Search

Na Qi,1,* Xiantai Zhou,2,* Ningzhu Ma,2 Jianguo Zhang,2 Zhenlin Wang,2 Xin Zhang,1 Aimin Li1 1Cancer Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510315, People’s Republic of China; 2Department of Pharmacy, Guilin Medical University, Guilin, 541004, People’s Republic of China*These authors contributed equally to this workCorrespondence: Aimin Li; Xin Zhang, Cancer Center, Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou, 510315, People’s Republic of China, Email liaimin2005@163.com; 252089553@qq.comIntroduction: The high mortality rate of malignant ovarian cancer is attributed to the absence of effective early diagnosis methods. The LHRH receptor is specifically overexpressed in most ovarian cancers, and the integrin αvβ 3 receptor is also overexpressed on the surface of ovarian cancer cells. In this study, we designed LHRH analogues (LHRHa)/RGD co-modified paclitaxel liposomes (LHRHa-RGD-LP-PTX) to target LHRH receptor-positive ovarian cancers more effectively and enhance the anti-ovarian cancer effects.Methods: LHRHa-RGD-LP-PTX liposomes were prepared using the thin film hydration method. The morphology, physicochemical properties, cellular uptake, and cell viability were assessed. Additionally, the cellular uptake mechanism of the modified liposomes was investigated using various endocytic inhibitors. The inhibitory effect of the formulations on tumor spheroids was observed under a microscope. The co-localization with lysosomes was visualized using confocal laser scanning microscopy (CLSM), and the in vivo tumor-targeting ability of the formulations was assessed using the IVIS fluorescent imaging system. Finally, the in vivo anti-tumor efficacy of the formulations was evaluated in the armpits of BALB/c nude mice.Results: The results indicated that LHRHa-RGD-LP-PTX significantly enhanced cellular uptake in A2780 cells, increased cytoto

In this study, fcSe@TiO2 and [Cu2I2(fcSe)2]n@TiO2 nanosystems based on ferrocenylselenoether and its cuprous cluster were developed and characterized by X-ray photoelectron spectroscopy (XPS), high-resolution transmission electron microscopy (HR-TEM), energy dispersive X-ray spectroscopy (EDX), and electron paramagnetic resonance (EPR). Under optimized conditions, 0.2 g L−1 catalyst, 20 mM H2O2, and initial pH 7, good synergistic visible light photocatalytic tetracycline degradation and Cr(vi) reduction were achieved, with 92.1% of tetracycline and 64.5% of Cr(vi) removal efficiency within 30 minutes. Mechanistic studies revealed that the reactive species ˙OH, ˙O2−, and h+ were produced in both systems through the mutual promotion of Fenton reactions and photogenerated charge separation. The [Cu2I2(fcSe)2]n@TiO2 system additionally produced 1O2 from Cu+ and ˙O2−. The advantages of the developed nanosystems include an acidic surface microenvironment provided by Se⋯H+, resourceful product formation, tolerance of complex environments, and excellent adaptability in refractory N-cyclic organics.

In this paper, we study a remote monitoring system where a receiver observes a remote binary Markov source and decides whether to sample and transmit the state through a randomly delayed channel. We adopt uncertainty of information (UoI), defined as the entropy conditional on past observations at the receiver, as a metric of value of information, in contrast to the traditional state-agnostic nonlinear age of information (AoI) penalty functions. To address the limitations of prior UoI research that assumes one-time-slot delays, we extend our analysis to scenarios with random delays. We model the problem as a partially observable Markov decision process (POMDP) problem and simplify it to a semi-Markov decision process (SMDP) by introducing the belief state. We propose two algorithms: A globally optimal bisection relative value iteration (bisec-RVI) algorithm and a computationally efficient sub-optimal index-based threshold algorithm to solve the long-term average UoI minimization problem. Numerical simulations demonstrate that our sampling policies surpass traditional zero wait and AoI-optimal policies, particularly under conditions of large delay, with the sub-optimal policy nearly matching the performance of the optimal one.

