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5 results on '"Illanes, Ramiro"'

1. Plastic degradation by insect hexamerins: Near-atomic resolution structures of the polyethylene-degrading proteins from the wax worm saliva

Author

Röchling Foundation, Consejo Superior de Investigaciones Científicas (España), Ministerio de Ciencia e Innovación (España), Generalitat de Catalunya, Ministerio de Ciencia, Innovación y Universidades (España), Spínola-Amilibia, Mercedes [0000-0002-0641-8809], Illanes, Ramiro [0000-0002-1710-061X], Ruiz-López, Elena [0000-0002-3922-2001], Colomer-Vidal, Pere [0000-0002-5911-8287], Peces-Pérez, Rosa [0000-0003-2344-1549], Arias, Clemente F. [0000-0003-4298-8910], Torroba, Tomás [0000-0002-5018-4173], Solà, Maria [0000-0002-0838-9706], Arias-Palomo, Ernesto [0000-0002-2706-7411], Bertocchini, Federica [0000-0001-7241-0648], Spínola-Amilibia, Mercedes, Illanes, Ramiro, Ruiz-López, Elena, Colomer-Vidal, Pere, Rodríguez-Ventura, F., Peces-Pérez, Rosa, Arias, Clemente F., Torroba, Tomás, Solà, Maria, Arias-Palomo, Ernesto, Bertocchini, Federica, Röchling Foundation, Consejo Superior de Investigaciones Científicas (España), Ministerio de Ciencia e Innovación (España), Generalitat de Catalunya, Ministerio de Ciencia, Innovación y Universidades (España), Spínola-Amilibia, Mercedes [0000-0002-0641-8809], Illanes, Ramiro [0000-0002-1710-061X], Ruiz-López, Elena [0000-0002-3922-2001], Colomer-Vidal, Pere [0000-0002-5911-8287], Peces-Pérez, Rosa [0000-0003-2344-1549], Arias, Clemente F. [0000-0003-4298-8910], Torroba, Tomás [0000-0002-5018-4173], Solà, Maria [0000-0002-0838-9706], Arias-Palomo, Ernesto [0000-0002-2706-7411], Bertocchini, Federica [0000-0001-7241-0648], Spínola-Amilibia, Mercedes, Illanes, Ramiro, Ruiz-López, Elena, Colomer-Vidal, Pere, Rodríguez-Ventura, F., Peces-Pérez, Rosa, Arias, Clemente F., Torroba, Tomás, Solà, Maria, Arias-Palomo, Ernesto, and Bertocchini, Federica

Abstract

Plastic waste management is a pressing ecological, social, and economic challenge. The saliva of the lepidopteran Galleria mellonella larvae is capable of oxidizing and depolymerizing polyethylene in hours at room temperature. Here, we analyze by cryo–electron microscopy (cryo-EM) G. mellonella’s saliva directly from the native source. The three-dimensional reconstructions reveal that the buccal secretion is mainly composed of four hexamerins belonging to the hemocyanin/phenoloxidase family, renamed Demetra, Cibeles, Ceres, and a previously unidentified factor termed Cora. Functional assays show that this factor, as its counterparts Demetra and Ceres, is also able to oxidize and degrade polyethylene. The cryo-EM data and the x-ray analysis from purified fractions show that they self-assemble primarily into three macromolecular complexes with striking structural differences that likely modulate their activity. Overall, these results establish the ground to further explore the hexamerins’ functionalities, their role in vivo, and their eventual biotechnological application.

Published
2023

2. Wax worm saliva and the enzymes therein are the key to polyethylene degradation by Galleria mellonella

Author

Röchling Foundation, Consejo Superior de Investigaciones Científicas (España), North Atlantic Treaty Organization, Junta de Castilla y León, Ministerio de Ciencia e Innovación (España), Generalitat de Catalunya, Colomer-Vidal, Pere [0000-0002-5911-8287], Spínola-Amilibia, Mercedes [0000-0002-0641-8809], Ruiz-López, Elena [0000-0002-3922-2001], Illanes, Ramiro [0000-0002-1710-061X], Aiese Cigliano, Riccardo [0000-0002-9058-6994], Montoya, María [0000-0002-5703-7360], Falabella, P. [0000-0003-0304-6867], Pesquera, Carmen [0000-0002-8635-0380], González-Legarreta, L. [0000-0001-5193-7256], Arias-Palomo, Ernesto [0000-0002-2706-7411], Solà, Maria [0000-0002-0838-9706], Torroba, Tomás [0000-0002-5018-4173], Arias, Clemente F [0000-0003-4298-8910], Bertocchini, Federica [0000-0001-7241-0648], Sanluis-Verdes, A., Colomer-Vidal, Pere, Rodríguez-Ventura, F., Bello-Villarino, M., Spínola-Amilibia, Mercedes, Ruiz-López, Elena, Illanes, Ramiro, Castroviejo, P., Aiese Cigliano, Riccardo, Montoya, María, Falabella, P., Pesquera, Carmen, González-Legarreta, L., Arias-Palomo, Ernesto, Solà, Maria, Torroba, Tomás, Arias, Clemente F., Bertocchini, Federica, Röchling Foundation, Consejo Superior de Investigaciones Científicas (España), North Atlantic Treaty Organization, Junta de Castilla y León, Ministerio de Ciencia e Innovación (España), Generalitat de Catalunya, Colomer-Vidal, Pere [0000-0002-5911-8287], Spínola-Amilibia, Mercedes [0000-0002-0641-8809], Ruiz-López, Elena [0000-0002-3922-2001], Illanes, Ramiro [0000-0002-1710-061X], Aiese Cigliano, Riccardo [0000-0002-9058-6994], Montoya, María [0000-0002-5703-7360], Falabella, P. [0000-0003-0304-6867], Pesquera, Carmen [0000-0002-8635-0380], González-Legarreta, L. [0000-0001-5193-7256], Arias-Palomo, Ernesto [0000-0002-2706-7411], Solà, Maria [0000-0002-0838-9706], Torroba, Tomás [0000-0002-5018-4173], Arias, Clemente F [0000-0003-4298-8910], Bertocchini, Federica [0000-0001-7241-0648], Sanluis-Verdes, A., Colomer-Vidal, Pere, Rodríguez-Ventura, F., Bello-Villarino, M., Spínola-Amilibia, Mercedes, Ruiz-López, Elena, Illanes, Ramiro, Castroviejo, P., Aiese Cigliano, Riccardo, Montoya, María, Falabella, P., Pesquera, Carmen, González-Legarreta, L., Arias-Palomo, Ernesto, Solà, Maria, Torroba, Tomás, Arias, Clemente F., and Bertocchini, Federica

