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28 results on '"Fréal A"'

1. Sodium channel endocytosis drives axon initial segment plasticity

Author

Fréal, Amélie, Jamann, Nora, Ten Bos, Jolijn, Jansen, Jacqueline, Petersen, Naomi, Ligthart, Thijmen, Hoogenraad, Casper C, Kole, Maarten H P, Fréal, Amélie, Jamann, Nora, Ten Bos, Jolijn, Jansen, Jacqueline, Petersen, Naomi, Ligthart, Thijmen, Hoogenraad, Casper C, and Kole, Maarten H P

Abstract

Activity-dependent plasticity of the axon initial segment (AIS) endows neurons with the ability to adapt action potential output to changes in network activity. Action potential initiation at the AIS highly depends on the clustering of voltage-gated sodium channels, but the molecular mechanisms regulating their plasticity remain largely unknown. Here, we developed genetic tools to label endogenous sodium channels and their scaffolding protein, to reveal their nanoscale organization and longitudinally image AIS plasticity in hippocampal neurons in slices and primary cultures. We find that N-methyl-d-aspartate receptor activation causes both long-term synaptic depression and rapid internalization of AIS sodium channels within minutes. The clathrin-mediated endocytosis of sodium channels at the distal AIS increases the threshold for action potential generation. These data reveal a fundamental mechanism for rapid activity-dependent AIS reorganization and suggests that plasticity of intrinsic excitability shares conserved features with synaptic plasticity.

Published
2023

2. Sodium channel endocytosis drives axon initial segment plasticity

Author

Cell Biology, Neurobiology and Biophysics, Sub Cell Biology, Fréal, Amélie, Jamann, Nora, Ten Bos, Jolijn, Jansen, Jacqueline, Petersen, Naomi, Ligthart, Thijmen, Hoogenraad, Casper C, Kole, Maarten H P, Cell Biology, Neurobiology and Biophysics, Sub Cell Biology, Fréal, Amélie, Jamann, Nora, Ten Bos, Jolijn, Jansen, Jacqueline, Petersen, Naomi, Ligthart, Thijmen, Hoogenraad, Casper C, and Kole, Maarten H P

Published
2023

3. Sodium channel endocytosis drives axon initial segment plasticity

Author

Fréal, Amélie, Jamann, Nora, Ten Bos, Jolijn, Jansen, Jacqueline, Petersen, Naomi, Ligthart, Thijmen, Hoogenraad, Casper C, Kole, Maarten H P, Fréal, Amélie, Jamann, Nora, Ten Bos, Jolijn, Jansen, Jacqueline, Petersen, Naomi, Ligthart, Thijmen, Hoogenraad, Casper C, and Kole, Maarten H P

Abstract

Activity-dependent plasticity of the axon initial segment (AIS) endows neurons with the ability to adapt action potential output to changes in network activity. Action potential initiation at the AIS highly depends on the clustering of voltage-gated sodium channels, but the molecular mechanisms regulating their plasticity remain largely unknown. Here, we developed genetic tools to label endogenous sodium channels and their scaffolding protein, to reveal their nanoscale organization and longitudinally image AIS plasticity in hippocampal neurons in slices and primary cultures. We find that N-methyl-d-aspartate receptor activation causes both long-term synaptic depression and rapid internalization of AIS sodium channels within minutes. The clathrin-mediated endocytosis of sodium channels at the distal AIS increases the threshold for action potential generation. These data reveal a fundamental mechanism for rapid activity-dependent AIS reorganization and suggests that plasticity of intrinsic excitability shares conserved features with synaptic plasticity.

