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ObjectiveTo understand the current drug resistance status and bacterial multilocus sequence typing （MLST） of human and avian Campylobacter jejuni in Jinshan District， Shanghai.MethodsFecal samples were collected from diarrhea patients in the annuity mountainous area from 2021 to 2022， and poultry and related samples were collected from 2 poultry farms in the Jinshan area for detection of C. jejuni. Minimal inhibitory concentration （MIC） drug sensitivity test was performed on the detected C. jejuni， and some strains were selected for whole genome sequencing and MLST analysis.ResultsA total of 823 samples of diarrhea disease were collected， and 32 strains of C. jejuni were detected， with a detection rate of 3.89%. Out of 600 poultry related samples， 62 strains of C. jejuni were detected， with a detection rate of 10.33%. Human multidrug resistance reached 93.75% （30/32）， while avian multidrug resistance reached 100.00%（62/62）. The top four drug resistance rates of human and avian C. jejuni were azithromycin （100.00% from humans and 100.00% from birds）， naphthoic acid （93.75% from humans and 87.10% from birds）， ciprofloxacin （90.63% from humans and 98.39% from birds）， and tetracycline （84.38% from humans and 98.39% from birds）. The relatively low resistance strains of human derived C. were erythromycin， chloramphenicol， and thalithromycin. The relatively low resistance strains of avian C. jejuni were erythromycin， clindamycin， and flufenicol. MLST analysis showed that the selected 16 strains of bacteria were divided into 9 ST types， among which the evolutionary relationship of avian C. jejuni was relatively concentrated， while human C. jejuni was relatively dispersed. It was found that one strain of avian C. jejuni was closely related to two strains of human C. jejuni.ConclusionsC. jejuni infection is severe in patients with diarrhea in this region， with a detection rate second only to salmonella and Vibrio parahaemolyticus. C. jejuni infection in poultry is relatively common， and both are highly resistant. Therefore， monitoring and control should be strengthened. MLST analysis shows new ST types in both avian and human sources of C. jejuni， indicating the emergence of new mutations that require continuous monitoring to avoid the epidemics caused by new strains. The isolated strains with close genetic relationships between avian and human sources reveal the evidence of the spread of C. jejuni from poultry to humans. Therefore it is necessary to strengthen the monitoring of C. jejuni in relevant samples from breeding farms.

Diabetic retinopathy has a serious impact on the quality of life of diabetes patients. In clinic, anti-vascular endothelial growth factor, laser or surgical treatment are mainly used, which has limited therapeutic effect, so it is urgent to update more effective prevention and treatment methods. The Hippo signaling pathway is a complex kinase chain that includes a series of kinases and transcription factors involved in regulating processes such as cell proliferation, differentiation, and apoptosis. It has been proved that there is a typical Hippo signaling pathway in the human retina, and its main influencing factor YAP/TEA regulates the process of retinal endothelium and angiogenesis. This article reviews the composition and regulatory mechanism of Hippo signaling pathway, the relationship between Hippo signaling pathway and other signaling pathways and risk factors in diabetic retinopathy, and related drug treatment, in order to provide a broader idea for the prevention and treatment of diabetic retinopathy.

High myopia is a highly population-specific and genetically heterogeneous eye disease for which there is no effective treatment. Myopia is more prevalent and severe in east Asians, especially in China. Prevention and control of myopia and preclinical myopia can help prevent its progression to high myopia. Therefore, numerous studies on molecular genetics and environmental factors have been conducted to identify genes associated with high myopia in order to establish a polygenic risk assessment for myopia and to elucidate the pathogenesis and pathway mechanisms. This paper will discuss the recent advances in genetic loci, risk factors, and myopia prevention and control tools based on modern medicine and molecular genetics for high myopia-related genes.

Objective To investigate the efficacy and safety of fractional micro ablative laser combined with estriol cream in the treatment of postmenopausal mild to moderate stress urinary incontinence（SUI）. Methods Postmenopausal patients（220 cases） with mild to moderate SUI in Sichuan Jinxin Women & Children Hostipal and the Third People's Hospital of Chengdu from January 2020 to January 2022 were randomly divided into the control group（n=110） and the study group（n=110）. The patients in the control group were treated with fractional micro ablative laser, while the patients in the study group was additionally given estriol cream. The patients were followed up for 6 months. The clinical efficacy, 1h urine leakage, urinary incontinence symptom score, quality of life score, selfrating anxiety scale（SAS）, selfrating depression scale（SDS） score, endometrial thickness changes and adverse reactions were compared between two groups. Results One patient was lost to followup in control group. Compared with control group, the total effective rate was higher（84.55% vs 73.39%, P＜0.05）, the 1h urine leakage was less ［（1.96±0.78）g vs （2.56±1.14）g， P＜0.05］, the quality of life score was higher, the scores of urinary incontinence symptoms, SAS and SDS were lower （P＜0.05） in the study group. There was no significant difference in the incidence of adverse reactions and endometrial thickness during treatment and followup （P＞0.05）. Conclusion Fractional micro ablative laser combined with estriol cream in the treatment of mild to moderate postmenopausal SUI can improve the effective rate and the quality of life of patients, relieve anxiety and depression, have long treatment intervals, convenience and high safety.

Highlights Wood plastic composite (WPC) is a kind of eco‐friendly material made of agricultural and forest industry waste and residues compounded with thermoplastic. The defects of traditional WPC, such as low strength and high creep, greatly limit its engineering applications. To solve the problem, this paper proposed two methods of glass fiber reinforced polymer (GFRP) reinforced PVC‐based WPC panels (G‐WPC) by bonding GFRP sheets or embedding GFRP bars in the tensile zone of the WPC panels. The effects of the thickness of GFRP sheet and reinforcement ratio of GFRP bar on the flexural property of G‐WPC were comparatively analyzed by carrying out four‐point bending tests and finite element simulations. The results showed that the failure modes of the GFRP sheets reinforced specimens were mainly flexural fracture and interface debonding, and the GFRP bars reinforced specimens were flexural fracture and excessive deformation. The ultimate flexural bearing capacity of both GFRP sheets/bars reinforced specimens could be improved by more than 200%. GFRP bar reinforced specimens had better ductility than reinforced with sheet. The effects of WPC density, GFRP sheet fiber layup angle and GFRP bar diameter on the flexural behavior of G‐WPC were further parametrically analyzed using the finite element model (FEM). Two effective flexural reinforcement methods of WPC were proposed. The optimum thickness of GFRP sheet was obtained based on experimental tests. The suitable reinforcement ratio of GFRP bars was studied by tests and FEM. Effects of WPC density, GFRP fiber layup angle and bar diameter were analyzed. [ABSTRACT FROM AUTHOR]