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This 21st Century Skills Map is the result of hundreds of hours of research, development and feedback from educators and business leaders across the nation. The Partnership for 21st Century Skills has issued this map for the core subject of the Arts.

To assess the long-term impacts of adult literacy programs, the Center for Literacy Studies conducted the Longitudinal Study of Adult Literacy Participants in Tennessee from 1991-95. The study focused on changes in the lives of 450 participants in the domains of work, family, and community after they enrolled in literacy programs. Participants from three cohorts (1991-92, 1992-93, 1993-94) were enrolled at literacy Level One, and their initial scores on the Adult Basic Learning Exam reading test were below the sixth-grade level. Follow-up studies were administered annually through 1995, although the number of participants who could be located diminished each year. Results were analyzed from the responses of 199 adults from the 3 cohorts who took part in a follow-up interview approximately 1 year after their initial enrollment. The 116 questions dealt with employment, literacy practices, involvement with children's schooling, community awareness, self-esteem, and life satisfaction. Participants reported positive change on at least one item in each category, including a higher rate of employment, increased self-esteem, increased involvement in community organizations, and increases in some uses of literacy. Data showed no significant changes in community awareness or in how people felt about their community; no greater likelihood of attending community meetings or talking about politics; no significant increase in reading or in involvement in children's education; and few significant changes in life satisfaction. (Contains 16 references.) (YLB)

Students in a laboratory techniques course for preservice teachers were engaged in an activity that was designed to assess their skills in identifying geologic materials and model a situation that might be encountered by new teachers. Related activities were designed to give the preservice teachers practical experience in obtaining geologic specimens, labeling them and storing collections. (Author/MM)

This manual was developed to provide a model for evaluating programs for handicapped preschoolers that can be used by all Maryland school districts regardless of size or resources. The model has five components: (1) how children are identified as handicapped; (2) what type of testing takes place before and after a child is placed in a program; (3) procedures for determining a child's program placement; (4) program effectiveness; and (5) program management of communication and recordkeeping procedures. The manual has four sections. The Overview familiarizes the reader with the model and how it can be used. The Evaluation Model section details the model's components and subcomponents, plus provides evaluation questions, data collection instruments, and suggestions for adapting and using the instruments. The third section, Managing Data, offers suggestions for collecting data, preparing data for analysis, analyzing the data, and writing up results. The Appendices contain a glossary of terms, an annotated bibliography, descriptions of different instrument types, information on instrument format, codes and identification numbers, a sample checklist and letters, information on how to calculate, establish and maintain inter-observer reliability, and sample summary reports. The model is presented as a menu from which users may pick and choose, depending on their interests and experience. (BS)

Describes reform efforts in science education at Northern Arizona University that emphasize active, inquiry-based learning in introductory biology classes. Recommends the use of a number of strategies to involve large classes in thinking about and doing science. Contains 20 references. (DDR)

Intended for those responsible for all kinds of publications in developing countries, this booklet has three main objectives: (1) to give the ordinary person who knows nothing about printing or printing processes enough information to be able to deal intelligently with a printer, (2) to show how it is possible for a person or group of persons with no previous experience to set up their own printing operation, and (3) to demonstrate how modest publications from leaflets to small booklets and even rural newspapers serving particular groups of people can be produced. Various sections of the booklet deal with relief block, rubber printing, letterpress, linotype, offset litho, stencil duplicating, dealing with the printer, starting one's own publication, getting quotations, preparing the manuscript, proofreading, and pagination. (HOD)

This guide is intended to answer employers' questions on how to hire and manage workers who have an intellectual impairment. Following an introductory description and definition of intellectual impairment, the guide addresses relevant issues through a series of responses to questions: (1) Do individuals with an intellectual impairment make good employees?; (2) What are their job strengths and weaknesses?; (3) Are there any special considerations for employees who work in jobs requiring contact with customers or the public?; (4) What kinds of jobs can they perform?; (5) Will extra training time or supervision be required?; (6) How can training or supervision be provided without disrupting the flow of activity?; (7) Should an employee with an impairment be treated differently from other employees?; (8) How will co-workers respond to the new employee?; (9) What are the costs associated with hiring intellectually impaired workers?; (10) Will company insurance rates change?; and (11) What are the benefits associated with hiring intellectually impaired workers? Names and addresses of organizations that can supply additional information are provided along with a summary of "keys to success" that encourage employer commitment, education, understanding, and active partnership with vocational rehabilitation specialists and agencies. (VW)

