshkumar, Aparnah, Suri, Ashwin, Swartbol, Timen, Tahir, Hyder, Tan, E. Tian, Tan, Huai Ling, Tan, Laura, Tang, Alethea, Taribagil, Priyal, Tay, Yao Zong, Taylor, Beth, Taylor, Zara, Thatcher, Alexandra, Thavayogan, Rachel, Thomaa, Michael, Thomas, Daniah, Thomas, Jenny, Thomas, Paul, Pinkney, Thomas, Thompson, Chris, Ting, Mag, Toner, Ethan, Tong, Godwin, Torkington, Jared, Traish, Molly, Triniman, Miles, Trotter, John, Tsang, Kwong, Turaga, Sanchit, Turley, Hannah, Turner, James, Urbonas, Tomas, Urquhart, Alexandra, Vadgama, Nimai, Vaidya, Aashay, van Boxel, Gijs, Vara, Swati, Varcada, Massimo, Varley, Rebecca, Varma, Dee, Vella-Baldacchino, Martinique, Venturini, Sara, Verma, Naina, Verma, Saurabh, Vernet, Gabrielle, Vipond, Mark, von Roon, Alex, Wadood, Qasim, Waite, Kathryn, Walker, Lewis, Walker, Nathan, Wan, Jonathan C.M., Wang, Liyang, Wang, Xue, Ward, Alex, Ward, Thomas, Warnaar, Nienke, Warren, Lloyd, Warren, Oliver, Waters, Sam, Watson, Angus, Watson, Laura Jayne, Waugh, Dominic, Weinberg, Daniel, West, Malcolm, White, Carla, White, Tim, Whitehurst, Katharine, Whitham, Robert, Wijekoon, Tharindri, Wijeyaratne, Manuk, Wilkin, Richard, Wilkins, Alex, Williams, Adam, Williams, Gethin, Williams, Luke, Williams, Robert, Williamson, Andrew, Willson, Jacinthe, Wilson, Andrew, Wilson, Holly, Wilson, James, Wilson, Lizzie, Wilson, Megan, Wilson, Michael, Wilson, Rebekah, Wilson, Tim, Woin, Evelina, Wright, Esther, Wright, Jenny, Wroe, Nicholas, Wylie, Joanne, Xu, Yiwang, Yalamarthi, Satheesh, Yan, Angela, Yang, Narisu, Yardimci, Eda, Yasin, Ibrahim, Yasin, Ismael, Yasin, Noor, Yates, Joseph, Yau, Jih Dar, Yeoh, Tricia, Yip, Joshua, Yong, Cissy, Zaver, Vasudev, Zhelezniakova, Tatiana, and Zulkifli, Adreana

Published
2017
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10. Population Pharmacokinetic/Pharmacodynamic Analysis of Intravenous Zanamivir in Healthy Adults and Hospitalized Adult and Pediatric Subjects With Influenza.

Author

Zuo, Peiying, Collins, Jon, Okour, Malek, Barth, Aline, Shortino, Denise, Yates, Phillip, Roberts, Grace, Watson, Helen A., Peppercorn, Amanda, and Hossain, Mohammad

Subjects
INFLUENZA, ADULTS, VIRUS-induced enzymes, CELL membranes, NEURAMINIDASE, INHALERS
Abstract

Zanamivir is a potent and highly selective inhibitor of influenza neuraminidase in which the inhibition of this enzyme prevents the virus from infecting other cells and specifically prevents release of the new virion from the host cell membrane. It is available as an oral powder for inhalation and intravenous formulations. The current population pharmacokinetic model based on data from eight studies of subjects treated with the intravenous formulation (125 healthy adults and 533 hospitalized adult and pediatric subjects with suspected or confirmed influenza) suggested a decreased zanamivir clearance in pediatric and renal impairment adult subjects. It also indicates that b.i.d. dosing is necessary to keep the exposure in influenza infected subjects above the 90% inhibitory concentration values of recently circulating viruses over the dosing interval. In the exposure‐response analysis (phases II and III studies), no apparent relationship was found between zanamivir exposure and clinically relevant pharmacodynamic end points. [ABSTRACT FROM AUTHOR]

Published
2020
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12. An Evaluation of Matrix-Containing and Humanised Matrix-Free 3-Dimensional Cell Culture Systems for Studying Breast Cancer.

