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Background: Poor reporting quality and spin in randomized controlled trial (RCT) abstracts can lead to misinterpretation and distorted interpretation of results. Objectives: This methodological study aimed to assess the reporting quality and spin among RCT abstracts on splint therapy for temporomandibular disorders (TMD) and explore the association between spin and potentially related factors. Methods: The authors searched PubMed for RCTs on splint therapy for TMD. The reporting quality of each abstract was assessed using the original 16‐item CONSORT for abstracts checklist. The authors evaluated the presence and characteristics of spin only in abstracts with nonsignificant primary outcomes according to pre‐determined spin strategies. Logistic regression analyses were performed to identify factors associated with the presence of spin. Results: A total of 148 abstracts were included in the reporting quality evaluation. The mean overall CONSORT score (OCS) was 5.86 (score range: 0–16). Only interventions, objectives and conclusions were adequately reported. Of the 61 RCT abstracts included for spin analysis, spin was identified in 38 abstracts (62.3%), among which 32 abstracts (52.3%) had spin in the Results section and 21 (34.4%) had spin in the Conclusions section. A significantly lower presence of spin was found in studies with exact p‐value reporting (OR: 0.170; 95% CI: 0.032–0.887; p =.036) and a two‐arm comparison design (OR: 11.777; 95% CI: 2.171–63.877; p =.004). Conclusions: The reporting quality of RCT abstracts on splint therapy for TMD is suboptimal and the prevalence of spin is high. More awareness and joint efforts are needed to improve reporting quality and minimize spin. [ABSTRACT FROM AUTHOR]

Objective: The association between jaw function and income in patients with temporomandibular disorders (TMDs) remain unclear. The aim of this study was to explore this association and its relationship with anxiety and depression. Subjects and Methods: A total of 451 TMD patients, including 361 males and 90 females, participated in this study. The sociodemographic information of patients and their questionnaires including the Generalised Anxiety Disorder 7‐item (GAD‐7), Patient Health Questionnaire 9‐item (PHQ‐9), and Jaw Functional Limitation Scale‐8 (JFLS‐8) were collected. Patients were divided into the high‐income and low‐income groups based on a household per capita income of 6000 RMB per month. Multiple regression and mediation analysis were used to explore the association between variables. The bootstrap method was applied to estimate confidence intervals (CIs). Results: Higher JFLS‐8 scores were significantly correlated with higher GAD‐7 scores (r = 0.361, p <.001), PHQ‐9 scores (r = 0.339, p <.001). Females and patients with low income had statistically higher JFLS‐8 scores (p <.01, p <.001). Mediation analysis with 10 000 bootstrap simulations revealed a significant direct association between JFLS‐8 scores and income (−2.920, 95% CI [−4.757, −1.044], p =.002). A significant indirect association of JFLS‐8 scores with income via GAD‐7 scores and PHQ‐9 scores was also observed (−0.889, 95% CI [−1.728, −0.164], p =.025), accounting for 23.3% of the total association. Conclusions: Low income is associated with impaired jaw function via anxiety and depression in patients with TMD. Clinicians may need to pay more attention to the psychological status of low‐income TMD patients in clinical practice. [ABSTRACT FROM AUTHOR]

Rhodamine dyes, as star molecules of fluorescent dyes, are critical tools in modern analytical and biochemical research. However, switching their chiroptical properties still remains a big challenge because of the absence of a suitable synthesis method to incorporate through conjugation a chiral fragment into the chromophore core of rhodamine. Herein, a simple and straightforward way is utilized to link two rhodamine chromophores by different chiral bridges. The conjugated RRR/SSS birhodamine dyes with one axial chirality, which is constructed directly by two rhodamine chromophores, and two spirocyclic chiralities show the interesting circular dichroism and circularly polarized luminescence switching and lysosome‐targetable properties. On the other hand, the unconjugated RR/SS birhodamine dyes with two chiral carbons do not have such chiroptical switching properties but do have different amazing cellular staining properties. The RR birhodamine dye can stain both lysosomes and mitochondria, but SS birhodamine dye can stain lysosomes only. Moreover, the mechanisms of chiroptical switching and diastereoselectivity are investigated as well, based on single‐crystal structures and density functional theory calculations. Therefore, a new platform is afforded here in designing chiral dyes for chiroptical switching and cell imaging applications. [ABSTRACT FROM AUTHOR]

