Search

1. Crude homogenates from Neurospora crassa grown on varions concentrations of sucrose and acetate were assayed for enzymes regulating isocitrate breakdown.

Streptococcus thermophilus is one of the main economically important lactic acid bacteria used in the dairy industry. Increasing knowledge of its metabolic properties and physiology is a prerequisite for the improvement of its industrial properties. Determination of the entire genome sequence of S. thermophilus LMG18311 was used in this study to investigate the presence of two-component systems (TCSs) and their respective functions. Attention was especially focussed on TCSs possibly involved in the regulation of the production of antimicrobial compounds such as bacteriocins. Among the ten TCSs identified, two of them showed homology with TCSs related to bacteriocin biosynthesis loci. The aim of the present study was to characterize one of the gene clusters containing a TCS showing strong homology with the previously identified blp gene cluster (bacteriocin like peptide) of Streptococcus pneumoniae. Such a gene cluster was also present in the two related S. thermophilus strains, LMD9 and CNRZ1066, for which the full genome sequence is also available. However, some differences were present when comparing the organization of the blp gene clusters of the three strains. That allowed us to look for a functional cluster and to characterize the antimicrobial activity related to those dedicated gene clusters. The comparison of those gene clusters together with the identification of the bacteriocin encoding genes will be presented.

Streptococcus thermophilus is one of the main economically important lactic acid bacteria used in the dairy industry. Increasing knowledge of its metabolic properties and physiology is a prerequisite for the improvement of its industrial properties. Determination of the entire genome sequence of S. thermophilus LMG18311 was used in this study to investigate the presence of two-component systems (TCSs) and their respective functions. Attention was especially focussed on TCSs possibly involved in the regulation of the production of antimicrobial compounds such as bacteriocins. Among the ten TCSs identified, two of them showed homology with TCSs related to bacteriocin biosynthesis loci. The aim of the present study was to characterize one of the gene clusters containing a TCS showing strong homology with the previously identified blp gene cluster (bacteriocin like peptide) of Streptococcus pneumoniae. Such a gene cluster was also present in the two related S. thermophilus strains, LMD9 and CNRZ1066, for which the full genome sequence is also available. However, some differences were present when comparing the organization of the blp gene clusters of the three strains. That allowed us to look for a functional cluster and to characterize the antimicrobial activity related to those dedicated gene clusters. The comparison of those gene clusters together with the identification of the bacteriocin encoding genes will be presented.

info:eu-repo/semantics/nonPublished