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In drug development, ADME issues are still large contributors to drug attrition. The use of in vitro systems, such as microsomes and hepatocytes, for the assessment of metabolic stability and prediction of in vivo clearance, are well established for substrates of the cytochrome P450 enzymes (CYPs). However, these systems have been less explored for conjugation reactions, such as glucuronidation by uridine diphosphate glucuronosyltransferases (UGTs). The aim of this study was to increase the understanding of glucuronidation kinetics in vitro and the factors influencing the prediction of metabolic clearance by UGTs. more...