Intended for journalists in developing countries, this manual gives a detailed account of the functions carried out in the newsroom. The first section deals with aspects of the reporter's job, including news writing and stylistics, interviewing, and press law. The second section discusses the duties of the news editor, including maintaining perennial sources, thinking ahead, following up on hard news items, promoting creative angles to news stories, and cultivating news from rural areas. The third section covers components of subediting, such as design and layout, typography, headline and caption writing, and elements of style. The fourth section explores developing ideas for feature writing, while the last section outlines principles of photo editing. (HTH)

The paper examines the prospects of employment for Canadian youth with mental disabilities and offers a model for secondary vocational programing designed to promote the transition from school to work. An introductory section comments on the bleak outlook for employment for students with disabilities and notes the economic and social consequences. The lack of secondary programing initiatives in Canada is noted; programs that do exist concentrate on training students with mild disabilities and academic motivation problems. The need is noted for extending prevocational training into the upper elementary grades and developing structured vocational programs at junior high levels followed by supervised work experience and school-to-work transition programs and senior high school. A comprehensive secondary vocational program model is espoused which includes modules for entry assessment, prevocational skills, work adjustment, work experience, and transition services. The model is designed to help students acquire skills necessary for working and to bridge the gap between students' school experiences and their post-school environment through school-community linkages. The government of Canada's role in promoting an integrated continuum of services is addressed. Six pages of references are appended. (CL)

Populations of rusty grain beetle, Cryptolestes ferrugineus, have developed a very high level of resistance (1300×) to the fumigant phosphine (PH3) in Australia. Resistant insects triggered control failures, threatening the country's annual grain market worth AU$8 billion. Although PH3 protocols were amended to manage this new resistance, fumigation requires lengthy exposure periods which has practical difficulties. While there is no suitable replacement for PH3, the current study explores potential approaches to enhance the efficacy of this fumigant. One possibility is co-fumigation of PH3 with another complementary fumigant, sulfuryl fluoride (SO2F2 or SF), with the dual goals: enhanced efficacy and minimise use of both fumigants. A cohort of mixed age eggs and adults of PH3-resistant C. ferrugineus was fumigated with PH3 and SF individually, as well as in combination inside desiccators at 25°C and 60%RH for 168 h. Two doses below the maximal registered rates for SF (8.9 mg L-1, equivalent to 1500 g hm-3) and PH3 (1.0 mg L-1) were tested. Co-fumigation was performed simultaneously for 168 h. Our results revealed that, the mixture of 1.1 mg L-1 or 2.2 mg L-1 of SF and 0.5 mg L-1 of PH3 over 168 h achieved complete control against resistant C. ferrugineus eggs and adults, whereas each of the tested doses failed individually. Our study confirms that SF and PH3 enhance the efficacy of each other when used in combination, which holds great potential for managing resistant C. ferrugineus.

A major concern for the Australian grain industry in recent years is the constant threat of resistance to the key disinfectant phosphine in a range of stored grain pests. The need to maintain the usefulness of phosphine and to contain the development of resistance are critical to international market access for Australian grain. Strong levels of resistance have already been established in major pests including the lesser grain borer, Rhyzopertha dominica (F.), the red flour beetle, Tribolium castaneum (Herbst), and most recently in the rusty grain beetle Cryptolestes ferrugineus (Stephens). As a proactive integrated resistance management strategy, new fumigation protocols are being developed in the laboratory and verified in large-scale field trials in collaboration with industry partners. To aid this development, we have deployed advanced molecular diagnostic tools to accurately determine the strength and frequency of key phosphine resistant insect pests and their movement within a typical Australian grain value chain. For example, two major bulk storage facilities based at Brookstead and Millmerran in southeast Queensland, Australia, were selected as main nodes and several farms and feed mills located in and around these two sites at a scale of 25 to 100 km radius were selected and surveyed. We determined the type, pattern, frequency as well as the distribution of resistance alleles accurately for two major pests, R. dominica and T. castaneum. Overall, this information along with the phenotypic data, provide a basis for designing key intervention strategies in managing resistance problems in the study area.

