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Background and Objective: Vocal changes are one of the most common consequences of thyroid surgery. These changes can be due to neurological or other factors. With respect to vocal changes after surgery, and the need for determination of details of the vocal problems and related consequences in the patients’ life after thyroidectomy, the aim of this study was determination of some acoustic parameters of their voice after thyroidectomy and the status of subscales of Voice Handicap Index (VHI). Methods: This study is a cross-sectional descriptive analytic and non-interventional. One voice of 32 thyroidectomized patients (23 women and 9 men) with 20-60 years of age analyzed acoustically and they completed VHI questionnaire. Results: Statistical comparison of mean scores of VHI in two groups of patients with and without vocal problem showed that mean score and standard deviation of total scores of VHI, and the scores of functional, emotional, and physical subscales in patients with voice problems are more than the other group, and the differences were significant (P=0.000). Comparison of acoustic parameters in the two groups of patients showed that F0, jitter, shimmer, and HNR were different, but the differences were not significant. According to our results, amplitude perturbations and harmonic to noise ratio was high in both groups of thyroidectomized patients. Conclusion: According to the findings of this research, we can conclude that F0, jitter, shimmer, and HNR of voice may be not enough for determination of voice problems, but other acoustic measures, self-perceptual, or self-reported evaluation tools such as VHI maybe more efficient in determination of thyroidectomized patients’ voice problems.