Published
2023

4. TRIM46 Organizes Microtubule Fasciculation in the Axon Initial Segment

Author

Harterink, Martin, Vocking, Karin, Pan, Xingxiu, Soriano Jerez, Eva M., Slenders, Lotte, Fréal, Amélie, Tas, Roderick P., Van De Wetering, Willine J., Timmer, Karina, Motshagen, Jasmijn, Van Beuningen, Sam F.b., Kapitein, Lukas C., Geerts, Willie J.c., Post, Jan A., Hoogenraad, Casper C., Harterink, Martin, Vocking, Karin, Pan, Xingxiu, Soriano Jerez, Eva M., Slenders, Lotte, Fréal, Amélie, Tas, Roderick P., Van De Wetering, Willine J., Timmer, Karina, Motshagen, Jasmijn, Van Beuningen, Sam F.b., Kapitein, Lukas C., Geerts, Willie J.c., Post, Jan A., and Hoogenraad, Casper C.

Abstract

Selective cargo transport into axons and dendrites over the microtubule network is essential for neuron polarization. The axon initial segment (AIS) separates the axon from the somatodendritic compartment and controls the microtubule-dependent transport into the axon. Interestingly, the AIS has a characteristic microtubule organization; it contains bundles of closely spaced microtubules with electron dense cross-bridges, referred to as microtubule fascicles. The microtubule binding protein TRIM46 localizes to the AIS and when overexpressed in non-neuronal cells forms microtubule arrays that closely resemble AIS fascicles in neurons. However, the precise role of TRIM46 in microtubule fasciculation in neurons has not been studied. Here we developed a novel correlative light and electron microscopy approach to study AIS microtubule organization. We show that in cultured rat hippocampal neurons of both sexes, TRIM46 levels steadily increase at the AIS during early neuronal differentiation and at the same time closely spaced microtubules form, whereas the fasciculated microtubules appear at later developmental stages. Moreover, we localized TRIM46 to the electron dense cross-bridges and show that depletion of TRIM46 causes loss of cross-bridges and increased microtubule spacing. These data indicate that TRIM46 has an essential role in organizing microtubule fascicles in the AIS.SIGNIFICANCE STATEMENT The axon initial segment (AIS) is a specialized region at the proximal axon where the action potential is initiated. In addition the AIS separates the axon from the somatodendritic compartment, where it controls protein transport to establish and maintain neuron polarity. Cargo vesicles destined for the axon recognize specialized microtubule tracks that enter the AIS. Interestingly the microtubules entering the AIS form crosslinked bundles, called microtubule fascicules. Recently we found that the microtubule-binding protein TRIM46 localizes to the AIS, where it may organize

Published
2019

5. Feedback-Driven Assembly of the Axon Initial Segment

Author

Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, Hoogenraad, Casper C, Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, and Hoogenraad, Casper C

Abstract

The axon initial segment (AIS) is a unique neuronal compartment that plays a crucial role in the generation of action potential and neuronal polarity. The assembly of the AIS requires membrane, scaffolding, and cytoskeletal proteins, including Ankyrin-G and TRIM46. How these components cooperate in AIS formation is currently poorly understood. Here, we show that Ankyrin-G acts as a scaffold interacting with End-Binding (EB) proteins and membrane proteins such as Neurofascin-186 to recruit TRIM46-positive microtubules to the plasma membrane. Using in vitro reconstitution and cellular assays, we demonstrate that TRIM46 forms parallel microtubule bundles and stabilizes them by acting as a rescue factor. TRIM46-labeled microtubules drive retrograde transport of Neurofascin-186 to the proximal axon, where Ankyrin-G prevents its endocytosis, resulting in stable accumulation of Neurofascin-186 at the AIS. Neurofascin-186 enrichment in turn reinforces membrane anchoring of Ankyrin-G and subsequent recruitment of TRIM46-decorated microtubules. Our study reveals feedback-based mechanisms driving AIS assembly.

Published
2019

6. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity

Author

Farías, Ginny G., Fréal, Amélie, Tortosa, Elena, Stucchi, Riccardo, Pan, Xingxiu, Portegies, Sybren, Will, Lena, Altelaar, Maarten, Hoogenraad, Casper C., Farías, Ginny G., Fréal, Amélie, Tortosa, Elena, Stucchi, Riccardo, Pan, Xingxiu, Portegies, Sybren, Will, Lena, Altelaar, Maarten, and Hoogenraad, Casper C.

