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Abstract Community sanitation is a fundamental human right and need. Every year, as per the World Bank, total cost of providing sanitation services is estimated at around 114 billion USD per year. In India, Swachh Bharat Abhiyan (SBA), a public welfare scheme (PWS), is aimed at addressing community sanitation problems. Despite the successful implementation of SBA, local communities still practise open defaecation. To deduce the behavioural patterns governing communal toilet use, interviews were conducted with the local communities in the Kho Nagorian area of Jaipur, Rajasthan, India. This qualitative survey examined attitudes towards the construction of a toilet, awareness towards the SBA scheme, and the willingness to use excreta-based pit humus. The study then discusses the factors that increase the local community’s willingness to use these toilets. Results show that open defaecation is still prevalent in society. One way to foster the adoption of toilets is that the construction materials should mainly consist of local materials. As a recourse, places of worship could be used to influence people’s perception of hygiene. In addition, community toilets should be cleaned often as well. PWS should not be made accessible at no cost to prevent a sense of entitlement among the people. A small sum should be charged to increase social responsibility towards the PWS. Another way to curb open defaecation is to tap into the sense of entitlement by making effective use of social campaign programs. Further, cross-table analysis revealed that the locals were inclined to use a toilet if they have invested in it. Advertisements were found to be ineffective, and proposals were made to make them effective. These findings aid in understanding public perceptions and can guide the development of public policies. The findings also assist in making tax distribution decisions that reflect public concerns, attitudes, and values.

India is a multilingual country. Hindi is the national language of India and Devanãgri script is used to write Hindi language. Various documents with legal value are made in Devanãgri script, which may be questioned for their authenticity and authorship. Sufficient research has been done and reported about different scripts. However, researches based on Devanãgri script are limited. This study focuses on finding the significant class characteristics of writers from three different states of India, namely, Bihar, Madhya Pradesh and Punjab. The data was also statistically analysed using Pearson’s Chi-Square test for its significance. Handwriting samples from 300 subjects were collected from three different states of India to analyze various class characteristics in Devanãgri script. The samples were examined qualitatively and statistically. Various general characteristics were selected for the analysis and characteristic features were tabulated after qualitative examination. Statistical analysis showed that the data was statistically significant. The general characteristics selected for the analysis and comparison of the handwriting samples in Devanãgri script were found to be significant. The impact of regional scripts on the Devanãgri script should be performed, as the influence of regional language could be seen in the samples collected from Punjab.

On the basis of the type of ink, pen inks are classified as ballpoint pens or non-ballpoint pens. Ballpoint pen inks are oil-based inks (ballpoint pens). While non-ballpoint pen inks are water-based inks (gel pens, fountain pens, pilot pens, fiber tip pens, etc.). Different approaches related to the determination of the age of ink were proposed in the literature, including ballpoint pen inks, non-ballpoint pen inks and stamp pad inks. This study provides a comprehensive appreciation of analytical research studies that were published from the year 2000 to year 2020 on the determination of the age of inks. Owing To contemporary technological advancements, the process has been developed in many folds. However, its reliability is questioned due to the variability in methods and results. Research works related to the aging of ink have been compiled to assist the researchers and the forensic document examiners and to provide an overall insight regarding the works done so far. In an attempt to unravel this issue of questioned document examination, a critical and systematic review of the literature is proposed to encourage the usage of various tools and techniques necessary to establish the age of ink in different case works.

Ets variant 2 (Etv2), a member of the Ets factor family, has an essential role in the formation of endothelial and hematopoietic cell lineages during embryonic development. The functional role of ETS transcription factors is, in part, dependent on the interacting proteins. There are relatively few studies exploring the coordinated interplay between ETV2 and its interacting proteins that regulate mesodermal lineage determination. In order to identify novel ETV2 interacting partners, a yeast two-hybrid analysis was performed and the C2H2 zinc finger transcription factor VEZF1 (vascular endothelial zinc finger 1) was identified as a binding factor, which was specifically expressed within the endothelium during vascular development. To confirm this interaction, co-immunoprecipitation and GST pull down assays demonstrated the direct interaction between ETV2 and VEZF1. During embryoid body differentiation, Etv2 achieved its peak expression at day 3.0 followed by rapid downregulation, on the other hand Vezf1 expression increased through day 6 of EB differentiation. We have previously shown that ETV2 potently activated Flt1 gene transcription. Using a Flt1 promoter-luciferase reporter assay, we demonstrated that VEZF1 co-activated the Flt1 promoter. Electrophoretic mobility shift assay and Chromatin immunoprecipitation established VEZF1 binding to the Flt1 promoter. Vezf1 knockout embryonic stem cells had downregulation of hematoendothelial marker genes when undergoing embryoid body mediated mesodermal differentiation whereas overexpression of VEZF1 induced the expression of hematoendothelial genes during differentiation. These current studies provide insight into the co-regulation of the hemato-endothelial lineage development via a co-operative interaction between ETV2 and VEZF1.

The reaction of phenols with 7aled to the synthesis of aurones, while the reaction of phenols with 7bled to the synthesis of flavones. [ABSTRACT FROM AUTHOR]