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Dan Li,1,&ast; Xin Zhang,2,3,&ast; Yunzhong Wang,2 Jian Xue,2 Xueqiang Ji,2 Xuejun Shao,2– 4 Yang Li2– 4 1Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215004, People’s Republic of China; 2Department of Clinical Laboratory, Children’s Hospital of Soochow University, Suzhou, Jiangsu, 215025, People’s Republic of China; 3Institute of Pediatric Research, Children’s Hospital of Soochow University, Suzhou, Jiangsu, 215025, People’s Republic of China; 4Clinical Medical College of Pediatrics, Soochow University, Suzhou, Jiangsu, 215025, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Yang Li; Xuejun Shao Email 11418092@zju.edu.cn; xjshao@suda.edu.cnObjective: To investigate the epidemiology and drug resistance of pathogens isolated from cerebrospinal fluid samples at a children’s medical center in eastern China and provide the basis for anti-infection treatments.Methods: In all, 307 non-duplicated strains of pathogens were isolated from cerebrospinal fluid samples in the Children’s Hospital of Soochow University from January 2006 to December 2020. Mass spectrometry was used for pathogen identification. The VITEK 2 Compact system and Kirby-Bauer method were applied to determine antimicrobial susceptibility.Results: Among the 307 isolates, gram-positive bacteria, gram-negative bacteria and fungi accounted for 60.26%, 34.53%, and 5.21%, respectively. The most prevalent pathogens were Streptococcus pneumoniae (26.06%), Escherichia coli (20.20%) and Streptococcus agalactiae (17.26%). The number of isolates was highest in winter. The most prevalent gram-positive bacterium in children < 6 months old was Streptococcus agalactiae, while Streptococcus pneumoniae was the most in children were > 6 months old. The drug resistance of gram-positive bacteria, fungi and Haemophilus influenza were not high. In addition, 35 strains of gram-negative ba

Yang Li,1– 4,&ast; Dan Li,5,&ast; Jian Xue,4 Xueqiang Ji,4 Xuejun Shao,4 Jie Yan1– 3 1Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003, People’s Republic of China; 2Department of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310058, People’s Republic of China; 3Division of Basic Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang, 310003, People’s Republic of China; 4Department of Clinical Laboratory, Children’s Hospital of Soochow University, Suzhou, Jiangsu, 215025, People’s Republic of China; 5Department of Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou, Jiangsu, 215004, People’s Republic of China&ast;These authors contributed equally to this workCorrespondence: Jie YanDepartment of Medical Microbiology and Parasitology, Zhejiang University School of Medicine, 866 Yu-Hang-Tang Road, Hangzhou, Zhejiang, 310058, People’s Republic of ChinaTel +86-571-88208297Fax +86-571-88208294Email Med_bp@zju.edu.cnXuejun ShaoDepartment of Clinical Laboratory, Children’s Hospital of Soochow University, 92 Zhong-Nan Street, Suzhou, Jiangsu, 215025, People’s Republic of ChinaTel +86-512-80691203Email xjshao@suda.edu.cnObjective: This study investigated the epidemiology, virulence and drug resistance of invasive Klebsiella pneumoniae (K. pneumoniae) isolates at a children’s medical center in eastern China in order to obtain epidemiologic, virulence, and antimicrobial resistance data that can guide for the selection and development of anti-infection treatments.Methods: A total of 94 invasive K. pneumoniae strains were isolated from children between January 2016 and December 2020 at the Children’s Hospital of Soochow University. Th