25 results on '"Voellenkle, Christine"'
Search Results
2. Coding and Non-Coding Transcriptomic Landscape of Aortic Complications in Marfan Syndrome.
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Udugampolage, Nathasha Samali, Frolova, Svetlana, Taurino, Jacopo, Pini, Alessandro, Martelli, Fabio, and Voellenkle, Christine
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MARFAN syndrome ,THORACIC aneurysms ,TRANSCRIPTOMES ,CONGENITAL disorders ,AORTA - Abstract
Marfan syndrome (MFS) is a rare congenital disorder of the connective tissue, leading to thoracic aortic aneurysms (TAA) and dissection, among other complications. Currently, the most efficient strategy to prevent life-threatening dissection is preventive surgery. Periodic imaging applying complex techniques is required to monitor TAA progression and to guide the timing of surgical intervention. Thus, there is an acute demand for non-invasive biomarkers for diagnosis and prognosis, as well as for innovative therapeutic targets of MFS. Unraveling the intricate pathomolecular mechanisms underlying the syndrome is vital to address these needs. High-throughput platforms are particularly well-suited for this purpose, as they enable the integration of different datasets, such as transcriptomic and epigenetic profiles. In this narrative review, we summarize relevant studies investigating changes in both the coding and non-coding transcriptome and epigenome in MFS-induced TAA. The collective findings highlight the implicated pathways, such as TGF-β signaling, extracellular matrix structure, inflammation, and mitochondrial dysfunction. Potential candidates as biomarkers, such as miR-200c, as well as therapeutic targets emerged, like Tfam, associated with mitochondrial respiration, or miR-632, stimulating endothelial-to-mesenchymal transition. While these discoveries are promising, rigorous and extensive validation in large patient cohorts is indispensable to confirm their clinical relevance and therapeutic potential. [ABSTRACT FROM AUTHOR]
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- 2024
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- View/download PDF
3. Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia
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Zaccagnini Germana, Greco Simona, Longo Marialucia, Maimone Biagina, Voellenkle Christine, Fuschi Paola, Carrara Matteo, Creo Pasquale, Maselli Davide, Tirone Mario, Mazzone Massimiliano, Gaetano Carlo, Spinetti Gaia, and Martelli Fabio
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Cytology ,QH573-671 - Abstract
Abstract Hypoxia-induced miR-210 is a crucial component of the tissue response to ischemia, stimulating angiogenesis and improving tissue regeneration. Previous analysis of miR-210 impact on the transcriptome in a mouse model of hindlimb ischemia showed that miR-210 regulated not only vascular regeneration functions, but also inflammation. To investigate this event, doxycycline-inducible miR-210 transgenic mice (Tg-210) and anti-miR-210 LNA-oligonucleotides were used. It was found that global miR-210 expression decreased inflammatory cells density and macrophages accumulation in the ischemic tissue. To dissect the underpinning cell mechanisms, Tg-210 mice were used in bone marrow (BM) transplantation experiments and chimeric mice underwent hindlimb ischemia. MiR-210 overexpression in the ischemic tissue was sufficient to increase capillary density and tissue repair, and to reduce inflammation in the presence of Wt-BM infiltrating cells. Conversely, when Tg-210-BM cells migrated in a Wt ischemic tissue, dysfunctional angiogenesis, inflammation, and impaired tissue repair, accompanied by fibrosis were observed. The fibrotic regions were positive for α-SMA, Vimentin, and Collagen V fibrotic markers and for phospho-Smad3, highlighting the activation of TGF-β1 pathway. Identification of Tg-210 cells by in situ hybridization showed that BM-derived cells contributed directly to fibrotic areas, where macrophages co-expressing fibrotic markers were observed. Cell cultures of Tg-210 BM-derived macrophages exhibited a pro-fibrotic phenotype and were enriched with myofibroblast-like cells, which expressed canonical fibrosis markers. Interestingly, inhibitors of TGF-β type-1-receptor completely abrogated this pro-fibrotic phenotype. In conclusion, a context-dependent regulation by miR-210 of the inflammatory response was identified. miR-210 expression in infiltrating macrophages is associated to improved angiogenesis and tissue repair when the ischemic recipient tissue also expresses high levels of miR-210. Conversely, when infiltrating an ischemic tissue with mismatched miR-210 levels, macrophages expressing high miR-210 levels display a pro-fibrotic phenotype, leading to impaired tissue repair, fibrosis, and dysfunctional angiogenesis.
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- 2021
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4. Dysregulation of microRNA expression in diabetic skin
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Baldini, Enke, Testa, Erika, Voellenkle, Christine, De Domenico, Emanuela, Cianfarani, Francesca, Martelli, Fabio, Ulisse, Salvatore, and Odorisio, Teresa
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- 2020
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5. Correction: Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia
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Zaccagnini, Germana, Greco, Simona, Longo, Marialucia, Maimone, Biagina, Voellenkle, Christine, Fuschi, Paola, Carrara, Matteo, Creo, Pasquale, Maselli, Davide, Tirone, Mario, Mazzone, Massimiliano, Gaetano, Carlo, Spinetti, Gaia, and Martelli, Fabio
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- 2021
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6. circRNA-miRNA-mRNA Deregulated Network in Ischemic Heart Failure Patients.
