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Aerobic rice is a new production system in which specially-developed varieties are grown under non-flooded, non-puddled, and non-saturated soil conditions. In 2003–2004, irrigation x Nitrogen experiments were carried out near Beijing using variety HD297. Water treatments included four irrigation levels, and Nitrogen treatments included different fertilizer N application rates and different numbers of N splits. The highest yields were 4 460 kg/ha with 688 mm of total (rain plus irrigation) water input in 2003 and 6 026 kg/ha with 705 mm of water input in 2004. Because of the quite even distribution of rainfall in both years, the four irrigation treatments did not result in large differences of soil water conditions. There were few significant effects of irrigation on biomass accumulation, but yield increased with the total amount of water applied. High yields coincided with high harvest index and high percentages of grain filling. The application of fertilizer N either reduced biomass and yield or kept it at the same level as 0 N and consistently reduced the percentage grain filling and 1000-grain weight. With the highest water application, five splits of N gave higher yield than three splits, whereas three splits gave higher yield than five splits with lower water applications. [ABSTRACT FROM AUTHOR]

Drought is the greatest agro-meteorological disaster. Risk assessment of drought is of great guiding significance to improve the regional risk management and decision-making level, and to further reduce agricultural loss. In this paper, drought risk of summer maize was analyzed accor-ding to natural disaster risk theory, using the data of weather, planting area and yield of summer maize, and local effective irrigation area of 69 stations in Huanghuaihai area. The assessing indices and models of drought risk were established from the aspects of danger, vulnerability, exposure, and disaster prevention and mitigation capacity. The results showed that, in Huanghuaihai area, the most dangerous periods for drought during summer maize season (June-September) were the follo-wing two stages: sowing to emergence and milky to maturity. Spatially, the highest drought risk was mainly distributed in central and southern Hebei, western and northern Henan. After weighting the vulnerability, exposure, and disaster prevention and mitigation capacity, the integrated highest drought risk was mainly distributed in the west and southwest of Henan Province. The sub-high risk area was distributed in the south of Henan, Cangzhou and Xingtai of Hebei, and Dezhou of Shandong. The lowest risk area was located in south of Shandong, north of Anhui, and Xinyang of Henan. The other areas were at moderate risk.

The fabrication of micropatterned structures on PDMS is a critical step in soft lithography, microfluidics, and many other PDMS-based applications. To substitute traditional mold-casting methods, we develop a simple method to create micropatterned nanostructures on PDMS in one step. After exposing a flat PDMS surface to a UV pen lamp through a photomask (such as a TEM grid), micropatterned nanostructures can be formed readily on the PDMS surface. We also demonstrate that fabricated PDMS can be used for the microcontact printing of protein immunoglobulin (IgG) on solid surfaces. This method is probably the simplest method of creating micropatterned nanostructures on PDMS reported so far because it does not need casting, surface coating, or chemical reagents. Only a UV pen lamp and a photomask are required, and this method can be performed under ambient conditions without vacuum. We expect that this method will greatly benefit researchers who use PDMS regularly in various applications such as soft lithography and microfluidics.

In this paper, we report the role of surfactants in minimizing nonspecific protein adsorption in liquid crystal (LC)-based immunoassays in which LC is used as a readout system. Among all surfactants tested, only nonionic surfactant such as Tween 20 can effectively reduce the nonspecific protein adsorption, while maintaining the selectivity of the LC-based immunoassay. We also show that to minimize nonspecific protein adsorption, Tween 20 can be added directly into the antibody solution to a final concentration of 0.8 mM. After the addition of Tween 20, better correlations between the antibody concentrations and the interference colors of LCs can therefore be obtained. For example, when Cy3 antibiotin was used, black, yellow, red, and green interference colors correspond to a concentration of 5, 25, 50, and 100 μg/mL, respectively. This feature gives LC immunoassay a unique advantage over the fluorescence-based immunoassay.

We report a simple method of creating well-defined micropatterns on the surface of a flat PDMS stamp, making it suitable for microcontact printing of proteins. This method only requires a UV lamp (254 nm) and a TEM grid (as a photomask) to modify the surface of PDMS for creating desired micropatterns. By using the UV-modified stamp, a printed protein micropattern that resembles the original TEM grid can be obtained. Surprisingly, unlike the oxygen-plasma-treated PDMS, the UV-modified flat stamp is also long-lasting (>1 week). The method reported herein is very economical for microcontact printing applications because expensive silicon masters and microstructured PDMS are no longer required.

Chemically micropatterned surfaces have broad applications in many fields. In this paper, we report a new method for preparing chemically micropatterned surfaces by controlling and manipulating supported phospholipid monolayers as soft resist layers with molecular-level precision. First, we introduce self-assembled supported phospholipid monolayers on solid surfaces and use a microcontact lift-up process to create micropatterned phospholipid monolayers (with micrometer resolution) on the surface. Next, the micropatterned phospholipid monolayers can function as "soft" resist layers to protect underlying solid substrates and create either positive or negative chemically micropatterned surfaces during subsequent treatments. Unlike traditional "hard" resist layers which can only be removed by using harsh chemical treatments, this novel soft resist layer only comprises a single layer of compact phospholipid; therefore, it can be easily removed by water rinsing after the preparation of micropatterns. This method is also versatile. It can be applied to prepare a protein microarray or silver patterns on solid substrates.

Protein assays are critical analytical tools performed in various biochemical laboratories to quantify the concentration of proteins. In this study, we report the optical responses of a thin layer of liquid crystals supported on glass slides decorated with proteins and the utility of this phenomenon as a new "all-or-nothing" type of protein assay. It was found that the orientations of liquid crystals are very sensitive to the concentration of protein solution applied to the surface. When the protein concentration exceeds a critical value (IgG 5.0 microg/mL, BSA 6.0 microg/mL, FTIC-anti-biotin 0.40 microg/mL, and FITC-anti-IgG 0.37 microg/mL), the thin layer of liquid crystals gives a very sharp dark-to-bright optical response within a small concentration range. This characteristic is not observed in any traditional protein assays, which are based on the adsorption of UV or visible light. The optical response is also very precise and reproducible. It is not affected by the thickness of the liquid crystal cell or the amount of organosilanes coated on the glass slides. The liquid crystal-based protein assay may be very useful for screening purposes, especially when a simple positive or negative answer is desired.

In this paper we present a study of using oxygen plasma for chemically modifying inert hydrocarbon self-assembled monolayers of octadecyltrichlorosilane (OTS-SAMs) and rendering active surfaces for protein immobilization. Detailed surface modification and protein immobilization were characterized by using ellipsometry, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared-attenuated total reflectance spectroscopy, and fluorescence microscopy. Our XPS results showed that the surface reaction between OTS-SAMs and oxygen plasma can generate new surface functional groups such as alcohol (C-O), aldehyde (C=O), and carboxylic acid (O-C=O), and their compositions can be controlled by using different treatment times and powers. A short treatment time ( approximately 1 s) and high power (10 W) can lead to a higher density of aldehyde groups, which can serve as linker groups for protein immobilization through the formation of Schiff bases with the amine groups of proteins. By using the fluorescence immunostaining method, we confirmed that human immunoglobulin (IgG) can be immobilized on a glass slide, only if the surface was decorated with OTS-SAMs and if the OTS-SAMs were pretreated with oxygen plasma. The protein immobilized on the oxygen-plasma-treated surface can only be recognized by using a highly specific antibody, FITC-anti-IgG, but not FITC-anti-biotin.