21 results on '"Shan, LanLan"'
Search Results
2. Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit
- Author
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Wang, Ping, Zhang, Bo, Li, Xian, Xu, Changjie, Yin, Xueren, Shan, Lanlan, Ferguson, Ian, and Chen, Kunsong
- Published
- 2010
3. Choice of surgical procedure – lobectomy, segmentectomy, or wedge resection – for patients with stage T1‐2N0M0 small cell lung cancer: A population‐based study.
- Author
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Liu, Yang, Shan, Lanlan, Shen, Jianfei, Liu, Liping, Wang, Jinlin, He, Jiaxi, He, Qihua, Jiang, Long, Guo, Minzhang, Chen, Xuewei, Zeng, Haikang, Xia, Xiaojun, Peng, Guilin, Liang, Wenhua, and He, Jianxing
- Subjects
- *
LUNG cancer prognosis , *CANCER patients , *CONFIDENCE intervals , *REPORTING of diseases , *LUNG cancer , *LUNG surgery , *MULTIVARIATE analysis , *TUMOR classification , *DECISION making in clinical medicine , *LUMPECTOMY , *PROPORTIONAL hazards models , *ODDS ratio - Abstract
Background: To date, few studies have evaluated the impact of lobectomy versus sublobar resection for early small cell lung cancer (SCLC). We investigated the survival rates of patients with pathological stage T1‐2N0M0 SCLC who underwent lobectomy or sublobar resection. Methods: We identified 548 SCLC patients in the Surveillance, Epidemiology, and End Results database who underwent lobectomy or sublobar resection. Propensity score matching (PSM) and Cox regression analysis were used to adjust for baseline characteristics. Results: The three‐year overall survival (OS) of patients treated with lobectomy (n = 376, 60%) was significantly higher than those treated with sublobar resection (n = 172, 38%). PSM and Cox multivariable analysis further confirmed this result (hazard ratio [HR] 0.543, 95% confidence interval [CI] 0.421–0.680; P < 0.001). The three‐year OS of patients treated with segmentectomy (n = 24, 54%) and wedge resection (n = 148, 36%) was not significantly different (HR 0.639, 95% CI 0.393–1.039; P = 0.071). Based on PSM analysis, segmentectomy conferred a superior survival advantage to patients relative to wedge resection (HR 0.466, 95% CI 0.221–0.979; P = 0.040). Conclusion: Lobectomy correlated with superior survival. For patients in which lobectomy is unsuitable, prognosis following segmentectomy appears to be better than after wedge resection. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
4. A rapid classification and identification method applied to the analysis of glycosides in Bupleuri radix and liquorice by ultra high performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry.
- Author
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Shan, Lanlan, Yang, Na, Zhao, Yiwei, Sheng, Xue, Yang, Shenshen, and Li, Yubo
- Subjects
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GLYCOSIDES , *LICORICE (Plant) , *HIGH performance liquid chromatography , *QUADRUPOLE moments , *PHARMACODYNAMICS , *CHINESE medicine - Abstract
Bupleuri radix and liquorice are commonly used as a hepatoprotectants. Their main effective ingredients are triterpenoid saponins. It is known that the saponins in liquorice and Bupleuri radix not only promote the metabolism of sugar and lipids but also have anti‐inflammatory functions and hepatoprotective effect. However, the complexity of these compounds results in difficulty in studying their pharmacodynamics and mechanism. In this study, glycosides were the main components that were identified and selected as the main research object. First, the mass spectrometry information of the main chemical components in liquorice and Bupleuri radix were collected from the literature. The characteristic fragments and neutral losses were summarized according to typical cleavage methods. Second, the samples were analysed using ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry, and characteristic fragment filters and neutral loss filters were successfully applied to screen 18 saponins from Bupleuri radix and 23 saponins and nine flavonoid glycosides from liquorice. Rapid classification and identification of the main components in Bupleuri radix and liquorice were finally achieved. This method promoted the development of chemical components in Bupleuri radix and liquorice, and provided a new way for screening, classifying, and identifying target components in complex samples of metabolomics and pharmacokinetics. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF
5. DEPTOR Deficiency-Mediated mTORc1 Hyperactivation in Vascular Endothelial Cells Promotes Angiogenesis.
- Author
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Ding, Yan, Shan, Lanlan, Nai, Wenqing, Lin, Xiaojun, Zhou, Ling, Dong, Xiaoying, Wu, Hongyuan, Xiao, Min, Zhou, Xuejuan, Wang, Linlin, Li, Ting, Fu, You, Lin, Yijun, Jia, Chunhong, Dai, Meng, and Bai, Xiaochun
- Subjects
- *
MTOR protein , *MTOR inhibitors , *VASCULAR endothelial cells , *NEOVASCULARIZATION , *WESTERN immunoblotting - Abstract
The mechanistic target of rapamycin (mTOR) signaling pathway is essential for angiogenesis and embryonic development. DEP domain-containing mTOR-interacting protein (DEPTOR) is an mTOR binding protein that functions to inhibit the mTOR pathwayBackground/Aims: In vitro experiments suggest that DEPTOR is crucial for vascular endothelial cell (EC) activation and angiogenic responses. However, knowledge of the effects of DEPTOR on angiogenesisin vivo is limited. This study aimed to determine the role of DEPTOR in tissue angiogenesis and to elucidate the molecular mechanisms.Methods: Cre/loxP conditional gene knockout strategy was used to delete theDeptor gene in mouse vascular ECs. The expression or distribution of cluster of differentiation 31 (CD31), vascular endothelial growth factor (VEGF) and hypoxia inducible factor-1 alpha (HIF-1α) were detected by immunohistochemical staining or western blot. Tube formation assay was used to measure angiogenesisin vitro .Results: Deptor knockdown led to increased expression of CD31, VEGF and HIF-1α in heart, liver, kidney and aorta. After treatment with rapamycin, their expression was significantly down regulated.In vitro , human umbilical vein endothelial cells (HUVECs) were transfected with DEPTOR-specific small interfering RNA (siRNA), which resulted in a significant increase in endothelial tube formation and migration rates. In contrast, DEPTOR overexpression markedly reduced the expression of CD31, VEGF and HIF-1α. Our findings demonstrated that deletion of theConclusions: Deptor gene in vascular ECs resulted in upregulated expression of CD31 and HIF-1α, and further stimulated the expression of VEGF which promoted angiogenesis, indicating that disruption of normal angiogenic pathways may occur through hyperactivation of the mTORC1/HIF-1α/VEGF signaling pathway. [ABSTRACT FROM AUTHOR]- Published
- 2018
- Full Text
- View/download PDF
6. Rapid Screening of Chemical Constituents in Rhizoma Anemarrhenae by UPLC-Q-TOF/MS Combined with Data Postprocessing Techniques.
- Author
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Shan, Lanlan, Wu, Yuanyuan, Yuan, Lei, Zhang, Yani, Xu, Yanyan, and Li, Yubo
- Subjects
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ALKALOIDS , *COMPUTERS , *FLAVONOIDS , *GLYCOSIDES , *KETONES , *LIQUID chromatography , *MASS spectrometry , *CHINESE medicine , *PLANT stems , *PHYTOCHEMICALS , *PLANT extracts , *DATA analysis - Abstract
Rhizoma Anemarrhenae, a famous traditional Chinese medicine (TCM), is the dried rhizome of Anemarrhena asphodeloides Bge. (Anemarrhena Bunge of Liliaceae). The medicine presents anti-inflammatory, antipyretic, sedative, and diuretic effects. The chemical constituents of Rhizoma Anemarrhenae are complex and diverse, mainly including steroidal saponins, flavonoids, phenylpropanoids, benzophenones, and alkaloids. In this study, UPLC-Q-TOF/MS was used in combination with data postprocessing techniques, including characteristic fragments filter and neutral loss filter, to rapidly classify and identify the five types of substances in Rhizoma Anemarrhenae. On the basis of numerous literature reviews and according to the corresponding characteristic fragments produced by different types of compounds in combination with neutral loss filtering, we summarized the fragmentation patterns of the main five types of compounds and successfully screened and identified 32 chemical constituents in Rhizoma Anemarrhenae. The components included 18 steroidal saponins, 6 flavonoids, 4 phenylpropanoids, 2 alkaloids, and 2 benzophenones. The method established in this study provided necessary data for the study on the pharmacological effects of Rhizoma Anemarrhenae and also provided the basis for the chemical analysis and quality control of TCMs to promote the development of a method for chemical research on TCMs. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