Author

Roberts, Grace C., Morris, Paul G., Moss, Marcus A., Maltby, Sarah L., Palmer, Chelsea A., Nash, Claire E., Smart, Emily, Holliday, Deborah L., and Speirs, Valerie

Subjects
*BREAST cancer, *CELL culture, *FIBROBLASTS, *CANCER cells, *STROMAL cells
Abstract

Background: 3D cell cultures are emerging as more physiologically meaningful alternatives to monolayer cultures for many biological applications. They are attractive because they more closely mimic in vivo morphology, especially when co-cultured with stromal fibroblasts. Methodology/Principal Findings: We compared the efficacy of 3 different 3D cell culture systems; collagen I, low attachment culture vessels and a modification of Fibrolife®, a specialised humanised cell culture medium devoid of animal-derived components, using breast cancer cell lines representative of the different molecular subtypes of breast cancer, cultured alone or with human mammary fibroblasts with a view to developing matrix-free humanised systems. 3D collagen I culture supported the growth of a range of breast cancer cell lines. By modifying the composition of Fibrolife® to epiFL, matrix-free cell culture was possible. During sequential transfer to epiFL breast cancer cells gradually detached from the flask, growing progressively as spheroids. Phenotype was stable and reversible with cells remaining actively proliferating and easily accessible throughout culture. They could also be revived from frozen stocks. To achieve co-culture with fibroblasts in epiFL required use of low attachment culture vessels instead of standard plastic as fibroblasts remained adherent in epiFL. Here, cancer cell spheroids were allowed to form before adding fibroblasts. Immunohistochemical examination showed fibroblasts scattered throughout the epithelial spheroid, not dissimilar to the relationship of tumour stroma in human breast cancer. Conclusions: Because of its ease of handling, matrix-free 3D cell culture may be a useful model to study the influence of fibroblasts on breast cancer epithelial cells with use of epiFL culture medium taking this a step further towards a fully humanised 3D model. This methodology could be applied to other types of cancer cell lines, making this a versatile technique for cancer researchers wishing to use in vitro systems that better reflect cancer in vivo. [ABSTRACT FROM AUTHOR]

Published
2016
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13. Radiance in the Community: Living and Working in Wisdom.

Author

McMillen, Diane P. and Roberts, Grace

Subjects
HUMAN services, WISDOM, CONFIDENCE, WELL-being, PSYCHOLOGICAL resilience, HEALTH
Abstract

Some believe that our nation is in a "crisis" (e.g., funding, confidence, hope). However, those of us in human services remain cognizant that crisis can be synonymous with opportunity for change. This article will introduce an innovative and exciting inside-out approach to helping, known as the 3 principles. This understanding instills hope and promotes the well-being that exists in all individuals. As practitioners, we are better equipped to address the complexity of needs in our society when we are solidly grounded in our wisdom and focus on the health and resiliency of the people we serve. As our article demonstrates, and we know from the research and our experience as helping professionals, when we practice from this foundation individuals reconnect with their innate health, they regain their power, and they reignite the radiance in their communities. [ABSTRACT FROM AUTHOR]

Published
2014

14. Comparison of SARS-CoV-2 Evolution in Paediatric Primary Airway Epithelial Cell Cultures Compared with Vero-Derived Cell Lines.

Author

Bamford, Connor G. G., Broadbent, Lindsay, Aranday-Cortes, Elihu, McCabe, Mary, McKenna, James, Courtney, David G., Touzelet, Olivier, Ali, Ahlam, Roberts, Grace, Lopez Campos, Guillermo, Simpson, David, McCaughey, Conall, Fairley, Derek, Mills, Ken, and Power, Ultan F.