Purpose: This study aims to explore the association of pressure pain thresholds (PPTs) with age, gender, and pain in patients with temporomandibular disorders (TMD). Patients and Methods: A total of 301 TMD patients (248 female and 53 male) were recruited and classified into the high and low age groups according to their median age of 26 years. Patients' demographics, pain-related variables, TMD-related variables, and PPTs of both left and right temporomandibular joints (TMJs), masseter, and temporalis were collected. Results: Pain duration and visual analog scale of pain (VAS) showed no significant correlations with PPTs (P 0.05). Multiple linear regression analysis revealed a significant positive association of PPTs of all six sites with males (β=0.41– 0.72 kg·cm− 2, 95% CI (0.19– 0.38, 0.74– 0.99), P< 0.001), as well as with the high age group [β=0.28– 0.36 kg·cm− 2, 95% CI (0.07– 0.20, 0.47– 0.53), P< 0.020]. Furthermore, PPTs of the left TMJ showed a significant negative association with left pain-related TMD (PT) [β=− 0.21 kg·cm− 2, 95% CI (− 0.38, − 0.04), P=0.026], but PPTs of the remaining sites did not show a significant association with PT (P 0.05). Stratified analysis showed that PPTs in females were associated with the high age group [β=0.25– 0.37 kg·cm− 2, 95% CI (0.04– 0.20, 0.45– 0.56), P< 0.020] and that PPT of the left TMJ was associated with left PT [β=− 0.21 kg·cm− 2, 95% CI (− 0.39, − 0.03), P=0.043]. The remaining PPTs did not show a significant association with PT (P 0.05). In males, PPTs did not show significant correlations with age, PT and VAS (P 0.05). Conclusion: PPTs in the orofacial region are associated with gender and age in TMD patients. Pain duration and intensity show no significant correlations with PPTs in TMD patients. Researchers and dentists should take age and gender into account when using PPTs as auxiliary diagnostic indicators for PT. [ABSTRACT FROM AUTHOR]

Periodontitis is one of the most prevalent infectious inflammatory diseases, characterized by irreversible destruction of the supporting tissues of teeth, which is correlated with a greater risk of multiple systemic diseases, thus regarded as a major health concern. Dysregulation between periodontal microbial community and host immunity is considered to be the leading cause of periodontitis. Comprehensive studies have unveiled the double‐edged role of immune response in the development of periodontitis. Immune senescence, which is described as age‐related alterations in immune system, including a diminished immune response to endogenous and exogenous stimuli, a decline in the efficiency of immune protection, and even failure in immunity build‐up after vaccination, leads to the increased susceptibility to infection. Recently, the intimate relationship between immune senescence and periodontitis has come into focus, especially in the aging population. In this review, both periodontal immunity and immune senescence will be fully introduced, especially their roles in the pathology and progression of periodontitis. Furthermore, novel immunotherapies targeting immune senescence are presented to provide potential targets for research and clinical intervention in the future. [ABSTRACT FROM AUTHOR]