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Madè, Alisia, Bibi, Alessia, Garcia-Manteiga, Jose Manuel, Tascini, Anna Sofia, Piella, Santiago Nicolas, Tikhomirov, Roman, Voellenkle, Christine, Gaetano, Carlo, Leszek, Przemyslaw, Castelvecchio, Serenella, Menicanti, Lorenzo, Martelli, Fabio, and Greco, Simona
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CIRCULAR RNA ,HEART failure patients ,NON-coding RNA ,ENDOTHELIAL cells ,CELL cycle ,MESSENGER RNA - Abstract
Noncoding RNAs (ncRNAs), which include circular RNAs (circRNAs) and microRNAs (miRNAs), regulate the development of cardiovascular diseases (CVD). Notably, circRNAs can interact with miRNAs, influencing their specific mRNA targets' levels and shaping a competing endogenous RNAs (ceRNA) network. However, these interactions and their respective functions remain largely unexplored in ischemic heart failure (IHF). This study is aimed at identifying circRNA-centered ceRNA networks in non-end-stage IHF. Approximately 662 circRNA-miRNA-mRNA interactions were identified in the heart by combining state-of-the-art bioinformatics tools with experimental data. Importantly, KEGG terms of the enriched mRNA indicated CVD-related signaling pathways. A specific network centered on circBPTF was validated experimentally. The levels of let-7a-5p, miR-18a-3p, miR-146b-5p, and miR-196b-5p were enriched in circBPTF pull-down experiments, and circBPTF silencing inhibited the expression of HDAC9 and LRRC17, which are targets of miR-196b-5p. Furthermore, as suggested by the enriched pathway terms of the circBPTF ceRNA network, circBPTF inhibition elicited endothelial cell cycle arrest. circBPTF expression increased in endothelial cells exposed to hypoxia, and its upregulation was confirmed in cardiac samples of 36 end-stage IHF patients compared to healthy controls. In conclusion, circRNAs act as miRNA sponges, regulating the functions of multiple mRNA targets, thus providing a novel vision of HF pathogenesis and laying the theoretical foundation for further experimental studies. [ABSTRACT FROM AUTHOR]
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- 2023
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7. Increased BACE1-AS long noncoding RNA and β-amyloid levels in heart failure
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Greco, Simona, Zaccagnini, Germana, Fuschi, Paola, Voellenkle, Christine, Carrara, Matteo, Sadeghi, Iman, Bearzi, Claudia, Maimone, Biagina, Castelvecchio, Serenella, Stellos, Konstantinos, Gaetano, Carlo, Menicanti, Lorenzo, and Martelli, Fabio
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- 2017
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8. CircANKRD12 Is Induced in Endothelial Cell Response to Oxidative Stress.
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Voellenkle, Christine, Fuschi, Paola, Mutoli, Martina, Carrara, Matteo, Righini, Paolo, Nano, Giovanni, Gaetano, Carlo, and Martelli, Fabio
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CIRCULAR RNA , *ENDOTHELIAL cells , *OXIDATIVE stress , *CELLULAR signal transduction , *SKELETAL muscle , *TRANSCRIPTOMES , *CARDIOVASCULAR diseases - Abstract
Redox imbalance of the endothelial cells (ECs) plays a causative role in a variety of cardiovascular diseases. In order to better understand the molecular mechanisms of the endothelial response to oxidative stress, the involvement of circular RNAs (circRNAs) was investigated. CircRNAs are RNA species generated by a "back-splicing" event, which is the covalent linking of the 3′- and 5′-ends of exons. Bioinformatics analysis of the transcriptomic landscape of human ECs exposed to H2O2 allowed us to identify a subset of highly expressed circRNAs compared to their linear RNA counterparts, suggesting a potential biological relevance. Specifically, circular Ankyrin Repeat Domain 12 (circANKRD12), derived from the junction of exon 2 and exon 8 of the ANKRD12 gene (hsa_circ_0000826), was significantly induced in H2O2-treated ECs. Conversely, the linear RNA isoform of ANKRD12 was not modulated. An increased circular-to-linear ratio of ANKRD12 was also observed in cultured ECs exposed to hypoxia and in skeletal muscle biopsies of patients affected by critical limb ischemia (CLI), two conditions associated with redox imbalance and oxidative stress. The functional relevance of circANKRD12 was shown by the inhibition of EC formation of capillary-like structures upon silencing of the circular but not of the linear isoform of ANKRD12. Bioinformatics analysis of the circANKRD12–miRNA–mRNA regulatory network in H2O2-treated ECs identified the enrichment of the p53 and Foxo signaling pathways, both crucial in the cellular response to redox imbalance. In keeping with the antiproliferative action of the p53 pathway, circANKRD12 silencing inhibited EC proliferation. In conclusion, this study indicates circANKRD12 as an important player in ECs exposed to oxidative stress. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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9. miR-210 hypoxamiR in Angiogenesis and Diabetes.