Abstract

Establishment of neuronal polarity depends on local microtubule (MT) reorganization. The endoplasmic reticulum (ER) consists of cisternae and tubules and, like MTs, forms an extensive network throughout the entire cell. How the two networks interact and control neuronal development is an outstanding question. Here we show that the interplay between MTs and the ER is essential for neuronal polarity. ER tubules localize within the axon, whereas ER cisternae are retained in the somatodendritic domain. MTs are essential for axonal ER tubule stabilization, and, reciprocally, the ER is required for stabilizing and organizing axonal MTs. Recruitment of ER tubules into one minor neurite initiates axon formation, whereas ER retention in the perinuclear area or disruption of ER tubules prevent neuronal polarization. The ER-shaping protein P180, present in axonal ER tubules, controls axon specification by regulating local MT remodeling. We propose a model in which feedback-driven regulation between the ER and MTs instructs neuronal polarity. Farías et al. report that the localization of the endoplasmic reticulum (ER) in the axon is controlled by the interaction between ER-shaping proteins and the microtubule cytoskeleton. Local ER and microtubule crosstalk promotes ER tubule-microtubule stabilization and drives neuronal polarity.

Published
2019

7. Feedback-Driven Assembly of the Axon Initial Segment

Author

Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, Celbiologie, Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, Hoogenraad, Casper C, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, Celbiologie, Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, and Hoogenraad, Casper C

Published
2019

8. TRIM46 Organizes Microtubule Fasciculation in the Axon Initial Segment

Author

Sub Cell Biology, Sub Cryo - EM, Celbiologie, Cryo-EM, Harterink, Martin, Vocking, Karin, Pan, Xingxiu, Soriano Jerez, Eva M., Slenders, Lotte, Fréal, Amélie, Tas, Roderick P., Van De Wetering, Willine J., Timmer, Karina, Motshagen, Jasmijn, Van Beuningen, Sam F.b., Kapitein, Lukas C., Geerts, Willie J.c., Post, Jan A., Hoogenraad, Casper C., Sub Cell Biology, Sub Cryo - EM, Celbiologie, Cryo-EM, Harterink, Martin, Vocking, Karin, Pan, Xingxiu, Soriano Jerez, Eva M., Slenders, Lotte, Fréal, Amélie, Tas, Roderick P., Van De Wetering, Willine J., Timmer, Karina, Motshagen, Jasmijn, Van Beuningen, Sam F.b., Kapitein, Lukas C., Geerts, Willie J.c., Post, Jan A., and Hoogenraad, Casper C.

Published
2019

9. Feedback-Driven Assembly of the Axon Initial Segment

Author

Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, Hoogenraad, Casper C, Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, and Hoogenraad, Casper C

Abstract

The axon initial segment (AIS) is a unique neuronal compartment that plays a crucial role in the generation of action potential and neuronal polarity. The assembly of the AIS requires membrane, scaffolding, and cytoskeletal proteins, including Ankyrin-G and TRIM46. How these components cooperate in AIS formation is currently poorly understood. Here, we show that Ankyrin-G acts as a scaffold interacting with End-Binding (EB) proteins and membrane proteins such as Neurofascin-186 to recruit TRIM46-positive microtubules to the plasma membrane. Using in vitro reconstitution and cellular assays, we demonstrate that TRIM46 forms parallel microtubule bundles and stabilizes them by acting as a rescue factor. TRIM46-labeled microtubules drive retrograde transport of Neurofascin-186 to the proximal axon, where Ankyrin-G prevents its endocytosis, resulting in stable accumulation of Neurofascin-186 at the AIS. Neurofascin-186 enrichment in turn reinforces membrane anchoring of Ankyrin-G and subsequent recruitment of TRIM46-decorated microtubules. Our study reveals feedback-based mechanisms driving AIS assembly.

