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In this paper, we use GIS and 2D geometric morphometrics to explore landscape use and social interaction among Kirk Cluster populations in the lower Ohio River valley. Using cultural transmission as a theoretical foundation, we develop models for identifying assemblages produced by macroband aggregations. We show that two distinct populations occupied northern Indiana and southwestern Kentucky. Intensively occupied sites in these areas are situated in near-upland settings in close proximity to a variety of resources including chert, higher order rivers, and sinkholes. In contrast, the Butterfield site in central Kentucky lies in the lowlands with the Green River as the only obvious resource. Analyses reveal that Butterfield was a macroband aggregation site visited by populations from Indiana, but groups from southwestern Kentucky only minimally participated in aggregations at Butterfield. Results further show that the Ohio River was not a barrier to social interaction in the Early Holocene. [ABSTRACT FROM AUTHOR]