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Zaccagnini, Germana, Greco, Simona, Voellenkle, Christine, Gaetano, Carlo, and Martelli, Fabio
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- 2022
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10. Long noncoding RNA dysregulation in ischemic heart failure.
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Greco, Simona, Zaccagnini, Germana, Perfetti, Alessandra, Fuschi, Paola, Valaperta, Rea, Voellenkle, Christine, Castelvecchio, Serenella, Gaetano, Carlo, Finato, Nicoletta, Beltrami, Antonio Paolo, Menicanti, Lorenzo, and Martelli, Fabio
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HEART failure ,NON-coding RNA ,RNA physiology ,LEFT heart ventricle diseases ,HEART failure patients ,GENE ontology ,GENETICS ,CORONARY heart disease complications ,RNA metabolism ,ANIMAL experimentation ,BIOLOGICAL models ,CHRONIC diseases ,CORONARY disease ,GENES ,CARDIAC hypertrophy ,MICE ,RESEARCH evaluation ,RESEARCH funding ,RNA ,GENE expression profiling ,DISEASE complications - Abstract
Background: Long noncoding RNAs (lncRNAs) are non-protein coding transcripts regulating a variety of physiological and pathological functions. However, their implication in heart failure is still largely unknown. The aim of this study is to identify and characterize lncRNAs deregulated in patients affected by ischemic heart failure.Methods: LncRNAs were profiled and validated in left ventricle biopsies of 18 patients affected by non end-stage dilated ischemic cardiomyopathy and 17 matched controls. Further validations were performed in left ventricle samples derived from explanted hearts of end-stage heart failure patients and in a mouse model of cardiac hypertrophy, obtained by transverse aortic constriction. Peripheral blood mononuclear cells of heart failure patients were also analyzed. LncRNA distribution in the heart was assessed by in situ hybridization. Function of the deregulated lncRNA was explored analyzing the expression of the neighbor mRNAs and by gene ontology analysis of the correlating coding transcripts.Results: Fourteen lncRNAs were significantly modulated in non end-stage heart failure patients, identifying a heart failure lncRNA signature. Nine of these lncRNAs (CDKN2B-AS1/ANRIL, EGOT, H19, HOTAIR, LOC285194/TUSC7, RMRP, RNY5, SOX2-OT and SRA1) were also confirmed in end-stage failing hearts. Intriguingly, among the conserved lncRNAs, h19, rmrp and hotair were also induced in a mouse model of heart hypertrophy. CDKN2B-AS1/ANRIL, HOTAIR and LOC285194/TUSC7 showed similar modulation in peripheral blood mononuclear cells and heart tissue, suggesting a potential role as disease biomarkers. Interestingly, RMRP displayed a ubiquitous nuclear distribution, while H19 RNA was more abundant in blood vessels and was both cytoplasmic and nuclear. Gene ontology analysis of the mRNAs displaying a significant correlation in expression with heart failure lncRNAs identified numerous pathways and functions involved in heart failure progression.Conclusions: These data strongly suggest lncRNA implication in the molecular mechanisms underpinning HF. [ABSTRACT FROM AUTHOR]- Published
- 2016
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11. ROD1 Is a Seedless Target Gene of Hypoxia-Induced miR-210.
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Fasanaro, Pasquale, Romani, Sveva, Voellenkle, Christine, Maimone, Biagina, Capogrossi, Maurizio C., Martelli, Fabio, and Gangjian Qin
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HYPOXEMIA ,METAZOA ,MICRORNA ,PROTEIN research ,CELL death ,ALGORITHMS - Abstract
Most metazoan microRNA (miRNA) target sites have perfect pairing to the "seed" sequence, a highly conserved region centering on miRNA nucleotides 2-7. Thus, complementarity to this region is a necessary requirement for target prediction algorithms. However, also non-canonical miRNA binding can confer target regulation. Here, we identified a seedless target of miR-210, a master miRNA of the hypoxic response. We analyzed 20 genes that were inversely correlated to miR-210 expression and did not display any complementarity with miR-210 seed sequence. We validated ROD1 (Regulator of Differentiation 1, also named PTBP3, Polypyrimidine Tract Binding protein 3) as a miR-210 seedless transcript enriched in miR-210-containing RNA-induced silencing complexes. ROD1 was not indirectly targeted by a miR-210-induced miRNA. Conversely, we identified a "centered" miR-210 binding site in ROD1 involving 10 consecutive bases in the central portion of miR-210. Reporter assays showed that miR-210 inhibited ROD1 by the direct binding to this sequence, demonstrating that ROD1 is a bona fide seedless target of miR-210. As expected, both ROD1 mRNA and protein were down-modulated upon hypoxia in a miR-210 dependent manner. ROD1 targeting by miR-210 was biologically significant: the rescue of ROD1 inhibition significantly increased hypoxia-induced cell death. These data highlight the importance of ROD1 regulation by miR-210 for cell homeostasis. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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12. ChIP-on-Chip Analysis of In Vivo Mutant p53 Binding To Selected Gene Promoters.
