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Abstract Background Placenta previa accreta (PPA) is a severe obstetric condition that can cause massive postpartum hemorrhage and transfusion. Cesarean hysterectomy is necessary in some severe cases of PPA to stop the life-threatening bleeding, but cesarean hysterectomy can be associated with significant surgical blood loss and major complications. The current study is conducted to investigate the potential risk factors of excessive blood loss during cesarean hysterectomy in women with PPA. Methods This is a retrospective study including singleton pregnancies after 28 weeks of gestation in women with placenta previa and pathologically confirmed placenta accreta spectrum who received hysterectomy during cesarean sections. A total of 199 women from January 2012 to August 2023 were included in this study and were divided into Group 1 (estimated surgical blood loss (EBL) ≤ 3500 mL, n = 103) and Group 2 (EBL > 3500 mL, n = 96). The primary outcome was defined as an EBL over 3500 mL. Baseline characteristics and surgical outcomes were compared between the two groups. A multivariate logistic regression model was applied to find potential risk factors of the primary outcome. Results Massive surgical blood loss was prevalent in our study group, with a median EBL of 3500 mL. The multivariate logistic analysis showed that emergency surgery (OR 2.18, 95% CI 1.08–4.41, p = 0.029), cervical invasion of the placenta (OR 2.70, 95% CI 1.43–5.10, p = 0.002), and intraoperative bladder injury (OR 5.18, 95% CI 2.02–13.28, p = 0.001) were all associated with the primary outcome. Bilateral internal iliac arteries balloon occlusion (OR 0.57, 95% CI 0.34–0.97) and abdominal aortic balloon occlusion (OR 0.33, 95% CI 0.19–0.56) were negatively associated with the primary outcome. Conclusions Emergency surgery, cervical invasion of the placenta, and intraoperative bladder injury were potential risk factors for additional EBL during cesarean hysterectomy in women with PPA. Future prospective studies are needed to confirm the effect of intra-arterial balloon occlusion in cesarean hysterectomy of PPA.

Abstract Molecular ferroelectrics have garnered significant attention due to their structural tunability, low synthesis temperature, and high flexibility. Herein, we successfully synthesized imidazole perchlorate (ImClO4) single crystals and high-quality, highly-oriented thin films on Si substrates. These films demonstrated a high inverse piezoelectric coefficient of 55.7 pm/V. Two types of domain bands were observed: type-I bands tilted ~60° relative to the horizontal axis, and type-II bands positioned perpendicular to the horizontal axis. Under a + 20 V bias, type-I bands showed a reduction and detachment of 180° domain walls to form a needle-like domain. It extended toward the band boundary after applying −20 V bias, which grew along the boundary upon contact. In contrast, type-II bands showed straight domain wall motion and displayed a higher piezoresponse than type-I bands. The growth of high quality molecular ferroelectric thin films on Si substrates paves the way for the development of on-chip devices.

Abstract Full-Heusler alloys with earth-abundant elements exhibit high mechanical strength and favorable electrical transport behavior, but their high intrinsic lattice thermal conductivity limits potential thermoelectric application. Here, the thermoelectric transport properties of Fe-based Full-Heusler Fe2MAl (M = V, Nb, Ta) alloys are comprehensively investigated utilizing density functional theory. The results suggest that Fe2NbAl exhibits exceptionally low lattice thermal conductivity due to low phonon velocities and weakly bound Nb atoms. In Fe2NbAl, the underbonding of the Nb atoms leads large Grüneisen parameters and high anharmonic scattering rates of low-frequency acoustic phonon. Meanwhile, the high band degeneracy and large electrical conductivity lead to a maximum p-type power factor of 255.6 μW·K−2·cm−1 at 900 K. The combination of low lattice thermal conductivity and favorable electrical transport properties leads a maximum p-type dimensionless figure of merit of 1.7. Our work indicates Fe2NbAl, as a low-cost, environmentally friendly, is a potential high-performance p-type thermoelectric material.

The filled function method is a deterministic algorithm for finding a global minimizer of global optimization problems, and its effectiveness is closely related to the form of the constructed filled function. Currently, the filled functions mainly have three drawbacks in form, namely, parameter adjustment and control (if any), inclusion of exponential or logarithmic functions, and properties that are discontinuous and non-differentiable. In order to overcome these limitations, this paper proposed a parameter-free filled function that does not include exponential or logarithmic functions and is continuous and differentiable. Based on the new filled function, a filled function method for solving unconstrained global optimization problems was designed. The algorithm selected points in the feasible domain that were far from the global minimum point as initial points, and improved the setting of the step size in the stage of minimizing the filled function to enhance the algorithm's global optimization capability. In addition, tests were conducted on 14 benchmark functions and compared with existing filled function algorithms. The numerical experimental results showed that the new algorithm proposed in this paper was feasible and effective.

China’s Grain to Green Program (GTGP), which is one of the largest payments for ecosystem services (PES) in the world, has made significant ecological improvements to the environment. However, current understanding of its outcomes on the social-ecological system (SES) remains limited. Therefore, taking the South China Karst as an example, a SES resilience evaluation index system was constructed followed by an exploratory spatial analysis, root mean square error, and Self-Organizing Feature Map to clarify the spatiotemporal changes and relationship of SES resilience, achieve the zoning of SES resilience and provide restoration measures. The results showed an upward trend in social resilience from 2000 to 2020, especially its subsystem of social development. Regional ecological resilience was stable, owing to a slightly declined ecosystem services and increased landscape pattern. Spatially, nearly half of the counties exhibited a distribution mismatch in SES resilience. There was an obvious inverted U-shaped relationship of SES resilience, indicating a clear threshold effect, and the constraint relationship of SES resilience eased over time, demonstrating the effectiveness of the ecological restoration program. GTGP played a positive role in reducing regional SES trade-off, but this positive effect was limited, reflecting the limitations of overemphasizing the conversion from farmland to forest and grassland. Regional SES resilience can be divided into four clusters, which were the key optimization zone for social system, the SES resilience safety zone, the key restoration zone for SES resilience, and the key optimization zone for ecological system. Adaptive adjustments for the GTGP in these zones should be taken to achieve maximum SES benefits in the future.

IntroductionAtherosclerosis (AS) is considered the underlying cause of many diseases, particularly cardiovascular and cerebrovascular diseases. Inulin, a type of fructan, has shown potential in improving atherosclerosis, although there are conflicting findings. It is hypothesized that the polymerization degree of inulin may largely influence its therapeutic effectiveness. Therefore, this study aimed to investigate the effects and mechanisms of short-chain and long-chain inulin in AS.MethodsApoE−/− mice fed a high fat diet (HFD) were used to establish an atherosclerosis model. These mice received daily oral administration of either short-chain or long-chain inulin for 12 weeks. Plasma lipid metabolism-related indices were measured using biochemical analysis, and plasma immunological indices were analyzed via ELISA. The aorta, aortic root regions, liver tissue, adipose tissue, and colon tissue were examined through various staining techniques, including ORO staining, hematoxylin and eosin staining, Alcian blue staining, and immunofluorescent or immunohistochemical assays. Microbiome analysis was conducted in the cecal content.ResultsThe results indicated that both short-chain and long-chain inulin substantially reduced the formation of atherosclerotic plaques. Inulin also improved plasma lipid concentrations and hepatic lipid metabolism, and partially alleviated both localized (atherosclerotic lesions) and systemic inflammation. Short-chain inulin was more effective than long-chain inulin in reducing atherosclerotic plaques formation, enhancing lipid metabolism and reducing inflammation. Additionally, both types of inulin showed similar effectiveness in enhancing intestinal epithelial barrier integrity, gut microbiota composition and functionality.ConclusionThese findings suggest that inulin has a protective role against atherosclerosis by enhancing lipid metabolism, reducing inflammation, and improving intestinal barrier and gut microbiota. As a dietary intervention, short-chain inulin is more effective than long-chain inulin, offering clinical implications for using inulin as a therapeutic agent for atherosclerosis.