Subjects
EPITHELIAL cell culture, SARS-CoV-2, CELL lines, PHENOTYPIC plasticity, PEDIATRICS
Abstract

SARS-CoV-2 can efficiently infect both children and adults, albeit with morbidity and mortality positively associated with increasing host age and presence of co-morbidities. SARS-CoV-2 continues to adapt to the human population, resulting in several variants of concern (VOC) with novel properties, such as Alpha and Delta. However, factors driving SARS-CoV-2 fitness and evolution in paediatric cohorts remain poorly explored. Here, we provide evidence that both viral and host factors co-operate to shape SARS-CoV-2 genotypic and phenotypic change in primary airway cell cultures derived from children. Through viral whole-genome sequencing, we explored changes in genetic diversity over time of two pre-VOC clinical isolates of SARS-CoV-2 during passage in paediatric well-differentiated primary nasal epithelial cell (WD-PNEC) cultures and in parallel, in unmodified Vero-derived cell lines. We identified a consistent, rich genetic diversity arising in vitro, variants of which could rapidly rise to near fixation within two passages. Within isolates, SARS-CoV-2 evolution was dependent on host cells, with paediatric WD-PNECs showing a reduced diversity compared to Vero (E6) cells. However, mutations were not shared between strains. Furthermore, comparison of both Vero-grown isolates on WD-PNECs disclosed marked growth attenuation mapping to the loss of the polybasic cleavage site (PBCS) in Spike, while the strain with mutations in Nsp12 (T293I), Spike (P812R) and a truncation of Orf7a remained viable in WD-PNECs. Altogether, our work demonstrates that pre-VOC SARS-CoV-2 efficiently infects paediatric respiratory epithelial cells, and its evolution is restrained compared to Vero (E6) cells, similar to the case of adult cells. We highlight the significant genetic plasticity of SARS-CoV-2 while uncovering an influential role for collaboration between viral and host cell factors in shaping viral evolution and ultimately fitness in human respiratory epithelium. [ABSTRACT FROM AUTHOR]

Published
2022
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18. Evaluation of a range of mammalian and mosquito cell lines for use in Chikungunya virus research.

Author

Roberts GC, Zothner C, Remenyi R, Merits A, Stonehouse NJ, and Harris M

Subjects
A549 Cells, Animals, Cells, Cultured, Chlorocebus aethiops, HeLa Cells, Hep G2 Cells, Humans, In Vitro Techniques, Luciferases metabolism, Vero Cells, Aedes virology, Chikungunya Fever virology, Chikungunya virus pathogenicity, Viral Plaque Assay methods, Virus Replication
Abstract

Chikungunya virus (CHIKV) is becoming an increasing global health issue which has spread across the globe and as far north as southern Europe. There is currently no vaccine or anti-viral treatment available. Although there has been a recent increase in CHIKV research, many of these in vitro studies have used a wide range of cell lines which are not physiologically relevant to CHIKV infection in vivo. In this study, we aimed to evaluate a panel of cell lines to identify a subset that would be both representative of the infectious cycle of CHIKV in vivo, and amenable to in vitro applications such as transfection, luciferase assays, immunofluorescence, western blotting and virus infection. Based on these parameters we selected four mammalian and two mosquito cell lines, and further characterised these as potential tools in CHIKV research.

Published
2017
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19. Manipulation of both virus- and cell-specific factors is required for robust transient replication of a hepatitis C virus genotype 3a sub-genomic replicon.

Author

Kelly L, Badhan A, Roberts GC, Mbisa JL, and Harris M

Abstract

Hepatitis C virus (HCV) genotype (GT) 3 is the second most prevalent of the seven HCV genotypes and exhibits the greatest resistance to the highly potent, direct-acting antivirals (DAAs) that are currently in use. Previously a stable cell line harbouring the S52 GT3 sub-genomic replicon (SGR) was established, but this SGR was unable to robustly replicate transiently. As transient SGRs are a critical tool in the development of DAAs, and in the study of viral resistance, we sought to establish a transient SGR system based on S52. Next-generation sequencing was used to identify putative culture-adaptive substitutions that had arisen during long-term selection of the S52 SGR. A subset of these substitutions was built back into the S52 SGR in the context of a CpG/UpA-low luciferase reporter, with a single point mutation in NS4A conferring the greatest replication capability upon S52. Modification of the innate immune-sensing pathways of Huh7.5 hepatoma cells by expression of the parainfluenza virus type 5 V protein and SEC14L2 resulted in a further enhancement of S52 replication. Furthermore, this transiently replicating SGR showed genotype-specific differences in sensitivity to two clinically relevant NS5A DAAs. In conclusion, we report that a single substitution in NS4A, coupled with host cell modifications, enabled robust levels of transient replication by the GT3 S52 SGR. This system will have beneficial uses in both basic research into the unique aspects of GT3 biology and drug discovery.