7. C4 Rice – an Ideal Arena for Systems Biology Research.
- Author
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Xin-Guang Zhu, Shan, Lanlan, Yu Wang, and Quick, William Paul
- Subjects
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CROPS , *RICE , *PHOTOSYNTHESIS , *HYPOTHESIS , *AGRICULTURAL research - Abstract
Engineering the C4 photosynthetic pathway into C3 crops has the potential to dramatically increase the yields of major C3 crops. The genetic control of features involved in C4 photosynthesis are still far from being understood; which partially explains why we have gained little success in C4 engineering thus far. Next generation sequencing techniques and other high throughput technologies are offering an unprecedented opportunity to elucidate the developmental and evolutionary processes of C4 photosynthesis. Two contrasting hypotheses about the evolution of C4 photosynthesis exist, i.e. the master switch hypothesis and the incremental gain hypothesis. These two hypotheses demand two different research strategies to proceed in parallel to maximize the success of C4 engineering. In either case, systems biology research will play pivotal roles in identifying key regulatory elements controlling development of C4 features, identifying essential biochemical and anatomical features required to achieve high photosynthetic efficiency, elucidating genetic mechanisms underlining C4 differentiation and ultimately identifying viable routes to engineer C4 rice. As a highly interdisciplinary project, the C4 rice project will have far-reaching impacts on both basic and applied research related to agriculture in the 21st century. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
8. mTOR Overactivation in Mesenchymal cells Aggravates CCl4− Induced liver Fibrosis.
- Author
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Shan, Lanlan, Ding, Yan, Fu, You, Zhou, Ling, Dong, Xiaoying, Chen, Shunzhi, Wu, Hongyuan, Nai, Wenqing, Zheng, Hang, Xu, Wanfu, Bai, Xiaochun, Jia, Chunhong, and Dai, Meng
- Abstract
Hepatic stellate cells are of mesenchymal cell type located in the space of Disse. Upon liver injury, HSCs transactivate into myofibroblasts with increase in expression of fibrillar collagen, especially collagen I and III, leading to liver fibrosis. Previous studies have shown mTOR signaling is activated during liver fibrosis. However, there is no direct evidence in vivo. The aim of this study is to examine the effects of conditional deletion of TSC1 in mesenchymal on pathogenesis of liver fibrosis. Crossing mice bearing the floxed TSC1 gene with mice harboring Col1α2-Cre-ER(T) successfully generated progeny with a conditional knockout of TSC1 (TSC1 CKO) in collagen I expressing mesenchymal cells. TSC1 CKO and WT mice were subjected to CCl4, oil or CCl4+ rapamycin treatment for 8 weeks. TSC1 CKO mice developed pronounced liver fibrosis relative to WT mice, as examined by ALT, hydroxyproline, histopathology, and profibrogenic gene. Absence of TSC1 in mesenchymal cells induced proliferation and prevented apoptosis in activated HSCs. However, there were no significant differences in oil-treated TSC1 CKO and WT mice. Rapamycin, restored these phenotypic changes by preventing myofibroblasts proliferation and enhancing their apoptosis. These findings revealed mTOR overactivation in mesenchymal cells aggravates CCl4− induced liver fibrosis and the rapamycin prevent its occurance. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