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Dell'Orso, Stefania, Fontemaggi, Giulia, Stambolsky, Perry, Goeman, Frauke, Voellenkle, Christine, Levrero, Massimo, Strano, Sabrina, Rotter, Varda, Oren, Moshe, and Blandino, Giovanni
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- 2011
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13. Hypoxia-Induced miR-210 Is Necessary for Vascular Regeneration upon Acute Limb Ischemia.
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Zaccagnini, Germana, Maimone, Biagina, Fuschi, Paola, Longo, Marialucia, Da Silva, Daniel, Carrara, Matteo, Voellenkle, Christine, Perani, Laura, Esposito, Antonio, Gaetano, Carlo, and Martelli, Fabio
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VENTRICULAR remodeling ,ISCHEMIA ,PERIPHERAL vascular diseases ,MYOCARDIAL infarction ,LIFE expectancy ,TRANSGENIC mice - Abstract
Critical limb ischemia is the most serious form of peripheral artery disease, characterized by severe functional consequences, difficult clinical management and reduced life expectancy. The goal of this study was to investigate the miR-210 role in the neo-angiogenic response after acute limb ischemia. Complementary approaches were used in a mouse model of hindlimb ischemia: miR-210 loss-of-function was obtained by administration of LNA-oligonucleotides anti-miR-210; for miR-210 gain-of-function, a doxycycline-inducible miR-210 transgenic mouse was used. We tested miR-210 ability to stimulate vascular regeneration following ischemia. We found that miR-210 was necessary and sufficient to stimulate blood perfusion recovery, as well as arteriolar and capillary density increase, in the ischemic muscle. To clarify the molecular events underpinning miR-210 pro-angiogenic action, the transcriptomic changes in ischemic muscles upon miR-210 blocking were analyzed. We found that miR-210 impacted the transcriptome significantly, regulating pathways and functions linked to vascular regeneration. In agreement with a pro-angiogenic role, miR-210 also improved cardiac function and left ventricular remodeling after myocardial infarction. Moreover, miR-210 blocking decreased capillary density in a Matrigel plug assay, indicating that miR-210 is necessary for angiogenesis independently of ischemia. Collectively, these data indicate that miR-210 plays a pivotal role in promoting vascular regeneration. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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14. Dysregulation of Circular RNAs in Myotonic Dystrophy Type 1.
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Voellenkle, Christine, Perfetti, Alessandra, Carrara, Matteo, Fuschi, Paola, Renna, Laura Valentina, Longo, Marialucia, Sain, Simona Baghai, Cardani, Rosanna, Valaperta, Rea, Silvestri, Gabriella, Legnini, Ivano, Bozzoni, Irene, Furling, Denis, Gaetano, Carlo, Falcone, Germana, Meola, Giovanni, and Martelli, Fabio
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CIRCULAR RNA , *MYOTONIA atrophica , *MICRORNA , *RNA sequencing , *SKELETAL muscle - Abstract
Circular RNAs (circRNAs) constitute a recently re-discovered class of non-coding RNAs functioning as sponges for miRNAs and proteins, affecting RNA splicing and regulating transcription. CircRNAs are generated by "back-splicing", which is the linking covalently of 3′- and 5′-ends of exons. Thus, circRNA levels might be deregulated in conditions associated with altered RNA-splicing. Significantly, growing evidence indicates their role in human diseases. Specifically, myotonic dystrophy type 1 (DM1) is a multisystemic disorder caused by expanded CTG repeats in the DMPK gene which results in abnormal mRNA-splicing. In this investigation, circRNAs expressed in DM1 skeletal muscles were identified by analyzing RNA-sequencing data-sets followed by qPCR validation. In muscle biopsies, out of nine tested, four transcripts showed an increased circular fraction: CDYL, HIPK3, RTN4_03, and ZNF609. Their circular fraction values correlated with skeletal muscle strength and with splicing biomarkers of disease severity, and displayed higher values in more severely affected patients. Moreover, Receiver-Operating-Characteristics curves of these four circRNAs discriminated DM1 patients from controls. The identified circRNAs were also detectable in peripheral-blood-mononuclear-cells (PBMCs) and the plasma of DM1 patients, but they were not regulated significantly. Finally, increased circular fractions of RTN4_03 and ZNF609 were also observed in differentiated myogenic cell lines derived from DM1 patients. In conclusion, this pilot study identified circRNA dysregulation in DM1 patients. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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15. p75NTR-dependent activation of NF-κB regulates microRNA-503 transcription and pericyte-endothelial crosstalk in diabetes after limb ischaemia.
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Caporali, Andrea, Meloni, Marco, Nailor, Audrey, Mitić, Tijana, Shantikumar, Saran, Riu, Federica, Sala-Newby, Graciela B., Rose, Lorraine, Besnier, Marie, Katare, Rajesh, Voellenkle, Christine, Verkade, Paul, Martelli, Fabio, Madeddu, Paolo, and Emanueli, Costanza
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- 2015
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16. Transcriptional Profiling of Hmgb1-Induced Myocardial Repair Identifies a Key Role for Notch Signaling.
