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Through graded salting out with ammonium sulfate and dialysis, esterases were extracted and purified from goose liver, chicken liver and pig liver respectively, then their structures were characterized, and their inhibition responses to carbaryl as a pesticide model were further investigated. Results showed that the yield of goose liver esterase was the highest (14.58%), and that of chicken liver esterase was the lowest (8.82%) with the best thermal stability, while the protein mass concentration of pig liver esterase was the highest (12.47mg/mL); the protein molecular weights of all three kinds of animal liver esterases were 30 ~ 110 kDa with the leading one of 55 kDa, and that in chicken liver esterase was the highest (16.74%) while the lowest was in that of pig liver esterase (14.31%); these three kinds of animal liver esterases showed different sizes of protein particles with flake structures and smooth surfaces; in the carbaryl concentration range of 0.5~10.0 mg/L, the linear range (0.5~5.0 mg/L), the detection limit (0.29 mg/L) and IC50 (7.74 mg/L) of chicken liver esterase were the lowest, which could be used as a potential sensitive enzyme source for rapid detection of carbaryl. [ABSTRACT FROM AUTHOR]