Background: Temporomandibular joint osteoarthritis (TMJ‐OA) causes severe symptoms such as chewing difficulties, acute pain and even maxillofacial deformity. However, there is hardly any effective disease‐curing strategy because of uncertainty in aetiology. Animal model is an excellent tool to investigate the mechanism, prevention and treatment on diseases. Currently, although several TMJ‐OA animal models have been established, there are almost no comparative studies on different models, which poses a great challenge for selecting suitable models. Objective: To compare three TMJ‐OA induction methods and assess their applicability considering pathological changes in the cartilage, subchondral bone, osteoclasts, and synovium. Methods: Murine models were employed and followed for 3 and 6 weeks after experimental procedures (surgery, injection, crossbite). The TMJ changes were evaluated by Safranin‐O/Fast green staining, immunofluorescence staining, micro‐CT, TRAP staining, and HE staining. Results: In the Surgery group, a pronounced drop in bone volume fraction was observed. In the Injection group, chondrocytes were mostly disordered or arranged in clusters and a substantial increase in the OARSI score and osteoclasts was found. The OARSI score and osteoclasts also increased significantly in the Crossbite group, although to a lower extent compared with injection. Conclusion: Osteoarthritis‐like changes were observed in all models. Concerning the applicability of the different induction methods, surgery might be an important resource for the assessment of post‐traumatic TMJ‐OA and subchondral bone changes in early stages. Injection induces a severe end‐stage osteoarthritis in a short time and provides model basis for advanced TMJ‐OA. Crossbite might be more reasonable model to explore the pathogenesis mechanism of temporomandibular arthritis due to occlusal disorders. [ABSTRACT FROM AUTHOR]

Inflammatory osteolysis is usually linked to the activation of proinflammatory macrophage and the consequent excessive osteoclast formation. Emerging evidence indicates that agents or drugs targeting lipid metabolism in macrophages might be potential in the prevention and treatment of osteolysis. d -mannose, as a natural-existed metabolic regulator, exerts strong effects on attenuating osteopenia and inflammation. However, whether d -mannose is therapeutically effective on osteolysis and whether a metabolic mechanism counts for the effect remain to be addressed. Here, by using an in vivo lipopolysaccharide (LPS)-induced inflammatory osteolysis mouse model as well as an in vitro LPS-induced inflammatory macrophage culture system, we show that d -mannose attenuates inflammatory osteolysis and inhibits excessive osteoclastogenesis by reversing the LPS-induced activation of proinflammatory macrophage. Mechanically, d -mannose recovers LPS-suppressed Cpt1a transcription and promotes lipid metabolism of macrophage. Treatment with etomoxir, an inhibitor of CPT1A, abolishes the effects of d -mannose on LPS-treated macrophage in vitro and eliminates its protection against osteolysis in vivo. Collectively, our results imply that d -mannose attenuates LPS-induced osteolysis by manipulating CPT1A-mediated lipid metabolism in macrophages. Our results disclose the unrecognized utilization of d -mannose as an effective intervention against inflammatory osteolysis and provide evidence to manage inflammatory scenarios by therapeutically targeting lipid metabolism in macrophage. [Display omitted] • d -Mannose effectively alleviates LPS-induced calvarial osteolysis and inhibits excessive osteoclastogenesis. • d -mannose reverses the LPS-induced macrophage switch to proinflammatory (M1) subtype. • d -mannose suppresses excess lipid storage in inflammatory macrophages. • CPT1A-manipulated lipid metabolism in macrophage mediates the role of d -mannose in osteolysis. [ABSTRACT FROM AUTHOR]

This document is a corrigendum for an article titled "Age and Gender, but Not Pain are Associated with Pressure Pain Thresholds in Patients with Temporomandibular Disorders: A Cross-Sectional Study." The authors acknowledge that they failed to include a funding statement in the published paper and provide the correct statement in this document. The funding for the study was provided by the Technology Innovation Project of Science and Technology Bureau of Chengdu and the Clinical Research Project of West China Hospital of Stomatology, Sichuan University. The authors apologize for the oversight. [Extracted from the article]

