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Purpose: In essential hypertensive patients, cardiac remodeling may be associated with the risk of renal damage in the future which can be reflected by the estimated glomerular filtration rate (eGFR). Through retrospective analysis, we evaluated the potential of cardiac remodeling based on echocardiographic measurements to predict the risk of renal damage in the future with hypertensive patients. Methods: We retrospectively analyzed the relationship between the changes of left heart structure and function and renal damage for 510 patients with hypertension, who were diagnosed between 2016 to 2022. Demography data, clinical data, blood samples and echocardiographic variables were used for survival analysis, and the Cox proportional hazards regression model was used. Results: In our study, we found that age, serum creatinine (SCR), creatine kinase isoenzyme MB (CK MB), abnormal high-sensitivity troponin I (TNI), interventricular septum thickness (IVST) and left ventricular ejection fraction (LVEF) could be used as independent predictors in risk of renal impairment in hypertensive patients (p< 0.05). Combined in a score where one point was given for the presence of each of the parameters above, this score could strongly predict renal function damage in the future (p< 0.05). In receiver operating characteristics (ROC) curve analyses, the area under the curve of the risk factor score was 0.849 (P< 0.001). Conclusion: In essential hypertensive patients, LVEF and IVST can predict the risk of future adverse renal outcomes. Moreover, combining risk variables into a simplified score may enable to assess the risk of renal impairment in hypertensive patients at an early stage. [ABSTRACT FROM AUTHOR]

For the analysis of multivariate failure time data when the primary covariates are incomplete but the auxiliary covariates for them are available for the whole cohort subjects, an estimated quadratic inference function method is developed to improve the estimation efficiency under the marginal hazard model with common baseline hazard function. Both the auxiliary information and the intra-cluster correlation between the failure times are incorporated in the estimation procedure. The proposed estimator is shown to be consistent and asymptotically normal. Simulation studies demonstrate that when the intra-cluster correlation is strong or moderate, the proposed method gains noticeable efficiency compared to other existing methods. [ABSTRACT FROM AUTHOR]

Gallic acid (GA) and lecithin showed important roles in antioxidant and drug delivery, respectively. A complex synthesized from GA and soybean lecithin (SL-GAC), significantly improved bioavailability of GA and pharmacological activities. However, the antioxidant activity of SL-GAC and its effect on iron-overload-induced liver injury remains unexplored. This study investigates the antioxidant properties of SL-GAC in vitro and in mice, and its remediating effects against liver injury by iron-overloaded. In vitro, free radical scavenging activity, lipid peroxidation inhibition, and ferric reducing power of SL-GAC were measured by absorbance photometry. In vivo, C57BL/6J mice were randomized into 4 groups: control, iron-overloaded, iron-overloaded + deferoxamine, and iron-overloaded + SL-GAC. Treatments with deferoxamine (150 mg/kg/intraperitioneally) and SL-GAC (200 mg/kg/orally) were given to the desired groups for 12 weeks, daily. Iron levels, oxidative stress, and biochemical parameters were determined by histopathological examination and molecular biological techniques. In vitro, SL-GAC showed DPPH and ABTS free radicals scavenging activity with IC50 values equal to 24.92 and 128.36 μg/mL, respectively. In C57BL/6J mice, SL-GAC significantly reduced the levels of serum iron (22.82%), liver iron (50.29%), aspartate transaminase (25.97%), alanine transaminase (38.07%), gamma glutamyl transferase (42.11%), malondialdehyde (19.82%), total cholesterol (45.96%), triglyceride (34.90%), ferritin light chain (18.51%) and transferrin receptor (27.39%), while up-regulated the levels of superoxide dismutase (24.69%), and glutathione (11.91%). These findings encourage the use of SL-GAC to treat liver injury induced by iron-overloaded. Further in vivo and in vitro studies are needed to validate its potential in clinical medicine. [ABSTRACT FROM AUTHOR]

Building Z-scheme heterojunctions with an electron bridge is a favored function for increasing photocatalytic activity. A facile approach for preparing g-C3N4/Ag@AgCl ternary heterojunctions by co-precipitation and photoreduction was established in this work. First, via co-precipitation, AgCl was modified on the surface of g-C3N4 to create a broad contact area between AgCl and g-C3N4. The AgCl is then reduced to Ag via an in-situ photoreduction technique, resulting in the formation of a ternary composite. The experimental results showed that when g-C3N4 modified 25% of the Ag@AgCl, that is, g-C3N4/Ag@AgCl-25 had the best photocatalytic performance, 94.9% of TC was degraded within 240 min, and the reaction rate to TC was 0.1214 min-1, which was 4.49 times and 8.12 times higher than that of g-C3N4 and Ag/AgCl, respectively. The excellent photocatalytic performance of g-C3N4/Ag@AgCl is attributed to the LSPR effect of Ag NPs and O-doping g-C3N4, which broadens the absorbance performance of g-C3N4, the establishment of Z-type heterojunctions between AgCl NPs and g-C3N4 NSs and Ag NPs as an electron transport bridge accelerate the photogenerated electrons transfer between AgCl and g-C3N4. [ABSTRACT FROM AUTHOR]