9. Corrigendum: Identification of novel genes and pathways in carotid atheroma using integrated bioinformatic methods.
- Author
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Nai, Wenqing, Threapleton, Diane, Lu, Jingbo, Zhang, Kewei, Wu, Hongyuan, Fu, You, Wang, Yuanyuan, Ou, Zejin, Shan, Lanlan, Ding, Yan, Yu, Yanlin, and Dai, Meng
- Published
- 2016
- Full Text
- View/download PDF
10. Identification of novel genes and pathways in carotid atheroma using integrated bioinformatic methods.
- Author
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Nai, Wenqing, Threapleton, Diane, Lu, Jingbo, Zhang, Kewei, Wu, Hongyuan, Fu, You, Wang, Yuanyuan, Ou, Zejin, Shan, Lanlan, Ding, Yan, Yu, Yanlin, and Dai, Meng
- Published
- 2016
- Full Text
- View/download PDF
11. Low temperature conditioning reduces postharvest chilling injury in loquat fruit
- Author
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Cai, Chong, Xu, ChangJie, Shan, LanLan, Li, Xian, Zhou, ChunHua, Zhang, WangShu, Ferguson, Ian, and Chen, KunSong
- Subjects
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WOUNDS & injuries , *FRUIT , *LOW temperatures , *LOQUAT , *COOKING - Abstract
Abstract: Chilling injury occurs in loquat (Eriobotrya japonica Lindl. cv. Luoyangqing) fruit when they are stored at temperatures lower than 5°C. In attempts to reduce this chilling injury, the effect of low temperature conditioning (LTC) was examined. Loquat fruit were conditioned at 5°C for 6 days before 0°C storage for up to 54 days. Control fruit stored at 0°C exhibited severe symptoms of lignification and tissue browning, and a decrease in percentage juice. LTC treatment significantly reduced these chilling injury symptoms, and doubled storage life. In terms of acceptability (tissue browning, internal browning (IB) index <0.4; fruit decay, <10%; flesh firmness, <6.0N; percentage juice, >60%), fruit could only be stored for 40 days at 0°C with a 3 days shelf life at 20°C, while LTC fruit could be stored for 60 days at 0°C with a similar shelf life. Similarly, LTC fruit could be stored for 40 days with a 5 days shelf life at 20°C, while fruit could be only stored for 20 days at 0°C with a 5 days shelf life. Our results confirm that LTC can effectively alleviate postharvest chilling injury of loquat fruit and may provide longer storage life with acceptable external and internal quality. [Copyright &y& Elsevier]
- Published
- 2006
- Full Text
- View/download PDF
12. Different functions of DEPTOR in modulating sensitivity to chemotherapy for esophageal squamous cell carcinoma.
- Author
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Dong, Xiaoying, Wang, Linlin, Han, Zelong, Zhou, Ling, Shan, Lanlan, Ding, Yan, Xu, Wanfu, Li, Junmeng, Su, Yongchun, Cai, Ruijun, Xiong, Gang, Diao, Dingwei, Dai, Meng, Jia, Chunhong, and Zheng, Hang
- Subjects
- *
TREATMENT of esophageal cancer , *MTOR protein , *CANCER chemotherapy , *ESOPHAGEAL cancer , *PROTEIN expression , *CANCER cells , *CELL lines , *PROGNOSIS - Abstract
There have been paradoxical findings regarding the expression of DEP domain-containing mTOR-interacting protein (DEPTOR) and its role in predicting prognosis in esophageal squamous cell carcinoma (ESCC). Here we show that DEPTOR expression was significantly increased in tumor tissues and predicted good survival in early stage ESCC patients but not in advanced stage patients. In vitro,our studies showed that ESCC cell lines could be classified into relatively high and low DEPTOR-expressing subgroups according to esophageal squamous epithelial cell line Het-1A.In our study, different levels of DEPTOR expression absolutely determined the response to chemotherapy. In relatively low-expressing cell lines, DEPTOR increased chemotherapy sensitivity via deactivation of the AKT pathway. In relatively high-expressing cell lines, DEPTOR increased cell survival and chemoresistance by strong feedback activation of the IRS1-PI3K-AKT-survivin pathway that occurred after downregulation of ribosomal protein S6 kinase (S6K). Collectively, our findings highlight the dichotomous nature of DEPTOR functions in modulating chemotherapy sensitivity in different ESCC cells. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