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Limana, Federica, Esposito, Grazia, Fasanaro, Pasquale, Foglio, Eleonora, Arcelli, Diego, Voellenkle, Christine, Di Carlo, Anna, Avitabile, Daniele, Martelli, Fabio, Antonio Russo, Matteo, Pompilio, Giulio, Germani, Antonia, and C Capogrossi, Maurizio
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MYOCARDIAL infarction , *ANIMAL models in research , *GENE expression , *GENETIC regulation , *REGENERATION (Biology) - Abstract
Exogenous high-mobility group box 1 protein (HMGB1) administration to the mouse heart, during acute myocardial infarction (MI), results in cardiac regeneration via resident c-kit+ cell (CPC) activation. Aim of the present study was to identify the molecular pathways involved in HMGB1-induced heart repair. Gene expression profiling was performed to identify differentially expressed genes in the infarcted and bordering regions of untreated and HMGB1-treated mouse hearts, 3 days after MI. Functional categorization of the transcripts, accomplished using Ingenuity Pathway Analysis software (IPA), revealed that genes involved in tissue regeneration, that is, cardiogenesis, vasculogenesis and angiogenesis, were present both in the infarcted area and in the peri-infarct zone; HMGB1 treatment further increased the expression of these genes. IPA revealed the involvement of Notch signaling pathways in HMGB1-treated hearts. Importantly, HMGB1 determined a 35 and 58% increase in cardiomyocytes and CPCs expressing Notch intracellular cytoplasmic domain, respectively. Further, Notch inhibition by systemic treatment with the γ-secretase inhibitor DAPT, which blocked the proteolytic activation of Notch receptors, reduced the number of CPCs, their proliferative fraction, and cardiomyogenic differentiation in HMGB1-treated infarcted hearts. The present study gives insight into the molecular processes involved in HMGB1-mediated cardiac regeneration and indicates Notch signaling as a key player.Molecular Therapy (2013); 21 10, 1841-1851. doi:10.1038/mt.2013.137 [ABSTRACT FROM AUTHOR]
- Published
- 2013
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17. Time-controlled and muscle-specific CRISPR/Cas9-mediated deletion of CTG-repeat expansion in the DMPK gene.
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Cardinali B, Provenzano C, Izzo M, Voellenkle C, Battistini J, Strimpakos G, Golini E, Mandillo S, Scavizzi F, Raspa M, Perfetti A, Baci D, Lazarevic D, Garcia-Manteiga JM, Gourdon G, Martelli F, and Falcone G
- Abstract
CRISPR/Cas9-mediated therapeutic gene editing is a promising technology for durable treatment of incurable monogenic diseases such as myotonic dystrophies. Gene-editing approaches have been recently applied to in vitro and in vivo models of myotonic dystrophy type 1 (DM1) to delete the pathogenic CTG-repeat expansion located in the 3' untranslated region of the DMPK gene. In DM1-patient-derived cells removal of the expanded repeats induced beneficial effects on major hallmarks of the disease with reduction in DMPK transcript-containing ribonuclear foci and reversal of aberrant splicing patterns. Here, we set out to excise the triplet expansion in a time-restricted and cell-specific fashion to minimize the potential occurrence of unintended events in off-target genomic loci and select for the target cell type. To this aim, we employed either a ubiquitous promoter-driven or a muscle-specific promoter-driven Cas9 nuclease and tetracycline repressor-based guide RNAs. A dual-vector approach was used to deliver the CRISPR/Cas9 components into DM1 patient-derived cells and in skeletal muscle of a DM1 mouse model. In this way, we obtained efficient and inducible gene editing both in proliferating cells and differentiated post-mitotic myocytes in vitro as well as in skeletal muscle tissue in vivo ., Competing Interests: The authors declare no competing interests., (© 2021 The Authors.)
- Published
- 2021
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18. Hypoxia-Induced miR-210 Is Necessary for Vascular Regeneration upon Acute Limb Ischemia.
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Zaccagnini G, Maimone B, Fuschi P, Longo M, Da Silva D, Carrara M, Voellenkle C, Perani L, Esposito A, Gaetano C, and Martelli F
- Subjects
- Animals, Disease Models, Animal, Female, Ischemia genetics, Male, Mice, Mice, Inbred C57BL, MicroRNAs genetics, Myocardial Infarction genetics, Myocardial Infarction metabolism, Neovascularization, Physiologic genetics, Hindlimb pathology, Ischemia metabolism, MicroRNAs metabolism, Muscle, Skeletal metabolism, Neovascularization, Physiologic physiology
- Abstract
Critical limb ischemia is the most serious form of peripheral artery disease, characterized by severe functional consequences, difficult clinical management and reduced life expectancy. The goal of this study was to investigate the miR-210 role in the neo-angiogenic response after acute limb ischemia. Complementary approaches were used in a mouse model of hindlimb ischemia: miR-210 loss-of-function was obtained by administration of LNA-oligonucleotides anti-miR-210; for miR-210 gain-of-function, a doxycycline-inducible miR-210 transgenic mouse was used. We tested miR-210 ability to stimulate vascular regeneration following ischemia. We found that miR-210 was necessary and sufficient to stimulate blood perfusion recovery, as well as arteriolar and capillary density increase, in the ischemic muscle. To clarify the molecular events underpinning miR-210 pro-angiogenic action, the transcriptomic changes in ischemic muscles upon miR-210 blocking were analyzed. We found that miR-210 impacted the transcriptome significantly, regulating pathways and functions linked to vascular regeneration. In agreement with a pro-angiogenic role, miR-210 also improved cardiac function and left ventricular remodeling after myocardial infarction. Moreover, miR-210 blocking decreased capillary density in a Matrigel plug assay, indicating that miR-210 is necessary for angiogenesis independently of ischemia. Collectively, these data indicate that miR-210 plays a pivotal role in promoting vascular regeneration.