We develop the quadratic inference function method to the parameter estimation of the marginal models with general relative risk form and common baseline hazard function for multivariate failure time data. The usual exponential relative risk form is relaxed to an non-negative twice differentiable form. The proposed estimator, under some regularity conditions, is shown to be consistent and asymptotically normal with a covariance matrix that can be consistently estimated. In addition, a test statistic based on the quadratic inference function for the parameter inference has been provided. The simulation results show that the proposed method taking the correlation between the failure times into the estimation procedure gains more efficiency than the method using the independent structure when the correlation cannot be ignored, and can easily deal with the situation when the cluster size is large. The proposed method is illustrated by analysis of a real data from the Diabetic Retinopathy Study. [ABSTRACT FROM AUTHOR]

An estimated quadratic inference function method is proposed for correlated failure time data with auxiliary covariates. The proposed method makes efficient use of the auxiliary information for the incomplete exposure covariates and preserves the property of the quadratic inference function method that requires the covariates to be completely observed. It can improve the estimation efficiency and easily deal with the situation when the cluster size is large. The proposed estimator which minimizes the estimated quadratic inference function is shown to be consistent and asymptotically normal. A chi‐squared test based on the estimated quadratic inference function is proposed to test hypotheses about the regression parameters. The small‐sample performance of the proposed method is investigated through extensive simulation studies. The proposed method is then applied to analyze the Study of Left Ventricular Dysfunction (SOLVD) data as an illustration. [ABSTRACT FROM AUTHOR]

In order to study the mechanical properties of ultra-high performance engineered cementitious composites (UHP-ECC) used for cable channel repair, orthogonal tests were carried out with four influencing factors, water binder ratio, silica fume, fly ash and mortar ratio, to obtain the optimum mix ratio of the cement paste. On this basis, the effects of ethylene-vinyl acetate (EVA) polymer and polyvinyl alcohol (PVA) fiber on the fluidity, flexural strength and compressive strength of UHP-ECC were studied, and the micromechanism was analyzed with SEM. The results show that the fluidity of UHP-ECC material prepared was 170–200 mm, which meets the requirements of working performance. The average compressive strength at 28 days reached 85.3 MPa, and the average flexural strength at 28 days reached 22.3 MPa. EVA polymer has a fast film forming rate in an alkaline environment. The formed polymer film wraps the fiber, enhances the bridging role between the fiber and the matrix and increases the viscosity of the material. Therefore, the early flexural strength is significantly improved. The 1-d flexural strength of UHP-ECC material mixed with 9-mm fiber is increased by 18%, and the 1-d flexural strength of 3-mm fiber is increased by 15%. Due to PVA fiber's high elastic modulus and tensile strength, it improved the flexural and tensile properties of the material after incorporation, especially in the later stages; the 28-d flexural strength of UHP-ECC material mixed with 9-mm fiber increased by 12%, and the 28-d flexural strength of 3-mm fiber increased by 7%. It was concluded that the effect of 9-mm PVA fiber is better than that of 3 mm PVA fiber. [ABSTRACT FROM AUTHOR]

Background: The dietary inflammatory index (DII) is thought to be related to many healthy events. However, the association between the DII and sarcopenia remains unclear. Methods: The meta-analysis was conducted to evaluate the effects of the DII on the risk of sarcopenia utilizing available studies. Up to September 2022, Cochrane, PubMed, Web of Science, Medline, and EMBASE databases were searched to evaluate the relationships between the DII and sarcopenia. A random‒effects model was used to calculate the effect size and 95% confidence intervals (CIs). Result: Eleven studies with 19,954 participants were included in our meta-analysis. The results indicated that a high DII increased the risk of sarcopenia (OR = 1.16, 95%CI [1.06, 1.27], p < 0.05). The result of the dose–response analysis showed that the risk of sarcopenia increased by 1.22 times for each 1-point increase in the DII score (OR = 1.22, 95%CI [1.12, 1.33], p < 0.05). Conclusion: The meta-analysis demonstrated that the DII is associated with sarcopenia. Considering some limitations in this study, more studies are needed to verify this relationship. [ABSTRACT FROM AUTHOR]