13. Expression of expansin genes during postharvest lignification and softening of ‘Luoyangqing’ and ‘Baisha’ loquat fruit under different storage conditions
- Author
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Yang, Shaolan, Sun, Chongde, Wang, Ping, Shan, Lanlan, Cai, Chong, Zhang, Bo, Zhang, Wangshu, Li, Xian, Ferguson, Ian, and Chen, Kunsong
- Subjects
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LOQUAT , *ERIOBOTRYA , *MESSENGER RNA , *FRUIT , *LOW temperatures - Abstract
Abstract: Four expansin cDNA fragments, EjEXPA1, EjEXPA2, EjEXPA3 and EjEXPA4, were isolated and characterized from loquat (Eriobotrya japonica Lindl.) fruit. EjEXPA1 mRNA accumulated consistently with the increase in fruit firmness in 0°C storage of ‘Luoyangqing’ (LYQ) fruit, where chilling injury with increased fruit firmness due to lignification was observed. EjEXPA1 mRNA levels were lower in fruit that underwent low temperature conditioning (LTC, 6d at 5°C then 4d at 0°C), and in 1-methylcyclopropene (1-MCP) treated fruit, in both cases where chilling injury was alleviated. Fruit of the ‘Baisha’ (BS) cultivar soften after harvest rather than increase in firmness, and high expression levels of EjEXPA1 and EjEXPA4 accompanied the softening of BS fruit stored at 20°C; such mRNA accumulation was much lower when fruit were stored at 0°C, where softening was significantly inhibited by the low temperature. Very low expression of EjEXPA2 and EjEXPA3 was observed during storage of both LYQ and BS fruit under the different storage conditions. Our results showed that of the four genes characterized, EjEXPA1 might be associated with chilling-induced lignification while both EjEXPA1 and EjEXPA4 were closely related to softening of loquat fruit during the postharvest period. [Copyright &y& Elsevier]
- Published
- 2008
- Full Text
- View/download PDF
14. Impact of examined lymph node counts on survival of patients with stage IA non-small cell lung cancer undergoing sublobar resection.
- Author
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Liu Y, Shen J, Liu L, Shan L, He J, He Q, Jiang L, Guo M, Chen X, Pan H, Peng G, Shi H, Ou L, Liang W, and He J
- Abstract
Background: The correlation between the number of examined lymph nodes (ELNs) and lung cancer-specific survival (LCSS) of stage IA non-small cell lung cancer (NSCLC) patients, who underwent sublobar resection in which lymph node (LN) sampling was relatively restricted as compared with standard lobectomy remains unclear., Methods: Patients from the Surveillance, Epidemiology, and End Results database with stage IA NSCLC who underwent sublobar resection were categorized based on ELN count (1-6 vs. ≥7; the cut point 7 was identified by Cox model)., Results: Collectively, 3,219 patients with a median follow-up time of 37 months were included in this study (G1: 1-6 ELN, n=2,410; G2: ≥7 ELN, n=809). The 5-year LCSS rate of the G1 and G2 cohorts were 75% and 83%, respectively. Cox analysis suggested that the LCSS of G1 cohort patients was lower as compared with the G2 cohort [hazard ratio (HR) =1.530; 95% confidence interval (CI): 1.240-1.988, P<0.001). Propensity score analysis also showed decreased survival of the matched G1 cohort (HR =1.499; 95% CI: 1.176-1.911; P=0.001)., Conclusions: The data suggested the ELNs ≤6 were associated with poor prognoses. Adequate LN sampling is essential even for stage IA NSCLC patients undergoing sublobar resection.
- Published
- 2018
- Full Text
- View/download PDF
15. Rapid Classification and Identification of Chemical Components of Schisandra Chinensis by UPLC-Q-TOF/MS Combined with Data Post-Processing.
