1. In Vivo Newt Lens Regeneration Monitoring with Spectral-Domain Optical Coherence Tomography
- Author
-
chen, Weihao
- Subjects
- Biomedical Engineering, Optical Coherence Tomography, Medical Imaging, In vivo, Spectral-Domain Optical Coherence Tomography, Regenerative Medicine, Newt, Lens regeneration, Biomedical Engineering, Functional Imaging, Dedifferentiation, Transdifferentiation
- Abstract
Newts have exceptional capability of regenerating the lens throughout their lifetime. Since the 1890s, lens regeneration has been documented with ex vivo imaging techniques only. For the first time, we demonstrate that Optical Coherence Tomography (SD-OCT) can capture the in vivo essential morphological characteristics with abundant dynamic features. Monitoring lens regeneration using a single newt is now possible. The results show that the lens originates below the pupillary margin of the middle dorsal region and its early regenerating lens has an irregular elliptical shape. The lens volume expands quadratically, where its regenerating rate is linear from 14 to 60 days post-lentectomy. These findings warrant future research for tailoring OCT to study newt lens regeneration in vivo dynamically.
- Published
- 2021