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Incisional hernias are areas of weakness in the tissue caused by a surgical wound that doesn’t heal completely. This study examines the contributions of mesenchymal stromal cells and platelet-rich plasma on wound repair by observing wound healing of the abdominal muscle in a rat model. A cell adhesion assay was performed to determine which mesh material MSCs bind to most effectively MSCs/PRP/mesh material were applied to a repaired surgical incision, after which, the rat recovered for 4 to 8 weeks. The abdominal muscle was then sectioned, paraffin embedded, and placed on slides to be stained with Mason’s trichrome. The thickness of collagen deposition was measured using Motic ImagesPlus 2.0. Collagen organization, abundance, and myocyte degeneration were also evaluated by a panel of 4 blind observers. Changes in the thickness of collagen deposition were not demonstrated, but neovascularization and collagen abundance in the muscle layer was significantly increased with the addition of MSC and PRP during wound healing. Increased collagen at the wound site may improve the overall tissue strength following wound repair.