The formation of continental red beds is generally considered to be related to an arid climate. Heating experiments (performed by L.J. and G.C.) using dried black mud sediment also demonstrate that the reddening may be caused by the transformation of goethite to haematite that begins at approximately 150 °C under anhydrous conditions, and increasing the temperature to 450 °C is positively correlated with the red colour and peak value of haematite. If this process applies to continental red beds, it implies a thermal origin of red beds as a result of high diagenetic temperatures rather than as the cause of their deposition under an arid climate. Namely, subsiding red-bed basins are heated from below rather than warmed from above. Here, we further strengthen this idea by new evidence from borehole cores drilled from red beds in SE China, showing a clear geological section from the surface soil to red beds to bottom granite. The data reveal that the continental red beds formed at least at a temperature within 150–400 °C, and the underlying granite usually formed at temperatures greater than 600 °C. Our results imply a possible relationship between continental red bed events and Earth's thermal cycles.

Ideal medical implant materials should have the qualities of osteoinduction, osteoconduction, nontoxicity, non-sensitivity and non-carcinogenicity to the body. Although its high melting point and high elastic modulus pose difficulties for processing technology, tantalum has become a popular research topic in the field of medical implant materials because of its excellent biocompatibility and bone inductivity. In this paper, the performance of the bone-inducing properties of tantalum in different applications, including its combination with other elements, its use as a coating, its porous structure and its surface modification ability, are summarized. The mechanism of promoting bone formation and the clinical application of tantalum are reviewed.

Abstract Background Lipids found in plant seeds are essential for controlling seed dormancy, dispersal, and defenses against biotic and abiotic stress. Additionally, these lipids provide nutrition and energy and are therefore important to the human diet as edible oils. Acer truncatum, which belongs to the Aceaceae family, is widely cultivated around the world for its ornamental value. Further because its seed oil is rich in unsaturated fatty acids (UFAs)- i.e. α-linolenic acid (ALA) and nervonic acid (NA)- and because it has been validated as a new food resource in China, the importance of A. truncatum has greatly risen. However, it remains unknown how UFAs are biosynthesized during the growth season, to what extent environmental factors impact their content, and what areas are potentially optimal for their production. Results In this study, transcriptome and metabolome of A. truncatum seeds at three representative developmental stages was used to find the accumulation patterns of all major FAs. Cumulatively, 966 metabolites and 87,343 unigenes were detected; the differential expressed unigenes and metabolites were compared between stages as follows: stage 1 vs. 2, stage 1 vs. 3, and stage 2 vs. 3 seeds, respectively. Moreover, 13 fatty acid desaturases (FADs) and 20 β-ketoacyl-CoA synthases (KCSs) were identified, among which the expression level of FAD3 (Cluster-7222.41455) and KCS20 (Cluster-7222.40643) were consistent with the metabolic results of ALA and NA, respectively. Upon analysis of the geographical origin-affected diversity from 17 various locations, we found significant variation in phenotypes and UFA content. Notably, in this study we found that 7 bioclimatic variables showed considerable influence on FAs contents in A. truncatum seeds oil, suggesting their significance as critical environmental parameters. Ultimately, we developed a model for potentially ecological suitable regions in China. Conclusion This study provides a comprehensive understanding of the relationship between metabolome and transcriptome in A. truncatum at various developmental stages of seeds and a new strategy to enhance seed FA content, especially ALA and NA. This is particularly significant in meeting the increasing demands for high-quality edible oil for human consumption. The study offers a scientific basis for A. truncatum’s novel utilization as a woody vegetable oil rather than an ornamental plant, potentially expanding its cultivation worldwide.

Abstract Although adverse environmental exposures are considered a major cause of chronic diseases, current studies provide limited information on real-world chemical exposures and related risks. For this study, we collected serum samples from 5696 healthy people and patients, including those with 12 chronic diseases, in China and completed serum biomonitoring including 267 chemicals via gas and liquid chromatography-tandem mass spectrometry. Seventy-four highly frequently detected exposures were used for exposure characterization and risk analysis. The results show that region is the most critical factor influencing human exposure levels, followed by age. Organochlorine pesticides and perfluoroalkyl substances are associated with multiple chronic diseases, and some of them exceed safe ranges. Multi-exposure models reveal significant risk effects of exposure on hyperlipidemia, metabolic syndrome and hyperuricemia. Overall, this study provides a comprehensive human serum exposome atlas and disease risk information, which can guide subsequent in-depth cause-and-effect studies between environmental exposures and human health.

Abstract Sugar Will Eventually be Exported Transporter (SWEET) proteins are highly conserved in various organisms and play crucial roles in sugar transport processes. However, SWEET proteins in peanuts, an essential leguminous crop worldwide, remain lacking in systematic characterization. Here, we identified 94 SWEET genes encoding the conservative MtN3/saliva domains in three peanut species, including 47 in Arachis hypogea, 23 in Arachis duranensis, and 24 in Arachis ipaensis. We observed significant variations in the exon-intron structure of these genes, while the motifs and domain structures remained highly conserved. Phylogenetic analysis enabled us to categorize the predicted 286 SWEET proteins from eleven species into seven distinct groups. Whole genome duplication/segment duplication and tandem duplication were the primary mechanisms contributing to the expansion of the total number of SWEET genes. In addition, an investigation of cis-elements in the potential promoter regions and expression profiles across 22 samples uncovered the diverse expression patterns of AhSWEET genes in peanuts. AhSWEET24, with the highest expression level in seeds from A. hypogaea Tifrunner, was observed to be localized on both the plasma membrane and endoplasmic reticulum membrane. Moreover, qRT-PCR results suggested that twelve seed-expressed AhSWEET genes were important in the regulation of seed development across four different peanut varieties. Together, our results provide a foundational basis for future investigations into the functions of SWEET genes in peanuts, especially in the process of seed development.

Less research has been conducted on the association between camellia oil’s (CO) phenolic composition and antioxidant capability. In this study, the phenolic profile of CO and its connection to antioxidant capacity were examined utilizing a combination of widely-targeted phenolic metabolomics and multivariate statistical analysis. A total of 751 phenolics were discovered. The WGCNA was used to link phenols to antioxidants, yielding 161 antioxidant-related phenols from the blue module. In response to several antioxidant assays, 59 (FRAP), 59 (DPPH), and 53 (ABTS) phenolics were identified as differential phenolic markers (DPMs). Further stepwise multiple linear regression revealed six DPMs that substantially influenced the antioxidant capacities. Nine metabolic pathways and their associated network mechanisms for the most significant phenolics were developed. This study sheds light on the phenolic content of CO, elucidates their role in antioxidant activity, and lays the groundwork for improving extraction techniques and generating improved product.

Rydberg atom receiver attracts much research interest for promising applications in communications and sensing. Generally, Rydberg atomic receiver is utilized with microwave frequency comb (MFC) to expand its detectable frequency range. MFC consists of a set of equally spaced discrete frequency lines, resembling a comb in frequency space. In such receives, the mixing of RF signal and its closest MFC component excites the atoms into the desired Rydberg state. Since the mixing rather than RF signal is detected, there exists inevitable ambiguity of frequency estimation. In this paper, we provide a novel frequency ambiguity resolution based on improved Chinese remainder theorem (I-CRT). It realizes the instantaneous frequency estimation of RF signal with the MFC-based Rydberg atomic receiver. The effectiveness of proposed resolution in this paper is verified by both simulation experiments and theoretical analysis.

