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Rhodiola rosea has been extensively used to improve physical and mental performance and to protect against stress. We, and others, have reported that R. rosea can extend lifespan in flies, worms, and yeast. However, its molecular mechanism is currently unknown. Here, we tested whether R. rosea might act through a pathway related to dietary restriction (DR) that can extend lifespan in a range of model organisms. While the mechanism of DR itself is also unknown, three molecular pathways have been associated with it: the silent information regulator 2 (SIR2) proteins, insulin and insulin-like growth factor signaling (IIS), and the target of rapamycin (TOR). In flies, DR is implemented through a reduction in dietary yeast content. We found that R. rosea extract extended lifespan in both sexes independent of the yeast content in the diet. We also found that the extract extended lifespan when the SIR2, IIS, or TOR pathways were genetically perturbed. Upon examination of water and fat content, we found that R. rosea decreased water content and elevated fat content in both sexes, but did not sensitize flies to desiccation or protect them against starvation. There were some sex-specific differences in response to R. rosea. In female flies, the expression levels of glycolytic genes and dSir2 were down-regulated, and NADH levels were decreased. In males however, R. rosea provided no protection against heat stress and had no effect on the major heat shock protein HSP70 and actually down-regulated the mitochondrial HSP22. Our findings largely rule out an elevated general resistance to stress and DR-related pathways as mechanistic candidates. The latter conclusion is especially relevant given the limited potential for DR to improve human health and lifespan, and presents R. rosea as a potential viable candidate to treat aging and age-related diseases in humans.

Immobilization of nanocatalysts on biochar is receiving unprecedented interest among material and catalysis scientists due to its simplicity, versatility, and high efficiency. Herein, we propose a new direct approach to obtain bimetallic copper/nickel nanoparticles loaded on olive stone biochar. The bimetallic-coated biochar and the reference materials, namely bare biochar, copper rich-loaded biochar, and nickel-loaded biochar, were prepared by pyrolysis from olive pit powder particles impregnated first with citric acid (CA) and then with copper and nickel nitrates at 400 °C under nitrogen flow. We employed citric acid in the process in order to examine its effect on the structural and textural properties of biochar supporting the metallic nanoparticles. Surprisingly, citric acid induced the formation of agglomerated or even raspberry-shaped bimetallic copper/nickel nanoparticles. Large 450–500 nm agglomerates of ~80 nm bimetallic CuNi NPs were noted for B-CA@CuNi. Interestingly, for biochar material prepared with initial Cu/Ni = 10 molar ratio (B-CA@CuNi10/1), the bimetallic NPs formed unusual nanoraspberries (174 ± 8 nm in size), which were agglomerates of individual 10–20 nm CuNi10/1 nanoparticles. The B-CA@CuNi and reference materials were characterized by Raman spectroscopy, scanning electron microscopy (SEM)/energy-dispersive X-ray spectroscopy (EDX), X-ray diffraction (XRD), and magnetometry. The B-CA@CuNi and B-CA@Ni materials could be efficiently attracted with a magnet but not B-CA@CuNi10/1 due to the low nickel loading. B-CA@CuNi was tested as a catalyst for the degradation of methyl orange (MO). Discoloration was noted within 10 min, much faster than a similar material prepared in the absence of CA. B-CA@CuNi could be recycled at least 3 times while still exhibiting the same fast catalytic discoloration performance. This paper stresses the important role of citric acid in shaping bimetallic nanoparticles loaded in situ on biochar during the slow pyrolysis process and in enabling faster catalytic discoloration of organic dye solution.