Abstract

Plastic degradation by biological systems with re-utilization of the by-products can be the future solution to the global threat of plastic waste accumulation. We report that the saliva of Galleria mellonella larvae (wax worms) is capable of oxidizing and depolymerizing polyethylene (PE), one of the most produced and sturdy polyolefin-derived plastics. This effect is achieved after a few hours’ exposure at room temperature and physiological conditions (neutral pH). The wax worm saliva can indeed overcome the bottleneck step in PE biodegradation, that is the initial oxidation step. Within the saliva, we identified two enzymes that can reproduce the same effect. This is the first report of enzymes with this capability, opening up the way to new ground-breaking solutions for plastic waste management through bio-recycling/up-cycling.

Published
2022

3. Supplementary files of the article Wax worm saliva and the enzymes therein are the key to polyethylene degradation by Galleria mellonella [Dataset]

Author

Sanluis-Verdes, A., Colomer-Vidal, Pere, Rodríguez-Ventura, F., Bello-Villarino, M., Spínola-Amilibia, Mercedes, Ruiz-López, Elena, Illanes, Ramiro, Castroviejo, P., Aiese Cigliano, Riccardo, Montoya, María, Falabella, P., Pesquera, Carmen, González-Legarreta, L., Arias-Palomo, Ernesto, Sola, María, Torroba, Tomás, Arias, Clemente F., Bertocchini, Federica, Sanluis-Verdes, A., Colomer-Vidal, Pere, Rodríguez-Ventura, F., Bello-Villarino, M., Spínola-Amilibia, Mercedes, Ruiz-López, Elena, Illanes, Ramiro, Castroviejo, P., Aiese Cigliano, Riccardo, Montoya, María, Falabella, P., Pesquera, Carmen, González-Legarreta, L., Arias-Palomo, Ernesto, Sola, María, Torroba, Tomás, Arias, Clemente F., and Bertocchini, Federica

Published
2022

5. Immunodominant proteins P1 and P40/P90 from human pathogen Mycoplasma pneumoniae

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Instituto de Salud Carlos III, Osaka University, Vizarraga, David, Kawamoto, Akihiro, Matsumoto, U., Illanes, Ramiro, Pérez-Luque, Rosa, Martín, J., Mazzolini, Rocco, Bierge, Paula, Pich, Oscar Q., Espasa, Mateu, Sanfeliu, Isabel, Esperalba, Juliana, Fernández-Huerta, Miguel, Scheffer, Margot P., Pinyol, Jaume, Frangakis, Achilleas S., Lluch-Senar, María, Mori, Shigetarou, Shibayama, Keigo, Kenri, Tsuyoshi, Kato, Takayuki, Namba, Keiichi, Fita, Ignacio, Miyata, Makoto, Aparicio, David, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Instituto de Salud Carlos III, Osaka University, Vizarraga, David, Kawamoto, Akihiro, Matsumoto, U., Illanes, Ramiro, Pérez-Luque, Rosa, Martín, J., Mazzolini, Rocco, Bierge, Paula, Pich, Oscar Q., Espasa, Mateu, Sanfeliu, Isabel, Esperalba, Juliana, Fernández-Huerta, Miguel, Scheffer, Margot P., Pinyol, Jaume, Frangakis, Achilleas S., Lluch-Senar, María, Mori, Shigetarou, Shibayama, Keigo, Kenri, Tsuyoshi, Kato, Takayuki, Namba, Keiichi, Fita, Ignacio, Miyata, Makoto, and Aparicio, David

Abstract

Mycoplasma pneumoniae is a bacterial human pathogen that causes primary atypical pneumonia. M. pneumoniae motility and infectivity are mediated by the immunodominant proteins P1 and P40/P90, which form a transmembrane adhesion complex. Here we report the structure of P1, determined by X-ray crystallography and cryo-electron microscopy, and the X-ray structure of P40/P90. Contrary to what had been suggested, the binding site for sialic acid was found in P40/P90 and not in P1. Genetic and clinical variability concentrates on the N-terminal domain surfaces of P1 and P40/P90. Polyclonal antibodies generated against the mostly conserved C-terminal domain of P1 inhibited adhesion of M. pneumoniae, and serology assays with sera from infected patients were positive when tested against this C-terminal domain. P40/P90 also showed strong reactivity against human infected sera. The architectural elements determined for P1 and P40/P90 open new possibilities in vaccine development against M. pneumoniae infections.

Published
2020

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