Previous studies have confirmed the time-lagged and cumulative effects of drought and anthropogenic activities on vegetation growth, but these studies focus on the time-lagged effect of drought and are poorly known how vegetation productivity responds to anthropogenic activities. Here, based on the reconstructed Normalized Difference Vegetation Index, the Standardized Precipitation Evapotranspiration Index and land use degree comprehensive index, we diagnosed the spatiotemporal pattern of vegetation and drought, investigated time-lagged and cumulative effects of drought and anthropogenic activities over China through the month where the maximum correlation coefficient occurred. It revealed that the browning trend of 32.21% of vegetated lands was covered by overall greening, especially in northwestern China. Drought intensified with a rate of 0.0014/year. 66.41% and 54.57% of the vegetated lands had time-lagged and cumulative response to drought, with a shorter timescales of 1–4 months, indicating the higher sensitivity of vegetation growth to drought. There was a U-shaped relationship between moisture conditions and vegetation response time. 49.9% of China's vegetation showed time-lagged effects to anthropogenic activities, with a longer timescales of 6–10 years, demonstrating that anthropogenic activities triggered ecological changes but vegetation ecosystems cannot keep pace. The accumulated and time-lagged years declined with increased land use intensity.

Fungal laccase has strong ability in detoxification of many environmental contaminants. A putative laccase gene, LeLac12, from Lentinula edodes was screened by secretome approach. LeLac12 was heterogeneously expressed and purified to characterize its enzymatic properties to evaluate its potential use in bioremediation. This study showed that the extracellular fungal laccase from L. edodes could effectively degrade tetracycline (TET) and the synthetic dye Acid Green 25 (AG). The growth inhibition of Escherichia coli and Bacillus subtilis by TET revealed that the antimicrobial activity was significantly reduced after treatment with the laccase-HBT system. 16 transformation products of TET were identified by UPLC-MS-TOF during the laccase-HBT oxidation process. Gas chromatography-mass spectrometry (GC-MS) analysis revealed that LeLac12 could completely mineralize ring-cleavage products. LeLac12 completely catalyzed 50 mg/L TET within 4 h by adding AG (200 mg/L), while the degradation of AG was above 96% even in the co-contamination system. Proteomic analysis revealed that central carbon metabolism, energy metabolism, and DNA replication/repair were affected by TET treatment and the latter system could contribute to the formation of multidrug-resistant strains. The results demonstrate that LeLac12 is an efficient and environmentally method for the removal of antibiotics and dyes in the complex polluted wastewater.

Skin cancer is threatening more and more people’s health; its postoperative recurrence and wound infection are still critical challenges. Therefore, specialty wound dressings with multifunctional bioactivity are urgently desired. Emodin is a natural anthraquinone compound that has anti-cancer and anti-bacterial properties. Herein, we fabricated coaxial electrospinning nanofibers loaded with emodin to exploit a multifunctional wound dressing for skin cancer postoperative management, which encapsulated emodin in a polyvinylpyrrolidone core layer, combined with chitosan-polycaprolactone as a shell layer. The nanofibers were characterized via morphology, physicochemical nature, drug load efficiency, pH-dependent drug release profiles, and biocompatibility. Meanwhile, the anti-cancer and anti-bacterial effects were evaluated in vitro. The emodin-loaded nanofibers exhibited smooth surfaces with a relatively uniform diameter distribution and a clear shell-core structure; remarkably, emodin was evenly dispersed in the nanofibers with significantly enhanced dissolution of emodin. Furthermore, they not only display good wettability, high emodin entrapment efficiency, and biphasic release profile but also present superior biocompatibility and anti-cancer properties by increasing the levels of MDA and ROS in A-375 and HSC-1 cells via apoptosis-related pathway, and long-term anti-bacterial effects in a dose-independent manner. The findings indicate that the emodin-loaded nanofiber wound dressing can provide a potential treatment strategy for skin cancer postoperative management.

Mango (Mangifera indica) is a highly valuable horticultural crop known for its quality and productivity. This study investigates the dynamic changes in physicochemical properties and glutathione and ascorbic acid metabolic pathways in mango pulp across various growth and development stages over two consecutive years (2021–2022 and 2022–2023) by transcriptomic analysis. Overall, the results demonstrate that during different ripening periods, the pulp shows increased levels of total soluble solids, relative conductivity, glutathione, and enzymes, while titratable acidity, malondialdehyde, reactive oxygen species, and ascorbic acid contents decreased. Moreover, transcriptomic analysis identified key differentially expressed genes from the glutathione and ascorbic acid metabolic pathways and validated them with qRT-PCR. In different comparisons, a total of 1776, 2513, and 828 DEGs were identified in 30 vs. 60, 30 vs. 90, and 60 vs. 90 days after flowering, respectively. Among them, seven DEGs were primarily enriched in relevant pathways, which included ascorbate peroxidase, ascorbate oxidase, glutathione peroxidase, gamma-glutamyl transferase, glutathione transferases, and glucose-6-phosphate dehydrogenase. The upregulation of these genes indicates that glutathione and AsA respond well to scavenging reactive oxygen species and maintain normal functioning in plants. This research sheds light on the molecular mechanisms of glutathione and ascorbic acid dynamic changes in mango pulp, providing valuable insights into the regulation of antioxidant and metabolic pathways during fruit growth and development.

Aim: The aim of this study is to comprehensively investigate the distribution of amine and phenol compounds in different flavors of Baijiu. Methods: 12C-/13C-dansyl chloride labeling was applied for untargeted and quantitative analyses of amine and phenol compounds in Baijiu. Results: A total of 267 amine/phenol compounds were detected, and 30 of them were confirmed by the standards. 4 of 30 confirmed compounds were newly identified in Baijiu, and 16 ones were related with flavor or biological activity. After statistical analysis, 34 amine/phenol compounds were defined as potential markers for indicating sauce flavor, strong flavor, and light flavor Baijiu. 30 compounds in Baijiu were quantified with high precision, high accuracy, and high sensitivity. Results of the untargeted and quantitative analyses indicated that the number and contents of amine and phenol compounds were generally richest in sauce flavor Baijiu, while lowest in light flavor Baijiu. Conclusions: The results obtained in the research are beneficial for comprehensively understanding the amine and phenol compounds in Baijiu and further provide the basis for the flavor blending of Baijiu.

Pre-harvest sprouting (PHS) is one of the serious global issues in wheat production. Identification of quantitative trait loci (QTL) and closely-linked markers is greatly helpful for wheat improvement. In the present study, a recombinant inbred line (RIL) population derived from the cross of Zhongmai 578 (ZM578)/Jimai 22 (JM22) and parents were phenotyped in five environments and genotyped by the wheat 50 K single-nucleotide polymorphism (SNP) array. Two QTL of germination index (GI), QGI.caas-3A and QGI.caas-5A, were detected, explaining 4.33%–5.58% and 4.43%–8.02% of the phenotypic variances, respectively. The resistant effect of QGI.caas-3A was contributed by JM22, whereas that of QGI.caas.5A was from ZM578. The two QTL did not correspond to any previously identified genes or genetic loci for PHS-related traits according to their locations in the Chinese Spring reference genome, indicating that they are likely to be new loci for PHS resistance. Four kompetitive allele-specific PCR (KASP) markers K_AX-109605367and K_AX-179559687 flanking QGI.caas-3A, and K_AX-111258240 and K_AX-109402944 flanking QGI.caas-5A, were developed and validated in a natural population of 100 wheat cultivars. The distribution frequency of resistance alleles at Qphs.caas-3A and Qphs.caas-5A loci were 82.7% and 57.1%, respectively, in the natural population. These findings provide new QTL and tightly linked KASP markers for improvement of PHS resistance in wheat.

Rumination in cattle is closely related to their health, which makes the automatic monitoring of rumination an important part of smart pasture operations. However, manual monitoring of cattle rumination is laborious and wearable sensors are often harmful to animals. Thus, we propose a computer vision-based method to automatically identify multi-object cattle rumination, and to calculate the rumination time and number of chews for each cow. The heads of the cattle in the video were initially tracked with a multi-object tracking algorithm, which combined the You Only Look Once (YOLO) algorithm with the kernelized correlation filter (KCF). Images of the head of each cow were saved at a fixed size, and numbered. Then, a rumination recognition algorithm was constructed with parameters obtained using the frame difference method, and rumination time and number of chews were calculated. The rumination recognition algorithm was used to analyze the head image of each cow to automatically detect multi-object cattle rumination. To verify the feasibility of this method, the algorithm was tested on multi-object cattle rumination videos, and the results were compared with the results produced by human observation. The experimental results showed that the average error in rumination time was 5.902% and the average error in the number of chews was 8.126%. The rumination identification and calculation of rumination information only need to be performed by computers automatically with no manual intervention. It could provide a new contactless rumination identification method for multi-cattle, which provided technical support for smart pasture.