- Published
- 2019
- Full Text
- View/download PDF
19. Central role of the p53 pathway in the noncoding-RNA response to oxidative stress.
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Fuschi P, Carrara M, Voellenkle C, Garcia-Manteiga JM, Righini P, Maimone B, Sangalli E, Villa F, Specchia C, Picozza M, Nano G, Gaetano C, Spinetti G, Puca AA, Magenta A, and Martelli F
- Subjects
- Aged, Aged, 80 and over, Cell Line, Female, Human Umbilical Vein Endothelial Cells drug effects, Human Umbilical Vein Endothelial Cells metabolism, Humans, Hydrogen Peroxide pharmacology, Male, Oxidants pharmacology, Oxidation-Reduction, Transcriptome, Gene Expression Regulation physiology, Oxidative Stress physiology, RNA, Untranslated biosynthesis, Tumor Suppressor Protein p53 metabolism
- Abstract
Oxidative stress plays a fundamental role in many conditions. Specifically, redox imbalance inhibits endothelial cell (EC) growth, inducing cell death and senescence. We used global transcriptome profiling to investigate the involvement of noncoding-RNAs in these phenotypes. By RNA-sequencing, transcriptome changes were analyzed in human ECs exposed to H
2 O2 , highlighting a pivotal role of p53-signaling. Bioinformatic analysis and validation in p53-silenced ECs, identified several p53-targets among both mRNAs and long noncoding-RNAs (lncRNAs), including MALAT1 and NEAT1. Among microRNAs (miRNAs), miR-192-5p was the most induced by H2 O2 treatment, in a p53-dependent manner. Down-modulated mRNA-targets of miR-192-5p were involved in cell cycle, DNA repair and stress response. Accordingly, miR-192-5p overexpression significantly decreased EC proliferation, inducing cell death. A central role of the p53-pathway was also confirmed by the analysis of differential exon usage: Upon H2 O2 treatment, the expression of p53-dependent 5'-isoforms of MDM2 and PVT1 increased selectively. The transcriptomic alterations identified in H2 O2 -treated ECs were also observed in other physiological and pathological conditions where redox control plays a fundamental role, such as ECs undergoing replicative senescence, skeletal muscles of critical limb-ischemia patients and the peripheral-blood mononuclear cells of long-living individuals. Collectively, these findings indicate a prominent role of noncoding-RNAs in oxidative stress response.- Published
- 2017
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20. The expression of the BPIFB4 and CXCR4 associates with sustained health in long-living individuals from Cilento-Italy.
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Spinetti G, Sangalli E, Specchia C, Villa F, Spinelli C, Pipolo R, Carrizzo A, Greco S, Voellenkle C, Vecchione C, Madeddu P, Martelli F, and Puca AA
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- Adrenomedullin genetics, Aged, 80 and over, Cell Movement physiology, Female, Glucose Transporter Type 1 genetics, Health Status, Humans, Intercellular Signaling Peptides and Proteins, Italy, Leukocytes, Mononuclear cytology, Leukocytes, Mononuclear metabolism, Male, Vascular Endothelial Growth Factor A genetics, Aging genetics, Longevity genetics, Phosphoproteins genetics, Receptors, CXCR4 genetics
- Abstract
The study of the health status in long-living individuals (LLIs) may help identifying health-span and life-span determinants. BPI-Fold-Containing-Family-B-Member-4 (BPIFB4) protein is higher in healthy vs. non-healthy (frail) LLIs serum and its longevity-associated variant forced expression improves cardiovascular outcomes in ischemia mice models. Thus, we tested the association of BPIFB4 and ischemia-responding HIF-1α pathway components (i.e. CXCR4 , AK3, ALDO-C, ADM , VEGF-A , GLUT-1 and miR-210) with human life-span and health-span by analyzing mRNA expression in circulating mononuclear cells (MNCs) of LLIs (N=14 healthy; N=31 frail) and young controls (N=63). ALDO-C, ADM , VEGF-A and GLUT-1 significantly decreased and miR-210 increased in LLIs vs., Controls: Only VEGF-A and GLUT-1 showed further significant reduction in healthy-LLIs vs. frail-LLIs comparison. While BPIFB4 and CXCR4 were similar between LLIs and controls, BPIFB4 was significantly higher and CXCR4 lower in healthy- versus frail-LLIs. On a new set of LLIs (N=7 healthy and N=5 non-healthy) we assessed a potentially correlated function with low CXCR4 expression. Healthy donors' MNCs showed efficient migration ability toward CXCR4 ligand SDF-1α/CXCL12 and high percentage of migrated CXCR4
pos cells which inversely correlated with CXCR4 RNA expression. In conclusion, BPIFB4 and CXCR4 expression classify LLIs health status that correlates with maintained MNCs migration.- Published
- 2017
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21. microRNAs in ischaemic cardiovascular diseases.
