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Your search keyword '"Roesch, Luiz"' showing total 256 results
Start Over Author "Roesch, Luiz" Publication Type Academic Journals
256 results on '"Roesch, Luiz"'

18. A Step Forward to Empower Global Microbiome Research Through Local Leadership

Author

Pylro, Victor S, Mui, Tsai S, Rodrigues, Jorge LM, Andreote, Fernando D, Roesch, Luiz FW, and Microbiome, the Working Group Supporting the INCT

Subjects
Microbiology, Biochemistry and Cell Biology, Biological Sciences, Brazil, Humans, Leadership, Microbiota, Research, Working Group Supporting the INCT Microbiome, Brazilian microbiome, INCT Microbiome, local initiatives, microbiome priorities, Medical Microbiology, Biochemistry and cell biology
Published
2016

25. Differences in bacterial composition between men’s and women’s restrooms and other common areas within a public building

Author

Dobbler, Priscila Caroline Thiago, Laureano, Álvaro Macedo, Sarzi, Deise Schroder, Cañón, Ehidy Rocio Peña, Metz, Geferson Fernando, de Freitas, Anderson Santos, Takagaki, Beatriz Midori, D´Oliveira, Cristiane Barbosa, Pylro, Victor Satler, Copetti, André Carlos, Victoria, Filipe, Redmile-Gordon, Marc, Morais, Daniel Kumazawa, and Roesch, Luiz Fernando Wurdig

Published
2018
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33. Association between Dysbiosis in the Gut Microbiota of Primary Osteoporosis Patients and Bone Loss.

Author

Waldbaum, Julien D. H., Xhumari, Jessica, Akinsuyi, Oluwamayowa S., Arjmandi, Bahram, Anton, Stephen, and Roesch, Luiz Fernando Wurdig

Subjects
DYSBIOSIS, GUT microbiome, OSTEOPOROSIS
Abstract

In recent decades, gut microbiome research has experienced significant growth, driven by technological advances that enable quantifying bacterial taxa with greater precision. Age, diet, and living environment have emerged as three key factors influencing gut microbes. Dysbiosis, resulting from alterations in these factors, may lead to changes in bacterial metabolites that regulate pro- and anti-inflammatory processes and consequently impact bone health. Restoration of a healthy microbiome signature could mitigate inflammation and potentially reduce bone loss associated with osteoporosis or experienced by astronauts during spaceflight. However, current research is hindered by contradictory findings, insufficient sample sizes, and inconsistency in experimental conditions and controls. Despite progress in sequencing technology, defining a healthy gut microbiome across global populations remains elusive. Challenges persist in identifying accurate gut bacterial metabolics, specific taxa, and their effects on host physiology. We suggest greater attention be directed towards this issue in Western countries as the cost of treating osteoporosis in the United States reaches billions of dollars annually, with expenses projected to continue rising. [ABSTRACT FROM AUTHOR]

Published
2023
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36. Brazilian Microbiome Project: Revealing the Unexplored Microbial Diversity—Challenges and Prospects

Author

Pylro, Victor Satler, Roesch, Luiz Fernando Wurdig, Ortega, José Miguel, do Amaral, Alexandre Morais, Tótola, Marcos Rogério, Hirsch, Penny Ruth, Rosado, Alexandre Soares, Góes-Neto, Aristóteles, da Costa da Silva, Artur Luiz, Rosa, Carlos Augusto, Morais, Daniel Kumazawa, Andreote, Fernando Dini, Duarte, Gabriela Frois, de Melo, Itamar Soares, Seldin, Lucy, Lambais, Márcio Rodrigues, Hungria, Mariangela, Peixoto, Raquel Silva, Tsai, Ricardo Henrique Kruger Siu Mui, and Azevedo, Vasco

Published
2014

37. RESCUE: a validated Nanopore pipeline to classify bacteria through long-read, 16S-ITS-23S rRNA sequencing.

Author

Petrone, Joseph R., Glusberger, Paula Rios, George, Christian D., Milletich, Patricia L., Ahrens, Angelica P., Roesch, Luiz Fernando Wurdig, and Triplett, Eric W.

Subjects
BACTERIAL operons, RIBOSOMAL RNA, BACTERIAL ecology, HYPERVARIABLE regions, OPERONS, BACTERIA classification
Abstract