Abstract Background The research aimed to evaluate the optimal Single-Photon Emission Computed Tomography (SPECT) cut-off value in differentiating condylar growth activeness, to observe 3-dimensional (3D) mandibular growth pattern, and to explore the potential correlation between 3D measurement parameters and SPECT uptake ratios in Chinese unilateral condylar hyperplasia (UCH) patients. Methods Data of fifty-four Chinese UCH patients were analyzed retrospectively. All patients underwent SPECT within 1 month before or after the first CT examination (CT1); and received a second CT examination at least 12 months later (CT2). Data from CT scans were analyzed by comparing bilateral differences between CT1 and CT2. The sensitivity and specificity of SPECT were calculated by the receiver operating characteristic (ROC) curve. Pearson’s correlation analysis was performed to investigate whether the mandibular growth was correlated with SPECT value. Results SPECT had a sensitivity of 68.00% and a specificity of 72.41%, with an area under the ROC curve being 0.709. The optimal SPECT cut-off value for evaluating condylar activity has been determined to be 13%. In patients with an active growing condyle, there was a significant increase in Co-Gn and Co-Go, but not in Go-Gn, Go-MF, or MF-Gn. Pearson’s correlation analysis revealed no correlation between 3D measurement parameters and differences in relative condylar uptake ratios. Conclusion SPECT showed good diagnostic performance in UCH with the cut-off value of 13%. For those with an active growing condyle, the mandible grows diagonally and vertically, while the relative condylar uptake ratio was not directly related to mandibular growth.

Alzheimer’s disease (AD), a primary cause of dementia, is rapidly emerging as one of the most financially taxing, lethal, and burdensome diseases of the 21st century. Increasing evidence suggests that microglia-mediated neuroinflammation plays a key role in both the initiation and progression of AD. Recently, emerging evidence has demonstrated mitochondrial dysfunction, particular in microglia where precedes neuroinflammation in AD. Multiple signaling pathways are implicated in this process and pharmaceutical interventions are potentially involved in AD treatment. In this review, advance over the last five years in the signaling pathways and pharmaceutical interventions are summarized and it is proposed that targeting the signaling pathways in microglia with mitochondrial dysfunction could represent a novel direction for AD treatment.

Ecosystem carbon use efficiency (CUE), water use efficiency (WUE), and light use efficiency (LUE) are critical parameters for estimating CO2 uptake, water circulation, and ecosystem balance. Research on the change trends of individual use efficiency has matured; however, studies on the spatiotemporal heterogeneity and driving mechanisms of divergence patterns for multi-use efficiencies capability are limited. Therefore, taking southwest China as an example, this study constructed a compound use efficiency (COM) through CUE, WUE, and LUE. Based on the spatiotemporal patterns and divergence characteristics analysis of water–carbon–light use efficiencies, the scale effects and driving mechanism of its divergence characteristics for COM at the optimal scale were clarified. The results revealed that the average value of CUE, LUE, WUE, and COM were 0.49, 0.7 gC m−2 MJ−1, 2.31 gC kg−1 H2O, and 0.87, respectively. Apart from CUE, the LUE, WUE, and COM parameters exhibited a fluctuating upward trend. Statistically, there was a high COM in karst and ecological restoration regions, reflecting the strong adaptability of karst vegetation and the effectiveness of ecological restoration; as the elevation rose, COM increased and then decreased, with the highest value at the elevation of 3000 m; the lowest COM was found in grassland, refuting the inference that it can be used as an optimal vegetation type for China’s Grain to Green program from the perspective of use efficiency. Sub-basin was the most optimal divergence scale, and although temperature and elevation were the dominant single force causing COM divergence, the couplings of precipitation and population density and elevation and population density had more controlling impacts than a single force. These findings enrich the understanding of ecosystem use efficiency and are beneficial for the improvement in ecological restoration strategies in karst landscapes.

Summary: We identified that the genes heat shock transcription factor 5 (hsf5) and ring finger protein 43 (rnf43) happened fusion in Nile tilapia (Oreochromis niloticus), called hsf5-rnf43, and provided the characteristic and functional analysis of hsf5-rnf43 gene in fish for the first time. Analysis of spatiotemporal expression showed that hsf5-rnf43 was specifically expressed in the testis and located in primary spermatocytes of adult Nile tilapia and gradually increased during testis development from 5 to 180 days after hatching. We also found DNA methylation regulated sex-biased expression of hsf5-rnf43 in the early development of Nile tilapia, and was affected by high temperature during the thermosensitive period of Nile tilapia sex differentiation. Therefore, we first reported that the fusion gene hsf5-rnf43 was sex-biased expressed in the testis regulated by DNA methylation and affected by high temperature, which may be involved in the maintenance of testis function and sex differentiation of Nile tilapia.

Soybean (Glycine max (L.) Merr.) is a globally significant crop, widely cultivated for oilseed production and animal feeds. In recent years, the rapid growth of multi-omics data from thousands of soybean accessions has provided unprecedented opportunities for researchers to explore genomes, genetic variations, and gene functions. To facilitate the utilization of these abundant data for soybean breeding and genetic improvement, the SoybeanGDB database (https://venyao.xyz/SoybeanGDB/) was developed as a comprehensive platform. SoybeanGDB integrates high-quality de novo assemblies of 39 soybean genomes and genomic variations among thousands of soybean accessions. Genomic information and variations in user-specified genomic regions can be searched and downloaded from SoybeanGDB, in a user-friendly manner. To facilitate research on genetic resources and elucidate the biological significance of genes, SoybeanGDB also incorporates a variety of bioinformatics analysis modules with graphical interfaces, such as linkage disequilibrium analysis, nucleotide diversity analysis, allele frequency analysis, gene expression analysis, primer design, gene set enrichment analysis, etc. In summary, SoybeanGDB is an essential and valuable resource that provides an open and free platform to accelerate global soybean research.

Ten 16-year-old trees were used as test materials to investigate the effect of foliar calcium fertilizer on the sugar content of ‘Feizixiao’ litchi (Litchi chinensis Sonn.) pulp. The experiment began 35 days after anthesis (DAA) in 2020 and 2021, and the treatment was a foliar spray application of 0.3% CaCl2 aqueous solution, whereas the control was a foliar spray application of water. The sugar content, sucrose-metabolizing enzymes, and ATP-dependent phosphofructokinase (PFK) activities in pulp were measured in 2020 and 2021. Transcriptome sequencing (RNA-seq) was performed on RNA samples from treatment and control fruit pulps at 35, 63, and 69 DAA (full mature stage) in 2020, and 10 genes were chosen for confirmation by real-time polymerase chain reaction (PCR) in 2020 and 2021. At full maturity, the soluble sugar content in the calcium-treated group was extremely significantly or significantly higher than that in the control group. After 63 DAA, the net sucrose-metabolizing enzyme activity in the calcium-treated group was significantly higher than that in the control group. Furthermore, at full maturity, the calcium-treated group had significantly higher sucrose synthase cleavage activity and significantly lower PFK activity than the control group. Fifty-four highly expressed genes in the glycolytic pathway (EMP) were screened from transcriptome data, including hexokinase, PFK, and pyruvate kinase genes; 87% of these genes were downregulated in the treatment group compared with the control group at 69 DAA in 2020. The linear regression between RNA-seq and real-time PCR results was significant in 2020 (r = 0.9292) and 2021 (r = 0.8889). When the fruit is fully ripe, calcium treatment increases net sucrose-metabolizing enzyme activity by increasing sucrose synthase cleavage activity, promoting the accumulation of reducing sugars, and it downregulates phosphofructokinase gene expression in EMP, promoting sugar accumulation.