Published
2019

10. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity

Author

Celbiologie, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, Farías, Ginny G., Fréal, Amélie, Tortosa, Elena, Stucchi, Riccardo, Pan, Xingxiu, Portegies, Sybren, Will, Lena, Altelaar, Maarten, Hoogenraad, Casper C., Celbiologie, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, Farías, Ginny G., Fréal, Amélie, Tortosa, Elena, Stucchi, Riccardo, Pan, Xingxiu, Portegies, Sybren, Will, Lena, Altelaar, Maarten, and Hoogenraad, Casper C.

Published
2019

11. Feedback-Driven Assembly of the Axon Initial Segment

Author

Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, Celbiologie, Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, Hoogenraad, Casper C, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, Celbiologie, Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, and Hoogenraad, Casper C

Published
2019

12. TRIM46 Organizes Microtubule Fasciculation in the Axon Initial Segment

Author

Sub Cell Biology, Sub Cryo - EM, Celbiologie, Cryo-EM, Harterink, Martin, Vocking, Karin, Pan, Xingxiu, Soriano Jerez, Eva M., Slenders, Lotte, Fréal, Amélie, Tas, Roderick P., Van De Wetering, Willine J., Timmer, Karina, Motshagen, Jasmijn, Van Beuningen, Sam F.b., Kapitein, Lukas C., Geerts, Willie J.c., Post, Jan A., Hoogenraad, Casper C., Sub Cell Biology, Sub Cryo - EM, Celbiologie, Cryo-EM, Harterink, Martin, Vocking, Karin, Pan, Xingxiu, Soriano Jerez, Eva M., Slenders, Lotte, Fréal, Amélie, Tas, Roderick P., Van De Wetering, Willine J., Timmer, Karina, Motshagen, Jasmijn, Van Beuningen, Sam F.b., Kapitein, Lukas C., Geerts, Willie J.c., Post, Jan A., and Hoogenraad, Casper C.

Published
2019

13. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity

Author

Celbiologie, Biomolecular Mass Spectrometry and Proteomics, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Farías, Ginny G., Fréal, Amélie, Tortosa, Elena, Stucchi, Riccardo, Pan, Xingxiu, Portegies, Sybren, Will, Lena, Altelaar, Maarten, Hoogenraad, Casper C., Celbiologie, Biomolecular Mass Spectrometry and Proteomics, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Farías, Ginny G., Fréal, Amélie, Tortosa, Elena, Stucchi, Riccardo, Pan, Xingxiu, Portegies, Sybren, Will, Lena, Altelaar, Maarten, and Hoogenraad, Casper C.

Published
2019

14. Feedback-Driven Assembly of the Axon Initial Segment

Author

Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, Cell Biology, Neurobiology and Biophysics, Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, Hoogenraad, Casper C, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Biomolecular Mass Spectrometry and Proteomics, Cell Biology, Neurobiology and Biophysics, Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, and Hoogenraad, Casper C

Published
2019

15. TRIM46 Organizes Microtubule Fasciculation in the Axon Initial Segment

Author

Sub Cell Biology, Sub Cryo - EM, Cell Biology, Neurobiology and Biophysics, Cryo-EM, Harterink, Martin, Vocking, Karin, Pan, Xingxiu, Soriano Jerez, Eva M., Slenders, Lotte, Fréal, Amélie, Tas, Roderick P., Van De Wetering, Willine J., Timmer, Karina, Motshagen, Jasmijn, Van Beuningen, Sam F.b., Kapitein, Lukas C., Geerts, Willie J.c., Post, Jan A., Hoogenraad, Casper C., Sub Cell Biology, Sub Cryo - EM, Cell Biology, Neurobiology and Biophysics, Cryo-EM, Harterink, Martin, Vocking, Karin, Pan, Xingxiu, Soriano Jerez, Eva M., Slenders, Lotte, Fréal, Amélie, Tas, Roderick P., Van De Wetering, Willine J., Timmer, Karina, Motshagen, Jasmijn, Van Beuningen, Sam F.b., Kapitein, Lukas C., Geerts, Willie J.c., Post, Jan A., and Hoogenraad, Casper C.