Objectives: Chondrocyte ferroptosis contributes to osteoarthritis (OA) progression, and D‐mannose shows therapeutic value in many inflammatory conditions. Here, we investigated whether D‐mannose interferes in chondrocyte ferroptotic cell death during osteoarthritic cartilage degeneration. Materials and methods: In vivo anterior cruciate ligament transection (ACLT)‐induced OA mouse model and an in vitro study of chondrocytes in an OA microenvironment induced by interleukin‐1β (IL‐1β) exposure were employed. Combined with Epas1 gene gain‐ and loss‐of‐function, histology, immunofluorescence, quantitative RT‐PCR, Western blot, cell viability and flow cytometry experiments were performed to evaluate the chondroprotective effects of D‐mannose in OA progression and the role of hypoxia‐inducible factor 2 alpha (HIF‐2 α) in D‐mannose‐induced ferroptosis resistance of chondrocytes. Results: D‐mannose exerted a chondroprotective effect by attenuating the sensitivity of chondrocytes to ferroptosis and alleviated OA progression. HIF‐2α was identified as a central mediator in D‐mannose‐induced ferroptosis resistance of chondrocytes. Furthermore, overexpression of HIF‐2α in chondrocytes by Ad‐Epas1 intra‐articular injection abolished the chondroprotective effect of D‐mannose during OA progression and eliminated the role of D‐mannose as a ferroptosis suppressor. Conclusions: D‐mannose alleviates osteoarthritis progression by suppressing HIF‐2α‐mediated chondrocyte sensitivity to ferroptosis, indicating D‐mannose to be a potential therapeutic strategy for ferroptosis‐related diseases. [ABSTRACT FROM AUTHOR]

The underlying mechanisms and treatment of painful temporomandibular disorders (TMDs) are important but understudied topics in craniofacial research. As a group of musculoskeletal diseases, the onset of painful TMD is proved to be a result of disturbance of multiple systems. Recently, emerging evidence has revealed the involvement of neuroimmune interactions in painful TMD. Inflammatory factors play an important role in peripheral sensitization of temporomandibular joint (TMJ), and neurogenic inflammation in turn enhances TMJs dysfunction in TMD. Furthermore, centralized neuroimmune communications contribute to neuron excitability amplification, leading to pain sensitization, and is also responsible for chronic TMD pain and other CNS symptoms. Therapeutics targeting neuroimmune interactions may shed light on new approaches for treating TMD. In this review, we will discuss the role of neuroimmune interactions in the onset of painful TMD from the peripheral and centralized perspectives, and how understanding this mechanism could provide new treatment options. Insights into the neuroimmune interactions within TMJs and painful TMD would broaden the knowledge of mechanisms and treatments of this multifactorial disease. [ABSTRACT FROM AUTHOR]

Bone stromal cells are critical for bone homeostasis and regeneration. Growing evidence suggests that non-stem bone niche cells support bone homeostasis and regeneration via paracrine mechanisms, which remain to be elucidated. Here, we show that physiologically quiescent SM22α- lineage stromal cells expand after bone injury to regulate diverse processes of intramembranous bone regeneration. The majority of SM22α- lineage cells neither act as stem cells in vivo nor show their expression patterns. Dysfunction of SM22α- lineage niche cells induced by loss of platelet-derived growth factor receptor β (PDGFRβ) impairs bone repair. We further show that PDGFRβ-triggered hydrogen sulfide (H 2 S) generation in SM22α- lineage niche cells facilitates osteogenesis and angiogenesis and suppresses overactive osteoclastogenesis. Collectively, these data demonstrate that non-stem SM22α- lineage niche cells support the niche for bone regeneration with a PDGFRβ/H 2 S-dependent regulatory mechanism. Our findings provide further insight into non-stem bone stromal niche cell populations and niche-regulation strategy for bone repair. [Display omitted] • Non-stem SM22α- lineage niche cells are activated by injury to drive bone regeneration • Loss of PDGFRβ induces dysfunction of SM22α- lineage cells and then impairs bone repair • PDGFRβ, but not PDGFRα, is required for H 2 S production of SM22α- lineage niche cells • PDGFRβ-driven H 2 S production by SM22α- lineage cells regulates stem and progenitor cell fate Zhou et al. identify the distinct function of SM22α- lineage stromal cells in regulating diverse processes of intramembranous bone regeneration. These niche cells are activated upon injury and modulate stem and progenitor cell fate with a unique PDGFRβ/H 2 S-dependent regulatory mechanism. [ABSTRACT FROM AUTHOR]