Considering that the mechanical mechanism and energy release law of gradual failure of columnar jointed basalt (CJB) are affected by lateral pressure, rock heterogeneity and column irregularity, etc., the numerical CJB models with various column dip angles, irregularity degrees of column and lateral pressures were established through the digital image correlation. The progressive failure process and released energy of CJBs were simulated under the loading rate of 0.05 mm/step based the continuum mechanics and statistical damage theory. The influences of rock inhomogeneity, column irregularity degree, and model boundary on the nonlinear deformation and failure of CJB were comprehensively investigated. The results demonstrate that the effect of the inhomogeneity index depends on column dip angle, and the equivalent deformation modulus of CJBs is more sensitive to the inhomogeneity index than the lateral pressure. Moreover, the compressive strength along the direction perpendicular to the column axis basically decreases with column irregularity degree increasing when the meso strength of rock is 120 MPa. Besides, the mechanical properties and elastic energy of irregular CJBs are greatly affected by the model boundaries. These achievements will contribute to understanding the inner failure mechanism and energy evolution of CJBs.

Purpose – Perception has been identified as the main cause underlying most autonomous vehicle related accidents. As the key technology in perception, deep learning (DL) based computer vision models are generally considered to be black boxes due to poor interpretability. These have exacerbated user distrust and further forestalled their widespread deployment in practical usage. This paper aims to develop explainable DL models for autonomous driving by jointly predicting potential driving actions with corresponding explanations. The explainable DL models can not only boost user trust in autonomy but also serve as a diagnostic approach to identify any model deficiencies or limitations during the system development phase. Design/methodology/approach – This paper proposes an explainable end-to-end autonomous driving system based on “Transformer,” a state-of-the-art self-attention (SA) based model. The model maps visual features from images collected by onboard cameras to guide potential driving actions with corresponding explanations, and aims to achieve soft attention over the image’s global features. Findings – The results demonstrate the efficacy of the proposed model as it exhibits superior performance (in terms of correct prediction of actions and explanations) compared to the benchmark model by a significant margin with much lower computational cost on a public data set (BDD-OIA). From the ablation studies, the proposed SA module also outperforms other attention mechanisms in feature fusion and can generate meaningful representations for downstream prediction. Originality/value – In the contexts of situational awareness and driver assistance, the proposed model can perform as a driving alarm system for both human-driven vehicles and autonomous vehicles because it is capable of quickly understanding/characterizing the environment and identifying any infeasible driving actions. In addition, the extra explanation head of the proposed model provides an extra channel for sanity checks to guarantee that the model learns the ideal causal relationships. This provision is critical in the development of autonomous systems.

Abstract Humans and Acanthamoeba polyphaga mimivirus share numerous homologous genes, including collagens and collagen-modifying enzymes. To explore this homology, we performed a genome-wide comparison between human and mimivirus using DELTA-BLAST (Domain Enhanced Lookup Time Accelerated BLAST) and identified 52 new putative mimiviral proteins that are homologous with human proteins. To gain functional insights into mimiviral proteins, their human protein homologs were organized into Gene Ontology (GO) and REACTOME pathways to build a functional network. Collagen and collagen-modifying enzymes form the largest subnetwork with most nodes. Further analysis of this subnetwork identified a putative collagen glycosyltransferase R699. Protein expression test suggested that R699 is highly expressed in Escherichia coli, unlike the human collagen-modifying enzymes. Enzymatic activity assay and mass spectrometric analyses showed that R699 catalyzes the glucosylation of galactosylhydroxylysine to glucosylgalactosylhydroxylysine on collagen using uridine diphosphate glucose (UDP-glucose) but no other UDP-sugars as a sugar donor, suggesting R699 is a mimiviral collagen galactosylhydroxylysyl glucosyltransferase (GGT). To facilitate further analysis of human and mimiviral homologous proteins, we presented an interactive and searchable genome-wide comparison website for quickly browsing human and Acanthamoeba polyphaga mimivirus homologs, which is available at RRID Resource ID: SCR_022140 or https://guolab.shinyapps.io/app-mimivirus-publication/ .

In this paper, we consider the two dimensional incompressible anisotropic magneto-micropolar fluid equations with partial mixed velocity dissipations, magnetic diffusion and horizontal vortex viscosity, and analyze the stability near a background magnetic field. At present, major works on the equations of magneto-micropolar fluid mainly focus on the global regularity of the solutions. While the stability of the solutions remains an open problem. This paper concentrates on establishing the stability for the linear and nonlinear system respectively. Two goals have been achieved. The first is to obtain the explicit decay rates for the solution of the linear system in $ H^s({\mathbb{R}}^2) $ Sobolev space. The second assesses the nonlinear stability by establishing the a priori estimate and employing bootstrapping arguments. Our results reveal that any perturbations near a background magnetic field is globally stable in Sobolev space $ H^2({\mathbb{R}}^2) $.

Neuroinflammation is considered to have a prominent role in the pathogenesis of Alzheimer’s disease (AD). Microglia are the resident macrophages of the central nervous system, and modulating microglia activation is a promising strategy to prevent AD. Essential oil of Jasminum grandiflorum L. flowers is commonly used in folk medicine for the relief of mental pressure and disorders, and analyzing the volatile compound profiles and evaluating the inhibitory effects of J. grandiflorum L. essential oil (JGEO) on the excessive activation of microglia are valuable for its application. This study aims to explore the potential active compounds in JGEO for treating AD by inhibiting microglia activation-integrated network pharmacology, molecular docking, and the microglia model. A headspace solid-phase microextraction combined with the gas chromatography–mass spectrometry procedure was used to analyze the volatile characteristics of the compounds in J. grandiflorum L. flowers at 50°C, 70°C, 90°C, and 100°C for 50 min, respectively. A network pharmacological analysis and molecular docking were used to predict the key compounds, key targets, and binding energies based on the detected compounds in JGEO. In the lipopolysaccharide (LPS)-induced BV-2 cell model, the cells were treated with 100 ng/mL of LPS and JGEO at 7.5, 15.0, and 30 μg/mL, and then, the morphological changes, the production of nitric oxide (NO) and reactive oxygen species, and the expressions of tumor necrosis factor-α, interleukin-1β, and ionized calcium-binding adapter molecule 1 of BV-2 cells were analyzed. A total of 34 compounds with significantly different volatilities were identified. α-Hexylcinnamaldehyde, nerolidol, hexahydrofarnesyl acetone, dodecanal, and decanal were predicted as the top five key compounds, and SRC, EGFR, VEGFA, HSP90AA1, and ESR1 were the top five key targets. In addition, the binding energies between them were less than −3.9 kcal/mol. BV-2 cells were activated by LPS with morphological changes, and JGEO not only could clearly reverse the changes but also significantly inhibited the production of NO and reactive oxygen species and suppressed the expressions of tumor necrosis factor-α, interleukin-1β, and ionized calcium-binding adapter molecule 1. The findings indicate that JGEO could inhibit the overactivation of microglia characterized by decreasing the neuroinflammatory and oxidative stress responses through the multi-compound and multi-target action modes, which support the traditional use of JGEO in treating neuroinflammation-related disorders.

Progress toward developing next-generation biomaterials applied in complex bone defect is expected to effectively integrate osteogenic and anti-bacteria capability. However, current biomaterials often achieve the two functions separately. Inspired by photothermal effect, exogenous light-induced thermal variations can efficiently facilitate bone regeneration and bacteria eradication. Here, a bioactive hydrogel BMP-2@PNH-TA with simultaneously osteogenic and anti-bacterial capacities is presented, which is based on the near infrared (NIR) induced photothermal effect to perform integrated photothermal therapy (PTT). In detail, photothermal effect and photothermal-controlled release of osteogenic and anti-bacterial factors through sponge-like effect could exert integrated influence on bone regeneration under complex situation. The composite hydrogel can mediate pro-osteogenesis under mild temperature and controlled release of BMP-2 and tannin in response to NIR stimulation with low power, while efficiently eradicate bacteria via controlled release of O-HACC and thermal effect with high power. The customized PTT strategy significantly shortened the duration time of bone regeneration up to almost 50% in rat cranial bone defect. It is anticipated that the concept of integrated PTT for bone regeneration will cast new insights into developing high-performance therapeutic strategy with combined biological functions.

