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The objective of this paper is to obtain an excellent structure of a rotational hydrodynamic cavitation reactor (RHCR) by a numerical method and then to investigate the cavitating flow characteristics of the optimized RHCR experimentally. The RNG k-ε turbulence model combined with the Zwart cavitation model was applied to analyze the influence of the straight blade number, the baffle position and the baffle shape on the pressure field, bubble distribution and turbulence kinetic energy of the RHCR. The results show that compared to the original model, an RHCR with a straight blade number of 6, a baffle position of 0.74 and a triangular baffle offers better cavitation performance. Moreover, the energy performance and the cavitation development process of the optimized RHCR were studied experimentally. The results indicate that the multiscale bubbles are induced by straight blades and baffles of the optimized RHCR, accompanied by the twice quasi-periodic shedding dynamics in one cycle. The findings of this study have positive significance for the design and optimization of RHCRs. [ABSTRACT FROM AUTHOR]

The aim of this paper is to introduce a concept of type-2 L-preorders for L being a complete residuated lattice. It can be considered as a common framework of L-preorders and hemimetrics, and also contains various kinds of fuzzy metrics, including Morsi fuzzy metrics, KM-fuzzy metrics and modular metrics, as natural examples. It is shown that the category of L-preordered sets can be reflectively and coreflectively embedded in that of type-2 L-preordered sets. A type-2 L-preorder can be supplied as different models for further study. [ABSTRACT FROM AUTHOR]

Investigation of the breakup and transfer effect of weakly bound nuclei on the fusion process has been an interesting research topic in the past several years. In comparison with radioactive ion beam (RIB), the beam intensities of stable weakly bound nuclei such as 6,7Li and 9Be, which have significant breakup probability, are orders of magnitude higher. Precise fusion measurements induced by these nuclei have already been performed. However, the conclusion of reaction dynamics was not clear and has contradiction. In order to have a proper understanding of the influence of breakup and transfer of weakly bound projectiles on the fusion process, the 6Li+89Y experiment with incident energies of 22 MeV and 34 MeV was performed on Galileo array in combination with Si-ball EUCLIDES at Legnaro National Laboratory (LNL) in Italy. Using the coincidence by the charged particles and γ-rays, the different reaction channels can be clearly identified.

1. Chicken primary myoblasts (CPMs) are precursors that form muscle fibres. The proliferation and differentiation of CPMs is an essential stage in muscle development. Previous RNA-seq analysis showed that phosphoglycerate dehydrogenase (PHGDH) is a differentially expressed gene in chicken muscle tissue at different growth stages. Therefore, the following study explored the effect of PHGDH on the proliferation and differentiation of CPMs. 2. The effect on the proliferation of CPMs by RT–qPCR, CCK-8, and EdU assays after the overexpression and knockdown of PHGDH was evaluated. RT–qPCR, western blotting, and indirect immunofluorescence were used to detect the effect of PHGDH on the differentiation of the CPMs. The expression was observed at different time points for differentiation induced by the CPMs. 3. The results showed that PHGDH significantly promoted proliferation and differentiation in CPMs. The results showed that overexpression of PHGDH significantly upregulated CPM proliferation, while knockdown had the opposite effect. Marker genes showed that overexpression of PHGDH significantly upregulated the expression of P21, MYOG and MYOD genes, significantly downregulated the expression of the MSTN gene and promoted the expression of the MYHC protein. In contrast, PHGDH knockdown had the opposite effect. 4. Desmin immunofluorescence analysis of myotube differentiation in primary myoblasts showed that overexpression of PHGDH significantly increased the area of myotube differentiation and promoted the proliferation and differentiation of myoblasts. Knockdown of PHGDH had the opposite effect. 5. In summary, PHGDH was shown to play a positive role in regulating myoblast proliferation and differentiation. This provides a theoretical basis for further analysis of the regulatory mechanism of the PHGDH gene in chicken muscle development and for improving poultry production. [ABSTRACT FROM AUTHOR]