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Greco S, Zaccagnini G, Voellenkle C, and Martelli F
- Abstract
microRNAs (miRNAs) are non-coding RNA molecules that modulate the stability and/or the translational efficiency of specific messenger RNAs. They have been shown to play a regulatory role in most biological processes and their expression is disrupted in many cardiovascular diseases. This review describes studies performed at Policlinico San Donato-IRCCS in cell cultures, animal models, and patients, showing a penetrant role of miRNAs in cell response to hypoxia and in ischaemic cardiovascular diseases. These experiments indicate miRNA as an emerging class of therapeutic targets.
- Published
- 2016
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22. p75(NTR)-dependent activation of NF-κB regulates microRNA-503 transcription and pericyte-endothelial crosstalk in diabetes after limb ischaemia.
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Caporali A, Meloni M, Nailor A, Mitić T, Shantikumar S, Riu F, Sala-Newby GB, Rose L, Besnier M, Katare R, Voellenkle C, Verkade P, Martelli F, Madeddu P, and Emanueli C
- Subjects
- Animals, Cells, Cultured, Diabetes Mellitus, Experimental genetics, Gene Expression Regulation physiology, Hindlimb blood supply, Humans, Ischemia, Male, Mice, Mice, Knockout, MicroRNAs genetics, NF-kappa B genetics, NF-kappa B metabolism, Nerve Tissue Proteins genetics, Oligonucleotide Array Sequence Analysis, Pericytes physiology, Receptors, Nerve Growth Factor genetics, Transcription, Genetic physiology, Diabetes Mellitus, Experimental metabolism, Endothelial Cells metabolism, MicroRNAs metabolism, Nerve Tissue Proteins metabolism, Receptors, Nerve Growth Factor metabolism
- Abstract
The communication between vascular endothelial cells (ECs) and pericytes in the microvasculature is fundamental for vascular growth and homeostasis; however, these processes are disrupted by diabetes. Here we show that modulation of p75(NTR) expression in ECs exposed to high glucose activates transcription of miR-503, which negatively affects pericyte function. p75(NTR) activates NF-κB to bind the miR-503 promoter and upregulate miR-503 expression in ECs. NF-κB further induces activation of Rho kinase and shedding of endothelial microparticles carrying miR-503, which transfer miR-503 from ECs to vascular pericytes. The integrin-mediated uptake of miR-503 in the recipient pericytes reduces expression of EFNB2 and VEGFA, resulting in impaired migration and proliferation. We confirm operation of the above mechanisms in mouse models of diabetes, in which EC-derived miR-503 reduces pericyte coverage of capillaries, increased permeability and impaired post-ischaemic angiogenesis in limb muscles. Collectively, our data demonstrate that miR-503 regulates pericyte-endothelial crosstalk in microvascular diabetic complications.
- Published
- 2015
- Full Text
- View/download PDF
23. Deep-sequencing of endothelial cells exposed to hypoxia reveals the complexity of known and novel microRNAs.
- Author
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Voellenkle C, Rooij Jv, Guffanti A, Brini E, Fasanaro P, Isaia E, Croft L, David M, Capogrossi MC, Moles A, Felsani A, and Martelli F
- Subjects
- Carboxypeptidases metabolism, Cell Hypoxia, Cell Proliferation, Gene Expression Profiling, Gene Expression Regulation, Gene Library, HEK293 Cells, Humans, MicroRNAs metabolism, Molecular Sequence Annotation, Nucleic Acid Conformation, RNA, Double-Stranded, Sequence Analysis, RNA, Signal Transduction, Endothelial Cells metabolism, High-Throughput Nucleotide Sequencing, MicroRNAs analysis, MicroRNAs chemistry
- Abstract
In order to understand the role of microRNAs (miRNAs) in vascular physiopathology, we took advantage of deep-sequencing techniques to accurately and comprehensively profile the entire miRNA population expressed by endothelial cells exposed to hypoxia. SOLiD sequencing of small RNAs derived from human umbilical vein endothelial cells (HUVECs) exposed to 1% O₂ or normoxia for 24 h yielded more than 22 million reads per library. A customized bioinformatic pipeline identified more than 400 annotated microRNA/microRNA* species with a broad abundance range: miR-21 and miR-126 totaled almost 40% of all miRNAs. A complex repertoire of isomiRs was found, displaying also 5' variations, potentially affecting target recognition. High-stringency bioinformatic analysis identified microRNA candidates, whose predicted pre-miRNAs folded into a stable hairpin. Validation of a subset by qPCR identified 18 high-confidence novel miRNAs as detectable in independent HUVEC cultures and associated to the RISC complex. The expression of two novel miRNAs was significantly down-modulated by hypoxia, while miR-210 was significantly induced. Gene ontology analysis of their predicted targets revealed a significant association to hypoxia-inducible factor signaling, cardiovascular diseases, and cancer. Overexpression of the novel miRNAs in hypoxic endothelial cells affected cell growth and confirmed the biological relevance of their down-modulation. In conclusion, deep-sequencing accurately profiled known, variant, and novel microRNAs expressed by endothelial cells in normoxia and hypoxia.