Objectives Materials and Methods Results Conclusion To investigate the prevalence and associated factors of health anxiety (HA) in patients with Temporomandibular Disorders (TMDs) using the 8‐item Whiteley Index (WI‐8) scale.Three hundred and twenty‐nine TMDs patients completed the Visual Analog Scale (VAS), WI‐8, Jaw Functional Limitation Scale‐8 (JFLS‐8), Patient Health Questionnaire‐9 (PHQ‐9), and Generalized Anxiety Disorder‐7 (GAD‐7) scales. Clinical examinations were conducted following the Diagnostic Criteria for TMDs Axis I.The prevalence of HA among TMDs patients was 18.54%. Patients with HA had higher scores of VAS‐current (p = 0.026), VAS‐maximum (p = 0.024), VAS‐average (p = 0.030), JFLS‐8 (p < 0.001), GAD‐7 (p < 0.001) and PHQ‐9 (p < 0.001), lower maximum mouth opening (p = 0.016), lower proportion of structure‐related TMDs (p = 0.028), and higher proportion of pain‐related TMDs (p < 0.001) compared to those without HA. The correlation coefficient was 0.61 (p < 0.001) between WI‐8 and GAD‐7 and 0.64 (p < 0.001) between WI‐8 and PHQ‐9.Approximately one‐fifth of patients with TMDs experienced HA. HA was associated with pain perception, functional limitations, depressive, and anxiety symptoms in individuals with TMDs. HA may contribute to heightened subjective pain experiences rather than structural changes in the TMJ. [ABSTRACT FROM AUTHOR]

Scope: Periodontitis is one of the most prevalent chronic inflammatory diseases with impaired autophagy. Agaricus blazei Murrill polysaccharide (ABMP) shows beneficial effects in various inflammatory diseases. However, whether ABMP is involved in autophagy regulation and periodontitis attenuation remains to be elucidated., Methods and Results: This study firstly shows the dynamic changes in inflammatory and autophagy levels in silk ligature periodontitis model. Then the positive regulation effect of autophagy on inflammation and its vital role in ABMP inhibiting PDLCs inflammatory response are testified in LPS-treated PDLCs. Secondly, the Micro-CT, quantitative RT-PCR, Western Blot, TRAP, and immunofluorescence staining analysis are performed to assess the effects of ABMP on periodontitis and autophagy. The data show the augmented autophagy and alleviated gingival recession, inflammatory cell infiltration, alveolar bone resorption, and reduced osteoclasts in periodontitis by ABMP treatment. Further experiments using chemical inhibitors demonstrate the vital role of H 2 S/NRF2 axis in ABMP-induced appropriate level of autophagy augmentation against periodontitis., Conclusions: Collectively, the findings not only reveal the unrecognized capacity and mechanism of ABMP as an effective and potential dietary intake against periodontitis, but also suggest the possibility for ABMP to be used in the treatment of other autophagy-related diseases., (© 2023 Wiley-VCH GmbH.)

Temporomandibular joint osteoarthritis (TMJOA) is a debilitating degenerative disease with high incidence, deteriorating quality of patient life. Currently, due to ambiguous etiology, the traditional clinical strategies of TMJOA emphasize on symptomatic treatments such as pain relief and inflammation alleviation, which are unable to halt or reverse the destruction of cartilage or subchondral bone. A number of studies have suggested the potential application prospect of mesenchymal stem cells (MSCs)-based therapy in TMJOA and other cartilage injury. Worthy of note, exosomes are increasingly being considered the principal efficacious agent of MSC secretions for TMJOA management. The extensive study of exosomes (derived from MSCs, synoviocytes, chondrocytes or adipose tissue et al.) on arthritis recently, has indicated exosomes and their specific miRNA components to be potential therapeutic agents for TMJOA. In this review, we aim to systematically summarize therapeutic properties and underlying mechanisms of MSCs and exosomes from different sources in TMJOA, also analyze and discuss the approaches to optimization, challenges, and prospects of exosome-based therapeutic strategy., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Yuan, Wu, Huang, Zhou, Liu, Yi, Wang and Liu.)