Published
2019

16. Feedback-Driven Mechanisms between Microtubules and the Endoplasmic Reticulum Instruct Neuronal Polarity

Author

Cell Biology, Neurobiology and Biophysics, Biomolecular Mass Spectrometry and Proteomics, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Farías, Ginny G., Fréal, Amélie, Tortosa, Elena, Stucchi, Riccardo, Pan, Xingxiu, Portegies, Sybren, Will, Lena, Altelaar, Maarten, Hoogenraad, Casper C., Cell Biology, Neurobiology and Biophysics, Biomolecular Mass Spectrometry and Proteomics, Sub Cell Biology, Afd Biomol.Mass Spect. and Proteomics, Farías, Ginny G., Fréal, Amélie, Tortosa, Elena, Stucchi, Riccardo, Pan, Xingxiu, Portegies, Sybren, Will, Lena, Altelaar, Maarten, and Hoogenraad, Casper C.

Published
2019

17. Feedback-Driven Assembly of the Axon Initial Segment

Author

Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, Hoogenraad, Casper C, Fréal, Amélie, Rai, Dipti, Tas, Roderick P, Pan, Xingxiu, Katrukha, Eugene A, van de Willige, Dieudonnée, Stucchi, Riccardo, Aher, Amol, Yang, Chao, Altelaar, A F Maarten, Vocking, Karin, Post, Jan Andries, Harterink, Martin, Kapitein, Lukas C, Akhmanova, Anna, and Hoogenraad, Casper C

Abstract

The axon initial segment (AIS) is a unique neuronal compartment that plays a crucial role in the generation of action potential and neuronal polarity. The assembly of the AIS requires membrane, scaffolding, and cytoskeletal proteins, including Ankyrin-G and TRIM46. How these components cooperate in AIS formation is currently poorly understood. Here, we show that Ankyrin-G acts as a scaffold interacting with End-Binding (EB) proteins and membrane proteins such as Neurofascin-186 to recruit TRIM46-positive microtubules to the plasma membrane. Using in vitro reconstitution and cellular assays, we demonstrate that TRIM46 forms parallel microtubule bundles and stabilizes them by acting as a rescue factor. TRIM46-labeled microtubules drive retrograde transport of Neurofascin-186 to the proximal axon, where Ankyrin-G prevents its endocytosis, resulting in stable accumulation of Neurofascin-186 at the AIS. Neurofascin-186 enrichment in turn reinforces membrane anchoring of Ankyrin-G and subsequent recruitment of TRIM46-decorated microtubules. Our study reveals feedback-based mechanisms driving AIS assembly.

Published
2019

18. Cooperative Interactions between 480 kDa Ankyrin-G and EB proteins assemble the axon initial segment

Author

Fréal, Amélie, Fassier, Coralie, Le Bras, Barbara, Bullier, Erika, de Gois, Stéphanie, Hazan, Jamilé, Hoogenraad, Casper C., Couraud, François, Fréal, Amélie, Fassier, Coralie, Le Bras, Barbara, Bullier, Erika, de Gois, Stéphanie, Hazan, Jamilé, Hoogenraad, Casper C., and Couraud, François