Age of Information (AoI) has been recognized as an important metric to measure the freshness of information. Central to this consensus is that minimizing AoI can enhance the freshness of information, thereby facilitating the accuracy of subsequent decision-making processes. However, to date the direct causal relationship that links AoI to the utility of the decision-making process is unexplored. To fill this gap, this paper provides a sampling-control co-design problem, referred to as an age-aware remote Markov Decision Process (MDP) problem, to explore this unexplored relationship. Our framework revisits the sampling problem in [1] with a refined focus: moving from AoI penalty minimization to directly optimizing goal-oriented remote decision-making process under random delay. We derive that the age-aware remote MDP problem can be reduced to a standard MDP problem without delays, and reveal that treating AoI solely as a metric for optimization is not optimal in achieving remote decision making. Instead, AoI can serve as important side information to facilitate remote decision making., Comment: 12 pages, 4 figures

The intricate interplay of source dynamics, unreliable channels, and staleness of information has long been recognized as a significant impediment for the receiver to achieve accurate, timely, and most importantly, goal-oriented decision making. Thus, a plethora of promising metrics, such as Age of Information, Value of Information, and Mean Square Error, have emerged to quantify these underlying adverse factors. Following this avenue, optimizing these metrics has indirectly improved the utility of goal-oriented decision making. Nevertheless, no metric has hitherto been expressly devised to evaluate the utility of a goal-oriented decision-making process. To this end, this paper investigates a novel performance metric, the Goal-oriented Tensor (GoT), to directly quantify the impact of semantic mismatches on the goal-oriented decision making. Based on the GoT, we consider a sampler-decision maker pair that work collaboratively and distributively to achieve a shared goal of communications. We formulate an infinite-horizon Decentralized Partially Observable Markov Decision Process (Dec-POMDP) to conjointly deduce the optimal deterministic sampling policy and decision-making policy. The simulation results reveal that the sampler-decision maker co-design surpasses the current literature on AoI and its variants in terms of both goal achievement utility and sparse sampling rate, signifying a notable accomplishment for a sparse sampler and goal-oriented decision maker co-design., Comment: 6 pages, 4, figures, Submitted to 2023 Globecom

Spinal codes, a family of rateless codes introduced in 2011, have been proved to achieve Shannon capacity over both the additive white Gaussian noise (AWGN) channel and the binary symmetric channel (BSC). In this paper, we derive explicit tight upper bounds on the error probability of Spinal codes under maximum-likelihood (ML) decoding and perfect channel state information (CSI) over three typical fading channels, including the Rayleigh channel, the Nakagami-m channel and the Rician channel. Simulation results verify the tightness of the derived upper bounds.

The recent interweaving of AI-6G technologies has sparked extensive research interest in further enhancing reliable and timely communications. \emph{Age of Information} (AoI), as a novel and integrated metric implying the intricate trade-offs among reliability, latency, and update frequency, has been well-researched since its conception. This paper contributes new results in this area by employing a Deep Reinforcement Learning (DRL) approach to intelligently decide how to allocate power resources and when to retransmit in a \emph{freshness-sensitive} downlink multi-user Hybrid Automatic Repeat reQuest with Chase Combining (HARQ-CC) aided Non-Orthogonal Multiple Access (NOMA) network. Specifically, an AoI minimization problem is formulated as a Markov Decision Process (MDP) problem. Then, to achieve deterministic, age-optimal, and intelligent power allocations and retransmission decisions, the Double-Dueling-Deep Q Network (DQN) is adopted. Furthermore, a more flexible retransmission scheme, referred to as Retransmit-At-Will scheme, is proposed to further facilitate the timeliness of the HARQ-aided NOMA network. Simulation results verify the superiority of the proposed intelligent scheme and demonstrate the threshold structure of the retransmission policy. Also, answers to whether user pairing is necessary are discussed by extensive simulation results.