BACKGROUND: Next-generation sequencing can enable genetic surveys of large numbers of individuals. We developed a genotyping-by-sequencing assay for detecting strong phosphine resistance alleles in the dihydrolipoamide dehydrogenase (dld) gene of Rhyzopertha dominica populations. The assay can estimate the distribution and frequency of resistance variants in thousands of individual insects in a single run. RESULTS: We analysed 1435 individual insects collected over a 1-year period from 59 grain-storage sites including farms (n = 29) and central storages (n = 30) across eastern Australia. Resistance alleles were detected in 49% of samples, 38% of farms and 60% of central storages. Although multiple alleles were detected, only two resistance variants (P49S and K142E) were widespread and each appeared to have a distinct but overlapping geographical distribution. CONCLUSION: The type of structure in which the grain is stored had a strong effect on resistance allele frequency. We observed higher frequencies of resistance alleles in bunker storages at central sites compared with other storage types. This contributed to the higher frequencies of resistance alleles in bulk-handling facilities relative to farms. The discovery of a storage structure that predisposes insects to resistance highlights the utility of our high-throughput assay system for improvement of phosphine resistance management practices. © 2018 Society of Chemical Industry. © 2018 Society of Chemical Industry

Phosphine resistance monitoring typically involves bioassays of beetles from population samples collected from grain storage facilities. Insects are classified into susceptible or resistant phenotypes based on mortality or survival at one or more discriminating doses. Although valuable, phenotype testing has several drawbacks. First, phenotype testing needs live insects, and considerable effort is required to collect and maintain them before testing. Second, population samples may contain multiple genotypes expressing different levels of resistance that may not be distinguishable using discriminating dose bioassays. Third, collections are likely to be focussed around grain storages to maximise sampling success. Recent research shows that several key pests are actively dispersing through flight. The availability of commercial pheromone lures and recent advances in molecular screening provide an opportunity to provide information on resistance gene frequencies more broadly across the landscape. This approach is proving to be a valuable adjunct to traditional resistance testing in Australia.

Contains fulltext : 165947.pdf (publisher's version ) (Open Access)

Strong resistance to phosphine is an increasing problem, with Cryptolestes ferrugineus requiring very high doses or extended fumigation times to achieve control. As a result, sulfuryl fluoride (SF) is used as an alternative fumigant, but its use is limited by cost and the potential for residues. To overcome these limitations, we have tested both simultaneous and sequential co-treatment with phosphine and SF. Both strategies control all life stages of the four major grain pests more rapidly than phosphine and at reduced chemical concentrations, reducing cost and potential residues. We have reinvestigated the model of SF toxicity that has prevailed for more than 50 years - that fluoride released from SF is the toxic entity that kills both vertebrate and invertebrate organisms. We have clearly demonstrated that this mechanism is false. We find that fluorosulfate, which is also released from SF, is not only much more toxic than fluoride, but acts much more rapidly as well. Based on the mechanism of action of phosphine, we have identified a range of potent synergists. We also find evidence that resistance to phosphine is associated with permanent activation of stress defence systems, which actually limits the adaptability of resistant animals. We are in the preliminary stages of testing a synergistic gas using our knowledge to maximise its effectiveness.

