1. Screening and incorporation of rust resistance from Allium cepa into bunching onion (Allium fistulosum) via alien chromosome addition
- Author
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Wako, Tadayuki, Yamashita, Ken-ichiro, Tsukazaki, Hikaru, Ohara, Takayoshi, Kojima, Akio, Yaguchi, Shigenori, Shimazaki, Satoshi, Midorikawa, Naoko, Sakai, Takako, Yamauchi, Naoki, and Shigyo, Masayoshi
- Subjects
Onions -- Genetic aspects -- Health aspects ,Plant-pathogen relationships -- Genetic aspects -- Control ,Rust fungi -- Health aspects -- Control -- Genetic aspects ,Plant immunology -- Genetic aspects ,Genetic screening -- Methods ,Biological sciences - Abstract
Bunching onion (Allium fistulosum L.; 2n = 16), bulb onion (Allium cepa L. Common onion group), and shallot (Allium cepa L. Aggregatum group) cultivars were inoculated with rust fungus, Puccinia allii, isolated from bunching onion. Bulb onions and shallots are highly resistant to rust, suggesting they would serve as useful resources for breeding rust resistant bunching onions. To identify the A cepa chromosome(s) related to rust resistance, a complete set of eight A. fistulosum--shallot monosomic alien addition lines (MAALs) were inoculated with P. allii. At the seedling stage, FF+1A showed a high level of resistance in controlled-environment experiments, suggesting that the genes related to rust resistance could be located on shallot chromosome 1A. While MAAL, multichromosome addition line, and hypoallotriploid adult plants did not exhibit strong resistance to rust. In contrast to the high resistance of shallot, the addition line FF+1A+5A showed reproducibly high levels of rust resistance. Key words: Allium fistulosum, Allium cepa, bunching onion, shallot, rust resistance, monosomic addition lines. Des cultivars de la ciboule (Allium fistulosum L.; 2n = 16), de l'oignon (Allium cepa L. groupe de l'oignon commun) et de l'echalote (Allium cepa L. groupe Aggregatum) ont ete inocules avec le champignon qui cause la rouille, Puccinia allii, isole a partir de la ciboule. Les oignons et les echalotes etaient tres resistants a la rouille, ce qui suggere qu'ils pourraient servir de source de resistance pour developper des ciboules resistantes a la rouille. Afin d'identifier le(s) chromosome(s) de l'A. cepa portant la resistance a la rouille, un jeu complet de huit lignees d'addition monosomiques (MAAL) A fistulosum - echalote ont ete inoculees avec le P. allii. Au stade plantule, la lignee FF+1A a affiche une grande resistance lors d'inoculations en conditions controlees, ce qui suggere que les genes conferant la resistance a la rouille seraient situes sur le chromosome 1A de l'echalote. Contrairement a la grande resistance presente chez l'echalote, les plantes adultes des MAAL, des lignees d'additions chromosomiques multiples ou hypoallotriploides n'affichaient pas une grande resistance a la rouille. Seule une lignee d'addition FF+1A+5A affichait une grande resistance a la rouille de maniere reproductible. [Traduit par la Redaction] Mots-cles : Allium fistulosum, Allium cepa, ciboule, echalote, esistance a la rouille, lignees d'addition monosomiques., Introduction In Japan, bunching onion (Allium fistulosum L.) is one of the most important vegetable crops and has the highest annual output (134 billion yen) of all Allium crops (Ministry [...]
- Published
- 2015
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