Extensive studies have demonstrated the spatiotemporal changes in carbon use efficiency (CUE) and its driving factors over the past three decades. However, how the global CUE will change and to what extent the CUE is affected by the dominant factor in this century is still unclear. Herein, based on CMIP6 model outputs, we estimated the situation and change trends of CUE in baseline (1982–2014) and future (2015–2100), and identified the controlling factor of CUE variation by boosted regression tree. Further, we predicted the CUE-controlling factor sensitivity ( S _value , referring to higher/lower controlling factor producing more/less CUE) and its variation under four representative pathways, and revealed the relationship between S _value and social economy. Results showed decreased CUE at the end of the 21st century, especially in the SSP5-8.5, its decline rate of CUE is 1.2 × 10 ^−2 ± 5.2 × 10 ^−4 /decade, which is 10 times higher than that in the SSP1-2.6. Spatially, 56.9%, 74.5%, 83.1%, and 88.6% of the global land will exhibit a decreased CUE under the SSP1-2.6, SSP2-4.5, SSP3-7.0, and SSP5-8.5 scenarios, and primarily concentrates at the middle-high latitudes of the Northern Hemisphere (30°–60° N). Except in Africa, temperature is the controlling factor for CUE variation, and the S _value decreases over time, indicating an enhanced inhibitory effect of temperature on CUE. The turning time of S _value change will advance with increases in CO _2 emission, presenting prolonged high-temperature stress of vegetation ecosystem under high-emission scenarios. A threshold effect can be found between S _value change and precipitation, and the precipitation threshold is higher under the SSP5-8.5 scenario. The negative effect of temperature on CUE is attenuated by economic development and population control but this effect diminishes with rising CO _2 concentrations; in the future, developing clean energy and formulating population management policies can be used to enhance the carbon sink ability of the global ecosystem.

Purified red phosphorus (RP) can be used as an adsorbent. However, the adsorption mechanism and reuse ability of purified RP have not been reported. This study utilized X-ray diffraction, Fourier transform infrared spectroscopy and scanning electron microscopy techniques (a statistical physics model and the standard molar free energy of formation) to investigate the adsorption mechanism of methylene blue (MB) by purified RP. Purification did not change commercial RP structure according to X-ray diffraction. The results showed that the adsorption process only included physical adsorption according to Fourier transform infrared spectroscopy and UV–vis diffuse reflection absorption spectra. The specific areas of commercial RP and purified RP were 0.02 cm3/g and 5.27 cm3/g, respectively. Thus, purified RP has a higher adsorption capacity compared with commercial RP. A statistical physics model showed that, as the temperature increased from 288 to 308 K, the qe, Dm and qsat of purified RP for MB increased from 179.87, 0.824 and 0.824 to 303.26 mg/g, 1.497 mol/kg and 1.497 mol/kg, respectively. The fitted values of ΔrSmθ, ΔrHmθ and ΔrGmθ were 104.38 J·mol−1·K−1, −2.7 × 103 J·mol−1 and negative, respectively. Thus, according to adsorption energy, the adsorption of MB by purified RP was a spontaneous process, which was mainly driven by entropy increasing. Compared with neutral dye, the purified RP had higher adsorption ability for the cationic dye and anionic dye. As the purified RP dose increased from 30 to 150 mg, the adsorption capacity of purified RP increased. However, as the MB concentration and pH increased, the adsorption capacity of purified RP decreased. The purified RP had excellent reuse ability and high temperature desorption can be applied to obtain its reuse ability.

Abstract Background To investigate the stress changes between different bone cement forms and injection volumes in adjacent vertebrae after percutaneous kyphoplasty (PKP) by establishing a three-dimensional finite element model of osteoporosis. Methods A male healthy volunteer was selected. CT of scans L1 to L3 vertebrae were imported into Mimics 21.0 software.The vertebral model of osteoporosiswas established based on previous literature reference. The models were divided into three groups: unilateral, bilateral integration and bilateral separation groups, with each group injecting 2 ml, 4,ml and 6 ml of bone cement, respectively. In all models, a vertical compressive load of 500 N, anterior flexion/posterior extension, left/right bending, and left/right rotation were applied with a moment of 7.5 N/m, of which 85% was applied to the anterior mid-column and 15% to the posterior column. The stress changes between adjacent vertebrae under different conditions were calculated. Results After percutaneous kyphoplasty was applied to the L2 vertebral body, some differences can be found between the effects of different cement injection volumes and cement morphology on adjacent structures. There was no major difference between the groups when the bone cement injection volume was 2 ml. When the amount of bone cement injected was 4 ml, the bone cement morphology of the bilateral integration group (BIG) produced less stress between adjacent vertebral bodies. The minimum stress was 14.95 MPa in the L3 vertebral body in posterior extension. Whereas the stress levels on adjacent intervertebral structures, BIG shaped bone cement shows some superiority. In addition, the adjacent vertebrae and intervertebral structures are subjected to less stress during left and right rotation. Conclusions The present finite element study suggested that bilateral integration bone cement is a suitable form of cement injection, and when the injection volume is 4 ml, reduces stress on adjacent segments by approximately 15% while maintaining the stability of the injected vertebral body.

IntroductionMyostatin (MSTN) negatively regulates skeletal muscle development. However, its function in reproductive performance and visceral organs has not been thoroughly investigated. Previously, we prepared a MSTN and fibroblast growth factor 5 (FGF5) double-knockout sheep, which was a MSTN and FGF5 dual-gene biallelic homozygous (MF−/−) mutant.MethodsTo understand the role of MSTN and FGF5 in reproductive performance and visceral organs, this study evaluated the ejaculation amount, semen pH, sperm motility, sperm density, acrosome integrity, rate of teratosperm, and seminal plasma biochemical indicators in adult MF−/− rams. We also compared the overall morphology, head, head-neck junction, middle segment and the transection of middle segment of spermatozoa between wildtype (WT) and MF−/− rams.ResultsOur results showed that the seminal plasma biochemical indicators, sperm structure and all sperm indicators were normal, and the fertilization rate also has no significant difference between WT and MF−/− rams, indicating that the MF−/− mutation did not affect the reproductive performance of sheep. Additional analysis evaluated the histomorphology of the visceral organs, digestive system and reproductive system of MF+/− sheep, the F1 generation of MF−/−, at the age of 12 months. There was an increased spleen index, but no significant differences in the organ indexes of heart, liver, lung, kidney and stomach, and no obvious differences in the histomorphology of visceral organs, digestive system and reproductive system in MF+/− compared with WT sheep. No MF+/− sheep were observed to have any pathological features.DiscussionIn summary, the MSTN and FGF5 double-knockout did not affect reproductive performance, visceral organs and digestive system in sheep except for differences previously observed in muscle and fat. The current data provide a reference for further elucidating the application of MSTN and FGF5 double-knockout sheep.

Abstract As a major food crop and model organism, rice has been mostly studied with the largest number of functionally characterized genes among all crops. We previously built the funRiceGenes database including ~ 2800 functionally characterized rice genes and ~ 5000 members of different gene families. Since being published, the funRiceGenes database has been accessed by more than 54,400 users with over 540,000 pageviews. The funRiceGenes database has been continuously updated with newly cloned rice genes and newly published literature, based on the progress of rice functional genomics studies. Up to Nov 2021, ~ 4100 functionally characterized rice genes and ~ 6000 members of different gene families were collected in funRiceGenes, accounting for 22.3% of the 39,045 annotated protein-coding genes in the rice genome. Here, we summarized the update of the funRiceGenes database with new data and new features in the last 5 years.

