Liu, Wenchang, Tan, Hongxin, Luo, Guozhi, Yu, Yongxia, Zhang, Nannan, Yao, Miaolan, Li, Shuang, and Fu, Xianmao
• Extra added carbohydrate with C/NO3--N ratio ≥ 2 at denitrification phase is advisable for nitrogen removal after a nitrification-based BFT aquaculture cycle. • The highest nitrogen recycling rate (23.08 mg-N·g-C-1) could be achieved when the extra added C/NO3–-N ratio was 4.16. • Denitrifying bacteria and denitrifying polyphosphate accumulating organisms were the dominant bacteria during the denitrification process. In the current study, we set up a denitrification process to remove the nitrogen pollutants, especially nitrate (NO 3 –-N), from the wastewater after a nitrification-based biofloc technology (BFT) aquaculture cycle. Five different treatments (CN0, CN1, CN2, CN4 and CN6, respectively) were used, which involved addition of extra carbohydrate with variable ratios of elementary organic carbon to NO 3 –-N by weight (C/NO 3 –-N ratio equal to 0, 1, 2, 4, and 6, respectively). With CN2, CN4, and CN6 treatments, NO 3 –-N was decreased (with increasing alkalinity) to ≤ 6.42 ± 0.30 mg·L−1 and low amounts (close to zero) of nitrite (NO 2 –-N) were achieved. However, there were high concentrations of residual NO 3 –-N and/or NO 2 –-N in CN0 and CN1. CN2 achieved the best denitrification, wherein 81.00 ± 0.95% of the initial input nitrogen was removed. By fitting the equations, the highest nitrogen recycling rate (23.08 mg-N·g-C−1) was achieved with a C/NO 3 –-N ratio of 4.16. Denitrifying bacteria were the dominant bacteria in all extra carbohydrate added treatment groups. Although denitrifying polyphosphate accumulating organisms contributed to the removal of phosphorus, high concentrations of residual soluble reactive phosphate (SRP) were observed in all treatment groups. Overall, extra addition of carbohydrate with C/NO 3 –-N ratio ≥ 2 is advisable for nitrogen removal, while the highest nitrogen recycling rate will be achieved with a ratio of 4.16. [ABSTRACT FROM AUTHOR]