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Background It is very difficult to contain persistently high incidence and mortality rates of gastric cancer. Moreover, the age at onset of gastric cancer is becoming earlier. However, there are few studies on the prediction of the age at gastric cancer onset. Objective To investigate the relationship between single nucleotide polymorphisms (SNPs) in mitochondrial displacement loop (D-Loop) and age at gastric cancer onset. Methods A total of 150 patients with confirmed gastric cancer by pathologically examination of biopsy samples taken during gastroscopy were recruited from Department of Gastroenterology, the Fourth Hospital of Hebei Medical University between July 2007 and December 2008. PCR was used to amplify the targeted fragment of peripheral blood mitochondrial DNA, and the mitochondrial D-Loop region was sequenced. Survival curves for patients with different ages of onset of gastric cancer were plotted by the Kaplan-Meier method, and compared by the Log-rank test. Cox regression analysis was used to identify factors associated with age of onset of gastric cancer. Results The age of onset of patients with different degrees of education was statistically significant (P

Aiming at the problems of low single-seed rate, high rate of missing seeds, high rate of multiple seeds, and low rate of upright seeds in the mechanized sowing of garlic seeds, this paper proposes a structural design scheme of a type hole wheel garlic seed metering device based on the characteristics of garlic species. Based on the agronomic requirements of garlic planting and the characteristics of garlic species, the mechanism of the hole wheel structure to improve the seed filling performance of the seed metering device was clarified, a structural parameter design of the key components of the device was established through theoretical calculations, and a single-factor simulation test was conducted using the Engineering Discrete Element Modelling (EDEM) software. Using the Design-Expert 11 data analysis software, a mathematical regression model of each test factor and test index was established, and the test factors were analyzed. The parameters were optimized, and the following was found to be the optimum parameter combination: seeding wheel speed of 30 rpm, profile hole depth of 18 mm, and adjustment length of the profile hole of 41 mm. The EDEM simulation test results showed a single-seed rate of 91.67% and a missing seed rate of 4.17%. The bench test results showed a single-seed rate of 89.2%, a missing seed rate of 5.0%, and a multiple seed rate of 5.8%. The field test results showed a single-seed rate of 88.44%, a multiple seed rate of 4.11%, a missing seed rate of 7.44%, a breakage rate of 1.11%, and an upright rate of 62.36%. The errors between the bench test/field test and the discrete element simulation test results were 2.7% and 3.5%, respectively, which were consistent with the simulation results, thus meeting the requirements of single-seed sowing using the garlic seed metering device. [ABSTRACT FROM AUTHOR]

Phosphorus (P) nonpoint source pollution from soil to water is increasing dramatically, leading to the eutrophication of water bodies. Using amendment materials for P retention in soil is a promising strategy for environmental restoration and nonpoint source pollution management. This strategy has attracted significant attention because of its highly effective P retention. This study reviews management strategies of P nonpoint pollution from soil to water, including the basic P forms and accumulation situation in soil and P loss from soil to water. Recent advances in the use of amendment materials, such as inorganic, organic, and composite amendment materials, to mitigate P pollution from soil to water have also been summarized. Environmental risks of reloss of P retention in soil with different soil properties and water conditions have also been investigated. This review improves the understanding of P nonpoint source pollution from soil to water, providing an innovative perspective for the large-scale application of amendment materials to control water eutrophication. [ABSTRACT FROM AUTHOR]

In order to solve the related problems of mechanical material structure identification, the author proposes a method that integrates intelligent identification technology into mechanical material identification. Intelligent image recognition technology is based on image features, focusing on the main features of each image, and eliminates redundant input information, finds the key information needed, and completes the sorting of image information in stages to form a complete intuitive image. The experimental results show that the proposed method performs well in most cases, reaching 90.8% for coarse classification, 76.6% for medium classification, and 73.4% for fine classification. It is proved that artificial intelligence recognition technology is of great significance for the application of mechanical material structure recognition. [ABSTRACT FROM AUTHOR]

Although antimicrobial peptides (AMPs) have become powerful drug candidates in the post-antibiotic era, but their low protease stability hinders their clinical application. In the present study, the natural sunflower trypsin inhibitor 1 (SFTI-1) binding loop (CTKSIPPIC) was used to design and synthesize a specific anti-proteolytic sequence template ((RX) n W (RX) n CTKSIPPIC (n = 2, 3; X represents A, I, L, V, F, and W)). After several antibacterial, bactericidal, and toxicity tests, RV3 stood out from the variants and had the highest average selectivity index (SI all = 156.03). It is highly stable in serum, varying pH, temperature, and salt ions as well as under high trypsin, pepsin, or papain concentrations. In a mouse skin inflammation model, established by Pseudomonas aeruginosa infection, RV3 could effectively kill the pathogen, promote wound healing, inhibit inflammatory cell infiltration, and inhibit mRNA and protein expression of TNF-α, IL-6, and IL-1β inflammatory factors. The antibacterial mechanisms of RV3 include combining with lipopolysaccharides and increasing cell membrane permeability, leading to cell membrane rupture and death. These findings indicate that RV3 has great potential for the treatment of bacterial infections. [Display omitted] [ABSTRACT FROM AUTHOR]