Despite the advent of third-generation sequencing technologies, modern bacterial ecology studies still use Illumina to sequence small (~400 bp) hypervariable regions of the 16S rRNA SSU for phylogenetic classification. By sequencing a larger region of the rRNA gene operons, the limitations and biases of sequencing small portions can be removed, allowing for more accurate classification with deeper taxonomic resolution. With Nanopore sequencing now providing raw simplex reads with quality scores above Q20 using the kit 12 chemistry, the ease, cost, and portability of Nanopore play a leading role in performing differential bacterial abundance analysis. Sequencing the near-entire rrn operon of bacteria and archaea enables the use of the universally conserved operon holding evolutionary polymorphisms for taxonomic resolution. Here, a reproducible and validated pipeline was developed, RRN-operon Enabled Species-level Classification Using EMU (RESCUE), to facilitate the sequencing of bacterial rrn operons and to support import into phyloseq. Benchmarking RESCUE showed that fully processed reads are now parallel or exceed the quality of Sanger, with median quality scores of approximately Q20+, using the R10.4 and Guppy SUP basecalling. The pipeline was validated through two complex mock samples, the use of multiple sample types, with actual Illumina data, and across four databases. RESCUE sequencing is shown to drastically improve classification to the species level for most taxa and resolves erroneous taxa caused by using short reads such as Illumina. [ABSTRACT FROM AUTHOR]

Published
2023
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38. Effects of Microencapsulated Blend of Organic Acids and Pure Botanicals on the Ruminal Microbiota in an In Vitro Dual-Flow Continuous Culture System.

Author

Lobo, Richard R., Roesch, Luiz Fernando Wurdig, Grilli, Ester, and Faciola, Antonio P.

Subjects
CITRIC acid, ORGANIC acids, PRINCIPAL components analysis, MICROORGANISM populations, BACTERIAL diversity, DIETARY supplements, THYMOL
Abstract

The objective of the study was to evaluate the effects of dietary supplementation of a microencapsulated blend of organic acids and pure botanicals (mOAPBs) on the solid- and liquid-associated microenvironment (SAM and LAM, respectively) of the ruminal microbiome using an in vitro dual-flow continuous culture system. Ruminal content was incubated in eight fermenters and the basal diet was supplemented with increasing levels of mOAPBs (0; 0.12; 0.24; or 0.36% DM) which contained 55.6% hydrogenated and refined palm oil, 25% citric acid, 16.7% sorbic acid, 1.7% thymol, and 1% vanillin. All diets had a similar nutritional composition (16.1 CP, 30.9 NDF, and 32.0 starch, % DM basis). After 7 days of adaptation, a pooled sample across the days was collected in each period for identification of the microbiome of SAM and LAM. There was no effect of mOAPB on alpha-, beta-diversity, and microbial abundance. The SAM had a greater bacterial diversity and the principal component analysis demonstrated that it had a divergent bacterial profile from LAM. Additionally, SAM had an increased abundance of carbohydrate-degrading microorganisms. In summary, mOAPBs did not modulate the ruminal microbiome. The microenvironment microbiome of solid- and liquid-associated microenvironments were different, with SAM having a greater carbohydrate-degrading microorganism population. [ABSTRACT FROM AUTHOR]

Published
2023
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47. The microbiome shifts throughout the gastrointestinal tract of Bradford cattle in the Pampa biome.

Author

de Freitas, Anderson Santos, Gan, Flávia Caroline, de David, Diego Bittencourt, and Wurdig Roesch, Luiz Fernando

Subjects
WEIGHT gain, GRASSLANDS, BIOMES, CATTLE, ANIMAL breeding, GASTROINTESTINAL system, RUMEN (Ruminants), ENVIRONMENTAL degradation
Abstract

A deep understanding of the cattle gastrointestinal microbiome is crucial to selective breeding high-efficiency animals that produce more and generate less environmental damage. Here we performed the taxonomic identification of Bacterial and Archaeal communities using high throughput 16SrRNA gene sequencing from critical compartments of the gastrointestinal tract of Bradford cattle raised in a natural grassland in the Pampa biome, Brazil. We analyzed 110 samples, including saliva, ruminal fluid, and feces from 36 months old Bradford heifers (weighing on average 343 ± 30 kg by the sampling time). To reduce unexpected variation and confounders, we selected the animals from the same breed, submitted them to the same food source, and collected the samples for three consecutive years from different animals in the same season. Our main goal was to analyze the microbial shifts throughout the gastrointestinal tract to reference future works proposing management strategies and interventions to improve animal nutrition and increase production in the Pampa Biome. To accomplish our objective, we accessed the microbial community differences in groups with a high and low weight gain controlling for food ingestion and quality of grazed pasture. Few taxa were shared among the samples. About 40% of the phyla and 60% of the genera were unique from saliva samples, and 12.4% of the microbial genera were uniquely found in feces. All samples shared only 36.1% of phyla and 7.5% of genera. Differences in microbial diversity and taxa counts were observed. The ruminal fluid presented the lowest microbial richness, while saliva and feces presented the highest microbial richness. On the other hand, saliva and feces also presented more distinct communities between themselves when compared with ruminal samples. Our data showed that the saliva microbiome is not representative of the rumen microbiome and should not be used as an easy-to-collect sample for studies about the rumen microbiome. [ABSTRACT FROM AUTHOR]

Published
2022
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