Search

Background: Black gram is one of the important pulse crops of the world and India is the major producer and consumer of pulses in the world. One of the main reason for low productivity of pulses is that they are cultivated mostly in areas with low soil moisture and poor soil fertility. In order to overcome such adverse conditions, low-cost techniques like seed hardening/pelleting are preferred. Seed pelleting provides package of nutrients that influences seed or soil at the seed-soil interface. Since the cost involved is very less, an attempt has been made to study the effect of botanical seed pelleting and foliar spary on growth and yield parameters in black gram cv. CO 6. Methods: Field experiments were conducted to evaluate the cumulative effect of botanical seed pelleting with pungam leaf powder @ 150g kg-1 and foliar spray of prosopis and moringa leaf powders with different concentrations (2%, 4%, 6% and 8%) on morpho physiological, leaf chlorophyll, gas exchange and yield parameters in black gram cv. CO 6 in the study. The observations on morpho physiological, leaf chlorophyll, gas exchange and yield parameters were recorded, statistically analysed and discussed here. Result: From the results, it was found that among the treatments, seed pelleting with pungam leaf powder @ 150 g kg-1 + foliar spray of prosopis leaf powder @ 6% recorded higher morpho physiological parameters viz., plant height (48.73 cm), number of branches plant-1 (8.78), dry matter production (14.83 g plant-1), chlorophyll 'a' (1.03 mg g-1), chlorophyll 'b' (1.11 mg g-1) and total chlorophyll content (2.14 mg g-1), photosynthesis and transpiration rates (27.33 mg CO2 m-2 s-1 and 10.60 mg H2O m-2 s-1), intercellular CO2 concentration and stomatal conductance (274.30 µ mol mol-1 and 0.82 mol-2s-1). This treatment also recorded lower number of days to first flowering (23.23 days), earliness in 50 per cent flowering (30.12 days) and also recorded higher yield parameters such as lengthier pods, more number of pods plant-1, pod yield plant-1, number of seeds pod-1, seed yield plant-1, seed yield ha-1 and hundred seed weight of 5.60 cm, 26.30, 8.20 g, 7.30, 5.30 g, 795.12 kg ha-1 and 5.83 g, respectively as compared to other treatments. To conclude, seed pelleting with pungam leaf powder @ 150 g kg-1 + foliar spray of prosopis leaf powder @ 6% proved to be the best in enhancing morpho physiological, growth and yield parameters. [ABSTRACT FROM AUTHOR]

Seed pelleting with leaf powders of two botanicals viz., pungam (Pongamia glabra) and prosopis (Prosopis juliflora) was done to study the effect of botanical seed pelleting on seedling quality, gas exchange, growth, yield and resultant seed quality parameters of black gram in the laboratory and field. From the results, it was found that, seeds pelleted with pungam leaf powder @ 150 g kg-1 recorded higher germination percentage (93.00), root length (18.35 cm), shoot length (19.34 cm), dry matter production (0.48 mg seedling-10) and vigour index (3505.10). The yield parameters such as pod length, number of pods plant-1, pod yield plant-1, number of seeds pod-1 and 100 seed weight were also found higher in pungam leaf powder pelleting treatment @ 150 g kg-1. The control recorded the least values in all these growth and yield parameters of black gram. [ABSTRACT FROM AUTHOR]

Isolation of intact, double stranded, pure and non- contaminated genomic DNA is prerequisite for large scale genotyping analysis including DNA-banks. Three methods of DNA isolation (Dellaporta, CTAB and Hi-PurAg DNA isolation kits) from 25 black gram genotypes were compared in terms of the yield, purity, integrity, and stability of extracted DNA. Purity and quantification of isolated DNA samples was confirmed by using the UV nano-spectrophotometer at OD260/280 and the same is confirmed based by agarose gel electrophoresis. The CTAB method showed the best results followed by Hi- PurAg and Dellaporta method. The CTAB DNA extraction method was found to be the most efficient DNA extraction method, capable of providing high quality, pure and stable DNA and could be used for various molecular related works. All the 25 black gram genotypes for this research gave good yield of DNA from the established modified CTAB protocol. [ABSTRACT FROM AUTHOR]

Investigations were carried out to study the influence of flyash seed pelleting on microbial populations of bacteria, fungi, actinomycetes, and rhizobium in black gram and green gram. The experimental results revealed that seed pelleting with fly ash seed pelleting @ 250 g with rice gruel as adhesive increased rhizobium population in black gram and fungi and actinomyctes population in green gram by 10 to 15%. Increased microbial activity will enrich soil microflora and in turn will be helpful in increasing the crop yield. [ABSTRACT FROM AUTHOR]

Ninety four genotypes of black gram were evaluated under rainfed condition for their biometric characters viz., plant height, number of pods plant-1, number of seeds pod-1, 100 seed weight and seed yield plant-1 for preliminary screening to identify the best performing genotype. Based on their yield performance, eight genotypes viz., RU8-705, PALAVAYALLOCAL, T 9, PHM 8, ADT 3, CBG-09-06, VANNIYUR-LOCAL and CBG-09-13 were selected and raised in three environments. Among them, two genotypes viz., T 9 and CBG-09-13 were selected and their root parameters viz., root and shoot length, shoot length, number of roots, root diameter, fresh weight of root, fresh weight of shoot, dry weight of root, dry weight of shoot, root volume and gas exchange characters viz., net photosynthetic rate, leaf temperature, stomatal conductance and intercellular CO2 were studied to identify the best genotype among them suitable for moisture stress condition. Based on the results, it was found that CBG-09-13 performed well under moisture stress condition. [ABSTRACT FROM AUTHOR]