Since Shannon's pioneering masterpiece which established the prototype of modern information theory, the goal of communications has long been promising accurate message reconstruction at a refined bit-by-bit level, which deliberately neglects the semantics/effectiveness aspect of information. Nevertheless, the recent development of wireless technologies and the spurt of deep learning (DL) techniques allow us to reclaim the meaning/usefulness aspect in the design of future 6G communication paradigms, where goal-oriented communication is becoming a trend. Age of Information (AoI), a well-known metric that captures the importance of information by recording the time elapsed from the generation time slot, has been extended to various variants, such as Value of Information (VoI), Urgency of Information (UoI), Age of Incorrect Information (AoII), and etc. While each of them proposes novel ways to measure the meaning/usefulness aspect of information, there is not yet an integrated framework encompassing all of them. To this end, we propose a novel tensor-based approach, the Goal-oriented Tensor (GoT), to unify them, which also allows more flexible and fine-grained goal characterizations. Following the proposed GoT, we architect a holistic goal-oriented framework to enable goal-oriented semantic communications, in which information perception, dissemination, and control-plane decisions are implemented in accordance with specific goals. Finally, we outline several open challenges to fulfill the vision of the GoT framework.

Optimizations premised on open-loop metrics such as Age of Information (AoI) indirectly enhance the system's decision-making utility. We therefore propose a novel closed-loop metric named Goal-oriented Tensor (GoT) to directly quantify the impact of semantic mismatches on goal-oriented decision-making utility. Leveraging the GoT, we consider a sampler & decision-maker pair that works collaboratively and distributively to achieve a shared goal of communications. We formulate a two-agent infinite-horizon Decentralized Partially Observable Markov Decision Process (Dec-POMDP) to conjointly deduce the optimal deterministic sampling policy and decision-making policy. To circumvent the curse of dimensionality in obtaining an optimal deterministic joint policy through Brute-Force-Search, a sub-optimal yet computationally efficient algorithm is developed. This algorithm is predicated on the search for a Nash Equilibrium between the sampler and the decision-maker. Simulation results reveal that the proposed sampler & decision-maker co-design surpasses the current literature on AoI and its variants in terms of both goal achievement utility and sparse sampling rate, signifying progress in the semantics-conscious, goal-driven sparse sampling design., Comment: 30 pages, 9 figures. arXiv admin note: substantial text overlap with arXiv:2305.04083

Delivering timely status updates in a timeliness-critical communication system is of paramount importance to assist accurate and efficient decision making. Therefore, the topic of analyzing Age of Information has aroused new research interest. This paper contributes to new results in this area by systematically analyzing the AoI of two types of Hybrid Automatic Repeat reQuest (HARQ) techniques that have been newly standardized in the Release-16 5G New Radio (NR) specifications, namely reactive HARQ and proactive HARQ. Under a code-based status update system with non-trivial coding delay, transmission delay, propagation delay, decoding delay, and feedback delay, we derive unified closed-form average AoI and average Peak AoI expressions for reactive HARQ and proactive HARQ, respectively. Based on the obtained explicit expressions, we formulate an AoI minimization problem to investigate the age-optimal codeblock assignment strategy in the finite block-length (FBL) regime. Through case studies and analytical results, we provide comparative insights between reactive HARQ and proactive HARQ from a perspective of freshness of information. The numerical results and optimization solutions show that proactive HARQ draws its strength from both age performance and system robustness, thus enabling the potential to provide new system advancement of a freshness-critical status update system.

Spinal codes are a type of capacity-achieving rateless codes that have been proved to approach the Shannon capacity over the additive white Gaussian noise (AWGN) channel and the binary symmetric channel (BSC). In this paper, we aim to analyze the bounds on the error probability of Spinal codes and design a joint transmission-decoding system. First, in the finite block-length regime, we derive new upper bounds on the Maximum Likelihood (ML) decoding error probability for Spinal codes over both the AWGN channel and the BSC. Then, based on the derived bounds, we formulate a rate maximization problem. As the solution exhibits an incremental-tail-transmission pattern, we propose an improved transmission scheme, referred to as the thresholded incremental tail transmission (TITT) scheme. Moreover, we also develop a dynamic TITT-matching decoding algorithm, called the bubble decoding with memory (BD-M) algorithm, to reduce the decoding time complexity. The TITT scheme at the transmitter and the BD-M algorithm at the receiver jointly constitute a dynamic transmission-decoding system for Spinal code, improving its rate performance and decoding throughput. Theoretical analysis and simulation results are provided to verify the superiority of the derived bounds and the proposed joint transmission-decoding system design.