Early weaning usually causes intestinal disorders, enteritis, and diarrhea in young animals and human infants. Astragalus polysaccharides (APS) possesses anti‐inflammatory activity. To study the anti‐inflammatory mechanisms of APS and its potential effects on intestinal health, we performed an RNA sequencing (RNA‐seq) study in lipopolysaccharide (LPS)‐stimulated porcine intestinal epithelial cells (IPEC‐J2) in vitro. In addition, LPS‐stimulated BALB/c mice were used to study the effects of APS on intestinal inflammation in vivo. The results from the RNA‐seq analysis show that there were 107, 756, and 5 differentially expressed genes in the control versus LPS, LPS versus LPS+APS, and control versus LPS+APS comparison groups, respectively. The results of Kyoto Encyclopedia of Genes and Genomes enrichment analysis indicated that the mitogen‐activated protein kinase (MAPK) and nuclear factor‐κB (NF‐κB) signaling pathways play significant roles in the regulation of inflammatory factors and chemokine expression by APS. Further verification of the above two pathways by using western blot and immunofluorescence analysis revealed that the gene expression levels of the phosphorylated p38 MAPK, ERK1/2, and NF‐κB p65 were inhibited by APS, while the expression of IκB‐α protein was significantly increased (p <.05), indicating that APS inhibits the production of inflammatory factors and chemokines by the inhibition of activation of the MAPK and NF‐κB inflammatory pathways induced by LPS stimulation. Animal experiments further demonstrated that prefeeding APS in BALB/c mice can alleviate the expression of the jejunal inflammatory factors interleukin 6 (IL‐6), IL‐Iβ, and tumor necrosis factor‐α induced by LPS stimulation and improve jejunal villus morphology. [ABSTRACT FROM AUTHOR]

The endotoxin of Gram-negative bacteria threatens the intestinal health of livestock. Ethyl pyruvate (EP) has been shown to regulate intestinal immunity and protect against cell and tissue damage. In this study, it was first verified that EP could reduce the secretion of IL-8, TNF-α, IL-6 and IL-1β in LPS-induced IPEC-J2 cells. Then, we used RNA sequencing (RNA-seq) to analyze the differentially expressed genes (DEGs) of inflammatory factors induced by LPS in IPEC-J2 cells. It was found that LPS induced the upregulation of 377 genes and the downregulation of 477 genes compared to Vehicle; LPS+EP induced the upregulation of 258 genes and the downregulation of 240 genes compared to Vehicle; and LPS+EP induced the upregulation of 373 genes and the downregulation of 188 genes compared to LPS (fold change > 1.5 and FDR < 0.01). Their enrichment pathways included the MAPK signaling pathway, PI3K-Akt signaling pathway, Toll-like receptor signaling pathway, and other pathways. Furthermore, the mRNA level of cytokines associated with inflammation and apoptosis enriched in the MAPK pathway was verified by qRT-PCR. Western blots and immunofluorescence revealed that EP significantly inhibited phosphorylated p38 and phosphorylated-ERK1/2 protein expression levels (P < 0.05). The apoptosis due to LPS reduced by EP was significantly inhibited, as shown by Annexin V-FITC/PI staining. According to the results, EP inhibited the expression of IL-8, TNF-α, IL-6 and IL-1β as well as apoptosis by inhibiting the phosphorylation of p38 and ERK1/2 in LPS-induced IPEC-J2 cells. [ABSTRACT FROM AUTHOR]

According to the agronomic requirements of garlic sowing, the garlic morphology is studied and a garlic seed metering mechanism with excellent seeding performance is designed. Based on this design, a new garlic seeding machine with an adjustable-size seeding device is developed to realize efficient single-seed metering and seeding of different varieties of garlic. Further, the design scheme of the garlic seeder prototype is established, with the key components of the garlic seeding being designed on the basis of the garlic seeding mechanism. To achieve garlic single-seed metering for different varieties of garlic, the optimal adjustment size of the garlic seed metering device is determined through discrete element simulation analysis. A field experiment confirms the effectiveness of applying the proposed garlic planter to field sowing in terms of the metrics of missing seed and multiple seed rates. The results of the discrete element simulation test reveal that an adjustment size of 40 mm yields the best single-seed metering performance. At an operating speed of 15–35 rpm, the metering device can achieve more than an 80% qualification rate of single-seed metering, with a unit speed of 0.628–1.465 m/s. Thus, the developed garlic seeding device meets the requirements of precision sowing in China and can effectively realize the mechanized planting of garlic. [ABSTRACT FROM AUTHOR]