Pulses are one of the most important legume crops in India contributing significantly high quality protein. Among the legumes, black gram (Vigna mungo L. Hepper), is an important legume crop in our country belonging to the family leguminoceae. The low productivity in pulses may be due to the fact that they are normally grown in marginal lands with inadequate soil moisture and poor fertility status. To increase yield under these circumstances, integrated seed treatment is given as a presowing treatment. Hence, a study was attempted to find out the influence of integrated seed treatments on growth and yield parameters in black gram. The seeds of black gram cv VBN 4 were subjected to various seed treatments and treated seeds along with the control were evaluated for their seed quality, growth, gas exchange and yield parameters both in laboratory and field trials. The study revealed that seeds fortified with MgSO4 + polykote + carbendazim + dimethoate + bioinoculant (Pseudomononas fluorescens) + Rhizobium + Azophos + pelleting with DAP recorded higher seed yield and other parameters when compared to control. [ABSTRACT FROM AUTHOR]

The present investigations were carried out to study the influence of various seed hardening treatments on growth and yield parameters in black gram under drought condition. The black gram cv.VBN 3 was imposed with various seed hardening treatments (i.e.,) MnSO4 @ 100 ppm, ZnSO4 @ 100 ppm, Cobalt nitrate @ 1 %, Sodium molybdate @ 100 ppm, KCl @ 100 ppm, Pungam leaf extract @ 1 %, Prosopis leaf extract @ 1% and GA3 @ 40 ppm. The above treated seeds along with control were evaluated for their seed quality parameters, growth, gas exchange and yield parameters under laboratory and drought field condition. The study revealed that seeds hardened with prosopis leaf extract @1% recorded higher seed yield, yield attributing characters and many seed quality characters as compared to other treatments and control under drought condition. [ABSTRACT FROM AUTHOR]

The present study was attempted to find out the influence of various seed hardening cum foliar spray treatments on growth and yield parameters in black gram under dry land condition. The seeds of black gram cv VBN 3 were imposed with hardening treatment with prosopis leaf extract @ 1%, and then they were again given with foliar spray treatments. The above treated seeds along with control were evaluated for their growth, physiological and yield parameters under field condition. The study revealed that, 1%, Prosopis leaf extract hardened seeds along with foliar spray of salicylic acid @ 100 ppm recorded the higher seed yield and other parameters when compared to other treatments and control. [ABSTRACT FROM AUTHOR]

Black gram is highly priced pulse, rich in protein (24%) and phosphoric acid. Besides being cooked for consumption with chepaties (rotis) and rice, it is also used in preparation of several South Indian dishes like idli, vada, dosa etc. In India, black gram is grown on an area of 2.07 million hectares with a production of 0.94 million tonnes. It is mainly grown in the States of Madhya Pradesh, Maharashtra, Uttar Pradesh, Rajasthan, Karnataka and Bihar. Seed is a biological entity. Flyash is generated during the combustion of coal in coal fired thermal power plants and paper industry. In agricultural studies, it has been reported that flyash at optimum rate can be utilized as a fertilizer. Hence, a study was formulated to elucidate information on the use of flyash seed pelleting on seed yield in blackgram. The study revealed that the flyash pelleting @ 200 g/kg-1 registered significantly higher values for plant height (cm), number of branches per plant, days to 50% flowering, number of clusters per plant, number of pods per plant, pod length, pod weight, seed weight/pod and seed yield plant-1 (g). [ABSTRACT FROM AUTHOR]

Black gram [Vigna mungo (L.) Hepper] is the third most important pulse crop in India after chickpea and pigeon pea. Soil salinity is one of the major factor responsible for loss in agricultural production. Forty eight black gram genotypes were evaluated for salt tolerance with six different salinity levels viz., EC0, EC4, EC7, EC10, EC13, EC16 at early seedling stage. All the seedling parameters and salt tolerant index were investigated from four days old seedlings. From the results, it was found that there was a gradual decrease with increasing levels of salinity in all the genotypes studied. Seed germination and dry matter production showed more variations than other parameters studied for all the genotypes at different salinity levels. Based on the results, it may be concluded that the genotypes VNBG 017, AUB 3 AND AUB 20 were saline tolerant and VNBG 022, AUB 31 AND AUB 12 were susceptible to salinity. [ABSTRACT FROM AUTHOR]

Here an efficient protocol for micropropagation of black gram [Vigna mungo (L.) Hepper] cv. VBN 3 is reported. The deembryonated cotyledonary explants were cultured on MS medium containing different concentrations of plant growth regulators. The maximum frequency (72%) of direct shoot regeneration (devoid of callus phase), multiple shoot induction and shoot elongation was achieved from culturing the explants on MS medium containing 3.0 mg/l of 6-benzylaminopurine (BAP). Up to 65% of the regenerated shoots were rooted on MS medium containing 0.25 mg/l of a-naphthalene acetic acid (NAA) within 3 weeks after subculturing. The in vitro-raised plantlets were successfully hardened first under culture room conditions with 62% survival rate and then in greenhouse. The identified regeneration system could be efficiently used in various in vitro manipulation studies in black gram as well. [ABSTRACT FROM AUTHOR]