Published
2017
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20. SNAP-tagged Chikungunya Virus Replicons Improve Visualisation of Non-Structural Protein 3 by Fluorescence Microscopy.

Author

Remenyi R, Roberts GC, Zothner C, Merits A, and Harris M

Subjects
Carrier Proteins, Cell Line, Tumor, Chikungunya Fever virology, Cytoplasmic Granules, Humans, src Homology Domains, Chikungunya virus physiology, Microscopy, Fluorescence methods, Replicon, Viral Nonstructural Proteins metabolism
Abstract

Chikungunya virus (CHIKV), a mosquito-borne alphavirus, causes febrile disease, muscle and joint pain, which can become chronic in some individuals. The non-structural protein 3 (nsP3) plays essential roles during infection, but a complete understanding of its function is lacking. Here we used a microscopy-based approach to image CHIKV nsP3 inside human cells. The SNAP system consists of a self-labelling enzyme tag, which catalyses the covalent linking of exogenously supplemented synthetic ligands. Genetic insertion of this tag resulted in viable replicons and specific labelling while preserving the effect of nsP3 on stress granule responses and co-localisation with GTPase Activating Protein (SH3 domain) Binding Proteins (G3BPs). With sub-diffraction, three-dimensional, optical imaging, we visualised nsP3-positive structures with variable density and morphology, including high-density rod-like structures, large spherical granules, and small, low-density structures. Next, we confirmed the utility of the SNAP-tag for studying protein turnover by pulse-chase labelling. We also revealed an association of nsP3 with cellular lipid droplets and examined the spatial relationships between nsP3 and the non-structural protein 1 (nsP1). Together, our study provides a sensitive, specific, and versatile system for fundamental research into the individual functions of a viral non-structural protein during infection with a medically important arthropod-borne virus (arbovirus).

Published
2017
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21. Antiviral activity of GW678248, a novel benzophenone nonnucleoside reverse transcriptase inhibitor.

Author

Ferris RG, Hazen RJ, Roberts GB, St Clair MH, Chan JH, Romines KR, Freeman GA, Tidwell JH, Schaller LT, Cowan JR, Short SA, Weaver KL, Selleseth DW, Moniri KR, and Boone LR

Subjects
Anti-HIV Agents therapeutic use, Antiviral Agents therapeutic use, Cell Culture Techniques, Cell Line, Tumor, Cells, Cultured, Cytotoxicity Tests, Immunologic, Drug Evaluation, Preclinical, Drug Resistance, Viral, HIV-1 genetics, HeLa Cells, Humans, Inhibitory Concentration 50, Jurkat Cells, Leukocytes, Mononuclear cytology, Leukocytes, Mononuclear virology, Molecular Structure, Mutation, Orosomucoid metabolism, Reverse Transcriptase Inhibitors chemistry, Reverse Transcriptase Inhibitors therapeutic use, Serum Albumin metabolism, U937 Cells, Virus Replication drug effects, Anti-HIV Agents pharmacology, Antiviral Agents pharmacology, Benzophenones chemistry, HIV-1 drug effects, Reverse Transcriptase Inhibitors pharmacology
Abstract

The compound GW678248 is a novel benzophenone nonnucleoside reverse transcriptase inhibitor (NNRTI). Preclinical assessment of GW678248 indicates that this compound potently inhibits wild-type (WT) and mutant human immunodeficiency virus type 1 (HIV-1) reverse transcriptase in biochemical assays, with 50% inhibitory concentrations (IC(50)s) between 0.8 and 6.8 nM. In HeLa CD4 MAGI cell culture virus replication assays, GW678248 has an IC(50) of < or =21 nM against HIV-1 isogenic strains with single or double mutations known to be associated with NNRTI resistance, including L100I, K101E, K103N, V106A/I/M, V108I, E138K, Y181C, Y188C, Y188L, G190A/E, P225H, and P236L and various combinations. An IC(50) of 86 nM was obtained with a mutant virus having V106I, E138K, and P236L mutations that resulted from serial passage of WT virus in the presence of GW678248. The presence of 45 mg/ml human serum albumin plus 1 mg/ml alpha-1 acid glycoprotein increased the IC(50) approximately sevenfold. Cytotoxicity studies with GW678248 indicate that the 50% cytotoxicity concentration is greater than the level of compound solubility and provides a selectivity index of >2,500-fold for WT, Y181C, or K103N HIV-1. This compound exhibits excellent preclinical antiviral properties and, as a prodrug designated GW695634, is being developed as a new generation of NNRTI for the treatment of HIV-1 in combination with other antiretroviral agents.