The therapeutic application of antimicrobial peptides (AMPs), a potential type of peptide-based biomaterial, is impeded by their poor antimicrobial activity and potential cytotoxicity as a lack of understanding of their structure–activity relationships. In order to comprehensively enhance the antibacterial and clinical application potency of AMPs, a rational approach was applied to design amphiphilic peptides, including head-to-tail cyclic, linear and D-proline antimicrobial peptides using the template (IR)nP(IR)nP (n = 1, 2 and 3). Results showed that these amphiphilic peptides demonstrated antimicrobial activity in a size-dependent manner and that cyclic peptide OIR3, which contained three repeating units (IR)3, had greater antimicrobial potency and cell selectivity than liner peptide IR3, DIR3 with D-Pro and gramicidin S (GS). Surface plasmon resonance and endotoxin neutralization assays indicated that OIR3 had significant endotoxin neutralization capabilities, which suggested that the effects of OIR3 were mediated by binding to lipopolysaccharides (LPS). Using fluorescence spectrometry and electron microscopy, we found that OIR3 strongly promoted membrane disruption and thereby induced cell lysis. In addition, an LPS-induced inflammation assay showed that OIR3 inhibited the pro-inflammatory factor TNF-α in RAW264.7 cells. OIR3 was able to reduce oxazolone-induced skin inflammation in allergic dermatitis mouse model via the inhibition of TNF-α, IL-1β and IL-6 mRNA expression. Collectively, the engineered head-to-tail cyclic peptide OIR3 was considerable potential candidate for use as a clinical therapeutic for the treatment of bacterial infections and skin inflammation. [ABSTRACT FROM AUTHOR]

Antibacterial peptides (APMs) are a new type of antibacterial substance. The relationship between their structure and function remains indistinct; in particular, there is a lack of a definitive and fixed template for designing new antimicrobial peptides. Previous studies have shown that porcine Protegrin-1 (PG-1) exhibits considerable antimicrobial activity and cytotoxicity. In this study, to reduce cytotoxicity and increase cell selectivity, we designed histidine-rich peptides based on the sequence template RR(XY)2XDPGX(YX)2RR-NH2, where X represents I, W, V, and F. The results showed that the peptides form more β-hairpin structures in a lipid-rich environment that mimics cell membranes. Among them, the antimicrobial peptide HV2 showed strong antibacterial activity against Gram-negative strains and almost no toxicity to normal cells. The results of our analysis of its antibacterial mechanism showed that peptide HV2 acts on the bacterial cell membrane to increase its permeability, resulting in cell membrane disruption and death. Furthermore, peptide HV2 inhibited bacterial movement in a concentration-dependent manner and had a more robust anti-inflammatory effect by inhibiting the production of TNF-α. In summary, peptide HV2 exhibits high bactericidal activity and cell selectivity, making it a promising candidate for future use as an antibiotic. [ABSTRACT FROM AUTHOR]

Salmonella typhimurium is one of the main causes of intestinal diseases, affecting the health of humans and livestock. Ethyl pyruvate (EP), which is ordinarily an edible spice, has been indicated to exert anti-inflammatory effects and preserve intestinal barrier function. In this study, intestinal immune function and signaling pathways activated by EP were investigated in vivo in S. typhimurium- challenged BALB/c mice and in vitro in RAW264.7 cells. EP improved body weight loss and the organ index of the liver and spleen (p < 0.05). Serum IgA and IgM levels were significantly increased in EP-treated mice (p < 0.05). According to histopathological and immunohistochemical staining, EP significantly increased the villus height, reduced edema in the jejunum and increased the levels of claudin-1, occludin-1 and ZO-1 proteins compared to the Salmonella -treated group (p < 0.05). In addition, EP decreased the levels of the IL-6, IL-1β and TNF-α mRNA levels in jejunum, liver, spleen and RAW264.7 cells (p < 0.05). EP decreased the levels of TLR4, phosphorylated p38MAPK and ERK1/2 in mice infected with S. typhimurium (p < 0.05). In conclusion, EP effectively protected BALB/c mice from an intestinal S. typhimurium infection by improving the activity of the humoral immune system, reducing intestinal barrier damage, and inhibiting proinflammatory cytokine production in the jejunum by modulating the TLR4/MAPK signaling pathway. Based on these findings, EP has the potential to inhibit inflammation or to serve as an immune-enhancing adjuvant. [ABSTRACT FROM AUTHOR]