Fumigation with phosphine gas is the primary method of controlling stored grain pests. In Turkey, phosphine has been used extensively since the 1950's. Even though high levels of phosphine resistance have been detected in several key stored products pests across the world, it has never been studied in Turkey despite this long history of phosphine use. High-level phosphine resistance has been detected and genetically characterised previously in the rust red flour beetle, Tribolium castaneum in other countries. Since this pest is also a common problem in stored grain environment in Turkey, the current study was undertaken for the first time, to investigate the distribution and strength of phosphine resistance in T. castaneum. Four strains of T. castaneum were tested through bioassays for determining the weak and strong phosphine resistance phenotypes on the basis of the response of adults to discriminating phosphine concentrations of 0.03 mg/L and 0.25 mg/L, for 20 hour exposures respectively. Phenotype testing showed all strains exhibited some level of phosphine resistance with a maximum level of 196 fold. Sequencing and genetic testing of seven field-collected strains showed that all of them carried a strong resistance allele in at the rph2 locus similar to the one previously reported. Our results show that strong resistance to phosphine is common in Turkish strains of T. castaneum.

Phosphine (PH3) fumigation is the primary method worldwide for controlling insect pests of stored commodities. Over-reliance on phosphine, however, has led to the emergence of strong resistance. Detailed genetic studies previously identified two loci, rph1 and rph2, that interact synergistically to create a strong resistance phenotype. We compared the genetics of phosphine resistance in strains of Rhyzopertha dominica and Tribolium castaneum from India and Australia, countries having similar pest species but widely differing in pest management practices. Sequencing analysis of the rph2 locus, dihydrolipoamide dehydrogenase (dld), identified two structurally equivalent variants, Proline49>Serine (P49S) in one R. dominica strain and P45S in three strains of T. castaneum from India. These variants of the DLD protein likely affect FAD cofactor interaction with the enzyme. A survey of insects from storage facilities across southern India revealed that the P45/49S variant is distributed throughout the region at very high frequencies, in up to 94% of R. dominica and 97% of T. castaneum in the state of Tamil Nadu. The abundance of the P45/49S variant in insect populations contrasted sharply with the evolutionary record in which the variant was absent from eukaryotic DLD sequences. This suggests that the variant is unlikely to provide a strong selective advantage in the absence of phosphine fumigation.

Phosphine resistance alleles might be expected to negatively affect energy demanding activities such as walking and flying, because of the inverse relationship between phosphine resistance and respiration. We used an activity monitoring system to quantify walking of Rhyzopertha dominica (F.) and a flight chamber to estimate their propensity for flight initiation. No significant difference in the duration of walking was observed between the strongly resistant, weakly resistant, and susceptible strains of R. dominica we tested, and females walked significantly more than males regardless of genotype. The walking activity monitor revealed no pattern of movement across the day and no particular time of peak activity despite reports of peak activity of R. dominica and Tribolium castaneum (Herbst) under field conditions during dawn and dusk. Flight initiation was significantly higher for all strains at 28 degrees C and 55% relative humidity than at 25, 30, 32, and 35 degrees C in the first 24 h of placing beetles in the flight chamber. Food deprivation and genotype had no significant effect on flight initiation. Our results suggest that known resistance alleles in R. dominica do not affect insect mobility and should therefore not inhibit the dispersal of resistant insects in the field.

BACKGROUND: The lesser grain borer, Rhyzopertha dominica (F.), is a highly destructive pest of stored grain that is strongly resistant to the fumigant phosphine (PH3). Phosphine resistance is due to genetic variants at the rph2 locus that alter the function of the dihydrolipoamide dehydrogenase (DLD) gene. This discovery now enables direct detection of resistance variants at the rph2 locus in field populations. RESULTS: A genotype assay was developed for direct detection of changes in distribution and frequency of a phosphine resistance allele in field populations of R. dominica. Beetles were collected from ten farms in south-east Queensland in 2006 and resampled in 2011. Resistance allele frequency increased in the period from 2006 to 2011 on organic farms with no history of phosphine use, implying that migration of phosphine-resistant R. dominica had occurred from nearby storages. CONCLUSION: Increasing resistance allele frequencies on organic farms suggest local movement of beetles and dispersal of insects from areas where phosphine has been used. This research also highlighted for the first time the utility of a genetic DNA marker in accurate and rapid determination of the distribution of phosphine-resistant insects in the grain value chain. Extending this research over larger landscapes would help in identifying resistance problems and enable timely pest management decisions. © 2013 Society of Chemical Industry © 2013 Society of Chemical Industry 69 6 June 2013 10.1002/ps.3514 Rapid Report Rapid Report © 2013 Society of Chemical Industry.