Investigation of the breakup and transfer effect of weakly bound nuclei on the fusion process has been an interesting research topic in the past several years. However, owing to the low intensities of the presently available radioactive ion beam (RIB), it is difficult to clearly explore the reaction mechanisms of nuclear systems with unstable nuclei. In comparison with RIB, the beam intensities of stable weakly bound nuclei such as 6,7Li and 9Be, which have significant breakup probability, are orders of magnitude higher. Precise fusion measurements have already been performed with those stable weakly bound nuclei, and the effect of breakup of those nuclei on the fusion process has been extensively studied. Those nuclei indicated large production cross sections for particles other than the α + x breakup. The particles are originated from non-capture breakup (NCBU), incomplete fusion (ICF) and transfer processes. However, the conclusion of reaction dynamics was not clear and has the contradiction. In our previous experiments we have performed 6Li+96Zr and 154Sm at HI-13 Tandem accelerator of China Institute of Atomic Energy (CIAE) by using HPGe array. It is shown that there is a small complete fusion (CF) suppression on medium-mass target nucleus 96Zr different from about 35% suppression on heavier target nucleus 154Sm at near-barrier energies. It seems that the CF suppression factor depends on the charge of target nuclei. We also observed one neutron transfer process. However, the experimental data are scarce for medium-mass target nuclei. In order to have a proper understanding of the influence of breakup and transfer of weakly bound projectiles on the fusion process, we performed the 6Li+89Y experiment with incident energies of 22 MeV and 34 MeV on Galileo array in cooperation with Si-ball EUCLIDES at Legnaro National Laboratory (LNL) in Italy. Using particle-particle and particle-γ coincidences, the different reaction mechanisms can be clearly explored.

The fusion excitation function for the positive-Q-value system 12C+30Si (Qfus = +14.1 MeV) has been measured in inverse kinematics down to the μb level and compared with standard coupled-channel calculations. The appearance of the fusion hindrance phenomenon and the evidence of a S-factor maximum have been observed. This result can be significant to extrapolate the behavior of lighter astrophysical relevant systems at deep sub-barrier energies, where existing experimental data are still contradicting and affected by large errors.

1. The goals of this study were to analyse the changes in microbiota composition of chilled chicken during storage and identify microbial biomarkers related to meat freshness. 2. The study used 16S rDNA sequencing to track the microbiota shift in chilled chicken during storage. Associations between microbiota composition and storage time were analysed and microbial biomarkers were identified. 3. The results showed that microbial diversity of chilled chicken decreased with the storage time. A total of 27 and 24 microbial biomarkers were identified by using orthogonal partial least squares (OPLS) and the random forest regression approach, respectively. The receiver operating characteristic (ROC) curve analysis indicated that the OPLS regression approach had better performance in identifying freshness-related biomarkers. The multiple stepwise regression analysis identified four key microbial biomarkers, including Streptococcus, Carnobacterium, Serratia and Photobacterium genera and constructed a predictive model. 4. The study provided microbial biomarkers and a model related to the freshness of chilled chicken. These findings provide a basis for developing detection methods of the freshness of chilled chicken. [ABSTRACT FROM AUTHOR]

Gamma-ray angular distributions in oriented 92 Mo produced via the 6 Li+ 89 Y reaction were studied in this work. The characteristic γ rays in 92 Mo were measured by means of the GALILEO array, which is composed of 25 Compton-suppressed HPGe detectors arranged in a nearly 2 π geometry. A detailed analysis of the spin-alignment attenuation factors ( α 2 ) for different excited states in 92 Mo was performed, by analyzing the measured angular distribution coefficients of several pure-E2 transitions. The relationship between α 2 and the spin was established in 92 Mo and the 244-keV transition between the 5 1 - and 4 1 + states resulted to have a E1 + M2 character with a mixing ratio (δ ) of - 0. 05 - 0.06 + 0.05 , consistent with shell model calculations. [ABSTRACT FROM AUTHOR]