- Author
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Yang S, Shan L, Luo H, Sheng X, Du J, and Li Y
- Subjects
- Chromatography, High Pressure Liquid methods, Fruit chemistry, Molecular Structure, Plant Extracts analysis, Plant Extracts chemistry, Tandem Mass Spectrometry methods, Drugs, Chinese Herbal analysis, Drugs, Chinese Herbal chemistry, Schisandra chemistry
- Abstract
Schisandra chinensis (known in Chinese as WuWeiZi, WWZ) has observable effects such as astringing the lung to stop coughs, arresting sweating, preserving semen and preventing diarrhea. The major components of WWZ include lignans, triterpenoids, organic acids and fatty acids. In this paper, a reliable method for the rapid identification of multiple components in WWZ by their characteristic fragments and neutral losses using UPLC-Q-TOF/MS technology was developed. After review of the literature and some reference experiments, the fragmentation pattern of several compounds were studied and summarized. Then, according to the corresponding characteristic fragments coupled with neutral losses in the positive or negative ion mode produced by different types of substances a rapid identification of target compounds was achieved. Finally, a total of 30 constituents of WWZ were successfully identified, including 15 lignans, nine triterpenoids, three organic acids and three fatty acids. The method established in this study not only provides a comprehensive analysis of the chemical ingredients of WWZ, providing a basis for further phytochemical studies on WWZ but also provides a more efficient way to solve the problem of identification of complex chemical constituents in traditional Chinese medicines., Competing Interests: The authors declare no conflict of interest.
- Published
- 2017
- Full Text
- View/download PDF
16. A Novel and Practical Chromatographic "Fingerprint-ROC-SVM" Strategy Applied to Quality Analysis of Traditional Chinese Medicine Injections: Using KuDieZi Injection as a Case Study.
- Author
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Yang B, Wang Y, Shan L, Zou J, Wu Y, Yang F, Zhang Y, Li Y, and Zhang Y
- Subjects
- Chromatography, High Pressure Liquid, Medicine, Chinese Traditional methods, Quality Control, Support Vector Machine, Drugs, Chinese Herbal chemistry
- Abstract
Fingerprinting is widely and commonly used in the quality control of traditional Chinese medicine (TCM) injections. However, current studies informed that the fingerprint similarity evaluation was less sensitive and easily generated false positive results. For this reason, a novel and practical chromatographic "Fingerprint-ROC-SVM" strategy was established by using KuDieZi (KDZ) injection as a case study in the present article. Firstly, the chromatographic fingerprints of KDZ injection were obtained by UPLC and the common characteristic peaks were identified with UPLC/Q-TOF-MS under the same chromatographic conditions. Then, the receiver operating characteristic (ROC) curve was used to optimize common characteristic peaks by the AUCs value greater than 0.7. Finally, a support vector machine (SVM) model, with the accuracy of 97.06%, was established by the optimized characteristic peaks and applied to monitor the quality of KDZ injection. As a result, the established model could sensitively and accurately distinguish the qualified products (QPs) with the unqualified products (UPs), high-temperature processed samples (HTPs) and high-illumination processed samples (HIPs) of KDZ injection, and the prediction accuracy was 100.00%, 93.75% and 100.00%, respectively. Furthermore, through the comparison with other chemometrics methods, the superiority of the novel analytical strategy was more prominent. It indicated that the novel and practical chromatographic "Fingerprint-ROC-SVM" strategy could be further applied to facilitate the development of the quality analysis of TCM injections., Competing Interests: The authors declare no conflict of interest.