Yue Zhang,1 Jingyi Li,2 Chengzhang Liu,3 Hongxiang Yu,1 Chen Chen,1 Chonglei Bi,4 Chongqian Fang,4 Hai Ma,5 Aimin Li,6 Qing Dong,7 Lishun Liu,8 Binyan Wang,3 Xiao Huang,9 Xiaoshu Cheng,9 Pierre Zalloua,10 Xiping Xu,11 Yong Huo,12 Gang Li1 1Department of Neurology, East Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China; 2State Key Laboratory of Natural Medicines, Research Center of Biostatistics and Computational Pharmacy, China Pharmaceutical University, Nanjing, People’s Republic of China; 3Shenzhen Evergreen Medical Institute, Guangzhou, People’s Republic of China; 4People’s Hospital of Rongcheng, Rongcheng, Shandong, People’s Republic of China; 5Health and Family Planning Commission, Rongcheng, Shandong, People’s Republic of China; 6Department of Neurosurgery, Lianyungang Clinical College of Nanjing Medical University, Lianyungang, People’s Republic of China; 7Lianyungang Health Committee, Lianyungang, Jiangsu, People’s Republic of China; 8Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, People’s Republic of China; 9Department of Cardiovascular Medicine, The Second Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China; 10School of Medicine, Lebanese American University, Beirut, Lebanon; 11Division of Nephrology, Nanfang Hospital, Southern Medical University; National Clinical Research Center for Kidney Disease; State Key Laboratory of Organ Failure Research; Guangdong Provincial Institute of Nephrology, Guangzhou, People’s Republic of China; 12Department of Cardiology, Peking University First Hospital, Beijing, People’s Republic of ChinaCorrespondence: Gang Li 1800 Yuntai Road, Shanghai, 200123, People’s Republic of ChinaTel +86-21-38804518-22107Fax +86-21-5879

Edible salt is essential to the health of humans and serves as a seasoning universally. Besides chloride, edible salt also contains other anions such as bromide, fluoride, sulfate, and carbonate due to incomplete removal during raw salt refinement. In a household cooking (e.g., soup making) process, a chlorine/monochloramine residual in tap water could react with bromide in edible salt and organic matter in food (e.g., rice, wheat) to form numerous brominated disinfection byproducts (Br-DBPs) at significant levels, which might induce adverse health effects to human beings. In this study, we solicited 20 edible salts of different types (i.e., sea salts, well and rock salts, lake salts, and bamboo salts) from nine countries and determined their bromide levels to be 67-375 mg/kg, with an average level of 173 mg/kg. A total of 25 polar Br-DBPs were detected and identified with structures/formulae in cooking water samples using ultra performance liquid chromatography/electrospray ionization-triple quadruple mass spectrometry (UPLC/ESI-tqMS) and high-resolution mass spectrometry. Effects of cooking conditions (e.g., disinfectant type and level, edible salt dose, organic matter type and dose, sequence and time interval of adding organic matter and salt, etc.) on the formation of polar Br-DBPs were investigated, and optimized cooking conditions with minimized formation of polar Br-DBPs were determined. Further aided with an Hep G2 cell cytotoxicity assay, it was found that the overall cytotoxicity of chlorinated and chloraminated cooking water samples prepared after cooking condition optimization was reduced by 57% and 22%, respectively, compared with those prepared before cooking condition optimization. (C) 2020 Elsevier Ltd. All rights reserved.