OBJECTIVE: Increasing evidence indicated that N6-methyl-adenosine (M6A) played a key role in a variety of pathophysiological processes. Methylases could promote the processing of mature mi-RNA in a M6A-dependent manner, thereby participating in the pathological cells' occurrence and development. However, the regulatory mechanism of M6A in atherosclerosis (AS) was still unclear. PATIENTS AND METHODS: Quantificational Real-time polymerase chain reaction (qRT-PCR) was used to detect the relative expression levels of M6A, methyltransferase, demethylase transferase, miR-19a and other mi-RNA in atherosclerotic vascular endothelial cells (ASVEC). Cell Counting Kit (CCK8) was used to detect cell proliferation, the expression of PCNA was measured by Western Blot (WB) and qRT-PCR. Transwell assays were used to detect the invasion ability of ASVEC. Co-immunoprecipitation (Co-IP) was used to detect the binding of METTL14 to DGCR8. RNA Immunoprecipitation (RIP) was used to detect the binding of METTL14 to miR-19a. RESULTS: M 6A m odification l evels a nd M ETTL14 methylation transferase were significantly overexpressed in ASVEC. Silencing METTL14 inhibited the proliferation and invasion of ASVEC. Low expression of METTL14 suppressed the binding of methylated RNA and RNA splicing related protein DGCR8. Moreover, silencing METTL14 significantly inhibited the expression of miR-19a while promoted the expression of primary pre-miR-19a. However, high expression of METTL14 obviously increased the expression of DGCR8 and methylated m6A. Furthermore, silencing miR-19a inhibited the proliferation and invasion of ASVEC. CONCLUSIONS: METTL14 increased the M6A modification of pri-miR-19a and promoted the processing of mature miR-19a, thus promoting the proliferation and invasion of ASVEC. These results suggested that METTL14/M6A/miR-19a signaling pathway may be a new target for atherosclerosis treatment. [ABSTRACT FROM AUTHOR]

Rapid population growth and economic development increase energy and grain demands. However, in the high-groundwater coal basins where coal seams and agricultural production overlap, mining subsidence destroys much arable land, causing a series of environmental and social issues. This study investigated plants, beetles, spiders and birds in typical intensive farmlands and farmlands containing mining subsidence mosaics (i.e., mosaic farmlands) in North China. The species composition of these four taxonomic groups differed significantly between the intensive farmlands and mosaic farmlands, with 271 plant, 17 spider, 49 beetle and 138 bird species in the mosaic farmlands and 76, 12, 35 and 30 such species in the intensive farmlands, respectively. The mosaic farmlands hosted more and different species than the intensive farmlands. Additionally, these four taxonomic groups, especially plants and birds, showed a higher abundance and diversity in mosaic farmlands than in intensive ones. These mosaics thus have increased biodiversity conservation value. We emphasize that attention should be paid not only to the environmental damage and human property loss caused by coal mining subsidence but also to the ecological opportunities from the formation of such new habitats. These post-mining habitats represent a new ecological landscape that should be a part of natural conservation. [ABSTRACT FROM AUTHOR]

OBJECTIVE: This study aimed to explore the expression of JMJD3 in non-small cell lung cancer (NSCLC) and to further study its association with clinical features and prognosis of patients. PATIENTS AND METHODS: Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was performed to examine the level of JMJD3 in 46 pairs of NSCLC tissues and para-cancerous specimens. The relationship between JMJD3 level and clinical features of NSCLC and patients’ prognosis was analyzed. And JMJD3 expression in NSCLC cells was further verified by qRT-PCR. In addition, JMJD3 knockdown model was constructed using siRNA in cell lines including A549 and SPC-A1, and the effect of JMJD3 on the biological function of NSCLC cells was analyzed by cell counting kit-8 (CCK-8) and transwell migration and invasive-ness assays. Lastly, Western blot was performed to explore the potential mechanism. RESULTS: In this investigation, qRT-PCR results indicated that JMJD3 expression in above-mentioned tumor tissues was conspicuously higher than that in normal tissues. In addition, compared with patients with low level of JMJD3, patients with high level of JMJD3 had a higher incidence of lymphatic metastasis and distant metastasis and a lower overall survival rate. Meanwhile, the proliferation, invasiveness and migratory capacity of cells in the sh-JMJD3 group was conspicuously decreased when compared with the cells in negative control group. Western Blot results indicated that the levels of key proteins in EMT signaling pathway such as E-cadherin, N-cadherin, Vimentin, TGF-β, and MMP-9 were notably decreased in sh-JMJD3 group. Besides, the addition of TGF-β cytokines synergistically promoted the malignant progression of NSCLC induced by JMJD3. CONCLUSIONS: JMJD3 expression was found conspicuously increased in NSCLC, which might be close relevant to NSCLC lymphatic or distant metastasis as well as patients’ poor prognosis. Therefore, we speculated that JMJD3 could promote invasiveness and migratory capacity of non-small cell lung cancer cells by activating EMT process. [ABSTRACT FROM AUTHOR]