Salmonella typhimurium (S. t.) is one of the main pathogens that causes acute gastroenteritis. To evaluate the anti-inflammatory mechanism of Astragalus polysaccharide (APS) in vivo and its influence on the intestinal flora, BALB/c mice were infected with S. t. to establish a model of diarrhea. The disease activity index (DAI) scores showed that APS attenuated S. t.-induced weight loss and diarrhea in mice. APS significantly reduced the index of the liver and spleen as well as the ALT and AST levels in serum (P < 0.05). Hematoxylin and eosin (H&E) results indicated that APS significantly increased jejunum villus height and crypt depth and reduced the infiltration of inflammatory cells (P < 0.05). Additionally, APS increased the tight junction (TJ) proteins expression levels of ZO-1, Occludin and Claudin-1 in the jejunum. The results of 16S rDNA showed that APS significantly increased the number of Lactobacillus and Bifidobacterium spp. to normal levels (compared with the control group). In addition, APS significantly decreased the mRNA expression levels of the proinflammatory cytokines TNF-α, IL-1β, IL-6 and IL-17 in the jejunum (P < 0.01) as well as the proteins expression levels of COX-2 and iNOS (P < 0.05). Western blot confirmed that prefeeding with APS inhibited S. t. -induced expression of TLR4 and MyD88 in the jejunum and further inhibited nuclear factor-κB (NF-κB) activation, including the nuclear translocation of the p65 NF-κB subunit and the phosphorylation and degradation of IκB-α. This was the key to APS inhibition of the production of inflammatory factors and inflammatory mediators in the jejunum. • In our study, APS relieved inflammation through inhibiting body weight loss and reducing the liver and spleen index of infected mice. • APS increased the intestinal barrier function by repairing damaged structures of the jejunum villi and balancing intestinal flora. • PS is expected to be an alternative to antibiotics for the treatment of intestinal inflammation caused by bacterial infections. [ABSTRACT FROM AUTHOR]

The application of autonomous navigation technology of electric crawler tractors is an important link in the development of intelligent greenhouses. Aiming at the characteristics of enclosed and narrow space and uneven ground potholes in greenhouse planting, to improve the intelligence level of greenhouse electric crawler tractors, this paper develops a navigation system of electric crawler tractors for the greenhouse planting environment based on LiDAR technology. The navigation hardware system consists of five modules: the information perception module, the control module, the communication module, the motion module, and the power module. The software system is composed of three layers: the application layer, the data processing layer, and the execution layer. The developed navigation system uses LiDAR, Inertial Measurement Unit (IMU) and wheel speed sensor to sense the greenhouse environment and the crawler tractor's information, employs the Gmapping algorithm to build the greenhouse environment map, and utilizes the adaptive Monte Carlo positioning algorithm for positioning. The simulation test of different global path planning algorithms in Matlab shows that the A* algorithm obtains the optimal overall global path. In the scene of map 5, the path planned by the A* algorithm is the most significant, and the number of inflection points is reduced by 40.00% and 87.50%, respectively; meanwhile, the path length is the same as that of the Dijkstra algorithm, but the runtime is reduced by 68.87% and 81.49%, respectively; compared with the RRT algorithm, the path length is reduced by 7.27%. Therefore, the A* algorithm and the Dynamic Window Approach (DWA) method are used for tractor navigation and obstacle avoidance, which ensures global path optimality while also achieving effective local path planning for obstacle avoidance. The test results suggest that the maximum lateral deviation of the built map is 6 cm, and the maximum longitudinal deviation is 16 cm, which meets the requirement of map accuracy. Additionally, the results of the navigation accuracy test indicate that the maximum lateral deviation of navigation is less than 13 cm, the average lateral deviation is less than 7 cm, and the standard lateral deviation is less than 8 cm. The maximum heading deviation is less than 14°, the average heading deviation is less than 7°, and the standard deviation is less than 8°. These results show that the developed navigation system meets the navigation accuracy requirements of electric crawler tractors in the greenhouse environment., Competing Interests: Author JZ was employed by the company BYD Automobile Company Limited. Authors YH and SG were employed by the company Xi ‘an Dongyang Machinery Company Limited. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.​, (Copyright © 2024 Guo, Li, Wang, Wang, Rong, Wang, Wang, Wang, Zhang, Huo and Guo.)