Edible salt is essential to the health of humans and serves as a seasoning universally. Besides chloride, edible salt also contains other anions such as bromide, fluoride, sulfate, and carbonate due to incomplete removal during raw salt refinement. In a household cooking (e.g., soup making) process, a chlorine/monochloramine residual in tap water could react with bromide in edible salt and organic matter in food (e.g., rice, wheat) to form numerous brominated disinfection byproducts (Br-DBPs) at significant levels, which might induce adverse health effects to human beings. In this study, we solicited 20 edible salts of different types (i.e., sea salts, well and rock salts, lake salts, and bamboo salts) from nine countries and determined their bromide levels to be 67-375 mg/kg, with an average level of 173 mg/kg. A total of 25 polar Br-DBPs were detected and identified with structures/formulae in cooking water samples using ultra performance liquid chromatography/electrospray ionization-triple quadruple mass spectrometry (UPLC/ESI-tqMS) and high-resolution mass spectrometry. Effects of cooking conditions (e.g., disinfectant type and level, edible salt dose, organic matter type and dose, sequence and time interval of adding organic matter and salt, etc.) on the formation of polar Br-DBPs were investigated, and optimized cooking conditions with minimized formation of polar Br-DBPs were determined. Further aided with an Hep G2 cell cytotoxicity assay, it was found that the overall cytotoxicity of chlorinated and chloraminated cooking water samples prepared after cooking condition optimization was reduced by 57% and 22%, respectively, compared with those prepared before cooking condition optimization. (C) 2020 Elsevier Ltd. All rights reserved.

Edible salt is essential to the health of humans and serves as a seasoning universally. Besides chloride, edible salt also contains other anions such as bromide, fluoride, sulfate, and carbonate due to incomplete removal during raw salt refinement. In a household cooking (e.g., soup making) process, a chlorine/monochloramine residual in tap water could react with bromide in edible salt and organic matter in food (e.g., rice, wheat) to form numerous brominated disinfection byproducts (Br-DBPs) at significant levels, which might induce adverse health effects to human beings. In this study, we solicited 20 edible salts of different types (i.e., sea salts, well and rock salts, lake salts, and bamboo salts) from nine countries and determined their bromide levels to be 67-375 mg/kg, with an average level of 173 mg/kg. A total of 25 polar Br-DBPs were detected and identified with structures/formulae in cooking water samples using ultra performance liquid chromatography/electrospray ionization-triple quadruple mass spectrometry (UPLC/ESI-tqMS) and high-resolution mass spectrometry. Effects of cooking conditions (e.g., disinfectant type and level, edible salt dose, organic matter type and dose, sequence and time interval of adding organic matter and salt, etc.) on the formation of polar Br-DBPs were investigated, and optimized cooking conditions with minimized formation of polar Br-DBPs were determined. Further aided with an Hep G2 cell cytotoxicity assay, it was found that the overall cytotoxicity of chlorinated and chloraminated cooking water samples prepared after cooking condition optimization was reduced by 57% and 22%, respectively, compared with those prepared before cooking condition optimization. (C) 2020 Elsevier Ltd. All rights reserved.

Recent studies have revealed that feed-forward loops (FFLs) as regulatory motifs have synergistic roles in cellular systems and their disruption may cause diseases including cancer. FFLs may include two regulators such as transcription factors (TFs) and microRNAs (miRNAs). In this study, we extensively investigated TF and miRNA regulation pairs, their FFLs, and TF-miRNA mediated regulatory networks in two major types of testicular germ cell tumors (TGCT): seminoma (SE) and non-seminoma (NSE). Specifically, we identified differentially expressed mRNA genes and miRNAs in 103 tumors using the transcriptomic data from The Cancer Genome Atlas. Next, we determined significantly correlated TF-gene/miRNA and miRNA-gene/TF pairs with regulation direction. Subsequently, we determined 288 and 664 dysregulated TF-miRNA-gene FFLs in SE and NSE, respectively. By constructing dysregulated FFL networks, we found that many hub nodes (12 out of 30 for SE and 8 out of 32 for NSE) in the top ranked FFLs could predict subtype-classification (Random Forest classifier, average accuracy ≥90%). These hub molecules were validated by an independent dataset. Our network analysis pinpointed several SE-specific dysregulated miRNAs (miR-200c-3p, miR-25-3p, and miR-302a-3p) and genes (EPHA2, JUN, KLF4, PLXDC2, RND3, SPI1, and TIMP3) and NSE-specific dysregulated miRNAs (miR-367-3p, miR-519d-3p, and miR-96-5p) and genes (NR2F1 and NR2F2). This study is the first systematic investigation of TF and miRNA regulation and their co-regulation in two major TGCT subtypes.

