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12. LIST OF CONTRIBUTORS

Author

Abu-Shakra, Mahmoud, primary, Achiron, Anat, additional, Alderuccio, Frank, additional, Amital, Howard, additional, Ansari, Aftab A., additional, Aoki, Christopher A., additional, Arepally, Gowthami, additional, Aries, Peer M., additional, Atzeni, Fabiola, additional, Bachmann, Michael, additional, Bendtzen, Klaus, additional, Berden, Jo H., additional, Bertolaccini, Maria Laura, additional, Betterle, Corrado, additional, Bianchi, Francesco B., additional, Binder, Steven R., additional, Bizzaro, Nicola, additional, Borghi, Maria O., additional, Bowlus, Christopher L., additional, Božič, Borut, additional, Bradwell, Arthur R., additional, Bruschi, Maurizio, additional, Burek, C. Lynne, additional, Candiano, Giovanni, additional, Carp, H.J.A., additional, Caturegli, Patrizio, additional, Cerinic, Marco Matucci, additional, Cervera, Ricard, additional, Chan, Edward K.L., additional, Chang, Yih-Hsin, additional, Chen, Shu, additional, Cicardi, Marco, additional, Cines, Douglas B., additional, Cohen, Irun R., additional, Conrad, Karsten, additional, Csernok, Elena, additional, Čučnik, Saša, additional, De Angelis, Valentina, additional, Dier, Ken J., additional, Dotan, Nir, additional, Dueymes, Maryvonne, additional, Dumortier, Hélène, additional, Lahawi, Mohammed El, additional, Faulk, W. Page, additional, Feist, Eugen, additional, Forster, Olaf, additional, Fritzler, Marvin J., additional, Frostegård, Johan, additional, Fujinami, Robert S., additional, Furmaniak, Jadwiga, additional, Galli, Monica, additional, Gerosa, Maria, additional, Gershwin, M. Eric, additional, Ghiggeri, Gian Marco, additional, Gilles, Jean Guy, additional, Grando, Sergei A., additional, Graus, Francesc, additional, Gross, Wolfgang L., additional, Guiducci, Serena, additional, Guilpain, Philippe, additional, Hamann, Dörte, additional, Hamilton, Robert G., additional, Harel, Michal, additional, Haung, Ming-Lih, additional, Heidenreich, Fedor, additional, Hellmark, Thomas, additional, Herkel, Johannes I., additional, Hershko, Alon Y., additional, Hiepe, Falk, additional, Hilfiker-Kleiner, Denise, additional, Hughes, Richard G., additional, Hultman, Per, additional, Invernizzi, Pietro, additional, Izui, Shozo, additional, Jirawuthiworavong, Guy V., additional, Kallenberg, Cees G.M., additional, Karim, Abid R., additional, Kaveri, Srinivas V., additional, Khamashta, Munther A., additional, Kind, Michaela, additional, Knoflach, Michael, additional, Kodermaz, Graziano, additional, Krueger, Christian, additional, Kveder, Tanja, additional, Landolfo, Santo, additional, Lang, Bethan, additional, Leung, Patrick S.C., additional, Levy-Clarke, Grace A., additional, Lindert, Ralf-Björn, additional, Lochman, Ivo, additional, Lochmanová, Alexandra, additional, Lopez, Luis R., additional, Loy, Anthony Chin, additional, Manns, Michael P., additional, Matsumoto, Isao, additional, Matsuura, Eiji, additional, McCarty, Gale A., additional, McHugh, Neil John, additional, McIntyre, John A., additional, Meroni, Pier Luigi, additional, Miron, Shmuel, additional, Mondini, Michele, additional, Mouthon, Luc, additional, Muller, Sylviane, additional, Muratori, Paolo, additional, Musante, Luca, additional, Muthugounder, Sakunthala, additional, Naguwa, Stanley, additional, Nakamura, Tomomi, additional, Naparstek, Yaakov, additional, Narain, Sonali, additional, Nussenblat, Robert B., additional, Nydegger, Urs E., additional, Oertelt, Sabine, additional, Pengo, Vittorio, additional, Peterson, Lisa K., additional, Pietropaolo, Massimo, additional, Podda, Mauro, additional, Pollard, K. Michael, additional, Poncz, Mortimer, additional, Prestigiacomo, Tony, additional, Radice, Antonella, additional, Randone, Silvia Bellando, additional, Raschi, Elena, additional, Ravindranath, Mepur H., additional, Ravindranath, Rajeswari M.H., additional, Reeves, Westley H., additional, Reichlin, Morris, additional, Renaudineau, Yves, additional, Riboldi, Piersandro, additional, Ronda, Nicoletta, additional, Rose, Noel R., additional, Rosenberg, Nurit, additional, Routsias, John G., additional, Rozman, B., additional, Ruffatti, Amelia, additional, Sabadini, Ettore, additional, Saint-Remy, Jean-Marie, additional, Salomon, Ophira, additional, Santucci, Laura, additional, Sarzi-Puttini, Piercarlo, additional, Satoh, Minoru, additional, Schlosser, Michael, additional, Scofield, R. Hal, additional, Segelmark, Mårten, additional, Selmi, Carlo, additional, Seo, Suk, additional, Shenkman, Boris, additional, Shiau, Ming-Yuh, additional, Shoenfeld, Yehuda, additional, Sinico, Renato Alberto, additional, Sliwa, Karen, additional, Smeenk, Ruud J.T., additional, Smolen, Josef S., additional, Sperling, Mark A., additional, Stadler, Beda M., additional, Steiner, Günter, additional, Stöcker, Winfried, additional, Strassburg, Christian P., additional, Sumida, Takayuki, additional, Sundstrom, J. Bruce, additional, Svenson, Morten, additional, Targoff, Ira N., additional, Tedesco, Francesco, additional, Toh, Ban-Hock, additional, Tonutti, Elio, additional, Torens, Isabel, additional, Torok, Natalie, additional, Tozzoli, Renato, additional, Tsokos, George C., additional, Tzioufas, Athanasios G., additional, Ulmansky, Rina, additional, van Bavel, Casandra C., additional, van der Vlag, Johan, additional, Villalta, Danilo, additional, Vincent, Angela, additional, Wagenknecht, Dawn R., additional, Wakamatsu, Ei, additional, Wener, Mark H., additional, Wesierska-Gadek, Józefa, additional, Wick, Georg, additional, Wiik, Allan, additional, Willison, Hugh J., additional, Witte, Torsten, additional, Wurster, Ulrich, additional, Youinou, Pierre, additional, Zanchetta, Renato, additional, Zandman-Goddard, Gisele, additional, Zhuang, Haoyang, additional, Zingale, Lorenza C., additional, and Zuliani, Luigi, additional