- Published
- 2012
- Full Text
- View/download PDF
24. ChIP-on-chip analysis of in vivo mutant p53 binding to selected gene promoters.
- Author
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Dell'Orso S, Fontemaggi G, Stambolsky P, Goeman F, Voellenkle C, Levrero M, Strano S, Rotter V, Oren M, and Blandino G
- Subjects
- Amino Acid Sequence, Binding Sites genetics, Cell Line, Tumor, Chromatin metabolism, Chromatin Immunoprecipitation, Consensus Sequence genetics, DNA-Binding Proteins genetics, Humans, Mutant Proteins genetics, NF-kappa B metabolism, Protein Binding physiology, Transcription Factors genetics, Transcription Factors metabolism, Transcription, Genetic genetics, Tumor Suppressor Protein p53 genetics, p300-CBP Transcription Factors metabolism, DNA-Binding Proteins metabolism, Mutant Proteins metabolism, Oligonucleotide Array Sequence Analysis, Promoter Regions, Genetic, Tumor Suppressor Protein p53 metabolism
- Abstract
Growing evidence shows that mutant p53 proteins, which are present in many human tumors, gain oncogenic activities that can actively contribute to tumorigenesis. Mutant p53 proteins have been extensively shown to affect the expression of several genes involved in various aspects of cancer biology. We show here the ChIP-on-chip analysis of mutant p53 binding to a set of 154 promoters, composed of both validated and putative mutant p53 target genes. By using the chromatin obtained from mutant p53R175H-immunoprecipitation in proliferating SKBr3 breast cancer cells, we found that mutant p53 binds to 40 of the 154 promoters analyzed. siRNA-mediated mutant p53 knock-down modulates the transcript abundance of some of these target genes. Two-thirds of the mutant p53-bound promoters were also engaged by either p300 or PCAF acetyl-transferases, strongly indicating the presence of transcriptionally active complexes. We also found that NF-kB binding sites are overrepresented among the mutant p53-bound promoters; a ChIP-on-chip analysis confirmed that NF-kB p65 binds to 27 of the mutant p53-bound promoters, indicating that mutant p53 could influence the transcriptional output of these NF-kB target genes.
- Published
- 2011
- Full Text
- View/download PDF
25. MicroRNA signatures in peripheral blood mononuclear cells of chronic heart failure patients.
- Author
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Voellenkle C, van Rooij J, Cappuzzello C, Greco S, Arcelli D, Di Vito L, Melillo G, Rigolini R, Costa E, Crea F, Capogrossi MC, Napolitano M, and Martelli F
- Subjects
- Aged, Aged, 80 and over, Animals, Blood Cells metabolism, Blood Cells pathology, Case-Control Studies, Chronic Disease, Female, Gene Expression Profiling, Heart Failure metabolism, Humans, Leukocytes, Mononuclear pathology, Male, Mice, MicroRNAs metabolism, Middle Aged, Validation Studies as Topic, Heart Failure genetics, Leukocytes, Mononuclear metabolism, MicroRNAs genetics
- Abstract
MicroRNAs (miRNAs) are noncoding RNAs that act as negative regulators of gene expression. Interestingly, specific alterations of miRNA expression have been found in failing hearts of different etiologies. The aim of this study was to identify the miRNA expression pattern of peripheral blood mononuclear cells (PBMCs) derived from chronic heart failure (CHF) patients affected by ischemic (ICM) and nonischemic dilated (NIDCM) cardiomyopathy. The expression profile of 257 miRNAs was assessed in 7 NIDCM patients, 8 ICM patients, and 9 control subjects by quantitative real-time PCR. Significantly modulated miRNAs were validated by using an independent set of 34 CHF patients (NIDCM = 19, ICM = 15) and 19 control subjects. Three miRNAs (miR-107, -139, and -142-5p) were downmodulated in both NIDCM and ICM patients versus control subjects. Other miRNAs were deregulated in only one of the CHF classes analyzed compared with control subjects: miR-142-3p and -29b were increased in NIDCM patients, while miR-125b and -497 were decreased in ICM patients. Bioinformatic analysis of miRNA predicted targets and of gene expression modifications associated with CHF in PBMCs indicated a significant impact of the miRNA signature on the transcriptome. Furthermore, miRNAs of both the NIDCM and the ICM signature shared predicted targets among CHF-modulated genes, suggesting potential additive or synergistic effects. The present study identified miRNAs specifically modulated in the PBMCs of NIDCM and ICM patients. Intriguingly, most of these miRNAs were previously reported as deregulated in human and/or mouse failing hearts. The identified miRNAs might have a potential diagnostic and/or prognostic use in CHF.
- Published
- 2010
- Full Text
- View/download PDF
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