Na Qi,1,2 Yu Zhang,3 Xing Tang,3 Aimin Li1 1Integrated Hospital of Traditional Chinese Medicine, Southern Medical University, Guangzhou 510315, People’s Republic of China; 2Department of Pharmacy, Guilin Medical University, Guilin 541004, People’s Republic of China; 3Department of Pharmaceutics, Shenyang Pharmaceutical University, Shenyang 110016, People’s Republic of ChinaCorrespondence: Aimin Li; Xing Tang Email liaimin2005@163.com; tanglab@126.comBackground: Cationic and anionic polymer-modified nanoparticles offer promising properties for the drug and gene delivery. Our study uses cationic/anionic polyelectrolyte coated vesicular phospholipid gels (VPGs) loaded with cytarabine (Ara-C) that enhance in vitro and in vivo anti-glioma effects.Methods: Sodium cholesteryl sulfate (SCS) or octadecylamine (ODA) incorporated in a phospholipids phase were used to prepare charged VPGs, and cationic ϵ-polylysine (PLL) coated VPGs (PLL-SCS VPGs) and anionic γ-polyglutamic acid (PGA) coated VPGs (PGA-ODA VPGs) were prepared via electrostatic interactions, respectively. The morphology, particle size, zeta potential, rheology properties, and in vitro release were then characterized. The in vitro cytotoxicity and cellular uptake were evaluated on U87-MG glioma cells. The in vivo antitumor effects were studied on BALB/c nude mice bearing a right flank U87-MG glioma model.Results: The TEM images and physicochemical properties of cationic/anionic polyelectrolyte coated VPGs exhibited that polymers covered on the vesicular surface. The results of rheologic property analysis showed that cationic/anionic polyelectrolyte coated VPGs enhanced the viscosity of uncoated VPGs. The in vitro release experiments revealed that cationic/anionic polyelectrolyte coated VPGs kept Ara-C sustained release up to 18 days. Specially, compared with PLL-SCS VPGs, PGA-ODA VPGs demonstrated higher in vitro cytotoxicity and cellular uptake efficiency in U87-MG glioma

Recent studies have revealed that feed-forward loops (FFLs) as regulatory motifs have synergistic roles in cellular systems and their disruption may cause diseases including cancer. FFLs may include two regulators such as transcription factors (TFs) and microRNAs (miRNAs). In this study, we extensively investigated TF and miRNA regulation pairs, their FFLs, and TF-miRNA mediated regulatory networks in two major types of testicular germ cell tumors (TGCT): seminoma (SE) and non-seminoma (NSE). Specifically, we identified differentially expressed mRNA genes and miRNAs in 103 tumors using the transcriptomic data from The Cancer Genome Atlas. Next, we determined significantly correlated TF-gene/miRNA and miRNA-gene/TF pairs with regulation direction. Subsequently, we determined 288 and 664 dysregulated TF-miRNA-gene FFLs in SE and NSE, respectively. By constructing dysregulated FFL networks, we found that many hub nodes (12 out of 30 for SE and 8 out of 32 for NSE) in the top ranked FFLs could predict subtype-classification (Random Forest classifier, average accuracy ≥90%). These hub molecules were validated by an independent dataset. Our network analysis pinpointed several SE-specific dysregulated miRNAs (miR-200c-3p, miR-25-3p, and miR-302a-3p) and genes (EPHA2, JUN, KLF4, PLXDC2, RND3, SPI1, and TIMP3) and NSE-specific dysregulated miRNAs (miR-367-3p, miR-519d-3p, and miR-96-5p) and genes (NR2F1 and NR2F2). This study is the first systematic investigation of TF and miRNA regulation and their co-regulation in two major TGCT subtypes.