Abstract A transmission phase‐shifted metasurface (PSM) array has been proposed for non‐diffractive beam deflection. A PSM element consists of two identical semicircular rings and an I‐shaped structure, which is symmetrically printed on both sides of the F4B substrate. According to the geometric optics, the phase distribution on the metasurface can be obtained. To achieve the desired phase distribution, the metasurface elements rotated at different angles are rearranged, since the phase of the transmitted wave is manipulated by rotating the metasurface element. Then, the standard horn is used to produce the sphere wave across the proposed metasurface array for the non‐diffractive beam deflection. Simulated (measured) results show that the inclined non‐diffractive beam can be generated at a tilted angle of 35° (34.9°) with a maximum non‐diffractive distance of 100 mm (97.5 mm) at 10 GHz. The proposed metasurface array has the advantages of non‐diffractive beam deflection, low sidelobes and miniaturisation.

The use of Diagnosis-Related Groups (DRG) is a prevalent payment system employed to control hospitalization costs and improve medical efficiency. China has developed an indigenized DRG payment system including Single Disease Payment (SDP), DRGs, and Big Data Diagnosis-Intervention Packet (DIP). In this study, we took cholecystitis as an example, drawing on both primary and secondary data to verify the effectiveness of China’s indigenized DRG system and to introduce the evolution of DRGs in China. Primary data were gathered from Qilu Hospital in 2019–2021. Secondary data were collected from published literature from 2004–2016. Only studies with both pre-SDP/DRG and post-SDP/DRG groups were included. Among the studies included, 92.9% (13/14) reported a significant reduction in hospitalization costs after the implementation of SDP while other studies identified length of stay (LOS) and age as the most significant influential factors in SDP. Furthermore, we elaborated the efficiency of DRGs using data from 2738 inpatients in Qilu hospital. Moreover, 60% (6/10) of the studies from the databases also showed the efficiency of DRGs in different regions. SDP is efficient in saving hospitalization costs, but its implementation is limited. DRGs have a broader scope of application, but their effectiveness remains to be validated. DIP is a brand new concept in China, and more data are needed to assess its efficiency.

Purpose – The anticipated benefits of connected and autonomous vehicles (CAVs) include safety and mobility enhancement. Small headways between successive vehicles, on one hand, can cause increased capacity and throughput and thereby improve overall mobility. On the other hand, small headways can cause vehicle occupant discomfort and unsafety. Therefore, in a CAV environment, it is important to determine appropriate headways that offer a good balance between mobility and user safety/comfort. Design/methodology/approach – In addressing this research question, this study carried out a pilot experiment using a driving simulator equipped with a Level-3 automated driving system, to measure the threshold headways. The Method of Constant Stimuli (MCS) procedure was modified to enable the estimation of two comfort thresholds. The participants (drivers) were placed in three categories (“Cautious,” “Neutral” and “Confident”) and 250 driving tests were carried out for each category. Probit analysis was then used to estimate the threshold headways that differentiate drivers' discomfort and their intention to re-engage the driving tasks. Findings – The results indicate that “Cautious” drivers tend to be more sensitive to the decrease in headways, and therefore exhibit greater propensity to deactivate the automated driving mode under a longer headway relative to other driver groups. Also, there seems to exist no driver discomfort when the CAV maintains headway up to 5%–9% shorter than the headways they typically adopt. Further reduction in headways tends to cause discomfort to drivers and trigger take over control maneuver. Research limitations/implications – In future studies, the number of observations could be increased further. Practical implications – The study findings can help guide specification of user-friendly headways specified in the algorithms used for CAV control, by vehicle manufacturers and technology companies. By measuring and learning from a human driver's perception, AV manufacturers can produce personalized AVs to suit the user's preferences regarding headway. Also, the identified headway thresholds could be applied by practitioners and researchers to update highway lane capacities and passenger-car-equivalents in the autonomous mobility era. Originality/value – The study represents a pioneering effort and preliminary pilot driving simulator experiment to assess the tradeoffs between comfortable headways versus mobility-enhancing headways in an automated driving environment.

This study empirically investigates the determinants and drivers of Hong Kong's inbound tourism using data from January 2019 to December 2020. Five of Hong Kong's major regional tourism source markets are selected: Japan, Malaysia, the Philippines, Singapore, and South Korea. Our empirical results suggest that the COVID-19 pandemic has had significant negative impacts on the tourism and aviation sectors in Hong Kong that are asymmetric in the source and destination countries. In addition, international travel control imposed by the Hong Kong government is often defined according to the pandemic situation in the tourism source countries, Hong Kong's inbound visitor volume is also significantly affected by the Hong Kong government's response to COVID-19 infection cases in the tourism origin markets. Our empirical results also suggest that the flying distance to Hong Kong and high tourism costs in Hong Kong reduced visitor arrival demand, whereas increased aviation services and tourism market potential contributed to tourism growth. These results suggest that the tourism industry and government agencies should cooperate to recover when the pandemic is under good control, so that Hong Kong will be regarded as a preferred and safe destination for travelers and visitors. Our study emphasizes the complementarity between pandemic control and tourism recovery. Pandemic control involves extra tests and quarantine requirements on passengers, more vigorous border control. These operational and associated financial requirements call for government support to the tourism and aviation sectors in the early stage of recovery to create a positive feedback loop.

To investigate the causes of the “sugar receding” in ‘Feizixiao’ litchi (Litchi chinensis Sonn.) pulp, the main sugar contents and sucrose metabolism enzyme activities were measured in pulp obtained in 2020 and 2021. Pulp RNA obtained in 2020 was extracted at 35, 63, and 69 days after anthesis (DAA) for transcriptome sequencing analysis. The differential expression of genes was verified by real-time PCR for both years. The results showed that after 63 DAA, the contents of soluble sugars and sucrose decreased, and the contents of fructose and glucose increased in both years. The dynamic changes in sucrose metabolism enzyme activities were similar in both years. After 63 DAA, except for acid invertase (AI) in 2021, the activities of other enzymes decreased significantly, and the net activity of sucrose metabolism enzymes showed a strong sucrose cleavage activity. There were 18061, 19575, and 985 differentially expressed genes in 35 d vs. 63 d, 35 d vs. 69 d, and 63 d vs. 69 d, respectively. Ninety-one sugar metabolism genes were screened out, including sucrose synthase (SS), sucrose phosphate synthase (SPS), AI, neutral invertase (NI), hexokinase (HK), glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), phosphofructokinase (PFK), and pyruvate kinase (PK) genes. In 63 d vs. 69 d, seventy-five percent of sucrose metabolism genes were downregulated, seventy-seven percent of genes in glycolysis (EMP) were upregulated and the PFK genes were significantly upregulated. There was a significant linear correlation between the expression of 15 genes detected by real-time PCR and the transcriptome sequencing results (r2020 = 0.9139, r2021 = 0.8912). These results suggest that the upregulated expression of PFK genes at maturity may enhance PFK activity and promote the degradation of soluble sugar in pulp through the EMP pathway, resulting in decreased soluble sugar and sucrose contents and “sugar receding” in pulp. Moreover, the downregulated expression of sucrose metabolism genes in pulp decreased the activities of these enzymes, but the net activity of these enzymes resulted in cleaved sucrose and replenished levels of reducing sugars, resulting in a stable reducing sugar content.

The transport loss generated by a high temperature superconducting coil (HTS) in an AC transmission process will affect the operational stability of the superconducting power equipment. However, under different self-field conditions, the influence of the oscillation waveform parameters and coil structure parameters on the transport loss still needs to be clarified. This article establishes a two-dimensional axisymmetric model with a single-pancake coil with a double strip winding as the research object. For four different oscillation waveforms—sine waves, square waves, triangular waves and sawtooth waves—the influence of four variables, namely, the transmission current amplitude, the coil radius, the number of turns of the coil, and the turn-to-turn spacing of the coil, on the transport loss of HTS coils is studied in detail. The results show that under the exact parameters of the coil, for the generated transport loss, the square wave is the largest, the sine wave is the second largest, and the sawtooth and triangular wave are the smallest. Under the same self-field condition, the transport loss can be reduced by increasing the coil radius, the number of turns of the coil, and the turn-to-turn spacing of the coil, in which if the number of turns of the single-pancake coil with a double strip winding is less than 14, the transport loss can be obviously reduced by increasing the number of turns of the coil. When the number of turns exceeds 14, it does little to reduce the transport loss. Research findings can confirm the investigation of the operational stability and design of HTS coils.