Objective: To provide reliable prediction models based on dentoskeletal and soft tissue variables for customizing maxillary incisor positions and to optimize digitalized orthodontic treatment planning., Methods: This study included 244 Chinese women (age, 18-40 years old) with esthetic profiles after orthodontic treatment with fixed appliances (133 in group I: 1° ≤ The angle between the nasion [N]-A point [A] plane and the N-B point [B] plane [ANB] ≤ 4°; 111 in group II: 4° < ANB ≤ 7°). Dental, skeletal, and soft tissue measurements were performed on lateral cephalograms of the participants. Correlation and multiple linear regression analyses were used to determine the influence of dentoskeletal and soft tissue variables on maxillary incisor position., Results: The ideal anteroposterior position of the maxillary incisor varied between sagittal skeletal patterns. The position of the maxillary incisor correlated with the sagittal discrepancy between the maxilla and the mandible (ANB), protrusion of the midface, nasal tip projection, development of the chin, and inclination of both the maxillary and mandibular incisors. Distance from the maxillary central incisor to nasion-pogonion plane predicted using multiple linear regression analysis was accurate and could be a practical measurement in orthodontic treatment planning., Conclusions: Instead of using an average value or norm, orthodontists should customize a patient's ideal maxillary incisor position using dentoskeletal and soft tissue evaluations.

Mesenchymal stem cells (MSCs) are remarkable and noteworthy. Identification of markers for MSCs enables the study of their niche in vivo. It has been identified that glioma-associated oncogene 1 positive (Gli1+) cells are mesenchymal stem cells supporting homeostasis and injury repair, especially in the skeletal system and teeth. This review outlines the role of Gli1+ cells as MSC subpopulation in both bones and teeth, suggesting the prospects of Gli1 an + cells in stem cell- based tissue engineering., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)

Osteoporosis is a prevalent bone disorder characterized by bone mass reduction and deterioration of bone microarchitecture leading to bone fragility and fracture risk. In recent decades, knowledge regarding the etiological mechanisms emphasizes that inflammation, oxidative stress and senescence of bone cells contribute to the development of osteoporosis. Studies have demonstrated that heme oxygenase 1 (HO-1), an inducible enzyme catalyzing heme degradation, exhibits anti-inflammatory, anti-oxidative stress and anti-apoptosis properties. Emerging evidence has revealed that HO-1 is critical in the maintenance of bone homeostasis, making HO-1 a potential target for osteoporosis treatment. In this Review, we aim to provide an introduction to current knowledge of HO-1 biology and its regulation, focusing specifically on its roles in bone homeostasis and osteoporosis. We also examine the potential of HO-1-based pharmacological therapeutics for osteoporosis and issues faced during clinical translation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Zhou, Yuan, Xiong, Zhang, Liu, Zheng, Wang and Liu.)

In the present work, a chemical reaction has been performed for supramolecular gels to achieve multiple-stimuli-responsive smart soft materials. Simple chiral binaphthalene-based receptors can condense with histidine (His) to yield a Schiff base, which would help to achieve visual chiral recognition of unprotected l / d -His through gel formation along with specific selectivity toward 20 amino acids. Through intermolecular hydrogen bonds, the resultant Schiff base molecules assemble with excess His molecules to form three-dimensional (3D) networks of metastable cross-linked nanospheres and stable nanofibers in EtOH/water and MeOH/water, respectively. Significantly, this condensation reaction exhibits unique reversible and chromatic phenomena between sol-gel phase transitions in EtOH/water, which provide a way to design chemical reaction-based multivisual-change supramolecular gels for sensing and switching applications.