This work presents a new extraction material, namely, Q-100, based on hypercrosslinked magnetic particles, which was tested in dispersive solid-phase extraction for a group of sweeteners from environmental samples. The hypercrosslinked Q-100 magnetic particles had the advantage of suitable pore size distribution and high surface area, and showed good retention behavior toward sweeteners. Different dispersive solid-phase extraction parameters such as amount of magnetic particles or extraction time were optimized. Under optimum conditions, Q-100 showed suitable apparent recovery, ranging in the case of river water sample from 21 to 88% for all the sweeteners, except for alitame (12%). The validated method based on dispersive solid-phase extraction using Q-100 followed by liquid chromatography with tandem mass spectrometry provided good linearity and limits of quantification between 0.01 and 0.1Â ÎŒg/L. The method was applied to analyze samples from river water and effluent wastewater, and four sweeteners (acesulfame, saccharin, cyclamate, and sucralose) were found in both types of sample.

Yonggang Zhang,1 Tingrui Ge,1 Ping Xiang,1 Haibing Mao,1 Shumin Tang,1 Aimin Li,2 Lin Lin,3 Yinting Wei4 1Department of Colorectal Surgery, The First People’s Hospital of Lianyungang, Lianyungang 222002, China; 2Department of Neurosurgery, The First People’s Hospital of Lianyungang, Lianyungang 222002, China; 3Department of Gastroenterology, Jiangsu Province Hospital, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, China; 4Department of Gastroenterology, Lianyungang TCM Hospital Affiliated to Nanjing University of Chinese Medicine, Lianyungang 222000, China Purpose: To investigate the therapeutic effects of protease-activated receptor 2 (PAR-2) agonist SLIGRL-NH2 on loperamide-induced Sprague-Dawley (SD) rat constipation animal models. Materials and methods: Loperamide was injected subcutaneously to induce constipation twice a day for 3 days. SD rats (n = 30) were randomly divided into five groups: non-constipation group (control, n = 6), constipation group (constipation, n = 6), constipation + SLIGRL-NH2 low-dosage group (SLIGRL-NH2 low, n=6), constipation + SLIGRL-NH2 high-dosage group (SLIGRL-NH2 high, n = 6), and constipation + prucalopride (positive control, n = 6). The SLIGRL-NH2 low group and SLIGRL-NH2 high group were administered with 2.5 μmol/kg and 5 μmol/kg SLIGRL-NH2, respectively, and the prucalopride group received 2 mg/kg prucalopride. The control and constipation group received 1× PBS under the same pattern. SLIGRL-NH2 and prucalopride were orally administrated once daily for 7 days. On the final day of oral administration, food intake, water intake, the number of stool pellets, weight, and fecal water content was calculated; moreover, the colons of rats in different groups were collected and histological features were examined by hematoxylin and eosin staining; furthermore, the expression of anoctamin-1 was determined by Immunohistochemical methods, and the expressions of c-ki

Natural organic matter (NOM) is present in source water worldwide, and it is composed of numerous organic compounds produced in natural processes. NOM has been drawing increasing concerns because it not only affects drinking water treatment on many aspects (e.g., the performance of treatment techniques, necessity of advanced treatment, the dose of treatment chemicals, etc.), but also acts as the main precursor of hazardous drinking water disinfection byproducts (DBPs). Accordingly, to optimize treatment processes and to control the formation of DBPs, it is vital to analyze and characterize NOM for a better understanding of its property and reactivity. In this review, a summary of methods used in NOM analysis and characterization during drinking water treatment was provided, including analysis methods for general parameters (e.g., dissolved organic carbon, dissolved organic nitrogen, (specific) ultraviolet absorbance, fluorescence, polarity, and zeta potential) and characterization methods for elemental and structural identification (e.g., elemental analysis, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, pyrolysis-gas chromatography/mass spectrometry, liquid chromatography/mass spectrometry, and Fourier transform ion cyclotron resonance mass spectrometry). In addition, preparation and pretreatment methods for NOM were also covered in this review, including isolation and concentration methods (reverse osmosis, pressure-reducing evaporation, freeze-drying, and electrodialysis) as well as fractionation methods (size exclusion chromatography, resin fractionation, membrane filtration, polarity rapid assessment, field-flow fractionation, and reversed-phase high performance liquid chromatography). © 2016 Elsevier B.V.