The lesser grain borer Rhyzopertha dominica (F.) is one of the most destructive insect pests of stored grain. This pest has been controlled successfully by fumigation with phosphine for the last several decades, though strong resistance to phosphine in many countries has raised concern about the long term usefulness of this control method. Previous genetic analysis of strongly resistant (SR) R. dominica from three widely geographically dispersed regions of Australia, Queensland (SRQLD), New South Wales (SRNSW) and South Australia (SRSA), revealed a resistance allele in the rph1 gene in all three strains. The present study confirms that the rph1 gene contributes to resistance in a fourth strongly resistant strain, SR2(QLD), also from Queensland. The previously described rph2 gene, which interacts synergistically with rph1 gene, confers strong resistance on SRQLD and SRNSW. We now provide strong circumstantial evidence that weak alleles of rph2, together with rph1, contribute to the strong resistance phenotypes of SRSA and SR2(QLD). To test the notion that rph1 and rph2 are solely responsible for the strong resistance phenotype of all resistant R. dominica, we created a strain derived by hybridising the four strongly resistant lines. Following repeated selection for survival at extreme rates of phosphine exposure, we found only slightly enhanced resistance. This suggests that a single sequence of genetic changes was responsible for the development of resistance in these insects.

Phosphine is a small redox-active gas that is used to protect global grain reserves, which are threatened by the emergence of phosphine resistance in pest insects. We find that polymorphisms responsible for genetic resistance cluster around the redox-active catalytic disulfide or the dimerization interface of dihydrolipoamide dehydrogenase (DLD) in insects (Rhyzopertha dominica and Tribolium castaneum) and nematodes (Caenorhabditis elegans). DLD is a core metabolic enzyme representing a new class of resistance factor for a redox-active metabolic toxin. It participates in four key steps of core metabolism, and metabolite profiles indicate that phosphine exposure in mutant and wild-type animals affects these steps differently. Mutation of DLD in C. elegans increases arsenite sensitivity. This specific vulnerability may be exploited to control phosphine-resistant insects and safeguard food security.

Fumigation of stored grain with phosphine (PH 3) is used widely to control the lesser grain borer Rhyzopertha dominica. However, development of high level resistance to phosphine in this species threatens control. Effective resistance management relies on knowledge of the expression of resistance in relation to dosage at all life stages. Therefore, we determined the mode of inheritance of phosphine resistance and strength of the resistance phenotype at each developmental stage. We achieved this by comparing mortality and developmental delay between a strongly resistant strain (R-strain), a susceptible strain (S-strain) and their F 1 progenies. Resistance was a maternally inherited, semi-dominant trait in the egg stage but was inherited as an autosomal, incompletely recessive trait in larvae and pupae. The rank order of developmental tolerance in both the sensitive and resistant strains was eggs > pupae > larvae. Comparison of published values for the response of adult R. dominica relative to our results from immature stages reveals that the adult stage of the S-strain is more sensitive to phosphine than are larvae. This situation is reversed in the R-strain as the adult stage is much more resistant to phosphine than even the most tolerant immature stage. Phosphine resistance factors at LC 50 were eggs 400×, larvae 87× and pupae 181× with respect to reference susceptible strain (S-strain) adults indicating that tolerance conferred by a particular immature stage neither strongly nor reliably interacts with the genetic resistance element. Developmental delay relative to unfumigated control insects was observed in 93% of resistant pupae, 86% of resistant larvae and 41% of resistant eggs. Increased delay in development and the toxicity response to phosphine exposure were both incompletely recessive. We show that resistance to phosphine has pleiotropic effects and that the expression of these effects varies with genotype and throughout the life history of the insect