Abstract Background The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high efficiency. Despite the efforts have made on Artemisia annua transient expression, achieving high-throughput gene functional characterization basing on a fast and easy-manipulated transient transformation system in A. annua remains challenging. Results The first pair of true leaves of A. annua is an ideal candidate for Agrobacterium injection. EHA105 was the optimal strain that can be used for the development of the transient expression system. The supplementation of Triton X-100 at a concentration of 0.005% greatly improved the transient expression frequency. According to the histochemical β-Glucuronidase (GUS) staining assay, high transient expression level of the reporter gene (GUS) maintained at least a week. Dual-luciferase (Dual-LUC) transient assays showed that the activity of cauliflower mosaic virus 35S (CaMV35S) promoter and its derivates varied between A. annua and tobacco. In A. annua, the CaMV35S promoter had comparable activity with double CaMV35S promoter, while in tobacco, CaMV35S exhibited approximately 50% activity of double CaMV35S promoter. Otherwise, despite the CaMV35S promoter and double CaMV35S promoter from GoldenBraid Kit 2.0 displayed high activity strength in tobacco, they demonstrated a very low activity in transiently expressed A. annua. The activity of UBQ10 promoter and endogenous UBQb promoter was investigated as well. Additionally, using our transient expression system, the transactivation of AaGSW1 and AaORA on AaCYP71AV1 promoter was confirmed. Dual-LUC assays demonstrated that AaHD8 activated the expression of two glandular secreting trichomes-specific lipid transfer protein genes AaLTP1 and AaLTP2, indicating that AaLTP1 and AaLTP2 might serve as downstream components of AaHD8-involved glandular trichome initiation and cuticle formation, as well as artemisinin secretion in A. annua. Conclusions A simple, rapid, good-reproducibility, high-efficiency and low-cost transient transformation system in A. annua was developed. Our method offered a new way for gene functional characterization studies such as gene subcellular localization, promoter activity and transcription activation assays in A. annua, avoiding the aberrant phenotypes resulting from gene expression in a heterologous system.

Abstract Background To compare the clinical outcomes of C3 laminectomy and C3 laminoplasty at the C3 segment during French-door laminoplasty. Methods The Cochrane Library, PubMed, Embase, and Web of Science databases were searched from inception to November 10, 2020 for studies comparing the clinical outcomes of two types of French-door laminoplasty in the treatment of multilevel cervical spondylotic myelopathy (MCSM). Review Manager 5.3 was used to analyze the following outcomes: operative time, intraoperative blood loss, preoperative and postoperative Japanese Orthopaedic Association (JOA) scores, recovery rate, cervical curvature, cervical range of motion (ROM), incidence of axial symptoms (AS), and C2-3 bony fusion rate. Results A total of eight studies involving 776 patients were included; there were 424 patients in the C3 laminectomy group and 352 patients in the C3 laminoplasty group. The results of the meta-analysis showed that the C3 laminectomy group was superior to the C3 laminoplasty group in terms of operative time (P < 0.00001), cervical ROM (P = 0.04), and incidence of AS (P < 0.0001). However, no statistically significant differences between the two groups were noted regarding intraoperative bleeding (P = 0.44), preoperative JOA score (P = 0.57), postoperative JOA score (P = 0.09), recovery rate (P = 0.25), cervical curvature (P = 0.22), and C2-3 bony fusion rate (P = 0.06). Conclusion This meta-analysis demonstrated that both C3 laminoplasty and C3 laminectomy could effectively improve neurological function in patients with MCSM in French-door laminoplasty. However, C3 laminectomy can reduce the operative time, preserve cervical ROM, and reduce the incidence of postoperative AS. Trial registration PROSPERO registration number is CRD42021230798 . Date of registration: February 11, 2021.

Vegetation is an essential component of the earth’s surface system and its dynamics is a clear indicator of global climate change. However, the vegetation trends of most studies were based on time-unvarying methods, cannot accurately detect the long-term nonlinear characteristics of vegetation changes. Here, the ensemble empirical mode decomposition and the Breaks for Additive Seasonal and Trend algorithm were applied to reconstruct the the normalized difference vegetation index (NDVI) data and diagnose spatiotemporal evolution and abrupt changes of long-term vegetation trends in China during 1982–2018. Residual analysis was used to separate the influence of climate and human activities on NDVI variations, and the effect of specific human drivers on vegetation growth was obtained. The results suggest that based on the time-varying analysis, high vegetation browning was masked by overall vegetation greening. Vegetation growth in China experienced an abrupt change in the 1990s and 2000s, accounting for 50% and 33.6% of the whole China respectively. Of the area before the breakpoint, 45.4% showed a trend of vegetation decrease, which was concentrated mainly in east China, while 43% of the area after the breakpoint also showed vegetation degradation, mainly in northwest China. Climate was an important driving force for vegetation change in China. It played a positive role in south China, but had a negative effect in northwest China. The impact of human activities on vegetation growthchanged from an initial negative influence to a positive one. In terms of human activities, an inverted-U-shaped relation was detected between CO2 emissions and vegetation growth; that is, the fertilization effect of CO2 had a certain threshold. Once that threshold was exceeded, it would hinder vegetation growth. Population density had a slight constraint on vegetation growth, and the implementation of ecological restoration projects (e.g., the Grain for Green Program) can promote vegetation growth to a certain extent.

The dinoflagellate Akashiwo sanguinea is a harmful algal species and commonly observed in estuarine and coastal waters around the world. Harmful algal blooms (HABs) caused by this species lead to serious environmental impacts in the coastal waters of China since 1998 followed by huge economic losses. However, the full-length transcriptome information of A. sanguinea is still not fully explored, which hampers basic genetic and functional studies. Herein, single-molecule real-time (SMRT) sequencing technology was performed to characterize the full-length transcript in A. sanguinea. Totally, 83.03 Gb SMRT sequencing clean reads were generated, 983,960 circular consensus sequences (CCS) with average lengths of 3,061 bp were obtained, and 81.71% (804,016) of CCS were full-length non-chimeric reads (FLNC). Furthermore, 26,461 contigs were obtained after being corrected with Illumina library sequencing, with 20,037 (75.72%) successfully annotated in the five public databases. A total of 13,441 long non-coding RNA (lncRNA) transcripts, 3,137 alternative splicing (AS) events, 514 putative transcription factors (TFs) members from 23 TF families, and 4,397 simple sequence repeats (SSRs) were predicted, respectively. Our findings provided a sizable insights into gene sequence characteristics of A. sanguinea, which can be used as a reference sequence resource for A. sanguinea draft genome annotation, and will contribute to further molecular biology research on this harmful bloom algae.

Plant natural products (PNPs) are active substances indispensable to human health with a wide range of medical and commercial applications. However, excessive population growth, overexploitation of natural resources, and expensive total chemical synthesis have led to recurrent supply shortages. Despite the fact that the microbial production platform solved these challenges, the platform still has drawbacks such as environmental pollution, high costs, and non-green production. In this study, an efficient platform for the production of PNPs based on the transient expression system of Nicotiana benthamiana L. combined with synthetic biology strategies was developed. Subsequently, the feasibility of the platform was verified by a simple “test unit.” This platform was used to synthesize two high-value PNPs: genistein (5.51 nmol g–1 FW) and scutellarin (11.35 nmol g–1 FW). Importantly, this is the first report on the synthesis of scutellarin in heterologous plants. The platform presented here will possibly be adopted for the heterologous production of genistein and scutellarin in tobacco plants as a novel and sustainable production strategy.