Urban ground collapse is a common geological disaster characterized by its invisible nature, particularly in China, and results in significant socioeconomic losses and even loss of life. Underground pipeline breakage is the most common factor leading to urban ground collapses. Hence, it is essential to study how different types of pipeline breakages initiate the collapse mechanism. In this study, an indoor model test was conducted to directly observe the process of collapse due to broken pipe leakage. A broken pipe was put into a model box and tested by an experimental device. The results showed that among the different pipeline breakage types, vertical damage had the greatest influence on the degree of cavity development and ground collapse. Similarities were observed in the patterns of cavity evolution development and the extent of ground collapse as well, further revealing the significance of the cavity evolution process in predicting ground collapses. [ABSTRACT FROM AUTHOR]

Simple Summary: Porcine parvovirus disease is an animal disease with a high infection rate that is wide spread in the world. Its spread and variants have a very serious impact on large-scale livestock economy. So far, there have been no reports on the infection and genetics of pig parvovirus in Tibetan pigs. This study was conducted to investigate and analyze the seroprevalence, regional distribution and risk factors responsible for porcine parvovirus in Tibetan pigs in Tibet. This study also contains an effective response and analysis of strategies made in the areas under investigation through the epidemic, distribution and risk factors inquiry of the virus. A total of 356 serum samples were collected from four counties in Tibet, and the anti-porcine parvovirus antibodies were detected by using the competitive ELISA kit. In this investigation, 324 (91.01%) of 356 serum samples were found to be positive for porcine parvovirus. Porcine parvovirus is also extremely widespread in Tibet, reaching a worrying seroprevalence that requires greater awareness of this disease by the operators in the swine sector. Possible measures to improve surveillance and monitoring of the disease should be considered. Porcine parvovirus (PPV) disease is a worldwide spread animal disease with high infection rate and serious impact on meat economy causing significant losses in livestock production. The purpose of this paper is to investigate and analyze the regional seroprevalence of PPV in Tibetan pigs in Tibet and evaluate risk factors related to the disease. A total of 356 serum samples of Tibetan pigs were collected from four counties and districts in Tibet, and anti-PPV antibodies were detected by using a commercial competitive ELISA. Our results show a seroprevalence of 91.01% (324 serum samples were found to be positive for anti-PPV antibodies). The positive rate among different district was 100%, 96.55%, 93.68% and 72.83%, respectively in the Mainling County, in Bayi district, Nang County and Bomê County. We found significant differences between different age and gender groups; particularly female animals show a seroprevalence of 96.03% while the males only 83.46%. From the perspective of the growth stage, our results indicate that subadults show a seroprevalence significative higher than other age groups (100%). This study describes for the first time the PPV seroprevalence among Tibetan pigs characterizing risk factors involved in its transmission and providing information to be taken into account for eventual surveillance or eradication plans. [ABSTRACT FROM AUTHOR]

Cancer has become a major challenge in the global disease burden. Artificial sweeteners are a class of chemical compounds that are used as food and beverage addition agent to replace sugar. However, the health effects of consuming artificial sweeteners are still unclear. This meta-analysis was performed to evaluate the role of artificial sweeteners on cancer. The databases PubMed, Cochrane Library, MEDLINE, Web of Science and EMBASE were searched up until July 2022. A Newcastle–Ottawa scale (NOS) was used to estimate the study quality. A total of 25 observational studies were included with a total of 3,739,775 subjects. The intake of artificial sweeteners had no apparent association with overall cancer incidence and mortality. However, in Europe, artificial sweeteners' intake could increase the risk of cancer incidence (HR/RR = 1.07, 95% CI = [1.02, 1.12], I2 = 25.8%, P = 0.223), which appears to be related to a shift in nutritional behaviors in the countries. Significant results were also observed in subgroups with aspartame and a mixed intake of artificial sweeteners. Moreover, higher risk was observed for artificial sweeteners intake in all-cause mortality (HR/RR =1.13, 95% CI = [1.03, 1.25], I2 = 79.7%, p < 0.001) and a J-shaped association between them was found. More data from well-conducted studies and clinical trials are required. [ABSTRACT FROM AUTHOR]