Phosphine is the only economically viable fumigant for routine control of insect pests of stored food products, but its continued use is now threatened by the world-wide emergence of high-level resistance in key pest species. Phosphine has a unique mode of action relative to well-characterised contact pesticides. Similarly, the selective pressures that lead to resistance against field sprays differ dramatically from those encountered during fumigation. The consequences of these differences have not been investigated adequately. We determine the genetic basis of phosphine resistance in Rhyzopertha dominica strains collected from New South Wales and South Australia and compare this with resistance in a previously characterised strain from Queensland. The resistance levels range from 225 and 100 times the baseline response of a sensitive reference strain. Moreover, molecular and phenotypic data indicate that high-level resistance was derived independently in each of the three widely separated geographical regions. Despite the independent origins, resistance was due to two interacting genes in each instance. Furthermore, complementation analysis reveals that all three strains contain an incompletely recessive resistance allele of the autosomal rph1 resistance gene. This is particularly noteworthy as a resistance allele at rph1 was previously proposed to be a necessary first step in the evolution of high-level resistance. Despite the capacity of phosphine to disrupt a wide range of enzymes and biological processes, it is remarkable that the initial step in the selection of resistance is so similar in isolated outbreaks.

Phosphine is a small redox-active gas that is used to protect global grain reserves, which are threatened by the emergence of phosphine resistance in pest insects. We find that polymorphisms responsible for genetic resistance cluster around the redox-active catalytic disulfide or the dimerization interface of dihydrolipoamide dehydrogenase (DLD) in insects (Rhyzopertha dominica and Tribolium castaneum) and nematodes (Caenorhabditis elegans). DLD is a core metabolic enzyme representing a new class of resistance factor for a redox-active metabolic toxin. It participates in four key steps of core metabolism, and metabolite profiles indicate that phosphine exposure in mutant and wild-type animals affects these steps differently. Mutation of DLD in C. elegans increases arsenite sensitivity. This specific vulnerability may be exploited to control phosphine-resistant insects and safeguard food security.

Contains reports on two research projects., National Science Foundation (Grant GP-2495), National Institutes of Health (Grant MH-04737-04), National Aeronautics and Space Administration (Grant NsG-334), National Aeronautics and Space Administration (Grant NsG-496)

Existing procedures for the generation of polymorphic DNA markers are not optimal for insect studies in which the organisms are often tiny and background molecular Information is often non-existent. We have used a new high throughput DNA marker generation protocol called randomly amplified DNA fingerprints (RAF) to analyse the genetic variability In three separate strains of the stored grain pest, Rhyzopertha dominica. This protocol is quick, robust and reliable even though it requires minimal sample preparation, minute amounts of DNA and no prior molecular analysis of the organism. Arbitrarily selected oligonucleotide primers routinely produced similar to 50 scoreable polymorphic DNA markers, between individuals of three Independent field isolates of R. dominica. Multivariate cluster analysis using forty-nine arbitrarily selected polymorphisms generated from a single primer reliably separated individuals into three clades corresponding to their geographical origin. The resulting clades were quite distinct, with an average genetic difference of 37.5 +/- 6.0% between clades and of 21.0 +/- 7.1% between individuals within clades. As a prelude to future gene mapping efforts, we have also assessed the performance of RAF under conditions commonly used in gene mapping. In this analysis, fingerprints from pooled DNA samples accurately and reproducibly reflected RAF profiles obtained from Individual DNA samples that had been combined to create the bulked samples.

The evaluation of the integrity of the Air Traffic Control Radar Beacon System (ATCRBS) signal format for the Beacon Collision Avoidance System (BCAS) data link was based on measurements of the actual RF environment today, simulations of sophisticated signal processors, and basic calculations. The conclusions reached by the task force all relate to achieving a high integrity data link tailored to the BCAS application and were derived from tests run on the DABS ground-based reply processor-they are the following: (1) A data link with a high degree of error protection coding is essential. (2) Multiple transmissions - itself a form of coding - is essential. (3) A two-way data link is highly desirable from the point of view of the coordination logic. (Author)