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On occasion, the pathologist may have insufficient tissue to make a diagnosis, the tissue is altered or damaged in processing, or lesions may have overlapping features, but the lesion was not sampled in a way to make a definitive diagnosis, i.e., core needle versus excision. In those instances, the pathology report is likely to contain language like ˵consistent with″, ˵suggestive of ″, ˵favor″ ˵cannot exclude″. One should take note of such language and confer with your pathologist to determine what precisely the issues are and what information she or he is trying to convey. When these words are used, the pathologist may be thinking that they have ˵communicated″ that the diagnosis is equivocal, but a non-pathologist may think otherwise. The best way to establish the most appropriate diagnosis is always using the ˵triple test″, clinical/radiologic/pathologic correlation. If the diagnoses or terms in the pathology report are not consistent with either the clinical and/or radiologic exam, further investigation is warranted. [ABSTRACT FROM AUTHOR]

A comparative study of extracellular DNA versus genomic or apoptotic DNA was executed by fluorescent in situ hybridization (FISH) analysis. Extracellular DNA from culture medium and bound to the cell surface of human primary endotheliocytes, human primary fibroblasts and HeLa cells were investigated. There were not any specific peculiarities found in extracellular DNA isolated from the culture medium of endotheliocytes and HeLa cells. We revealed an overrepresentation of chromosome 9 fragments and the regions of the short arms of chromosomes 13, 14, 15, 21, 22 in DNA isolated from the culture medium of primary fibroblasts. The pericentromeric region of chromosome 9 is also overrepresented in cell surface bound DNA isolated from endotheliocytes, fibroblasts and HeLa cells. The data obtained allow a rational selection of DNA targets for the investigation of extracellular DNA generation and circulating DNA-based diagnostics. [ABSTRACT FROM AUTHOR]