Deficient bone regeneration causes bone defects or nonunion in a substantial proportion of trauma patients that urges for novel therapies. To develop a reliable therapy, we investigated the effect of negative pressure wound therapy (NPWT) on bone regeneration in vivo in a rat calvarial defect model. Negative pressure (NP) treatment in vitro was mimicked to test its effect on osteoblast differentiation in rat mesenchymal stem cells (MSCs) and MC3T3-E1 cells. Transcriptomic analyses, pharmaceutical interventions, and shRNA knockdowns were conducted to explore the underlying mechanism and their clinical relevance was investigated in samples from patients with nonunion. The potential application of a combined therapy of MSCs in hydrogels with negative pressure was tested in the rat critical-size calvarial defect model. We found that NPWT promoted bone regeneration in vivo and NP treatment induced osteoblast differentiation in vitro. NP induced osteogenesis via activating macroautophagy/autophagy by AMPK-ULK1 signaling that was impaired in clinical samples from patients with nonunion. More importantly, the combined therapy involving MSCs in hydrogels with negative pressure significantly improved bone regeneration in rat critical-size calvarial defect model. Thus, our study identifies a novel AMPK-ULK1-autophagy axis by which negative pressure promotes osteoblast differentiation of MSCs and bone regeneration. NPWT treatment can potentially be adopted for therapy of bone defects. Abbreviations: ADP, adenosine diphosphate; AICAR/Aic, acadesine; ALP, alkaline phosphatase; ALPL, alkaline phosphatase, biomineralization associated; AMP, adenosine monophosphate; AMPK, AMP-activated protein kinase; ARS, alizarin red S staining; ATG7, autophagy related 7; ATP, adenosine triphosphate; BA1, bafilomycin A1; BGLAP/OCN, bone gamma-carboxyglutamate protein; BL, BL-918; BS, bone surface; BS/TV, bone surface per tissue volume; BV/TV, bone volume per tissue volume; C.C, compound C; CCN1, cellular communication network factor 1; COL1A1, collagen type I alpha 1 chain; COL4A3, collagen type IV alpha 3 chain; COL4A4, collagen type IV alpha 4 chain; COL18A1, collagen type XVIII alpha 1 chain; CQ, chloroquine; GelMA, gelatin methacryloyl hydrogel; GO, Gene Ontology; GSEA, gene set enrichment analysis; HIF1A, hypoxia inducible factor 1 subunit alpha; HPLC, high-performance liquid chromatography; ITGAM/CD11B, integrin subunit alpha M; ITGAX/CD11C, integrin subunit alpha X; ITGB1/CdD9, integrin subunit beta 1; KEGG, Kyoto Encyclopedia of Genes and Genomes; MAP1LC3B/LC3B, microtubule associated protein 1 light chain 3 beta; micro-CT, microcomputed tomography; MSCs, mesenchymal stem cells; MTOR, mechanistic target of rapamycin kinase; NP, negative pressure; NPWT, negative pressure wound therapy; PRKAA1/AMPKα1, protein kinase AMP-activated catalytic subunit alpha 1; PRKAA2, protein kinase AMP-activated catalytic subunit alpha 2; PTPRC/CD45, protein tyrosine phosphatase receptor type C; ROS, reactive oxygen species; RUNX2, RUNX family transcription factor 2; SBI, SBI-0206965; SPP1/OPN, secreted phosphoprotein 1; THY1/CD90, Thy-1 cell surface antigen; SQSTM1, sequestosome 1; TGFB3, transforming growth factor beta 3; ULK1/Atg1, unc-51 like autophagy activating kinase 1. [ABSTRACT FROM AUTHOR]

Simple Summary: Recently, it was found that miR-22 might be involved in the regulation of muscle tenderness and was an important miRNA that affects meat quality. In the present study, we demonstrated that miR-22 regulates the fatty acid composition and mitochondrial morphology of skeletal muscle cells by inhibiting ELOVL6 expression. This study presented the first systematical investigation on the miR-22 correlation with fatty acid composition and mitochondrial function in muscle cells. Our finding is important to help us to understand the function of miR-22 in skeletal muscle cells and the complicacy of muscle metabolism regulation. Recently, miR-22 has been suggested to be an important microRNA (miRNA) affecting meat quality. Studies have shown that muscle fatty acid composition and mitochondrial function are closely related to meat quality. The regulatory mechanism of miR-22 on skeletal muscle fatty acid composition and mitochondrial function is not well characterized. Therefore, we aimed to explore the effects of miR-22 on fatty acid composition and mitochondrial function in C2C12 cells. Here, it demonstrate that elevated expression of miR-22 significantly repressed fatty acid elongation and mitochondrial morphology in C2C12 myoblasts, while the knockdown of miR-22 showed opposite results. Furthermore, miR-22 targets the elongase of very long chain fatty acids 6 (ELOVL6) and represses its expression in muscle cells. Knockdown of ELOVL6 mimicked the effect of miR-22 on fatty acid composition and mitochondrial function, while overexpression of ELOVL6 restored the effects of miR-22. These findings indicate that miR-22 downregulates the elongation of fatty acids and mitochondrial morphology by inhibiting ELOVL6 expression in muscle cells, which may provide some useful information for controlling muscle lipid accumulation and mitochondrial function in livestock in the future. [ABSTRACT FROM AUTHOR]

More and more attention has been paid to the processing of multiword expressions in recent years. This paper reviews empirical studies that have examined the effects of frequency and congruency and their interactive role on the processing of multiword expressions. The results indicated that although frequency and congruency influence the processing of all kinds of multiword expressions, the studies mostly concentrate on collocations; their interactive role with proficiency has not been specified; research exploring the effect of congruency is limited to translational congruency. Future studies can compare the difference in the processing of different kinds of multiword expressions. [ABSTRACT FROM AUTHOR]