Published
2005
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22. Design of non-nucleoside inhibitors of HIV-1 reverse transcriptase with improved drug resistance properties. 2.

Author

Freeman GA, Andrews Iii CW 3rd, Hopkins AL, Lowell GS, Schaller LT, Cowan JR, Gonzales SS, Koszalka GW, Hazen RJ, Boone LR, Ferris RG, Creech KL, Roberts GB, Short SA, Weaver K, Reynolds DJ, Milton J, Ren J, Stuart DI, Stammers DK, and Chan JH

Subjects
Alkynes, Anti-HIV Agents chemistry, Anti-HIV Agents pharmacology, Benzoxazines, Binding Sites, Cell Line, Crystallography, X-Ray, Cyclopropanes, Drug Design, HIV Reverse Transcriptase genetics, HIV Reverse Transcriptase metabolism, HIV-1 drug effects, HIV-1 enzymology, Humans, Models, Molecular, Molecular Structure, Mutation, Oxazines chemistry, Quinolones chemistry, Quinolones pharmacology, Reverse Transcriptase Inhibitors chemistry, Reverse Transcriptase Inhibitors pharmacology, Structure-Activity Relationship, Anti-HIV Agents chemical synthesis, Drug Resistance, Viral, HIV Reverse Transcriptase chemistry, Quinolones chemical synthesis, Reverse Transcriptase Inhibitors chemical synthesis
Abstract

HIV-1 nonnucleoside reverse transcriptase inhibitors (NNRTIs) are part of the combination therapy currently used to treat HIV infection. The features of a new NNRTI drug for HIV treatment must include selective potent activity against both wild-type virus as well as against mutant virus that have been selected by use of current antiretroviral treatment regimens. Based on analogy with known HIV-1 NNRTI inhibitors and modeling studies utilizing the X-ray crystal structure of inhibitors bound in the HIV-1 RT, a series of substituted 2-quinolones was synthesized and evaluated as HIV-1 inhibitors.

Published
2004
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23. Novel benzophenones as non-nucleoside reverse transcriptase inhibitors of HIV-1.

Author

Chan JH, Freeman GA, Tidwell JH, Romines KR, Schaller LT, Cowan JR, Gonzales SS, Lowell GS, Andrews CW 3rd, Reynolds DJ, St Clair M, Hazen RJ, Ferris RG, Creech KL, Roberts GB, Short SA, Weaver K, Koszalka GW, and Boone LR

Subjects
Anti-HIV Agents chemistry, Anti-HIV Agents pharmacology, Benzophenones chemistry, Benzophenones pharmacology, Cell Line, Crystallography, X-Ray, Drug Resistance, Viral, HIV Reverse Transcriptase chemistry, HIV Reverse Transcriptase metabolism, HIV-1 drug effects, HIV-1 enzymology, HIV-1 genetics, Humans, Inhibitory Concentration 50, Mutation, Protein Binding, Reverse Transcriptase Inhibitors chemistry, Reverse Transcriptase Inhibitors pharmacology, Structure-Activity Relationship, Anti-HIV Agents chemical synthesis, Benzophenones chemical synthesis, HIV Reverse Transcriptase antagonists & inhibitors, Reverse Transcriptase Inhibitors chemical synthesis
Abstract

GW4511, GW4751, and GW3011 showed IC50 values < or =2 nM against wild type HIV-1 and <10 nM against 16 mutants. They were particularly potent against NNRTI-resistant viruses containing Y181C-, K103N-, and K103N-based double mutations, which account for a significant proportion of the clinical failure of the three currently marketed NNRTIs. The antiviral data together with the favorable pharmacokinetic data of GW4511 suggested that these benzophenones possess attributes of a new NNRTI drug candidate.

Published
2004
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