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The immune generation of cytotoxic T lymphocytes (CTLs) for killing tumor cells has a critical step of cross-presentation, in which the tumor cells or tumor antigens are taken up by professional antigen presenting cells (APCs), processed and presented to naive CD8+ T cells. Some professional APCs may express B7 co-stimulator to provide secondary signals for the differentiation of the naive CD8+ T cells and may stimulate naive CD4+ T cells to be helper T cells for CTL development. The differentiated CD8+cytotoxic effector T cells are specific to some tumor-associated peptide antigens. These effector CD8+ T cells may effectively kill the tumor cells presenting tumor-associated peptide antigens without the need for co-stimulator B7 molecules. Hence, an immunogenic glycopeptide in complex with MHC class I has been targeted for activation of anti-tumor CTLs. A liposome based cancer vaccine consisting of a 20-amino-acid MUC1 peptide containing MUC1 glycopeptide GalNAc-O-Thr (Tn) has been decorated with L-rhamnose-cholesterol and conjugated to a functionalized Toll-like receptor ligand (TLRL), Pam3Cys. In the presence of anti-rhamnose antibody, this induces Fc-Fc¿R interaction to enhance antigen uptake. CD4+ helper T cells were activated by the liposomal vaccination in Rha-Ficoll immunized BALB/c mice, but CD8+ CTLs were not stimulated effectively by the initial liposomal vaccine. I have tested a different peptide proposed to contain a CD8+ T cell epitope, which is a small peptide that contains 8 amino-acids of the MUC1 sequence. The small 8 amino-acid glycopeptide has GalNAc-O-Thr (Tn) at a different position than in the CD4+ T cell epitope. The GalNAc-O-Thr (Tn) in complex with the MHC class I of APCs works effectively as an anchor residue for positive bonding within the pocket of the MHC class I molecule. The successful cross-presentation process causes proliferation of cytotoxic T lymphocytes which can kill specific tumor cells. A JAM assay shows the capability to kill the tumor cells. Induction of CD8+ T cells was measured by the level of intracellular or extracellular type II interferon (IFN-¿), which is a cytokine for tumor control. An intracellular staining method was used to fix the intracellular IFN-¿ inside of the cell and analyze the level of IFN-¿ with fluorescence. An IFN-¿ ELISA assay was used to measure extracellular IFN-¿ in the cell supernatant. It was shown that the use of anti-rhamnose antibody to enhance vaccine uptake caused an increase in CD8+ T cell response in addition to an increased antibody response.

In our current economic state, instant gratification from the satisfaction by the real time products and services and experiences are in demand for consumers. As a result, there are several if not many of these real-time base services that have arisen from what the consumers' desire. During the research, I have witnessed the phenomenon that would fit the criteria. The perfect example would be the popularity of meals on wheels in the United States. Setting a new trend in the fast food market, the food truck industry has reached the numbers well over the thousands and still counting with their own unique ideas and innovations. I can confidently say, with the rise of the food truck industry, we have observed the decline of stationary restaurants. But, unlike the mobile counterpart, the stationary restaurants still have one distinct advantage; consumers know where to find them. For example, when struck with a craving for tacos, it is easier to Google a Mexican restaurant down the street, rather than to track down a taco truck. To counterstrike the stationary opposition to quench the hunger for the food truck enthusiasts, several apps have been created. The applications general idea is to pin point gourmet food trucks on mobile maps to even the reliability of playing fields via Twitter feeds, GPS and truck-reported location data. While none of which has achieved an exhaustive or completely accurate system, the search still continues for trucks for users depending on their location. During the present market condition, I am certain of making an accurate and effective real-time information service would be an interesting subject to approach in satisfying users and business owners desires. In demand of real-time information services, I will create a prototype for a food truck information service, inclusive of real-time location service; GPS, mobile tracking, truck-reported data and alert service. The consumers and the food truck owners will both come out as winners, with relaying precise information via real time communication devices. As a student studying the art of user experience and interaction design, goal of this study is to figure out how to enhance the user friendly interface along with meeting the expectations of actual consumers. In order to have a deeper understanding about interaction between users and real time location applications to heighten the level of services, I am willing to go above and beyond with through research to develop a next generation real time app during this project. Another critical factor that I, a user experience designer would point out would be communication. A key factor in completing the task, finding a significant way of communication method would be an additional goal throughout this project.