Natural organic matter (NOM) is present in source water worldwide, and it is composed of numerous organic compounds produced in natural processes. NOM has been drawing increasing concerns because it not only affects drinking water treatment on many aspects (e.g., the performance of treatment techniques, necessity of advanced treatment, the dose of treatment chemicals, etc.), but also acts as the main precursor of hazardous drinking water disinfection byproducts (DBPs). Accordingly, to optimize treatment processes and to control the formation of DBPs, it is vital to analyze and characterize NOM for a better understanding of its property and reactivity. In this review, a summary of methods used in NOM analysis and characterization during drinking water treatment was provided, including analysis methods for general parameters (e.g., dissolved organic carbon, dissolved organic nitrogen, (specific) ultraviolet absorbance, fluorescence, polarity, and zeta potential) and characterization methods for elemental and structural identification (e.g., elemental analysis, Fourier transform infrared spectroscopy, nuclear magnetic resonance spectroscopy, pyrolysis-gas chromatography/mass spectrometry, liquid chromatography/mass spectrometry, and Fourier transform ion cyclotron resonance mass spectrometry). In addition, preparation and pretreatment methods for NOM were also covered in this review, including isolation and concentration methods (reverse osmosis, pressure-reducing evaporation, freeze-drying, and electrodialysis) as well as fractionation methods (size exclusion chromatography, resin fractionation, membrane filtration, polarity rapid assessment, field-flow fractionation, and reversed-phase high performance liquid chromatography). © 2016 Elsevier B.V.

Prion-like propagation of tau aggregation might underlie the stereotyped progression of neurodegenerative tauopathies. True prions stably maintain unique conformations ("strains") in vivo that link structure to patterns of pathology. We now find that tau meets this criterion. Stably expressed tau repeat domain indefinitely propagates distinct amyloid conformations in a clonal fashion in culture. Reintroduction of tau from these lines into naive cells reestablishes identical clones. We produced two strains in vitro that induce distinct pathologies in vivo as determined by successive inoculations into three generations of transgenic mice. Immunopurified tau from these mice recreates the original strains in culture. We used the cell system to isolate tau strains from 29 patients with 5 different tauopathies, finding that different diseases are associated with different sets of strains. Tau thus demonstrates essential characteristics of a prion. This might explain the phenotypic diversity of tauopathies and could enable more effective diagnosis and therapy.

A multiresolution approach is presented for NURBS curve fairing based on nonuniform semiorthogonal B-spline wavelets built. This method provides greater flexibility and applicability than uniform B-spline wavelets for multiresolution curve fairing. An example is presented to validate effectiveness of this multiresolution fairing method. Furthermore, the algorithm can be easily applied to NURBS curves in three dimensions as well as in two. © (2011) Trans Tech Publications.

A multiresolution approach is presented for NURBS curve fairing based on nonuniform semiorthogonal B-spline wavelets built. This method provides greater flexibility and applicability than uniform B-spline wavelets for multiresolution curve fairing. An example is presented to validate effectiveness of this multiresolution fairing method. Furthermore, the algorithm can be easily applied to NURBS curves in three dimensions as well as in two. © (2011) Trans Tech Publications.

A multiresolution approach is presented for NURBS curve fairing based on nonuniform semiorthogonal B-spline wavelets built. This method provides greater flexibility and applicability than uniform B-spline wavelets for multiresolution curve fairing. An example is presented to validate effectiveness of this multiresolution fairing method. Furthermore, the algorithm can be easily applied to NURBS curves in three dimensions as well as in two. © (2011) Trans Tech Publications.