- Published
- 2017
- Full Text
- View/download PDF
17. Casticin attenuates liver fibrosis and hepatic stellate cell activation by blocking TGF-β/Smad signaling pathway.
- Author
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Zhou L, Dong X, Wang L, Shan L, Li T, Xu W, Ding Y, Lai M, Lin X, Dai M, Bai X, Jia C, and Zheng H
- Abstract
Although many advances have been made in understanding the pathogenesis of liver fibrosis, few options are available for treatment. Casticin, one of the major flavonoids in Fructus Viticis extracts, has shown hepatoprotective potential, but its effects on liver fibrosis are not clear. In this study, we investigated the antifibrotic activity of casticin and its underlying mechanism in vivo and in vitro . Male mice were injected intraperitoneally with carbon tetrachloride (CCl
4 ) or underwent bile duct ligation (BDL) to induce liver fibrosis, followed by treatment with casticin or vehicle. In addition, transforming growth factor-β1(TGF-β1)-activated LX-2 cells were used. In vivo experiments showed that treatment with casticin alone had no toxic effect while significantly attenuating CCl4 -or BDL-induced liver fibrosis, as indicated by reductions in the density of fibrosis, hydroxyproline content, expression of α-SMA and collagen α1(I) mRNA. Moreover, casticin inhibited LX2 proliferation, induced apoptosis in a time- and dose-dependent manner in vitro . The underlying molecular mechanisms for the effect of casticin involved inhibition of hepatic stellate cell (HSC) activation and reduced the expression of matrix metalloproteinase (MMP)-2, MMP-9, tissue inhibitor of metalloproteinases (TIMP)-1 and TIMP-2 resulting from blocking TGF-β1/Smad signaling, as well as increased the apoptosis of HSCs. The results suggest that casticin has potential benefits in the attenuation and treatment of liver fibrosis., Competing Interests: CONFLICTS OF INTEREST The authors do not have any disclosures to report.- Published
- 2017
- Full Text
- View/download PDF
18. mTOR Overactivation in Mesenchymal cells Aggravates CCl 4 - Induced liver Fibrosis.
- Author
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Shan L, Ding Y, Fu Y, Zhou L, Dong X, Chen S, Wu H, Nai W, Zheng H, Xu W, Bai X, Jia C, and Dai M
- Subjects
- Animals, Disease Models, Animal, Gene Deletion, Liver pathology, Mice, Mice, Knockout, Sirolimus administration & dosage, Tuberous Sclerosis Complex 1 Protein, Tumor Suppressor Proteins genetics, Tumor Suppressor Proteins metabolism, Carbon Tetrachloride toxicity, Liver Cirrhosis chemically induced, Liver Cirrhosis pathology, TOR Serine-Threonine Kinases metabolism
- Abstract
Hepatic stellate cells are of mesenchymal cell type located in the space of Disse. Upon liver injury, HSCs transactivate into myofibroblasts with increase in expression of fibrillar collagen, especially collagen I and III, leading to liver fibrosis. Previous studies have shown mTOR signaling is activated during liver fibrosis. However, there is no direct evidence in vivo. The aim of this study is to examine the effects of conditional deletion of TSC1 in mesenchymal on pathogenesis of liver fibrosis. Crossing mice bearing the floxed TSC1 gene with mice harboring Col1α2-Cre-ER(T) successfully generated progeny with a conditional knockout of TSC1 (TSC1 CKO) in collagen I expressing mesenchymal cells. TSC1 CKO and WT mice were subjected to CCl
4 , oil or CCl4 + rapamycin treatment for 8 weeks. TSC1 CKO mice developed pronounced liver fibrosis relative to WT mice, as examined by ALT, hydroxyproline, histopathology, and profibrogenic gene. Absence of TSC1 in mesenchymal cells induced proliferation and prevented apoptosis in activated HSCs. However, there were no significant differences in oil-treated TSC1 CKO and WT mice. Rapamycin, restored these phenotypic changes by preventing myofibroblasts proliferation and enhancing their apoptosis. These findings revealed mTOR overactivation in mesenchymal cells aggravates CCl4 - induced liver fibrosis and the rapamycin prevent its occurance.- Published
- 2016
- Full Text
- View/download PDF
19. [Differential gene expression profiling for identification of potential pathogenic genes and pathways in carotid unstable plaques].