Published
2007
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16. Consequences of Opiate-Dependency in a Monkey Model of AIDS

Author

Donahoe, Robert M., primary, Byrd, Larry D., additional, McClure, Harold M., additional, Fultz, Patricia, additional, Brantley, Mary, additional, Marsteller, Frederick, additional, Ansari, Aftab Ahmed, additional, Wenzel, DeLoris, additional, and Aceto, Mario, additional

Published
1993
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24. Immunological Tolerance in Allo- and Xenografts.

Author

Gershwin, M. Eric, Vierling, John M., Manns, Michael P., Ansari, Aftab A., and Pattanapanyasat, Kovit

Abstract

Advances in surgical techniques and ancillary care, in parallel with advances in clinical immunosuppression, are the reasons for the enormous success that has been achieved to date in solid organ transplantation. Such success has also provided a considerable boost in the advancement of our knowledge of immunological tolerance in allogeneic and (relatively more recently) xenogeneic transplantation. Thus, success in human organ transplantation has provided the incentive and foundation for unraveling some of the mysteries of organ transplant acceptance vs failure and has been the foundation for the science of transplantation immunology, which includes to a large extent studies of the fundamental mechanisms of immunological tolerance and the understanding of the concepts involved in self- vs nonself-discrimination. [ABSTRACT FROM AUTHOR]

Published
2007
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26. Cytokine Analysis by Intracellular Staining.

Author

Walker, John M., Doolan, Denise L., Ansari, Aftab A., and Mayne, Ann E.

Abstract

The advent of molecular techniques for the first time allowed for the specific biological characterization and a more clear understanding of the function of molecules synthesized by leukocytes termed cytokines. In addition, availability of the recombinant forms of these cytokines with defined sequences and in select cases molecular structure allowed for a rationale to group the growing list of cytokines and their cognate receptors. Cytokines in general are proteins or glycoproteins that are synthesized by a number of cell lineages, the best characterized are those synthesized by the hematopoietic cell lineages. Cytokines in general are either secreted, expressed on the cell membrane and/or held in a reservoir form within the cells that synthesize them. Several cytokines are synthesized in a biologically inactive form (procytokine) and need to be cleaved to release the active form, a notable example being tumor necrosis factor-α (TNF-α). Certain cytokines are held as storage depots within tissue areas within which their function is manifest. Thus cytokines that perform hematopoietic growth and differentiation appear to be stored as depots within stromal cell layers of the bone marrow, whereas those involved in tissue injury and repair are localized to the extracellular matrix, skin and bone and so on. Most, if not all, induce their biological effect by binding to their cognate receptors, which provide intracellular activation of signal transduction and second-messenger pathways. Most cytokines also appear to serve as growth factors or differentiation-inducing molecules for hematopoietic cell lineages. [ABSTRACT FROM AUTHOR]

Published
2002
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27. Tailed RT-PCR for the Quantitation of Chloramphenicol Acetyl Transferase (CAT)mRNA.

Author

Walker, John M., Kochanowski, Bernd, Reischl, Udo, Knuchel, Marlyse C., and Ansari, Aftab A.

Abstract

Reporter gene plasmids have been used extensively to monitor gene expression and elucidate intracellular pathways (1-4). They have been particularly useful in understanding the architecture of promoter regions and the interactions between promoter elements and cellular or viral regulatory factors (5-9). The conventional strategy has been to transfect host cells transiently with a plasmid bearing the sequences of interest linked to a chloramphenicol acetyl transferase (CAT) reporter gene. Subsequently, CAT activity is measured as a readout by thin-layer chromatography (TLC) or the levels of CAT protein are determined using an enzyme-linked immunosorbent assay (ELISA). However, most transfections-whether stable or transient-result in low levels of CAT gene expression, as long as no activation signal is provided (10-12). Although this is an ideal situation to study gene activation pathways, it is poorly suited to monitor gene repression or negative regulatory mechanisms. To overcome this problem, investigators use cell-activating agents, such as phorbolester and phytohemagglutinin, or transfect a second plasmid expressing a transactivator (e.g., viral transactivator). However, use of such agents might interfere with the pathway(s) being studied and could provide erroneous results. Also, current assays such as TLC or CAT ELISA are often not able to quantitate such low levels of expression, thus hampering studies aimed at dissecting the down modulation of gene expression (13-15). [ABSTRACT FROM AUTHOR]

Published
1999
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