Abstract

The axon initial segment (AIS) is required for generating action potentials and maintaining neuronal polarity. Significant progress has been made in deciphering the basic building blocks composing the AIS, but the underlying mechanisms required for AIS formation remains unclear. The scaffolding protein ankyrin-G is the master-organizer of the AIS. Microtubules and their interactors, particularly end-binding proteins (EBs), have emerged as potential key players in AIS formation. Here, we show that the longest isoform of ankyrin-G (480AnkG) selectively associates with EBs via its specific tail domain and that this interaction is crucial for AIS formation and neuronal polarity in cultured rodent hippocampal neurons. EBs are essential for 480AnkG localization and stabilization at the AIS, whereas 480AnkG is required for the specific accumulation of EBs in the proximal axon. Our findings thus provide a conceptual framework for understanding how the cooperative relationship between 480AnkG and EBs induces the assembly of microtubule-AIS structures in the proximal axon. Significance Statement Neuronal polarity is crucial for the proper function of neurons. The assembly of the axon initial segment (AIS), which is the hallmark of early neuronal polarization, relies on the longest 480 kDa ankyrin-G isoform. The microtubule cytoskeleton and its interacting proteins were suggested to be early key players in the process of AIS formation. In this study, we show that the crosstalk between 480 kDa ankyrin-G and the microtubule plus-end tracking proteins, EBs, at the proximal axon is decisive for AIS assembly and neuronal polarity. Our work thus provides insight into the functional mechanisms used by 480 kDa ankyrin-G to drive the AIS formation and thereby to establish neuronal polarity.

Published
2016

19. Cooperative Interactions between 480 kDa Ankyrin-G and EB proteins assemble the axon initial segment

Author

Celbiologie, Sub Cell Biology, Fréal, Amélie, Fassier, Coralie, Le Bras, Barbara, Bullier, Erika, de Gois, Stéphanie, Hazan, Jamilé, Hoogenraad, Casper C., Couraud, François, Celbiologie, Sub Cell Biology, Fréal, Amélie, Fassier, Coralie, Le Bras, Barbara, Bullier, Erika, de Gois, Stéphanie, Hazan, Jamilé, Hoogenraad, Casper C., and Couraud, François

Published
2016

20. Cooperative Interactions between 480 kDa Ankyrin-G and EB proteins assemble the axon initial segment

Author

Celbiologie, Sub Cell Biology, Fréal, Amélie, Fassier, Coralie, Le Bras, Barbara, Bullier, Erika, de Gois, Stéphanie, Hazan, Jamilé, Hoogenraad, Casper C., Couraud, François, Celbiologie, Sub Cell Biology, Fréal, Amélie, Fassier, Coralie, Le Bras, Barbara, Bullier, Erika, de Gois, Stéphanie, Hazan, Jamilé, Hoogenraad, Casper C., and Couraud, François

Published
2016

21. Cooperative Interactions between 480 kDa Ankyrin-G and EB proteins assemble the axon initial segment

Author

Cell Biology, Neurobiology and Biophysics, Sub Cell Biology, Fréal, Amélie, Fassier, Coralie, Le Bras, Barbara, Bullier, Erika, de Gois, Stéphanie, Hazan, Jamilé, Hoogenraad, Casper C., Couraud, François, Cell Biology, Neurobiology and Biophysics, Sub Cell Biology, Fréal, Amélie, Fassier, Coralie, Le Bras, Barbara, Bullier, Erika, de Gois, Stéphanie, Hazan, Jamilé, Hoogenraad, Casper C., and Couraud, François

Published
2016

22. Le Nord / texte de Jacques Fréal ; illustrations de Mathieu Ravaux

Author

Ravaux, Mathieu. Illustrateur, Fréal, Jacques. Auteur du texte, Ravaux, Mathieu. Illustrateur, and Fréal, Jacques. Auteur du texte

Abstract

Collection : Témoins de la vie paysanne ; 1, Collection : Témoins de la vie paysanne ; 1, Contient une table des matières, Avec mode texte, Catalogues d'exposition

Published
1980

25. Le Nord / texte de Jacques Fréal ; illustrations de Mathieu Ravaux

Author

Ravaux, Mathieu. Illustrateur, Fréal, Jacques. Auteur du texte, Ravaux, Mathieu. Illustrateur, and Fréal, Jacques. Auteur du texte

Abstract

Collection : Témoins de la vie paysanne ; 1, Collection : Témoins de la vie paysanne ; 1, Contient une table des matières, Avec mode texte, Catalogues d'exposition

Published
1980

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