- Author
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Nai W, Liu H, Wang Y, Shan L, Fu Y, Wu H, Ding Y, Chen S, Liu Z, Chen J, and Dai M
- Subjects
- Adaptor Proteins, Signal Transducing genetics, Disease Progression, Down-Regulation, Humans, Membrane Proteins genetics, Oligonucleotide Array Sequence Analysis, Protein Interaction Maps, Real-Time Polymerase Chain Reaction, Receptors, CXCR4 genetics, Transcriptome, Up-Regulation, Vinculin genetics, Gene Expression Profiling, Plaque, Atherosclerotic genetics
- Abstract
Objective: To explore the molecular mechanism in the formation of unstable plaques., Methods: The cDNA microarray E-MTAB-2055 was downloaded from ArrayExpress database to screen the differentially expressed genes in 24 ruptured plaques against 24 stable plaques. Functional enrichment analysis was conducted to define the biological processes and pathways involved in disease progression. The protein-protein interaction network was constructed to identify the risk modules with close interactions. Five pairs of carotid specimens were used to validate 3 differentially expressed genes of the risk modules by real-time PCR., Results: A total of 439 genes showed differential expression in our analysis, including 232 up-regulated and 207 down-regulated genes according to the data filter criteria. Immune-related biological processes and pathways were greatly enriched. The protein-protein interaction network and module analysis suggested that TYROBP, VCL and CXCR4 might play critical roles in the development of unstable plaques, and differential expressions of CXCR4 and TYROBP in carotid plaques were confirmed by real-time PCR., Conclusion: Our study shows the differential gene expression profile, potential biological processes and signaling pathways involved in the process of plaque rupture. TYROBP may be a new candidate disease gene in the pathogenesis of unstable plaques.
- Published
- 2015
20. Reconstruction of gene regulatory network related to photosynthesis in Arabidopsis thaliana.
- Author
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Yu X, Zheng G, Shan L, Meng G, Vingron M, Liu Q, and Zhu XG
- Abstract
Photosynthesis is one of the most important biological processes on the earth. So far, though the molecular mechanisms underlying photosynthesis is well understood, however, the regulatory networks of photosynthesis are poorly studied. Given the current interest in improving photosynthetic efficiency for greater crop yield, elucidating the detailed regulatory networks controlling the construction and maintenance of photosynthetic machinery is not only scientifically significant but also holding great potential in agricultural application. In this study, we first identified transcription factors (TFs) related to photosynthesis through the TRAP approach using position weight matrix information. Then, for TFs related to photosynthesis, interactions between them and their targets were also determined by the ARACNE approach. Finally, a gene regulatory network was established by combining TF-targets information generated by these two approaches. Topological analysis of the regulatory network suggested that (a) the regulatory network of photosynthesis has a property of "small world"; (b) there is substantial coordination mediated by transcription factors between different components in photosynthesis.
- Published
- 2014
- Full Text
- View/download PDF
21. C4 rice - an ideal arena for systems biology research.
- Author
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Zhu XG, Shan L, Wang Y, and Quick WP
- Subjects
- Oryza genetics, Photosynthesis genetics, Photosynthesis physiology, Oryza metabolism, Systems Biology methods
- Abstract
Engineering the C4 photosynthetic pathway into C3 crops has the potential to dramatically increase the yields of major C3 crops. The genetic control of features involved in C4 photosynthesis are still far from being understood; which partially explains why we have gained little success in C4 engineering thus far. Next generation sequencing techniques and other high throughput technologies are offering an unprecedented opportunity to elucidate the developmental and evolutionary processes of C4 photosynthesis. Two contrasting hypotheses about the evolution of C4 photosynthesis exist, i.e. the master switch hypothesis and the incremental gain hypothesis. These two hypotheses demand two different research strategies to proceed in parallel to maximize the success of C4 engineering. In either case, systems biology research will play pivotal roles in identifying key regulatory elements controlling development of C4 features, identifying essential biochemical and anatomical features required to achieve high photosynthetic efficiency, elucidating genetic mechanisms underlining C4 differentiation and ultimately identifying viable routes to engineer C4 rice. As a highly interdisciplinary project, the C4 rice project will have far-reaching impacts on both basic and applied research related to agriculture in the 21st century.
- Published
- 2010
- Full Text
- View/download PDF
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